Compounds, compositions, and methods for preventing metastasis of cancer cells

ABSTRACT

Disclosed are methods for preventing metastasis of cancer cells. The disclosed compounds can be used to prevent the spread of tumor or other types of cancer cells.

PRIORITY

This application claims the benefit of U.S. Provisional Application Ser.No. 61/223,260 filed on Jun. 6, 2009, the entire disclosure of whichapplication is incorporated herein by reference.

FIELD

Disclosed are compounds that can prevent the metastasis of canceroustumors and/or reduce tumor growth. Further disclosed are compositions,especially pharmaceutical compositions for preventing metastasis ofmalignant tumors and reducing tumor growth. Included within thecompositions are combined therapy compositions for use in treatingcancer. Still further disclosed are methods for preventing metastasis ofcancerous tumors and other cancer cells. As such, the disclosedcompounds can be used to aid in the prevention of cancerous growth in asubject having cancer or diagnosed with cancer.

BACKGROUND

One of the most frightening aspects of cancer is its ability to spread,or metastasize. Initially, cancer cells are found grouped togetherthereby forming one or more tumors. After formation of the primarytumor, cancer cells can gain the ability to separate from the originaltumor and travel to other areas of the body. Lung cancer cells that takeup in the liver and form tumors are still lung cancer cells. Thus, thepropensity for one particular form of cancer to metastasize is dependenton many factors, including type of cancer; however, the overall processof how cells begin the process of metastasis is still not completelyunder stood.

If a single localized tumor is discovered before it has had a chance tometastasize, then the prognosis of patient survival is higher. This isbecause the tumor can be effectively excised or destroyed by radiationor chemotherapy. There is, therefore, a difference between tumor growthand metastasis of the tumor cells; the first does not always lead to theother. Cancers that have metastasized, however, are difficult to curebecause the extent to which they have spread throughout the body issometimes not discernable.

In order to metastasize, a cancer cell must break away from its tumorand invade either the circulatory or lymph system. The free cells arethen carried to a new location where they establish themselves. Althoughthe body has natural safeguards that prevent cell from surviving afterbeing detached from their natural location, some cancer cells have theability to over come these safeguards. Therefore, if metastasis isstopped or significantly reduced, the extent of cancer can be determinedand subsequently treated. As such, a follow up treatment to cancertherapy wherein a tumor has been excised or radiation/chemotherapy hasbeen used, would be the treatment of the patient to ananti-metastasizing agent. There is a long felt need for methods ofpreventing cancer cell metastasis.

The growth of primary tumors also presents a challenge to treatment. Ifthe growth of a primary tumor goes unchecked, the initial tumor can growto a size that adversely effects organ function at the primary site andin nearby tissues. Metastases of the primary tumor are also more likelyif the primary tumor's growth is uncontrolled. There is a need formethods of slowing or preventing tumor growth.

SUMMARY

Disclosed herein are compounds, compositions, and methods for preventingmetastasis of cancer cells. Further disclosed are compounds,compositions, and methods that can be used for treating cancer,including combination therapy compositions wherein the disclosedcompounds are combined with one or more cancer treatment drugs or otherchemothrerapeutic agents.

Additional advantages will be set forth in part in the description thatfollows, and in part will be obvious from the description, or may belearned by practice of the aspects described below. The advantagesdescribed below will be realized and attained by means of the elementsand combinations particularly pointed out in the appended claims. It isto be understood that both the foregoing general description and thefollowing detailed description are exemplary and explanatory only andare not restrictive.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 depicts a graph showing the increase in tumor volume with time ofrenal cell carcinoma tumors (Renca) in mice; vehicle control (□);100,000 IU/dose of IL-2 twice daily (⋄); 40 mg/kg of D91 twice daily(Δ); 100,000 IU/dose of IL-2 and 40 mg/kg of D91 twice daily (◯).

FIG. 2 is a histogram of the volume of renal cell carcinoma tumors(Renca) in mice at day 25; vehicle control (A); 40 mg/kg of D91 twicedaily (B); 100,000 IU/dose of IL-2 twice daily (C); 100,000 IU/dose ofIL-2 and 40 mg/kg of D91 twice daily (D).

FIG. 3 depicts a graph showing the increase in tumor volume with time ofB16 melanoma tumors in mice; vehicle control (□); 300,000 IU/dose ofIL-2 twice daily (⋄); 300,000 IU/dose of IL-2 and 40 mg/kg of D91 twicedaily (◯).

FIG. 4 depicts histogram of the volume of B16 melanoma tumors in mice atday 22; vehicle control (A); 300,000 IU/dose of IL-2 twice daily (B);300,000 IU/dose of IL-2 and 40 mg/kg of D91 twice daily (C).

FIG. 5 depicts a graph of the body weight of mice with B16 melanomatumors; vehicle control (□); 100,000 IU/dose of IL-2 twice daily (⋄);300,000 IU/dose of IL-2 twice daily (Δ); 100,000 IU/dose of IL-2 and 40mg/kg of D91 twice daily (◯); and 300,000 IU/dose of IL-2 and 40 mg/kgof D91 twice daily (▪).

FIG. 6 depicts a graph showing the increase in tumor volume with time ofB16 melanoma tumors in mice; vehicle control (□); 100,000 IU/dose ofIL-2 twice daily (⋄); and 100,000 IU/dose of IL-2 and 40 mg/kg of D91twice daily (◯).

FIG. 7 depicts histogram of the volume of B16 melanoma tumors in mice atday 22; vehicle control (A); 100,000 IU/dose of IL-2 twice daily (B);and 100,000 IU/dose of IL-2 and 40 mg/kg of D91 twice daily (C).

FIG. 8 depicts a graph showing the increase in tumor volume with time ofB16 melanoma tumors in mice; vehicle control (□); and 40 mg/kg of D91twice daily (◯).

FIG. 9 depicts the size of pancreatic tumors orthotopically implanted inNCr nu/nu mice: vehicle control (⋄); 100 mg/kg gemcitabine, i.p. twiceweekly (X); 150 mg/kg gemcitabine, i.p. twice weekly (□); 20 mg/kg D91twice daily (◯); 100 mg/kg gemcitabine, i.p. twice weekly and 20 mg/kgD91 twice daily (*); and 150 mg/kg gemcitabine, i.p. twice weekly and 20mg/kg D91 twice daily (Δ).

DETAILED DESCRIPTION

In this specification and in the claims that follow, reference will bemade to a number of terms, which shall be defined to have the followingmeanings:

All percentages, ratios and proportions herein are by weight, unlessotherwise specified. All temperatures are in degrees Celsius (° C.)unless otherwise specified.

By “pharmaceutically acceptable” is meant a material that is notbiologically or otherwise undesirable, i.e., the material can beadministered to an individual along with the relevant active compoundwithout causing clinically unacceptable biological effects orinteracting in a deleterious manner with any of the other components ofthe pharmaceutical composition in which it is contained.

Throughout the description and claims of this specification the word“comprise” and other forms of the word, such as “comprising” and“comprises,” means including but not limited to, and is not intended toexclude, for example, other additives, components, integers, or steps.

As used in the description and the appended claims, the singular forms“a,” “an,” and “the” include plural referents unless the context clearlydictates otherwise. Thus, for example, reference to “a composition”includes mixtures of two or more such compositions, reference to “aphenylsulfamic acid” includes mixtures of two or more suchphenylsulfamic acids, reference to “the compound” includes mixtures oftwo or more such compounds, and the like.

“Optional” or “optionally” means that the subsequently described eventor circumstance can or cannot occur, and that the description includesinstances where the event or circumstance occurs and instances where itdoes not.

Ranges can be expressed herein as from “about” one particular value,and/or to “about” another particular value. When such a range isexpressed, another aspect includes from the one particular value and/orto the other particular value. Similarly, when values are expressed asapproximations, by use of the antecedent “about,” it will be understoodthat the particular value forms another aspect. It will be furtherunderstood that the endpoints of each of the ranges are significant bothin relation to the other endpoint, and independently of the otherendpoint. It is also understood that there are a number of valuesdisclosed herein, and that each value is also herein disclosed as“about” that particular value in addition to the value itself. Forexample, if the value “10” is disclosed, then “about 10” is alsodisclosed. It is also understood that when a value is disclosed, then“less than or equal to” the value, “greater than or equal to the value,”and possible ranges between values are also disclosed, as appropriatelyunderstood by the skilled artisan. For example, if the value “10” isdisclosed, then “less than or equal to 10” as well as “greater than orequal to 10” is also disclosed. It is also understood that throughoutthe application data are provided in a number of different formats andthat this data represent endpoints and starting points and ranges forany combination of the data points. For example, if a particular datapoint “10” and a particular data point “15” are disclosed, it isunderstood that greater than, greater than or equal to, less than, lessthan or equal to, and equal to 10 and 15 are considered disclosed aswell as between 10 and 15. It is also understood that each unit betweentwo particular units are also disclosed. For example, if 10 and 15 aredisclosed, then 11, 12, 13, and 14 are also disclosed.

As used herein, the terms “manage,” “managing” and “management” refer tothe beneficial effects that a subject derives from administration of aprophylactic or therapeutic agent, which does not result in a cure ofthe disease or diseases. In certain embodiments, a subject isadministered one or more prophylactic or therapeutic agents to “manage”a disease so as to prevent the progression or worsening of the diseaseor diseases.

As used herein, the terms “prevent”, “preventing” and “prevention” referto the methods to avert or avoid a disease or disorder or delay therecurrence or onset of one or more symptoms of a disorder in a subjectresulting from the administration of a prophylactic agent.

As used herein, the term “in combination” refers to the use of more thanone prophylactic and/or therapeutic agents. The use of the term “incombination” does not restrict the order in which prophylactic and/ortherapeutic agents are administered to a subject with a disorder, e.g.,hyperproliferative cell disorder, especially cancer. A firstprophylactic or therapeutic agent can be administered prior to (e.g., 1minute, 5 minutes, 15 minutes, 30 minutes, 45 minutes, 1 hour, 2 hours,4 hours, 6 hours, 12 hours, 24 hours, 48 hours, 72 hours, 96 hours, 1week, 2 weeks, 3 weeks, 4 weeks, 5 weeks, 6 weeks, 8 weeks, or 12 weeksbefore), concomitantly with, or subsequent to (e.g., 1 minute, 5minutes, 15 minutes, 30 minutes, 45 minutes, 1 hour, 2 hours, 4 hours, 6hours, 12 hours, 24 hours, 48 hours, 72 hours, 96 hours, 1 week, 2weeks, 3 weeks, 4 weeks, 5 weeks, 6 weeks, 8 weeks, or 12 weeks after)the administration of a second prophylactic or therapeutic agent to asubject which had, has, or is susceptible to a disorder. Theprophylactic or therapeutic agents are administered to a subject in asequence and within a time interval such that the agent of the presentdisclosure can act together with the other agent to provide an increasedbenefit than if they were administered otherwise. Any additionalprophylactic or therapeutic agent can be administered in any order withthe other additional prophylactic or therapeutic agents

As used herein, the terms “administer” when used to describe the dosageof a compound, means a single dose or multiple doses of the compound.

As used herein, the term “cancer treatment” means any treatment forcancer known in the art including, but not limited to, chemotherapy andradiation therapy.

As used herein, “tumor cells” means both cells derived from tumors,including malignant tumors, and cells immortalized in vitro.“Normal”cells refer to cells with normal growth characteristics that donot show abnormal proliferation.

As used herein, the terms “an individual identified as having cancer”and “cancer patient” are used interchangeably and are meant to refer toan individual who has been diagnosed as having cancer. There arenumerous well known means for identifying an individual who has cancer.In some embodiments, a cancer diagnosis is made or confirmed using PETimaging. Some embodiments of the present disclosure comprise the step ofidentifying individuals who have cancer.

As used herein, the term “therapeutically effective amount” is meant torefer to an amount of an active agent or combination of agents effectiveto ameliorate or prevent the symptoms, shrink tumor size, or prolong thesurvival of the patient being treated. Determination of atherapeutically effective amount is well within the capabilities ofthose skilled in the art, especially in light of the detailed disclosureprovided herein.

As used herein the term “inhibit” or “inhibiting” refers to astatistically significant and measurable reduction in activity,preferably a reduction of at least about 10% versus control, morepreferably a reduction of about 50% or more, still more preferably areduction of about 80% or more.

As used herein the term “increase” or “enhancing” refers to astatistically significant and measurable increase in activity,preferably an increase of at least about 10% versus control, morepreferably an increase of about 50% or more, still more preferably anincrease of about 80% or more.

The term “prevent” or “preventing” when used in relation to a condition,such as a local recurrence (e.g., pain), a disease such as cancer, asyndrome complex such as heart failure or any other medical condition,is well understood in the art, and includes administration of acomposition which reduces the frequency of, or delays the onset of,symptoms of a medical condition in a subject relative to a subject whichdoes not receive the composition. Thus, prevention of cancer includes,for example, reducing the number of detectable cancerous growths in apopulation of patients receiving a prophylactic treatment relative to anuntreated control population, and/or delaying the appearance ofdetectable cancerous growths in a treated population versus an untreatedcontrol population, e.g., by a statistically and/or clinicallysignificant amount. Prevention of an infection includes, for example,reducing the number of diagnoses of the infection in a treatedpopulation versus an untreated control population, and/or delaying theonset of symptoms of the infection in a treated population versus anuntreated control population. Prevention of pain includes, for example,reducing the magnitude of, or alternatively delaying, pain sensationsexperienced by subjects in a treated population versus an untreatedcontrol population.

The terms “treatment”, “treating”, “treat”, and the like, refer toobtaining a desired pharmacologic and/or physiologic effect. The effectmay be prophylactic in terms of completely or partially preventing adisease or symptom thereof and/or may be therapeutic in terms of apartial or complete cure for a disease and/or adverse affectattributable to the disease. “Treatment”, as used herein, covers anytreatment of a disease in a mammal, particularly in a human, andincludes: (a) preventing the disease from occurring in a subject whichmay be predisposed to the disease but has not yet been diagnosed ashaving it; (b) inhibiting the disease, i.e., arresting its development;and (c) relieving the disease, i.e., causing regression of the diseaseand/or relieving one or more disease symptoms. “Treatment” is also meantto encompass delivery of an agent in order to provide for apharmacologic effect, even in the absence of a disease or condition. Forexample, “treatment” encompasses delivery of a receptor modulator thatcan provide for enhanced or desirable effects in the subject (e.g.,reduction of pathogen load, beneficial increase in a physiologicalparameter of the subject, reduction of disease symptoms, etc.).

Throughout this application, various publications are referenced. Thedisclosures of these publications in their entireties are herebyincorporated by reference into this application in order to more fullydescribe the state of the art to which this pertains. The referencesdisclosed are also individually and specifically incorporated byreference herein for the material contained in them that is discussed inthe sentence in which the reference is relied upon

The following chemical hierarchy is used throughout the specification todescribe and enable the scope of the present disclosure and toparticularly point out and distinctly claim the units which comprise thecompounds of the present disclosure, however, unless otherwisespecifically defined, the terms used herein are the same as those of theartisan of ordinary skill. The term “hydrocarbyl” stands for any carbonatom-based unit (organic molecule), said units optionally containing oneor more organic functional group, including inorganic atom comprisingsalts, inter alia, carboxylate salts, quaternary ammonium salts. Withinthe broad meaning of the term “hydrocarbyl” are the classes “acyclichydrocarbyl” and “cyclic hydrocarbyl” which terms are used to dividehydrocarbyl units into cyclic and non-cyclic classes.

As it relates to the following definitions, “cyclic hydrocarbyl” unitscan comprise only carbon atoms in the ring (i.e., carbocyclic and arylrings) or can comprise one or more heteroatoms in the ring (i.e.,heterocyclic and heteroaryl rings). For “carbocyclic” rings the lowestnumber of carbon atoms in a ring are 3 carbon atoms; cyclopropyl. For“aryl” rings the lowest number of carbon atoms in a ring are 6 carbonatoms; phenyl. For “heterocyclic” rings the lowest number of carbonatoms in a ring is 1 carbon atom; diazirinyl. Ethylene oxide comprises 2carbon atoms and is a C₂ heterocycle. For “heteroaryl” rings the lowestnumber of carbon atoms in a ring is 1 carbon atom; 1,2,3,4-tetrazolyl.The following is a non-limiting description of the terms “acyclichydrocarbyl” and “cyclic hydrocarbyl” as used herein.

A. Substituted and unsubstituted acyclic hydrocarbyl:

-   -   For the purposes of the present disclosure the term “substituted        and unsubstituted acyclic hydrocarbyl” encompasses 3 categories        of units:

-   1) linear or branched alkyl, non-limiting examples of which include,    methyl (C₁), ethyl (C₂), n-propyl (C₃), iso-propyl (C₃), n-butyl    (C₄), sec-butyl (C₄), iso-butyl (C₄), tert-butyl (C₄), and the like;    substituted linear or branched alkyl, non-limiting examples of which    includes, hydroxymethyl (C₁), chloromethyl (C₁), trifluoromethyl    (C₁), aminomethyl (C₁), 1-chloroethyl (C₂), 2-hydroxyethyl (C₂),    1,2-difluoroethyl (C₂), 3-carboxypropyl (C₃), and the like.

-   2) linear or branched alkenyl, non-limiting examples of which    include, ethenyl (C₂), 3-propenyl (C₃), 1-propenyl (also    2-methylethenyl) (C₃), isopropenyl (also 2-methylethen-2-yl) (C₃),    buten-4-yl (C₄), and the like; substituted linear or branched    alkenyl, non-limiting examples of which include, 2-chloroethenyl    (also 2-chlorovinyl) (C₂), 4-hydroxybuten-1-yl (C₄),    7-hydroxy-7-methyloct-4-en-2-yl (C₉),    7-hydroxy-7-methyloct-3,5-dien-2-yl (C₉), and the like.

-   3) linear or branched alkynyl, non-limiting examples of which    include, ethynyl (C₂), prop-2-ynyl (also propargyl) (C₃),    propyn-1-yl (C₃), and 2-methyl-hex-4-yn-1-yl (C₇); substituted    linear or branched alkynyl, non-limiting examples of which include,    5-hydroxy-5-methylhex-3-ynyl (C₇), 6-hydroxy-6-methylhept-3-yn-2-yl    (C₈), 5-hydroxy-5-ethylhept-3-ynyl (C₉), and the like.    B. Substituted and unsubstituted cyclic hydrocarbyl:    -   For the purposes of the present disclosure the term “substituted        and unsubstituted cyclic hydrocarbyl” encompasses 5 categories        of units:

-   1) The term “carbocyclic” is defined herein as “encompassing rings    comprising from 3 to 20 carbon atoms, wherein the atoms which    comprise said rings are limited to carbon atoms, and further each    ring can be independently substituted with one or more moieties    capable of replacing one or more hydrogen atoms.” The following are    non-limiting examples of “substituted and unsubstituted carbocyclic    rings” which encompass the following categories of units:    -   i) carbocyclic rings having a single substituted or        unsubstituted hydrocarbon ring, non-limiting examples of which        include, cyclopropyl (C₃), 2-methyl-cyclopropyl (C₃),        cyclopropenyl (C₃), cyclobutyl (C₄), 2,3-dihydroxycyclobutyl        (C₄), cyclobutenyl (C₄), cyclopentyl (C₅), cyclopentenyl (C₅),        cyclopentadienyl (C₅), cyclohexyl (C₆), cyclohexenyl (C₆),        cycloheptyl (C₇), cyclooctanyl (C₈), 2,5-dimethylcyclopentyl        (C₅), 3,5-dichlorocyclohexyl (C₆), 4-hydroxycyclohexyl (C₆), and        3,3,5-trimethylcyclohex-1-yl (C₆).    -   ii) carbocyclic rings having two or more substituted or        unsubstituted fused hydrocarbon rings, non-limiting examples of        which include, octahydropentalenyl (C₈), octahydro-1H-indenyl        (C₉), 3a,4,5,6,7,7a-hexahydro-3H-inden-4-yl (C₉),        decahydroazulenyl (C₁₀).    -   iii) carbocyclic rings which are substituted or unsubstituted        bicyclic hydrocarbon rings, non-limiting examples of which        include, bicyclo-[2.1.1]hexanyl, bicyclo[2.2.1]heptanyl,        bicyclo[3.1.1]heptanyl, 1,3-dimethyl[2.2.1]heptan-2-yl,        bicyclo[2.2.2]octanyl, and bicyclo[3.3.3]undecanyl.

-   2) The term “aryl” is defined herein as “units encompassing at least    one phenyl or naphthyl ring and wherein there are no heteroaryl or    heterocyclic rings fused to the phenyl or naphthyl ring and further    each ring can be independently substituted with one or more moieties    capable of replacing one or more hydrogen atoms.” The following are    non-limiting examples of “substituted and unsubstituted aryl rings”    which encompass the following categories of units:    -   i) C₆ or C₁₀ substituted or unsubstituted aryl rings; phenyl and        naphthyl rings whether substituted or unsubstituted,        non-limiting examples of which include, phenyl (C₆),        naphthylen-1-yl (C₁₀), naphthylen-2-yl (C₁₀), 4-fluorophenyl        (C₆), 2-hydroxyphenyl (C₆), 3-methylphenyl (C₆),        2-amino-4-fluorophenyl (C₆), 2-(N,N-diethylamino)phenyl (C₆),        2-cyanophenyl (C₆), 2,6-di-tert-butylphenyl (C₆),        3-methoxyphenyl (C₆), 8-hydroxynaphthylen-2-yl (C₁₀),        4,5-dimethoxynaphthylen-1-yl (C₁₀), and 6-cyano-naphthylen-1-yl        (C₁₀).    -   ii) C₆ or C₁₀ aryl rings fused with 1 or 2 saturated rings to        afford C₈-C₂₀ ring systems, non-limiting examples of which        include, bicyclo[4.2.0]octa-1,3,5-trienyl (C₈), and indanyl        (C₉).

-   3) The terms “heterocyclic” and/or “heterocycle” are defined herein    as “units comprising one or more rings having from 3 to 20 atoms    wherein at least one atom in at least one ring is a heteroatom    chosen from nitrogen (N), oxygen (O), or sulfur (S), or mixtures of    N, O, and S, and wherein further the ring which contains the    heteroatom is also not an aromatic ring.” The following are    non-limiting examples of “substituted and unsubstituted heterocyclic    rings” which encompass the following categories of units:    -   i) heterocyclic units having a single ring containing one or        more heteroatoms, non-limiting examples of which include,        diazirinyl (C₁), aziridinyl (C₂), urazolyl (C₂), azetidinyl        (C₃), pyrazolidinyl (C₃), imidazolidinyl (C₃), oxazolidinyl        (C₃), isoxazolinyl (C₃), thiazolidinyl (C₃), isothiazolinyl        (C₃), oxathiazolidinonyl (C₃), oxazolidinonyl (C₃), hydantoinyl        (C₃), tetrahydrofuranyl (C₄), pyrrolidinyl (C₄), morpholinyl        (C₄), piperazinyl (C₄), piperidinyl (C₄), dihydropyranyl (C₅),        tetrahydropyranyl (C₅), piperidin-2-onyl (valerolactam) (C₅),        2,3,4,5-tetrahydro-1H-azepinyl (C₆), 2,3-dihydro-1H-indole (C₈),        and 1,2,3,4-tetrahydroquinoline (C₉).    -   ii) heterocyclic units having 2 or more rings one of which is a        heterocyclic ring, non-limiting examples of which include        hexahydro-1H-pyrrolizinyl (C₇),        3a,4,5,6,7,7a-hexahydro-1H-benzo[d]imidazolyl (C₇),        3a,4,5,6,7,7a-hexahydro-1H-indolyl (C₈),        1,2,3,4-tetrahydroquinolinyl (C₉), and        decahydro-1H-cycloocta[b]pyrrolyl (C₁₀).

-   4) The term “heteroaryl” is defined herein as “encompassing one or    more rings comprising from 5 to 20 atoms wherein at least one atom    in at least one ring is a heteroatom chosen from nitrogen (N),    oxygen (O), or sulfur (S), or mixtures of N, O, and S, and wherein    further at least one of the rings which comprises a heteroatom is an    aromatic ring.” The following are non-limiting examples of    “substituted and unsubstituted heterocyclic rings” which encompass    the following categories of units:    -   i) heteroaryl rings containing a single ring, non-limiting        examples of which include, 1,2,3,4-tetrazolyl (C₁),        [1,2,3]triazolyl (C₂), [1,2,4]triazolyl (C₂), triazinyl (C₃),        thiazolyl (C₃), 1H-imidazolyl (C₃), oxazolyl (C₃), isoxazolyl        (C₃), isothiazolyl (C₃), furanyl (C₄), thiophenyl (C₄),        pyrimidinyl (C₄), 2-phenylpyrimidinyl (C₄), pyridinyl (C₅),        3-methylpyridinyl (C₅), and 4-dimethylaminopyridinyl (C₅)    -   ii) heteroaryl rings containing 2 or more fused rings one of        which is a heteroaryl ring, non-limiting examples of which        include: 7H-purinyl (C₅), 9H-purinyl (C₅), 6-amino-9H-purinyl        (C₅), 5H-pyrrolo[3,2-d]pyrimidinyl (C₆),        7H-pyrrolo[2,3-d]pyrimidinyl (C₆), pyrido[2,3-d]pyrimidinyl        (C₇), 2-phenylbenzo[d]thiazolyl (C₇), 1H-indolyl (C₈),        4,5,6,7-tetrahydro-1-H-indolyl (C₈), quinoxalinyl (C₈),        5-methylquinoxalinyl (C₈), quinazolinyl (C₈), quinolinyl (C₉),        8-hydroxy-quinolinyl (C₉), and isoquinolinyl (C₉).

-   5) C₁-C₆ tethered cyclic hydrocarbyl units (whether carbocyclic    units, C₆ or C₁₀ aryl units, heterocyclic units, or heteroaryl    units) which connected to another moiety, unit, or core of the    molecule by way of a C₁-C₆ alkylene unit. Non-limiting examples of    tethered cyclic hydrocarbyl units include benzyl C₁-(C₆) having the    formula:

-   -   wherein R^(a) is optionally one or more independently chosen        substitutions for hydrogen. Further examples include other aryl        units, inter alia, (2-hydroxyphenyl)hexyl C₆-(C₆);        naphthalen-2-ylmethyl C₁-(C₁₀), 4-fluorobenzyl C₁-(C₆),        2-(3-hydroxyphenyl)ethyl C₂-(C₆), as well as substituted and        unsubstituted C₃-C₁₀ alkylenecarbocyclic units, for example,        cyclopropylmethyl C₁-(C₃), cyclopentylethyl C₂-(C₅),        cyclohexylmethyl C₁-(C₆). Included within this category are        substituted and unsubstituted C₁-C₁₀ alkylene-heteroaryl units,        for example a 2-picolyl C₁-(C₆) unit having the formula:

-   -   wherein R^(a) is the same as defined above. In addition, C₁-C₁₂        tethered cyclic hydrocarbyl units include C₁-C₁₀        alkyleneheterocyclic units and alkylene-heteroaryl units,        non-limiting examples of which include, aziridinylmethyl C₁-(C₂)        and oxazol-2-ylmethyl C₁-(C₃).

For the purposes of the present disclosure carbocyclic rings are from C₃to C₂₀; aryl rings are C₆ or C₁₀; heterocyclic rings are from C₁ to C₉;and heteroaryl rings are from C₁ to C₉.

For the purposes of the present disclosure, and to provide consistencyin defining the present disclosure, fused ring units, as well asspirocyclic rings, bicyclic rings and the like, which comprise a singleheteroatom will be characterized and referred to herein as beingencompassed by the cyclic family corresponding to the heteroatomcontaining ring, although the artisan may have alternativecharacterizations. For example, 1,2,3,4-tetrahydroquinoline having theformula:

is, for the purposes of the present disclosure, considered aheterocyclic unit. 6,7-Dihydro-5H-cyclopentapyrimidine having theformula:

is, for the purposes of the present disclosure, considered a heteroarylunit. When a fused ring unit contains heteroatoms in both a saturatedring (heterocyclic ring) and an aryl ring (heteroaryl ring), the arylring will predominate and determine the type of category to which thering is assigned herein for the purposes of describing the presentdisclosure. For example, 1,2,3,4-tetrahydro-[1,8]naphthpyridine havingthe formula:

is, for the purposes of the present disclosure, considered a heteroarylunit.

The term “substituted” is used throughout the specification. The term“substituted” is applied to the units described herein as “substitutedunit or moiety is a hydrocarbyl unit or moiety, whether acyclic orcyclic, which has one or more hydrogen atoms replaced by a substituentor several substituents as defined herein below.” The units, whensubstituting for hydrogen atoms are capable of replacing one hydrogenatom, two hydrogen atoms, or three hydrogen atoms of a hydrocarbylmoiety at a time. In addition, these substituents can replace twohydrogen atoms on two adjacent carbons to form said substituent, newmoiety, or unit. For example, a substituted unit that requires a singlehydrogen atom replacement includes halogen, hydroxyl, and the like. Atwo hydrogen atom replacement includes carbonyl, oximino, and the like.A two hydrogen atom replacement from adjacent carbon atoms includesepoxy, and the like. Three hydrogen replacement includes cyano, and thelike. The term substituted is used throughout the present specificationto indicate that a hydrocarbyl moiety, inter alia, aromatic ring, alkylchain; can have one or more of the hydrogen atoms replaced by asubstituent. When a moiety is described as “substituted” any number ofthe hydrogen atoms may be replaced. For example, 4-hydroxyphenyl is a“substituted aromatic carbocyclic ring (aryl ring)”,(N,N-dimethyl-5-amino)octanyl is a “substituted C₈ linear alkyl unit,3-guanidinopropyl is a “substituted C₃ linear alkyl unit,” and2-carboxypyridinyl is a “substituted heteroaryl unit.”

The following are non-limiting examples of units which can substitutefor hydrogen atoms on a carbocyclic, aryl, heterocyclic, or heteroarylunit:

-   -   i) C₁-C₁₂ linear, branched, or cyclic alkyl, alkenyl, and        alkynyl; methyl (C₁), ethyl (C₂), ethenyl (C₂), ethynyl (C₂),        n-propyl (C₃), iso-propyl (C₃), cyclopropyl (C₃), 3-propenyl        (C₃), 1-propenyl (also 2-methylethenyl) (C₃), isopropenyl (also        2-methylethen-2-yl) (C₃), prop-2-ynyl (also propargyl) (C₃),        propyn-1-yl (C₃), n-butyl (C₄), sec-butyl (C₄), iso-butyl (C₄),        tert-butyl (C₄), cyclobutyl (C₄), buten-4-yl (C₄), cyclopentyl        (C₅), cyclohexyl (C₆);    -   ii) substituted or unsubstituted C₆ or C₁₀ aryl; for example,        phenyl, naphthyl (also referred to herein as naphthylen-1-yl        (C₁₀) or naphthylen-2-yl (C₁₀));    -   iii) substituted or unsubstituted C₆ or C₁₀ alkylenearyl; for        example, benzyl, 2-phenylethyl, naphthylen-2-ylmethyl;    -   iv) substituted or unsubstituted C₁-C₉ heterocyclic rings; as        described herein below;    -   v) substituted or unsubstituted C₁-C₉ heteroaryl rings; as        described herein below;    -   vi) —(CR^(102a)R^(102b))_(a)OR¹⁰¹; for example, —OH, —CH₂OH,        —OCH₃, —CH₂OCH₃, —OCH₂CH₃, —CH₂OCH₂CH₃, —OCH₂CH₂CH₃, and        —CH₂OCH₂CH₂CH₃;    -   vii) —(CR^(102a)R^(102b))_(a)C(O)R¹⁰¹; for example, —COCH₃,        —CH₂COCH₃, —COCH₂CH₃, —CH₂COCH₂CH₃, —COCH₂CH₂CH₃, and        —CH₂COCH₂CH₂CH₃;    -   viii) for example, —CO₂CH₃, —CH₂CO₂CH₃, —CO₂CH₂CH₃,        —CH₂CO₂CH₂CH₃, —CO₂CH₂CH₂CH₃, and —CH₂CO₂CH₂CH₂CH₃;    -   ix) —(CR^(102a)R^(102b))_(a)C(O)N(R¹⁰¹)₂; for example, —CONH₂,        —CH₂CONH₂, —CONHCH₃, —CH₂CONHCH₃, —CON(CH₃)₂, and —CH₂CON(CH₃)₂;    -   x) —(CR^(102a)R^(102b))_(a)N(R¹⁰¹)₂; for example, —NH₂, —CH₂NH₂,        —NHCH₃, —CH₂NHCH₃, —N(CH₃)₂, and —CH₂N(CH₃)₂;    -   xi) halogen; —F, —Cl, —Br, and —I;    -   xii) —(CR^(102a)R^(102b))_(a)CN;    -   xiii) —(CR^(102a)R^(102b))_(a)NO₂;    -   xiv) —CH_(j)X_(k); wherein X is halogen, the index j is an        integer from 0 to 2, j+k=3; for example, —CH₂F, —CHF₂, —CF₃,        —CCl₃, or —CBr₃;    -   xv) —(CR^(102a)R^(102b))_(a)SR¹⁰¹; —SH, —CH₂SH, —SCH₃, —CH₂SCH₃,        —SC₆H₅, and —CH₂SC₆H₅;    -   xvi) —(CR^(102a)R^(102b))_(a)SO₂R¹⁰¹; for example, —SO₂H,        —CH₂SO₂H, —SO₂CH₃, —CH₂SO₂CH₃, —SO₂C₆H₅, and —CH₂SO₂C₆H₅; and    -   xvii) —(CR^(102a)R^(102b))_(a)SO₃R¹⁰¹; for example, —SO₃H,        —CH₂SO₃H, —SO₃CH₃, —CH₂SO₃CH₃, —SO₃C₆H₅, and —CH₂SO₃C₆H₅;        wherein each R¹⁰¹ is independently hydrogen, substituted or        unsubstituted C₁-C₆ linear, branched, or cyclic alkyl, phenyl,        benzyl, heterocyclic, or heteroaryl; or two R¹⁰¹ units can be        taken together to form a ring comprising 3-7 atoms; R^(102a) and        R^(102b) are each independently hydrogen or C₁-C₄ linear or        branched alkyl; the index “a” is from 0 to 4.

For the purposes of the present disclosure the terms “compound,”“analog,” and “composition of matter” stand equally well for each otherand are used interchangeably throughout the specification. The disclosedcompounds include all enantiomeric forms, diastereomeric forms, salts,and the like.

The compounds disclosed herein include all salt forms, for example,salts of both basic groups, inter alia, amines, as well as salts ofacidic groups, inter alia, carboxylic acids. The following arenon-limiting examples of anions that can form salts with protonatedbasic groups: chloride, bromide, iodide, sulfate, bisulfate, carbonate,bicarbonate, phosphate, formate, acetate, propionate, butyrate,pyruvate, lactate, oxalate, malonate, maleate, succinate, tartrate,fumarate, citrate, and the like. The following are non-limiting examplesof cations that can form salts of acidic groups: ammonium, sodium,lithium, potassium, calcium, magnesium, bismuth, lysine, and the like.

The disclosed compounds have Formula (I):

wherein the carbon atom having the amino unit has the (S)stereochemistry as indicated in the following formula:

The units which comprise R and Z can comprise units having anyconfiguration, and, as such, the disclosed compounds can be singleenantiomers, diastereomeric pairs, or combinations thereof. In addition,the compounds can be isolated as salts or hydrates. In the case ofsalts, the compounds can comprises more than one cation or anion. In thecase of hydrates, any number of water molecules, or fractional partthereof (for example, less than 1 water molecule present for eachmolecule of analog) can be present.

R Units

R is a substituted or unsubstituted thiazolyl unit having the formula:

R², R³, and R⁴ are substituent groups that can be independently chosenfrom a wide variety of non-carbon atom containing units (for example,hydrogen, hydroxyl, amino, halogen, nitro, and the like) or organicsubstituent units, such as substituted and unsubstituted acyclichydrocarbyl and cyclic hydrocarbyl units as described herein. The carboncomprising units can comprise from 1 to 12 carbon atoms, or 1 to 10carbon atoms, or 1 to 6 carbon atoms.

An example of compounds of Formula (I) include compounds wherein R unitsare thiazol-2-yl units having the formula:

wherein R² and R³ are each independently chosen from:

-   -   i) hydrogen;    -   ii) substituted or unsubstituted C₁-C₆ linear, branched, or        cyclic alkyl;    -   iii) substituted or unsubstituted C₂-C₆ linear, branched, or        cyclic alkenyl;    -   iv) substituted or unsubstituted C₂-C₆ linear or branched        alkynyl;    -   v) substituted or unsubstituted C₆ or C₁₀ aryl;    -   vi) substituted or unsubstituted C₁-C₉ heteroaryl;    -   vii) substituted or unsubstituted C₁-C₉ heterocyclic; or    -   viii) R² and R³ can be taken together to form a saturated or        unsaturated ring having from 5 to 7 atoms; wherein from 1 to 3        atoms can optionally be heteroatoms chosen from oxygen,        nitrogen, and sulfur.

The following are non-limiting examples of units that can substitute forone or more hydrogen atoms on the R² and R³ units. The followingsubstituents, as well as others not herein described, are eachindependently chosen:

-   -   i) C₁-C₁₂ linear, branched, or cyclic alkyl, alkenyl, and        alkynyl; methyl (C₁), ethyl (C₂), ethenyl (C₂), ethynyl (C₂),        n-propyl (C₃), iso-propyl (C₃), cyclopropyl (C₃), 3-propenyl        (C₃), 1-propenyl (also 2-methylethenyl) (C₃), isopropenyl (also        2-methylethen-2-yl) (C₃), prop-2-ynyl (also propargyl) (C₃),        propyn-1-yl (C₃), n-butyl (C₄), sec-butyl (C₄), iso-butyl (C₄),        tert-butyl (C₄), cyclobutyl (C₄), buten-4-yl (C₄), cyclopentyl        (C₅), cyclohexyl (C₆);    -   ii) substituted or unsubstituted C₆ or C₁₀ aryl; for example,        phenyl, naphthyl (also referred to herein as naphthylen-1-yl        (C₁₀) or naphthylen-2-yl (C₁₀);    -   iii) substituted or unsubstituted C₆ or C₁₀ alkylenearyl; for        example, benzyl, 2-phenylethyl, naphthylen-2-ylmethyl;    -   iv) substituted or unsubstituted C₁-C₉ heterocyclic rings; as        described herein;    -   v) substituted or unsubstituted C₁-C₉ heteroaryl rings; as        described herein;    -   vi) —(CR^(21a)R^(21b))_(p)OR²⁰; for example, —OH, —CH₂OH, —OCH₃,        —CH₂OCH₃, OCH₂CH₃, —CH₂OCH₂CH₃, —OCH₂CH₂CH₃, and —CH₂OCH₂CH₂CH₃;    -   vii) —(CR^(21a)R^(21b))_(p)C(O)R²⁰; for example, —COCH₃,        —CH₂COCH₃, —COCH₂CH₃, —CH₂COCH₂CH₃, —COCH₂CH₂CH₃, and        —CH₂COCH₂CH₂CH₃;    -   viii) —(CR^(21a)R^(21b))_(p)C(O)OR²⁰; for example, —CO₂CH₃,        —CH₂CO₂CH₃, —CO₂CH₂CH₃, —CH₂CO₂CH₂CH₃, —CO₂CH₂CH₂CH₃, and        —CH₂CO₂CH₂CH₂CH₃;    -   x) —(CR^(21a)R^(21b))_(p)C(O)N(R²⁰)₂; for example, —CONH₂,        —CH₂CONH₂, —CONHCH₃, —CH₂CONHCH₃, —CON(CH₃)₂, and —CH₂CON(CH₃)₂;    -   x) (CR^(21a)R^(21b))_(p)N(R²⁰)₂; for example, —NH₂, —CH₂NH₂,        —NHCH₃, —CH₂NHCH₃, —N(CH₃)₂, and —CH₂N(CH₃)₂;    -   xi) halogen; —F, —Cl, —Br, and —I;    -   xii) —(CR^(21a)R^(21b))_(p)CN;    -   xiii) —(CR^(21a)R^(21b))_(p)NO₂;    -   xiv) —(CH_(j′)X_(k′))_(h)CH_(j)X_(k); wherein X is halogen, the        index j is an integer from 0 to 2, j+k=3, the index j′ is an        integer from 0 to 2, j′+k′=2, the index h is from 0 to 6; for        example, —CH₂F, —CHF₂, —CF₃, —CH₂CF₃, —CHFCF₃, —CCl₃, or —CBr₃;    -   xv) —(CR^(21a)R^(21b))_(p)SR²⁰; —SH, —CH₂SH, —SCH₃, —CH₂SCH₃,        —SC₆H₅, and CH₂SC₆H₅;    -   xvi) —(CR^(21a)R^(21b))_(p)SO₂R²⁰; for example, —SO₂H, —CH₂SO₂H,        —SO₂CH₃, —CH₂SO₂CH₃, —SO₂C₆H₅, and —CH₂SO₂C₆H₅; and    -   xvii) —(CR^(21a)R^(21b))_(p)SO₃R²⁰; for example, —SO₃H,        —CH₂SO₃H, —SO₃CH₃, CH₂SO₃CH₃, —SO₃C₆H₅, and —CH₂SO₃C₆H₅;        wherein each R²⁰ is independently hydrogen, substituted or        unsubstituted C₁-C₄ linear, branched, or cyclic alkyl, phenyl,        benzyl, heterocyclic, or heteroaryl; or two R²⁰ units can be        taken together to form a ring comprising 3-7 atoms; R^(21a) and        R^(21b) are each independently hydrogen or C₁-C₄ linear or        branched alkyl; the index p is from 0 to 4.

An example of compounds of Formula (I) includes R units having theformula:

wherein R³ is hydrogen and R² is a unit chosen from methyl (C₁), ethyl(C₂), n-propyl (C₃), iso-propyl (C₃), n-butyl (C₄), sec-butyl (C₄),iso-butyl (C₄), tert-butyl (C₄), n-pentyl (C₅), 1-methylbutyl (C₅),2-methylbutyl (C₅), 3-methylbutyl (C₅), cyclopropyl (C₃), n-hexyl (C₆),4-methylpentyl (C₆), and cyclohexyl (C₆).

Another example of compounds of Formula (I) include R units having theformula:

wherein R² is a unit chosen from methyl (C₁), ethyl (C₂), n-propyl (C₃),iso-propyl (C₃), n-butyl (C₄), sec-butyl (C₄), iso-butyl (C₄), andtert-butyl (C₄); and R³ is a unit chosen from methyl (C₁) or ethyl (C₂).Non-limiting examples of this aspect of R includes4,5-dimethylthiazol-2-yl, 4-ethyl-5-methylthiazol-2-yl,4-methyl-5-ethylthiazol-2-yl, and 4,5-diethylthiazol-2-yl.

A further example of compounds of Formula (I) includes R units whereinR³ is hydrogen and R² is a substituted alkyl unit, said substitutionschosen from:

i) halogen: —F, —Cl, —Br, and —I;

ii) —N(R¹¹)₂; and

iii) —OR¹¹;

wherein each R¹¹ is independently hydrogen or C₁-C₄ linear or branchedalkyl. Non-limiting examples of units that can be a substitute for a R²or R³ hydrogen atom on R units include CH₂F, —CHF₂, —CF₃, —CH₂CF₃,—CH₂CH₂CF₃, —CH₂Cl, —CH₂OH, —CH₂OCH₃, —CH₂CH₂OH, —CH₂CH₂OCH₃, —CH₂NH₂,—CH₂NHCH₃, —CH₂N(CH₃)₂, and —CH₂NH(CH₂CH₃).

Further non-limiting examples of units that can be a substitute for a R²or R³ hydrogen atom on R units include 2,2-difluorocyclopropyl,2-methoxycyclohexyl, and 4-chlorocyclohexyl.

A yet further example of compounds of Formula (I), R units include unitswherein R³ is hydrogen and R² is phenyl or substituted phenyl, whereinnon-limiting examples of R² units include phenyl, 3,4-dimethylphenyl,4-tert-butylphenyl, 4-cyclopropylphenyl, 4-diethylaminophenyl,4-(trifluoromethyl)phenyl, 4-methoxyphenyl, 4-(difluoromethoxy)phenyl,4-(trifluoromethoxy)phenyl, 3-chloropheny, 4-chlorophenyl, and3,4-dichlorophenyl, which when incorporated into the definition of Raffords the following R units 4-phenylthiazol-2-yl,3,4-dimethylphenylthiazol-2-yl, 4-tert-butylphenylthiazol-2-yl,4-cyclopropylphenylthiazol-2-yl, 4-diethylaminophenylthiazol-2-yl,4-(trifluoromethyl)phenylthiazol-2-yl, 4-methoxyphenylthiazol-2-yl,4-(difluoromethoxy)phenylthiazol-2-yl,4-(trifluoromethoxy)phenylthiazol-2-yl, 3-chlorophenylthiazol-2-yl,4-chlorophenylthiazol-2-yl, and 3,4-dichlorophenylthiazol-2-yl.

A still further example of compounds of Formula (I) includes R unitswherein R² is chosen from hydrogen, methyl, ethyl, n-propyl, andiso-propyl and R³ is phenyl or substituted phenyl. A non-limitingexample of a R unit according to the fifth aspect of the first categoryof R units includes 4-methyl-5-phenylthiazol-2-yl and4-ethyl-5-phenylthiazol-2-yl.

Another further example of compounds of Formula (I) includes R unitswherein R³ is hydrogen and R² is a substituted or unsubstitutedheteroaryl unit chosen from 1,2,3,4-tetrazol-1-yl,1,2,3,4-tetrazol-5-yl, [1,2,3]triazol-4-yl, [1,2,3]triazol-5-yl,[1,2,4]triazol-4-yl, [1,2,4]triazol-5-yl, imidazol-2-yl, imidazol-4-yl,pyrrol-2-yl, pyrrol-3-yl, oxazol-2-yl, oxazol-4-yl, oxazol-5-yl,isoxazol-3-yl, isoxazol-4-yl, isoxazol-5-yl, [1,2,4]oxadiazol-3-yl,[1,2,4]oxadiazol-5-yl, [1,3,4]oxadiazol-2-yl, furan-2-yl, furan-3-yl,thiophen-2-yl, thiophen-3-yl, isothiazol-3-yl, isothiazol-4-yl,isothiazol-5-yl, thiazol-2-yl, thiazol-4-yl, thiazol-5-yl,[1,2,4]thiadiazol-3-yl, [1,2,4]thiadiazol-5-yl, and[1,3,4]thiadiazol-2-yl.

Further non-limiting example of compounds of Formula (I) includes Runits wherein R² is substituted or unsubstituted thiophen-2-yl, forexample thiophen-2-yl, 5-chlorothiophen-2-yl, and 5-methylthiophen-2-yl.

A still further example of compounds of Formula (I) includes R unitswherein R² is substituted or unsubstituted thiophen-3-yl, for examplethiophen-3-yl, 5-chlorothiophen-3-yl, and 5-methylthiophen-3-yl.

Another example of compounds of Formula (I) includes R units wherein R²and R³ are taken together to form a saturated or unsaturated ring havingfrom 5 to 7 atoms. Non-limiting examples of the sixth aspect of thefirst category of R units include5,6-dihydro-4H-cyclopenta[d]thiazol-2-yl and4,5,6,7-tetrahydrobenzo[d]thiazol-2-yl.

Further examples of compounds of Formula (I) include R units that arethiazol-4-yl units having the formula:

wherein R⁴ is a unit chosen from:

i) hydrogen;

ii) substituted or unsubstituted C₁-C₆ linear, branched, or cyclicalkyl;

iii) substituted or unsubstituted C₂-C₆ linear, branched, or cyclicalkenyl;

iv) substituted or unsubstituted C₂-C₆ linear or branched alkynyl;

v) substituted or unsubstituted C₆ or C₁₀ aryl;

vi) substituted or unsubstituted C₁-C₉ heteroaryl; or

vii) substituted or unsubstituted C₁-C₉ heterocyclic.

The following are non-limiting examples of units that can substitute forone or more hydrogen atoms on the R⁴ units. The following substituents,as well as others not herein described, are each independently chosen:

-   -   i) C₁-C₁₂ linear, branched, or cyclic alkyl, alkenyl, and        alkynyl; methyl (C₁), ethyl (C₂), ethenyl (C₂), ethynyl (C₂),        n-propyl (C₃), iso-propyl (C₃), cyclopropyl (C₃), 3-propenyl        (C₃), 1-propenyl (also 2-methylethenyl) (C₃), isopropenyl (also        2-methylethen-2-yl) (C₃), prop-2-ynyl (also propargyl) (C₃),        propyn-1-yl (C₃), n-butyl (C₄), sec-butyl (C₄), iso-butyl (C₄),        tert-butyl (C₄), cyclobutyl (C₄), buten-4-yl (C₄), cyclopentyl        (C₅), cyclohexyl (C₆);    -   ii) substituted or unsubstituted C₆ or C₁₀ aryl; for example,        phenyl, naphthyl (also referred to herein as naphthylen-1-yl        (C₁₀) or naphthylen-2-yl (C₁₀));    -   iii) substituted or unsubstituted C₆ or C₁₀ alkylenearyl; for        example, benzyl, 2-phenylethyl, naphthylen-2-ylmethyl;    -   iv) substituted or unsubstituted C₁-C₉ heterocyclic rings; as        described herein below;    -   v) substituted or unsubstituted C₁-C₉ heteroaryl rings; as        described herein below;    -   vi) —(CR^(21a)R^(21b))_(p)OR²⁰; for example, —OH, —CH₂OH, —OCH₃,        —CH₂OCH₃, OCH₂CH₃, —CH₂OCH₂CH₃, —OCH₂CH₂CH₃, and —CH₂OCH₂CH₂CH₃;    -   vii) —(CR^(21a)R_(21b))_(p)C(O)R²⁰; for example, —COCH₃,        —CH₂COCH₃, —COCH₂CH₃, —CH₂COCH₂CH₃, —COCH₂CH₂CH₃, and        —CH₂COCH₂CH₂CH₃;    -   viii) —(CR^(21a)R_(21b))_(p)C(O)OR²⁰; for example, —CO₂CH₃,        —CH₂CO₂CH₃, —CO₂CH₂CH₃, —CH₂CO₂CH₂CH₃, —CO₂CH₂CH₂CH₃, and        —CH₂CO₂CH₂CH₂CH₃;    -   xi) —(CR^(21a)R_(21b))_(p)C(O)N(R²⁰)₂; for example, —CONH₂,        —CH₂CONH₂, —CONHCH₃, —CH₂CONHCH₃, —CON(CH₃)₂, and —CH₂CON(CH₃)₂;    -   x) —(CR^(21a)R_(21b))_(p)N(R²⁰)₂; for example, —NH₂, —CH₂NH₂,        —NHCH₃, —CH₂NHCH₃, —N(CH₃)₂, and —CH₂N(CH₃)₂;    -   xi) halogen; —F, —Cl, —Br, and —I;    -   xii) —(CR^(21a)R_(21b))_(p)CN;    -   xiii) —(CR^(21a)R_(21b))_(p)NO₂;    -   xiv) —(CH_(j′)X_(k′))_(h)CH_(j)X_(k); wherein X is halogen, the        index j is an integer from 0 to 2, j+k=3, the index j′ is an        integer from 0 to 2, j′+k′=2, the index h is from 0 to 6; for        example, —CH₂F, —CHF₂, —CF₃, —CH₂CF₃, —CHFCF₃, —CCl₃, or —CBr₃;    -   xv) —(CR^(21a)R_(21b))_(p)SR²⁰; —SH, —CH₂SH, —SCH₃, —CH₂SCH₃,        —SC₆H₅, and —CH₂SC₆H₅;    -   xvi) —(CR^(21a)R_(21b))_(p)SO₂R²⁰; for example, —SO₂H, —CH₂SO₂H,        —SO₂CH₃, —CH₂SO₂CH₃, —SO₂C₆H₅, and —CH₂SO₂C₆H₅; and    -   xvii) —(CR^(21a)R_(21b))_(p)SO₃R²⁰; for example, —SO₃H,        —CH₂SO₃H, —SO₃CH₃, —CH₂SO₃CH₃, —SO₃C₆H₅, and —CH₂SO₃C₆H₅;        wherein each R²⁰ is independently hydrogen, substituted or        unsubstituted C₁-C₄ linear, branched, or cyclic alkyl, phenyl,        benzyl, heterocyclic, or heteroaryl; or two R²⁰ units can be        taken together to form a ring comprising 3-7 atoms; R^(21a) and        R^(21b) are each independently hydrogen or C₁-C₄ linear or        branched alkyl; the index p is from 0 to 4.

An example of compounds of Formula (I) includes R units wherein R⁴ ishydrogen.

A further example of compounds of Formula (I) includes R units whereinR⁴ is a unit chosen from methyl (C₁), ethyl (C₂), n-propyl (C₃),iso-propyl (C₃), n-butyl (C₄), sec-butyl (C₄), iso-butyl (C₄), andtert-butyl (C₄). Non-limiting examples of this aspect of R includes2-methylthiazol-4-yl, 2-ethylthiazol-4-yl, 2-(n-propyl)thiazol-4-yl, and2-(iso-propyl)thiazol-4-yl.

A still further example of compounds of Formula (I) includes R unitswherein R⁴ is substituted or unsubstituted phenyl, non-limiting examplesof which include phenyl, 2-fluorophenyl, 2-chlorophenyl, 2-methylphenyl,2-methoxyphenyl, 3-fluorophenyl, 3-chlorophenyl, 3-methylphenyl,3-methoxyphenyl, 4-fluorophenyl, 4-chlorophenyl, 4-methylphenyl, and4-methoxyphenyl.

Yet further example of compounds of Formula (I) includes R units whereinR⁴ is substituted or unsubstituted heteroaryl, non-limiting examples ofwhich include thiophen-2-yl, thiophen-3-yl, thiazol-2-yl, thiazol-4-yl,thiazol-5-yl, 2,5-dimethylthiazol-4-yl, 2,4-dimethylthiazol-5-yl,4-ethylthiazol-2-yl, oxazol-2-yl, oxazol-4-yl, oxazol-5-yl, and3-methyl-1,2,4-oxadiazol-5-yl.

Another example of 5-member ring R units includes substituted orunsubstituted imidazolyl units having the formula:

One example of imidazolyl R units includes imidazol-2-yl units havingthe formula:

wherein R² and R³ are each independently chosen from:

-   -   i) hydrogen;    -   ii) substituted or unsubstituted C₁-C₆ linear, branched, or        cyclic alkyl;    -   iii) substituted or unsubstituted C₂-C₆ linear, branched, or        cyclic alkenyl;    -   iv) substituted or unsubstituted C₂-C₆ linear or branched        alkynyl;    -   v) substituted or unsubstituted C₆ or C₁₀ aryl;    -   vi) substituted or unsubstituted C₁-C₉ heteroaryl;    -   vii) substituted or unsubstituted C₁-C₉ heterocyclic; or    -   viii) R² and R³ can be taken together to form a saturated or        unsaturated ring having from 5 to 7 atoms; wherein from 1 to 3        atoms can optionally be heteroatoms chosen from oxygen,        nitrogen, and sulfur.

The following are non-limiting examples of units that can substitute forone or more hydrogen atoms on the R² and R³ units. The followingsubstituents, as well as others not herein described, are eachindependently chosen:

-   -   i) C₁-C₁₂ linear, branched, or cyclic alkyl, alkenyl, and        alkynyl; methyl (C₁), ethyl (C₂), ethenyl (C₂), ethynyl (C₂),        n-propyl (C₃), iso-propyl (C₃), cyclopropyl (C₃), 3-propenyl        (C₃), 1-propenyl (also 2-methylethenyl) (C₃), isopropenyl (also        2-methylethen-2-yl) (C₃), prop-2-ynyl (also propargyl) (C₃),        propyn-1-yl (C₃), n-butyl (C₄), sec-butyl (C₄), iso-butyl (C₄),        tert-butyl (C₄), cyclobutyl (C₄), buten-4-yl (C₄), cyclopentyl        (C₅), cyclohexyl (C₆);    -   ii) substituted or unsubstituted C₆ or C₁₀ aryl; for example,        phenyl, naphthyl (also referred to herein as naphthylen-1-yl        (C₁₀) or naphthylen-2-yl (C₁₀));    -   iii) substituted or unsubstituted C₆ or C₁₀ alkylenearyl; for        example, benzyl, 2-phenylethyl, naphthylen-2-ylmethyl;    -   iv) substituted or unsubstituted C₁-C₉ heterocyclic rings; as        described herein;    -   v) substituted or unsubstituted C₁-C₉ heteroaryl rings; as        described herein;    -   vi) —(CR^(21a)R^(21b))_(z)OR²⁰; for example, —OH, —CH₂OH, —OCH₃,        —CH₂OCH₃, —OCH₂CH₃, —CH₂OCH₂CH₃, —OCH₂CH₂CH₃, and        —CH₂OCH₂CH₂CH₃;    -   vii) —(CR^(21a)R^(21b))_(z)C(O)R²⁰; for example, —COCH₃,        —CH₂COCH₃, —COCH₂CH₃, —CH₂COCH₂CH₃, —COCH₂CH₂CH₃, and        —CH₂COCH₂CH₂CH₃;    -   viii) —(CR^(21a)R^(21b))_(z)C(O)OR²⁰; for example, —CO₂CH₃,        —CH₂CO₂CH₃, —CO₂CH₂CH₃, —CH₂CO₂CH₂CH₃, —CO₂CH₂CH₂CH₃, and        —CH₂CO₂CH₂CH₂CH₃;    -   xii) —(CR^(21a)R^(21b))_(z)C(O)N(R²⁰)₂; for example, —CONH₂,        —CH₂CONH₂, —CONHCH₃, —CH₂CONHCH₃, —CON(CH₃)₂, and —CH₂CON(CH₃)₂;    -   x) —(CR^(21a)R^(21b))_(z)N(R²⁰)₂; for example, —NH₂, —CH₂NH₂,        —NHCH₃, —CH₂NHCH₃, —N(CH₃)₂, and —CH₂N(CH₃)₂;    -   xi) halogen; —F, —Cl, —Br, and —I;    -   xii) —(CR^(21a)R^(21b))_(z)CN;    -   xiii) —(CR^(21a)R^(21b))_(z)NO₂;    -   xiv) —(CH_(j′)X_(k′))_(h)CH_(j)X_(k); wherein X is halogen, the        index j is an integer from 0 to 2, j+k=3, the index j′ is an        integer from 0 to 2, j′+k′=2, the index h is from 0 to 6; for        example, —CH₂F, —CHF₂, —CF₃, —CH₂CF₃, —CHFCF₃, —CCl₃, or —CBr₃;    -   xv) —(CR^(21a)R^(21b))_(z)SR²⁰; —SH, —CH₂SH, —SCH₃, —CH₂SCH₃,        —SC₆H₅, and CH₂SC₆H₅;    -   xvi) —(CR^(21a)R^(21b))_(z)SO₂R²⁰; for example, —SO₂H, —CH₂SO₂H,        —SO₂CH₃, —CH₂SO₂CH₃, —SO₂C₆H₅, and —CH₂SO₂C₆H₅; and    -   xvii) —(CR^(21a)R^(21b))_(z)SO₃R²⁰; for example, —SO₃H,        —CH₂SO₃H, —SO₃CH₃, —CH₂SO₃CH₃, —SO₃C₆H₅, and —CH₂SO₃C₆H₅;        wherein each R²⁰ is independently hydrogen, substituted or        unsubstituted C₁-C₄ linear, branched, or cyclic alkyl, phenyl,        benzyl, heterocyclic, or heteroaryl; or two R²⁰ units can be        taken together to form a ring comprising 3-7 atoms; R^(21a) and        R^(21b) are each independently hydrogen or C₁-C₄ linear or        branched alkyl; the index p is from 0 to 4.

One example of R units includes compounds wherein R units have theformula:

wherein R³ is hydrogen and R² is a unit chosen from methyl (C₁), ethyl(C₂), n-propyl (C₃), iso-propyl (C₃), n-butyl (C₄), sec-butyl (C₄),iso-butyl (C₄), and tert-butyl (C₄).

Another example of R units includes compounds wherein R² is a unitchosen from methyl (C₁), ethyl (C₂), n-propyl (C₃), iso-propyl (C₃),n-butyl (C₄), sec-butyl (C₄), iso-butyl (C₄), and tert-butyl (C₄); andR³ is a unit chosen from methyl (C₁) or ethyl (C₂). Non-limitingexamples of this aspect of R includes 4,5-dimethylimidazol-2-yl,4-ethyl-5-methylimidazol-2-yl, 4-methyl-5-ethylimidazol-2-yl, and4,5-diethylimidazol-2-yl.

An example of R units includes compounds wherein R³ is hydrogen and R²is a substituted alkyl unit chosen, said substitutions chosen from:

i) halogen: —F, —Cl, —Br, and —I;

ii) —N(R¹¹)₂; and

iii) —OR¹¹;

wherein each R¹¹ is independently hydrogen or C₁-C₄ linear or branchedalkyl.

Non-limiting examples of units comprising this embodiment of R includes:—CH₂F, —CHF₂, —CF₃, —CH₂CF₃, —CH₂Cl, —CH₂OH, —CH₂OCH₃, —CH₂CH₂OH,—CH₂CH₂OCH₃, —CH₂NH₂, —CH₂NHCH₃, —CH₂N(CH₃)₂, and —CH₂NH(CH₂CH₃).

A yet further example of R units include units wherein R³ is hydrogenand R² is phenyl.

A still further example of R units include units wherein R³ is hydrogenand R² is a heteroaryl unit chosen from 1,2,3,4-tetrazol-1-yl,1,2,3,4-tetrazol-5-yl, [1,2,3]triazol-4-yl, [1,2,3]triazol-5-yl,[1,2,4]triazol-4-yl, [1,2,4]triazol-5-yl, imidazol-2-yl, imidazol-4-yl,pyrrol-2-yl, pyrrol-3-yl, oxazol-2-yl, oxazol-4-yl, oxazol-5-yl,isoxazol-3-yl, isoxazol-4-yl, isoxazol-5-yl, [1,2,4]oxadiazol-3-yl,[1,2,4]oxadiazol-5-yl, [1,3,4]oxadiazol-2-yl, furan-2-yl, furan-3-yl,thiophen-2-yl, thiophen-3-yl, isothiazol-3-yl, isothiazol-4-yl,isothiazol-5-yl, thiazol-2-yl, thiazol-4-yl, thiazol-5-yl,[1,2,4]thiadiazol-3-yl, [1,2,4]thiadiazol-5-yl, and[1,3,4]thiadiazol-2-yl.

Z Units

Z is a unit having the formula:

-(L)_(n)-R¹

R¹ is chosen from:

i) hydrogen;

ii) hydroxyl;

iii) amino;

iv) substituted or unsubstituted C₁-C₆ linear, branched or cyclic alkyl;

v) substituted or unsubstituted C₁-C₆ linear, branched or cyclic alkoxy;

vi) substituted or unsubstituted C₆ or C₁₀ aryl;

vii) substituted or unsubstituted C₁-C₉ heterocyclic rings; or

viii) substituted or unsubstituted C₁-C₉ heteroaryl rings.

The following are non-limiting examples of units that can substitute forone or more hydrogen atoms on the R¹ units. The following substituents,as well as others not herein described, are each independently chosen:

-   -   i) C₁-C₁₂ linear, branched, or cyclic alkyl, alkenyl, and        alkynyl; methyl (C₁), ethyl (C₂), ethenyl (C₂), ethynyl (C₂),        n-propyl (C₃), iso-propyl (C₃), cyclopropyl (C₃), 3-propenyl        (C₃), 1-propenyl (also 2-methylethenyl) (C₃), isopropenyl (also        2-methylethen-2-yl) (C₃), prop-2-ynyl (also propargyl) (C₃),        propyn-1-yl (C₃), n-butyl (C₄), sec-butyl (C₄), iso-butyl (C₄),        tert-butyl (C₄), cyclobutyl (C₄), buten-4-yl (C₄), cyclopentyl        (C₅), cyclohexyl (C₆);    -   ii) substituted or unsubstituted C₆ or C₁₀ aryl; for example,        phenyl, naphthyl (also referred to herein as naphthylen-1-yl        (C₁₀) or naphthylen-2-yl (C₁₀));    -   iii) substituted or unsubstituted C₆ or C₁₀ alkylenearyl; for        example, benzyl, 2-phenylethyl, naphthylen-2-ylmethyl;    -   iv) substituted or unsubstituted C₁-C₉ heterocyclic rings; as        described herein;    -   v) substituted or unsubstituted C₁-C₉ heteroaryl rings; as        described herein;    -   vi) —(CR^(31a)R^(31b))_(q)OR³⁰; for example, —OH, —CH₂OH, —OCH₃,        —CH₂OCH₃, —OCH₂CH₃, —CH₂OCH₂CH₃, —OCH₂CH₂CH₃, and        —CH₂OCH₂CH₂CH₃;    -   vii) —(CR^(31a)R^(31b))_(q)C(O)R³⁰; for example, —COCH₃,        —CH₂COCH₃, —COCH₂CH₃, —CH₂COCH₂CH₃, —COCH₂CH₂CH₃, and        —CH₂COCH₂CH₂CH₃;    -   viii) —(CR^(31a)R^(31b))_(q)C(O)OR⁺; for example, —CO₂CH₃,        —CH₂CO₂CH₃, —CO₂CH₂CH₃, —CH₂CO₂CH₂CH₃, —CO₂CH₂CH₂CH₃, and        —CH₂CO₂CH₂CH₂CH₃;    -   xiii) —(CR^(31a)R^(31b))_(q)C(O)N(R³⁰)₂; for example, —CONH₂,        —CH₂CONH₂, —CONHCH₃, —CH₂CONHCH₃, —CON(CH₃)₂, and —CH₂CON(CH₃)₂;    -   x) —(CR^(31a)R^(31b))_(q)N(R³⁰)₂; for example, —NH₂, —CH₂NH₂,        —NHCH₃, —CH₂NHCH₃, —N(CH₃)₂, and —CH₂N(CH₃)₂;    -   xi) halogen; —F, —Cl, —Br, and —I;    -   xii) —(CR^(31a)R^(31b))_(q)CN;    -   xiii) —(CR^(31a)R^(31b))_(q)NO₂;    -   xiv) —(CH_(j′)X_(k′))_(h)CH_(j)X_(k); wherein X is halogen, the        index j is an integer from 0 to 2, j+k=3, the index j′ is an        integer from 0 to 2, j′+k′=2, the index h is from 0 to 6; for        example, —CH₂F, —CHF₂, —CF₃, —CH₂CF₃, —CHFCF₃, —CCl₃, or —CBr₃;    -   xv) —(CR^(31a)R^(31b))_(q)SR³⁰; —SH, —CH₂SH, —SCH₃, —CH₂SCH₃,        —SC₆H₅, and —CH₂SC₆H₅;    -   xvi) —(CR^(31a)R^(31b))_(q)SO₂R³⁰; for example, —SO₂H, —CH₂SO₂H,        —SO₂CH₃, —CH₂SO₂CH₃, —SO₂C₆H₅, and —CH₂SO₂C₆H₅; and    -   xvii) —(CR^(31a)R^(31b))_(q)SO₃R³⁰; for example, —SO₃H,        —CH₂SO₃H, —SO₃CH₃, —CH₂SO₃CH₃, —SO₃C₆H₅, and —CH₂SO₃C₆H₅;        wherein each R³⁰ is independently hydrogen, substituted or        unsubstituted C₁-C₆ linear, branched, or cyclic alkyl, phenyl,        benzyl, heterocyclic, or heteroaryl; or two R³⁰ units can be        taken together to form a ring comprising 3-7 atoms; R^(31a) and        R^(31b) are each independently hydrogen or C₁-C₄ linear or        branched alkyl; the index q is from 0 to 4.

One example of R¹ units includes substituted or unsubstituted phenyl (C₆aryl) units, wherein each substitution is independently chosen from:halogen, C₁-C₄ linear, branched alkyl, or cyclic alkyl, —OR¹¹, —CN,—N(R¹¹)₂, —CO₂R¹¹, —C(O)N(R¹¹)₂, —NR¹¹C(O)R¹¹, —NO₂, and —SO₂R¹¹; eachR¹¹ is independently hydrogen; substituted or unsubstituted C₁-C₄linear, branched, cyclic alkyl, alkenyl, or alkynyl; substituted orunsubstituted phenyl or benzyl; or two R¹¹ units can be taken togetherto form a ring comprising from 3-7 atoms.

Another example of R¹ units includes substituted C₆ aryl units chosenfrom phenyl, 2-fluorophenyl, 3-fluorophenyl, 4-fluorophenyl,2,3-difluorophenyl, 3,4-difluorophenyl, 3,5-difluorophenyl,2-chlorophenyl, 3-chlorophenyl, 4-chlorophenyl, 2,3-dichlorophenyl,3,4-dichlorophenyl, 3,5-dichlorophenyl, 2-hydroxyphenyl,3-hydroxyphenyl, 4-hydroxyphenyl, 2-methoxyphenyl, 3-methoxyphenyl,4-methoxyphenyl, 2,3-dimethoxyphenyl, 3,4-dimethoxyphenyl, and3,5-dimethoxyphenyl.

A further example of R¹ units includes substituted or unsubstituted C₆aryl units chosen from 2,4-difluorophenyl, 2,5-difluorophenyl,2,6-difluorophenyl, 2,3,4-trifluorophenyl, 2,3,5-trifluorophenyl,2,3,6-trifluorophenyl, 2,4,5-trifluorophenyl, 2,4,6-trifluorophenyl,2,4-dichlorophenyl, 2,5-dichlorophenyl, 2,6-dichlorophenyl,3,4-dichlorophenyl, 2,3,4-trichlorophenyl, 2,3,5-trichlorophenyl,2,3,6-trichlorophenyl, 2,4,5-trichlorophenyl, 3,4,5-trichlorophenyl, and2,4,6-trichlorophenyl.

A yet further example of R¹ units includes substituted C₆ aryl unitschosen from 2-methylphenyl, 3-methylphenyl, 4-methylphenyl,2,3-dimethylphenyl, 2,4-dimethylphenyl, 2,5-dimethylphenyl,2,6-dimethylphenyl, 3,4-dimethylphenyl, 2,3,4-trimethylphenyl,2,3,5-trimethylphenyl, 2,3,6-trimethylphenyl, 2,4,5-trimethylphenyl,2,4,6-trimethylphenyl, 2-ethylphenyl, 3-ethylphenyl, 4-ethylphenyl,2,3-diethylphenyl, 2,4-diethylphenyl, 2,5-diethylphenyl,2,6-diethylphenyl, 3,4-diethylphenyl, 2,3,4-triethylphenyl,2,3,5-triethylphenyl, 2,3,6-triethylphenyl, 2,4,5-triethylphenyl,2,4,6-triethylphenyl, 2-isopropylphenyl, 3-isopropylphenyl, and4-isopropylphenyl.

Another still further example of R¹ units includes substituted C₆ arylunits chosen from 2-aminophenyl, 2-(N-methylamino)phenyl,2-(N,N-dimethylamino)phenyl, 2-(N-ethylamino)phenyl,2-(N,N-diethylamino)phenyl, 3-aminophenyl, 3-(N-methylamino)phenyl,3-(N,N-dimethylamino)phenyl, 3-(N-ethylamino)phenyl,3-(N,N-diethylamino)phenyl, 4-aminophenyl, 4-(N-methylamino)phenyl,4-(N,N-dimethylamino)phenyl, 4-(N-ethylamino)phenyl, and4-(N,N-diethylamino)phenyl.

R¹ can comprise heteroaryl units. Non-limiting examples of heteroarylunits include:

i)

ii)

iii)

iv)

v)

vi)

vii)

viii)

ix)

x)

xi)

xii)

xiii)

and

xiv)

R¹ heteroaryl units can be substituted or unsubstituted. Non-limitingexamples of units that can substitute for hydrogen include units chosenfrom:

i) C₁-C₆ linear, branched, and cyclic alkyl;

ii) substituted or unsubstituted phenyl and benzyl;

iii) substituted of unsubstituted C₁-C₉ heteroaryl;

iv) —C(O)R⁹; and

v) —NHC(O)R⁹;

wherein R⁹ is C₁-C₆ linear and branched alkyl; C₁-C₆ linear and branchedalkoxy; or NHCH₂C(O)R¹⁰; R¹⁰ is chosen from hydrogen, methyl, ethyl, andtert-butyl.

An example of R¹ relates to units substituted by an alkyl unit chosenfrom methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, sec-butyl,and tert-butyl.

Another example of R¹ includes units that are substituted by substitutedor unsubstituted phenyl and benzyl, wherein the phenyl and benzylsubstitutions are chosen from one or more:

i) halogen;

ii) C₁-C₃ alkyl;

iii) C₁-C₃ alkoxy;

iv) —CO₂R¹¹; and

v) —NHCOR¹⁶;

wherein R¹¹ and R¹⁶ are each independently hydrogen, methyl, or ethyl.

Another example of R¹ relates to phenyl and benzyl units substituted bya carboxy unit having the formula —C(O)R⁹; R⁹ is chosen from methyl,methoxy, ethyl, and ethoxy.

A further example of R¹ includes phenyl and benzyl units substituted byan amide unit having the formula —NHC(O)R⁹; R⁹ is chosen from methyl,methoxy, ethyl, ethoxy, tert-butyl, and tert-butoxy.

A yet further example of R¹ includes phenyl and benzyl units substitutedby one or more fluoro or chloro units.

L Units

L is a linking unit which is present when the index n is equal to 1, butis absent when the index n is equal to 0. L units have the formula:

-[Q]_(y)[C(R^(5a)R^(5b))]_(x)[Q¹]_(z)C(R^(6a)R^(6b))]_(w)—

wherein Q and Q¹ are each independently:

i) —C(O)—;

ii) —NH—;

iii) —C(O)NH—;

iv) —NHC(O)—;

v) —NHC(O)NH—;

vi) —NHC(O)O—;

vii) —C(O)O—;

viii) —C(O)NHC(O)—;

ix) —O—;

x) —S—;

xi) —SO₂—;

xii) —C(═NH)—;

xiii) —C(═NH)NH—;

xiv) —NHC(═NH)—; or

xv) —NHC(═NH)NH—.

When the index y is equal to 1, Q is present. When the index y is equalto 0, Q is absent. When the index z is equal to 1, Q¹ is present. Whenthe index z is equal to 0, Q¹ is absent.

R^(5a) and R^(5b) are each independently:

i) hydrogen;

ii) hydroxy;

iii) halogen;

iv) C₁-C₆ substituted or unsubstituted linear or branched alkyl; or

v) a unit having the formula:

—[C(R^(7a)R^(7b))]_(t)R⁸

wherein R^(7a) and R^(7b) are each independently:

i) hydrogen; or

ii) substituted or unsubstituted C₁-C₆ linear, branched, or cyclicalkyl.

R⁸ is:

i) hydrogen;

ii) substituted or unsubstituted C₁-C₆ linear, branched, or cyclicalkyl;

iii) substituted or unsubstituted C₆ or C₁₀ aryl;

iv) substituted or unsubstituted C₁-C₉ heteroaryl; or

v) substituted or unsubstituted C₁-C₉ heterocyclic.

R^(6a) and R^(6b) are each independently:

i) hydrogen; or

ii) C₁-C₄ linear or branched alkyl.

The indices t, w and x are each independently from 0 to 4.

The following are non-limiting examples of units that can substitute forone or more hydrogen atoms on R^(5a), R^(5b), R^(7a), R^(7b), and R⁸units. The following substituents, as well as others not hereindescribed, are each independently chosen:

-   -   i) C₁-C₁₂ linear, branched, or cyclic alkyl, alkenyl, and        alkynyl; methyl (C₁), ethyl (C₂), ethenyl (C₂), ethynyl (C₂),        n-propyl (C₃), iso-propyl (C₃), cyclopropyl (C₃), 3-propenyl        (C₃), 1-propenyl (also 2-methylethenyl) (C₃), isopropenyl (also        2-methylethen-2-yl) (C₃), prop-2-ynyl (also propargyl) (C₃),        propyn-1-yl (C₃), n-butyl (C₄), sec-butyl (C₄), iso-butyl (C₄),        tert-butyl (C₄), cyclobutyl (C₄), buten-4-yl (C₄), cyclopentyl        (C₅), cyclohexyl (C₆);    -   ii) substituted or unsubstituted C₆ or C₁₀ aryl; for example,        phenyl, naphthyl (also referred to herein as naphthylen-1-yl        (C₁₀) or naphthylen-2-yl (C₁₀));    -   iii) substituted or unsubstituted C₆ or C₁₀ alkylenearyl; for        example, benzyl, 2-phenylethyl, naphthylen-2-ylmethyl;    -   iv) substituted or unsubstituted C₁-C₉ heterocyclic rings; as        described herein below;    -   v) substituted or unsubstituted C₁-C₉ heteroaryl rings; as        described herein below;    -   vi) (CR^(41a)R^(41b))_(r)OR⁴⁰; for example, —OH, —CH₂OH, —OCH₃,        —CH₂OCH₃, —OCH₂CH₃, —CH₂OCH₂CH₃, —OCH₂CH₂CH₃, and        —CH₂OCH₂CH₂CH₃;    -   vii) —(CR^(41a)R^(41b))_(r)C(O)R⁴⁰; for example, —COCH₃,        —CH₂COCH₃, —COCH₂CH₃, —CH₂COCH₂CH₃, —COCH₂CH₂CH₃, and        —CH₂COCH₂CH₂CH₃;    -   viii) (CR^(41a)R^(41b))_(r)C(O)OR⁴⁰; for example, —CO₂CH₃,        —CH₂CO₂CH₃, —CO₂CH₂CH₃, —CH₂CO₂CH₂CH₃, —CO₂CH₂CH₂CH₃, and        —CH₂CO₂CH₂CH₂CH₃;    -   xiv) —(CR^(41a)R^(41b))_(r)C(O)N(R⁴⁰)₂; for example, —CONH₂,        —CH₂CONH₂, —CONHCH₃, —CH₂CONHCH₃, —CON(CH₃)₂, and —CH₂CON(CH₃)₂;    -   x) —(CR^(41a)R^(41b))_(r)N(R⁴⁰)₂; for example, —NH₂, —CH₂NH₂,        —NHCH₃, —CH₂NHCH₃, —N(CH₃)₂, and —CH₂N(CH₃)₂;    -   xi) halogen; —F, —Cl, —Br, and —I;    -   xii) (CR^(41a)R^(41b))_(r)CN;    -   xiii) —(CR^(41a)R^(41b))_(r)NO₂;    -   xiv) —(CH_(j′)X_(k′))_(h)CH_(j)X_(k); wherein X is halogen, the        index j is an integer from 0 to 2, j+k=3, the index j′ is an        integer from 0 to 2, j′+k′=2, the index h is from 0 to 6; for        example, —CH₂F, —CHF₂, —CF₃, —CH₂CF₃, —CHFCF₃, —CCl₃, or —CBr₃;    -   xv) —(CR^(41a)R^(41b))_(r)SR⁴⁰; —SH, —CH₂SH, —SCH₃, —CH₂SCH₃,        —SC₆H₅, and —CH₂SC₆H₅;    -   xvi) —(CR^(41a)R^(41b))_(r)SO₂R⁴⁰; for example, —SO₂H, —CH₂SO₂H,        —SO₂CH₃, —CH₂SO₂CH₃, —SO₂C₆H₅, and —CH₂SO₂C₆H₅; and    -   xvii) —(CR^(41a)R^(41b))_(r)SO₃R⁴⁰; for example, —SO₃H,        —CH₂SO₃H, —SO₃CH₃, —CH₂SO₃CH₃, —SO₃C₆H₅, and —CH₂SO₃C₆H₅;        wherein each R⁴⁰ is independently hydrogen, substituted or        unsubstituted C₁-C₆ linear, branched, or cyclic alkyl, phenyl,        benzyl, heterocyclic, or heteroaryl; or two R⁴⁰ units can be        taken together to form a ring comprising 3-7 atoms; R^(41a) and        R^(41b) are each independently hydrogen or C₁-C₄ linear or        branched alkyl; the index r is from 0 to 4.

One aspect of L units relates to units having the formula:

—C(O)[C(R^(5a)R^(5b))]_(X)NHC(O)—

wherein R^(5a) is hydrogen, substituted or unsubstituted C₁-C₄ alkyl,substituted or unsubstituted phenyl, and substituted or unsubstitutedheteroaryl; and the index x is 1 or 2. One embodiment relates to linkingunits having the formula:

i) —C(O)[C(R^(5a)H)]NHC(O)O—;

ii) —C(O)[C(R^(5a)H)][CH₂]NHC(O)O—;

ii) —C(O)[CH₂][C(R^(5a)H)]NHC(O)O—;

iv) —C(O)[C(R^(5a)H)]NHC(O)—;

v) —C(O)[C(R^(5a)H)][CH₂]NHC(O)—; or

vi) —C(O)[CH₂][C(R^(5a)H)]NHC(O)—;

wherein R^(5a) is:

i) hydrogen;

ii) methyl;

iii) ethyl;

iv) isopropyl;

v) phenyl;

yl) benzyl;

vii) 4-hydroxybenzyl;

viii) hydroxymethyl; or

ix) 1-hydroxyethyl.

When the index x is equal to 1, this embodiment provides the followingnon-limiting examples of L units:

When the index x is equal to 2, this embodiment provides the followingnon-limiting examples of L units:

Another embodiment of L units includes units wherein Q is —C(O)—, theindices x and z are equal to 0, w is equal to 1 or 2, a first R^(6a)unit chosen from phenyl, 2-fluorophenyl, 3-fluorophenyl, 4-fluorophenyl,2,3-difluorophenyl, 3,4-difluorophenyl, 3,5-difluorophenyl,2-chlorophenyl, 3-chlorophenyl, 4-chlorophenyl, 2,3-dichlorophenyl,3,4-dichlorophenyl, 3,5-dichlorophenyl, 2-hydroxyphenyl,3-hydroxyphenyl, 4-hydroxyphenyl, 2-methoxyphenyl, 3-methoxyphenyl,4-methoxyphenyl, 2,3-dimethoxyphenyl, 3,4-dimethoxyphenyl, and3,5-dimethoxyphenyl; a second R^(6a) unit is hydrogen and R^(6b) unitsare hydrogen. For example a linking unit having the formula:

A further example of this embodiment of L includes a first R^(6a) unitas depicted herein above that is a substituted or unsubstitutedheteroaryl unit as described herein above.

A yet further example of this embodiment of L includes units having theformula:

—C(O)[C(R^(6a)R^(6b))]_(w)—;

wherein R^(6a) and R^(6b) are hydrogen and the index w is equal to 1 or2; said units chosen from:

i) —C(O)CH₂—; and

ii) —C(O)CH₂CH₂—.

Another embodiment of L units includes units having the formula:

—C(O)[C(R^(5a)R^(5b))]_(x)C(O)—;

wherein R^(5a) and R^(5b) are hydrogen and the index x is equal to 1 or2; said units chosen from:

i) —C(O)CH₂C(O)—; and

ii) —C(O)CH₂CH₂C(O)—.

A still further embodiment of L units includes units having the formula:

—C(O)NH[C(R^(5a)R^(5b))]_(x)—;

wherein R^(5a) and R^(5b) are hydrogen and the index w is equal to 0, 1or 2; said units chosen from:

ii) —C(O)NH—;

ii) —C(O)NHCH₂—; and

iii) —C(O)NHCH₂CH₂—.

A yet still further example of L units includes units having theformula:

—SO₂[C(R^(6a)R^(6b))]_(w)—;

wherein R^(8a) and R^(8b) are hydrogen or methyl and the index w isequal to 0, 1 or 2; said units chosen from:

i) —SO₂—;

ii) —SO₂CH₂—; and

iii) —SO₂CH₂CH₂—.

Vascular Leakage Control

The disclosed compounds (analogs) are arranged into several Categoriesto assist the formulator in applying a rational synthetic strategy forthe preparation of analogs which are not expressly exampled herein. Thearrangement into categories does not imply increased or decreasedefficacy for any of the compositions of matter described herein.

A described herein above the disclosed compounds include allpharmaceutically acceptable salt forms. A compound having the formula:

can form salts, for example, a salt of the sulfamic acid:

The compounds can also exist in a zwitterionic form, for example:

or as a salt of a strong acid, for example:

The first aspect of Category I of the present disclosure relates tocompounds wherein R is a substituted or unsubstituted thiazol-2-yl unithaving the formula:

one embodiment of which relates to inhibitors having the formula:

wherein R units are thiazol-2-yl units, that when substituted, aresubstituted with R² and R³ units. R and R^(5a) units are furtherdescribed in Table I.

TABLE I No. R R^(5a) A1 thiazol-2-yl (S)-benzyl A2 4-methylthiazol-2-yl(S)-benzyl A3 4-ethylthiazol-2-yl (S)-benzyl A4 4-propylthiazol-2-yl(S)-benzyl A5 4-iso-propylthiazol-2-yl (S)-benzyl A64-cyclopropylthiazol-2-yl (S)-benzyl A7 4-butylthiazol-2-yl (S)-benzylA8 4-tert-butylthiazol-2-yl (S)-benzyl A9 4-cyclohexylthiazol-2-yl(S)-benzyl A10 4-(2,2,2-trifluoroethyl)thiazol-2-yl (S)-benzyl A114-(3,3,3-trifluoropropyl)thiazol-2-yl (S)-benzyl A124-(2,2-difluorocyclopropyl)thiazol-2-yl (S)-benzyl A134-(methoxymethyl)thiazol-2-yl (S)-benzyl A14 4-(carboxylic acid ethylester)thiazol-2-yl (S)-benzyl A15 4,5-dimethylthiazol-2-yl (S)-benzylA16 4-methyl-5-ethylthiazol-2-yl (S)-benzyl A17 4-phenylthiazol-2-yl(S)-benzyl A18 4-(4-chlorophenyl)thiazol-2-yl (S)-benzyl A194-(3,4-dimethylphenyl)thiazol-2-yl (S)-benzyl A204-methyl-5-phenylthiazol-2-yl (S)-benzyl A214-(thiophen-2-yl)thiazol-2-yl (S)-benzyl A224-(thiophen-3-yl)thiazol-2-yl (S)-benzyl A234-(5-chlorothiophen-2-yl)thiazol-2-yl (S)-benzyl A245,6-dihydro-4H-cyclopenta[d]thiazol-2-yl (S)-benzyl A254,5,6,7-tetrahydrobenzo[d]thiazol-2-yl (S)-benzyl

The compounds encompassed within the first aspect of Category I of thepresent disclosure can be prepared by the procedure outlined in Scheme Iand described in Example 1 herein below.

Example 14-{(S)-2-[(S)-2-(tert-Butoxycarbonylamino)-3-phenylpropanamido]-2-(4-ethylthiazol-2-yl)ethyl}phenylsulfamicacid (5)

Preparation of [1-(S)-carbamoyl-2-(4-nitrophenyl)ethyl-carbamic acidtert-butyl ester (1) To a 0° C. solution of2-(S)-tert-butoxycarbonylamino-3-(4-nitrophenyl)-propionic acid andN-methylmorpholine (1.1 mL, 9.65 mmol) in DMF (10 mL) is added dropwiseiso-butyl chloroformate (1.25 mL, 9.65 mmol). The mixture is stirred at0° C. for 20 minutes after which NH₃ (g) is passed through the reactionmixture for 30 minutes at 0° C. The reaction mixture is concentrated andthe residue dissolved in EtOAc, washed successively with 5% citric acid,water, 5% NaHCO₃, water and brine, dried (Na₂SO₄), filtered andconcentrated in vacuo to a residue that is triturated with a mixture ofEtOAc/petroleum ether to provide 2.2 g (74%) of the desired product as awhite solid.

Preparation of [2-(4-nitrophenyl)-1-(S)-thiocarbamoylethyl]carbamic acidtert-butyl ester (2): To a solution of[1-(S)-carbamoyl-2-(4-nitrophenyl)ethyl-carbamic acid tert-butyl ester,1, (0.400 g, 1.29 mmol) in THF (10 mL) is added Lawesson's reagent(0.262 g. 0.65 mmol). The reaction mixture is stirred for 3 hours andconcentrated to a residue which is purified over silica to provide 0.350g (83%) of the desired product. ¹H NMR (300 MHz, CDCl₃) δ 8.29 (s, 1H),8.10 (d. J=8.4 Hz, 2H), 8.01 (s, 1H), 7.42 (d, J=8.4 Hz, 2H), 5.70 (d,J=7.2 Hz, 1H), 4.85 (d, J=7.2 Hz, 1H), 3.11-3.30 (m, 1H), 1.21 (s, 9H).

Preparation of 1-(S)-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethyl amine(3): A mixture of [2-(4-nitrophenyl)-1-(S)-thiocarbamoylethyl]-carbamicacid tert-butyl ester, 2, (0.245 g, 0.753 mmol), 1-bromo-2-butanone(0.125 g, 0.828 mmol) in CH₃CN (5 mL) is refluxed 3 hours. The reactionmixture is cooled to room temperature and diethyl ether is added to thesolution and the precipitate which forms is removed by filtration. Thesolid is dried under vacuum to afford 0.242 g (90% yield) of the desiredproduct. ESI+MS 278 (M+1).

Preparation of{1-[1-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethylcarbamoyl]-2-phenylethyl}carbamic acid tert-butyl ester (4): To a solution of1-(5)-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethyl amine hydrobromide,3, (0.393 g, 1.1 mmol),(S)-(2-tert-butoxycarbonylamino)-3-phenylpropionic acid (0.220 g, 0.828mmol) and 1-hydroxybenzotriazole (HOBt) (0.127 g, 0.828 mmol) in DMF (10mL) at 0° C., is added 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide(EDCI) (0.159 g, 0.828 mmol) followed by diisopropylamine (0.204 g, 1.58mmol). The mixture is stirred at 0° C. for 30 minutes then at roomtemperature overnight. The reaction mixture is diluted with water andextracted with EtOAc. The combined organic phase is washed with 1 Naqueous HCl, 5% aqueous NaHCO₃, water and brine, and dried over Na₂SO₄.The solvent is removed in vacuo to afford 0.345 g of the desired productwhich is used without further purification. LC/MS ESI+525 (M+1).

Preparation of4-{(S)-2-[(5)-2-(tert-butoxycarbonylamino)-3-phenylpropanamido]-2-(4-ethylthiazol-2-yl)ethyl}phenylsulfamicacid ammonium salt (5):{1-[1-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethylcarbamoyl]-2-phenylethyl}carbamic acid tert-butyl ester, 4, (0.345 g) is dissolved in MeOH (4mL). A catalytic amount of Pd/C (10% w/w) is added and the mixture isstirred under a hydrogen atmosphere 2 hours. The reaction mixture isfiltered through a bed of CELITE™ and the solvent is removed underreduced pressure. The crude product is dissolved in pyridine (12 mL) andtreated with SO₃-pyridine (0.314 g). The reaction is stirred at roomtemperature for 5 minutes after which a 7% solution of NH₄OH (50 mL) isadded. The mixture is then concentrated and the resulting residue ispurified by reverse phase chromatography to afford 0.222 g of thedesired product as the ammonium salt. ¹H NMR (CD₃OD): δ 7.50-6.72 (m,10H), 5.44-5.42 (d, 1H, J=6.0 Hz), 4.34 (s, 1H), 3.34-2.79 (m, 4H),2.83-2.76 (q, 2H, J=7.2 Hz), 1.40 (s, 9H), 1.31 (t, 3H, J=7.5 Hz).

The disclosed inhibitors can also be isolated as the free acid. Anon-limiting example of this procedure is described herein below inExample 4.

The following is a non-limiting example of compounds encompassed withinthis embodiment of the first aspect of Category I of the presentdisclosure.

4-{(S)-2-[(R)-2-(tert-butoxycarbonylamino)-3-phenylpropanamido]-2-(4-ethylthiazol-2-yl)ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.22-7.02 (m, 10H), 5.39 (s, 1H), 4.34 (s, 1H),3.24-2.68 (m, 6H), 1.37 (s, 9H), 1.30 (t, 3H, J=7.5 Hz).

Another embodiment of this aspect of Category I relates to inhibitorshaving the formula:

wherein R units and R^(5a) units further described in Table II.

TABLE II No. R R^(5a) B26 thiazol-2-yl (S)-benzyl B274-methylthiazol-2-yl (S)-benzyl B28 4-ethylthiazol-2-yl (S)-benzyl B294-propylthiazol-2-yl (S)-benzyl B30 4-iso-propylthiazol-2-yl (S)-benzylB31 4-cyclopropylthiazol-2-yl (S)-benzyl B32 4-butylthiazol-2-yl(S)-benzyl B33 4-tert-butylthiazol-2-yl (S)-benzyl B344-cyclohexylthiazol-2-yl (S)-benzyl B354-(2,2,2-trifluoroethyl)thiazol-2-yl (S)-benzyl B364-(3,3,3-trifluoropropyl)thiazol-2-yl (S)-benzyl B374-(2,2-difluorocyclopropyl)thiazol-2-yl (S)-benzyl B384-(methoxymethyl)thiazol-2-yl (S)-benzyl B39 4-(carboxylic acid ethylester)thiazol-2-yl (S)-benzyl B40 4,5-dimethylthiazol-2-yl (S)-benzylB41 4-methyl-5-ethylthiazol-2-yl (S)-benzyl B42 4-phenylthiazol-2-yl(S)-benzyl B43 4-(4-chlorophenyl)thiazol-2-yl (S)-benzyl B444-(3,4-dimethylphenyl)thiazol-2-yl (S)-benzyl B454-methyl-5-phenylthiazol-2-yl (S)-benzyl B464-(thiophen-2-yl)thiazol-2-yl (S)-benzyl B474-(thiophen-3-yl)thiazol-2-yl (S)-benzyl B484-(5-chlorothiophen-2-yl)thiazol-2-yl (S)-benzyl B495,6-dihydro-4H-cyclopenta[d]thiazol-2-yl (S)-benzyl B504,5,6,7-tetrahydrobenzo[d]thiazol-2-yl (S)-benzyl

The compounds of this embodiment can be prepared according to theprocedure outlined above in Scheme I and described in Example 1 bysubstituting the appropriate Boc-β-amino acid for(S)-(2-tert-butoxycarbonylamino)-3-phenylpropionic acid in step (d).

The following are non-limiting examples of compounds according to thisembodiment.

{1-[1-(4-Ethylthiazol-2-yl)-(S)-2-(4-sulfoaminophenyl)ethylcarbamoyl]-(S)-2-phenylethyl}methylcarbamic acid tert-butyl ester: ¹H NMR (300 MHz, MeOH-d₄) δ 8.36 (d,J=8.1 Hz, 1H), 7.04-7.22 (m, 9H), 5.45 (s, 1H), 3.01-3.26 (m, 2H),2.60-2.88 (m, 4H), 2.33 (s, 3H), 1.30 (s, 9H).

{1-[1-(4-Phenylthiazol-2-yl)-(S)-2-(4-sulfoaminophenyl)ethylcarbamoyl]-(S)-2-phenylethyl}methylcarbamic acid tert-butyl ester: ¹H NMR (300 MHz, MeOH-d₄) δ 8.20 (d,J=8.1 Hz, 1H), 7.96-7.99 (m, 2H), 7.48-7.52 (m, 3H), 7.00-7.23 (m, 7H),6.89 (s, 1H), 5.28 (q, J=7.5 Hz, 1H), 4.33 (t, J=6.6 Hz, 1H), 3.09-3.26(m, 2H), 3.34 (dd, J=13.2 and 8.4 Hz, 1H), 2.82 (dd, J=13.2 and 8.4 Hz,1H), 1.38 (s, 9H).

The second aspect of Category I of the present disclosure relates tocompounds wherein R is a substituted or unsubstituted thiazol-4-ylhaving the formula:

one embodiment of which relates to inhibitors having the formula:

wherein R units and R^(5a) units further described in Table III.

TABLE III No. R R^(5a) C51 thiazol-4-yl (S)-benzyl C522-methylthiazol-4-yl (S)-benzyl C53 2-ethylthiazol-4-yl (S)-benzyl C542-propylthiazol-4-yl (S)-benzyl C55 2-iso-propylthiazol-4-yl (S)-benzylC56 2-cyclopropylthiazol-4-yl (S)-benzyl C57 2-butylthiazol-4-yl(S)-benzyl C58 2-tert-butylthiazol-4-yl (S)-benzyl C592-cyclohexylthiazol-4-yl (S)-benzyl C602-(2,2,2-trifluoroethyl)thiazol-4-yl (S)-benzyl C612-(3,3,3-trifluoropropyl)thiazol-4-yl (S)-benzyl C622-(2,2-difluorocyclopropyl)thiazol-4-yl (S)-benzyl C632-phenylthiazol-4-yl (S)-benzyl C64 2-(4-chlorophenyl)thiazol-4-yl(S)-benzyl C65 2-(3,4-dimethylphenyl)thiazol-4-yl (S)-benzyl C662-(thiophen-2-yl)thiazol-4-yl (S)-benzyl C672-(thiophen-3-yl)thiazol-4-yl (S)-benzyl C682-(3-chlorothiophen-2-yl)thiazol-4-yl (S)-benzyl C692-(3-methylthiophen-2-yl)thiazol-4-yl (S)-benzyl C702-(2-methylthiazol-4-yl)thiazol-4-yl (S)-benzyl C712-(furan-2-yl)thiazol-4-yl (S)-benzyl C72 2-(pyrazin-2-yl)thiazol-4-yl(S)-benzyl C73 2-[(2-methyl)pyridin-5-yl]thiazol-4-yl (S)-benzyl C742-(4-chlorobenzenesulfonylmethyl)thiazol-4-yl (S)-benzyl C752-(tert-butylsulfonylmethyl)thiazol-4-yl (S)-benzyl

The compounds encompassed within the second aspect of Category I of thepresent disclosure can be prepared by the procedure outlined in SchemeII and described in Example 2 herein below.

Example 24-{(S)-2-(S)-2-(tert-Butoxycarbonylamino)-3-phenylpropanamido-2-(2-phenylthiazol-4-yl)}phenylsulfamicacid (9)

Preparation of (S)-[3-diazo-1-(4-nitrobenzyl)-2-oxo-propyl]-carbamicacid tert-butyl ester (6): To a 0° C. solution of2-(S)-tert-butoxycarbonylamino-3-(4-nitrophenyl)-propionic acid (1.20 g,4.0 mmol) in THF (20 mL) is added dropwise triethylamine (0.61 mL, 4.4mmol) followed by iso-butyl chloroformate (0.57 mL, 4.4 mmol). Thereaction mixture is stirred at 0° C. for 20 minutes and filtered. Thefiltrate is treated with an ether solution of diazomethane (˜16 mmol) at0° C. The reaction mixture is stirred at room temperature for 3 hoursthen concentrated in vacuo. The resulting residue is dissolved in EtOAcand washed successively with water and brine, dried (Na₂SO₄), filteredand concentrated. The residue is purified over silica (hexane/EtOAc 2:1)to afford 1.1 g (82% yield) of the desired product as a slightly yellowsolid. ¹H NMR (300 MHz, CDCl₃) δ 8.16 (d, J=8.7 Hz, 2H), 7.39 (d, J=8.7Hz, 2H), 5.39 (s, 1H), 5.16 (d, J=6.3 Hz, 1H), 4.49 (s, 1H), 3.25 (dd,J=13.8 and 6.6, 1H), 3.06 (dd, J=13.5 and 6.9 Hz, 1H), 1.41 (s, 9H).

Preparation of (S)-tert-butyl4-bromo-1-(4-nitrophenyl)-3-oxobutan-2-ylcarbamate (7): To a 0° C.solution of (S)-[3-diazo-1-(4-nitrobenzyl)-2-oxo-propyl]-carbamic acidtert-butyl ester, 6, (0.350 g, 1.04 mmol) in THF (5 mL) is addeddropwise 48% aq. HBr (0.14 mL, 1.25 mmol). The reaction mixture isstirred at 0° C. for 1.5 hours then the reaction is quenched at 0° C.with sat. Na₂CO₃. The mixture is extracted with EtOAc (3×25 mL) and thecombined organic extracts are washed with brine, dried (Na₂SO₄),filtered and concentrated to obtain 0.400 g of the product which is usedin the next step without further purification. ¹H NMR (300 MHz, CDCl₃) δ8.20 (d, J=8.4 Hz, 2H), 7.39 (d, J=8.4 Hz, 2H), 5.06 (d, J=7.8 Hz, 1H),4.80 (q, J=6.3 Hz, 1H), 4.04 (s, 2H), 1.42 (s, 9H).

Preparation of tert-butyl(S)-1-(S)-2-(4-nitrophenyl)-1-(2-phenylthiazole-4-yl)ethylamino-1-oxo-3-phenylpropan-2-ylcarbamate(8): A mixture of thiobenzamide (0.117 g, 0.85 mmol) and (S)-tert-butyl4-bromo-1-(4-nitrophenyl)-3-oxobutan-2-ylcarbamate, 7, (0.300 g, 0.77mmol) in CH₃CN (4 mL) is refluxed 2 hours. The reaction mixture iscooled to room temperature and diethyl ether is added to precipitate theintermediate 2-(nitrophenyl)-(S)-1-(4-phenylthiazol-2-yl)ethylaminewhich is isolated by filtration as the hydrobromide salt. Thehydrobromide salt is dissolved in DMF (3 mL) together withdiisoproylethylamine (0.42 mL, 2.31 mmol), 1-hydroxybenzotriazole (0.118g, 0.79 mmol) and (S)-(2-tert-butoxycarbonyl-amino)-3-phenylpropionicacid (0.212 g, 0.80 mmol). The mixture is stirred at 0° C. for 30minutes then at room temperature overnight. The reaction mixture isdiluted with water and extracted with EtOAc. The combined organic phaseis washed with 1 N aqueous HCl, 5% aqueous NaHCO₃, water and brine, anddried over Na₂SO₄. The solvent is removed in vacuo to afford 0.395 g(90% yield) of the desired product which is used without furtherpurification.

LC/MS ESI+573 (M+1).

Preparation of4-{(S)-2-(S)-2-(tert-butoxycarbonyl)-3-phenylpropaneamido-2-(2-phenylthiazole-4-yl)}phenylsulfamicacid (9): tert-butyl(S)-1-(S)-2-(4-nitrophenyl)-1-(2-phenylthiazole-4-yl)ethylamino-1-oxo-3-phenylpropan-2-ylcarbamate,8, (0.360 g) is dissolved in MeOH (4 mL). A catalytic amount of Pd/C(10% w/w) is added and the mixture is stirred under a hydrogenatmosphere 12 hours. The reaction mixture is filtered through a bed ofCELITE™ and the solvent is removed under reduced pressure. The crudeproduct is dissolved in pyridine (12 mL) and treated with SO₃-pyridine(0.296 g). The reaction is stirred at room temperature for 5 minutesafter which a 7% solution of NH₄OH (10 mL) is added. The mixture is thenconcentrated and the resulting residue is purified by reverse phasechromatography to afford 0.050 g of the desired product as the ammoniumsalt. ¹H NMR (300 MHz, MeOH-d₄) δ 8.20 (d, J=8.1 Hz, 1H), 7.96-7.99 (m,2H), 7.48-7.52 (m, 3H), 7.00-7.23 (m, 7H), 6.89 (s, 1H), 5.28 (q, J=7.5Hz, 1H), 4.33 (t, J=6.6 Hz, 1H), 3.09-3.26 (m, 2H), 3.34 (dd, J=13.2 and8.4 Hz, 1H), 2.82 (dd, J=13.2 and 8.4 Hz, 1H), 1.38 (s, 9H).

The first aspect of Category II of the present disclosure relates tocompounds wherein R is a substituted or unsubstituted thiazol-4-yl unithaving the formula:

one embodiment of which relates to inhibitors having the formula:

wherein R units are thiazol-4-yl units, that when substituted, aresubstituted with R⁴ units. R and R^(5a) units are further described inTable IV.

TABLE IV No. R R^(5a) D76 thiazol-4-yl (S)-benzyl D772-methylthiazol-4-yl (S)-benzyl D78 2-ethylthiazol-4-yl (S)-benzyl D792-propylthiazol-4-yl (S)-benzyl D80 2-iso-propylthiazol-4-yl (S)-benzylD81 2-cyclopropylthiazol-4-yl (S)-benzyl D82 2-butylthiazol-4-yl(S)-benzyl D83 2-tert-butylthiazol-4-yl (S)-benzyl D842-cyclohexylthiazol-4-yl (S)-benzyl D852-(2,2,2-trifluoroethyl)thiazol-4-yl (S)-benzyl D862-(3,3,3-trifluoropropyl)thiazol-4-yl (S)-benzyl D872-(2,2-difluorocyclopropyl)thiazol-4-yl (S)-benzyl D882-phenylthiazol-4-yl (S)-benzyl D89 2-(4-chlorophenyl)thiazol-4-yl(S)-benzyl D90 2-(3,4-dimethylphenyl)thiazol-4-yl (S)-benzyl D912-(thiophen-2-yl)thiazol-4-yl (S)-benzyl D922-(thiophen-3-yl)thiazol-4-yl (S)-benzyl D932-(3-chlorothiophen-2-yl)thiazol-4-yl (S)-benzyl D942-(3-methylthiophen-2-yl)thiazol-4-yl (S)-benzyl D952-(2-methylthiazol-4-yl)thiazol-4-yl (S)-benzyl D962-(furan-2-yl)thiazol-4-yl (S)-benzyl D97 2-(pyrazin-2-yl)thiazol-4-yl(S)-benzyl D98 2-[(2-methyl)pyridin-5-yl]thiazol-4-yl (S)-benzyl D992-(4-chlorobenzenesulfonylmethyl)thiazol-4-yl (S)-benzyl D1002-(tert-butylsulfonylmethyl)thiazol-4-yl (S)-benzyl

The compounds encompassed within the second aspect of Category II of thepresent disclosure can be prepared by the procedure outlined in SchemeIII and described in Example 3 herein below

Example 34-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-(2-ethylthiazol-4-yl)ethyl}phenylsulfamicacid (13)

Preparation of methyl(S)-1-[(S)-1-(2-ethylthiazole-4-yl)-2-(4-nitrophenyl)-ethyl]amino-1-oxo-3-phenylpropane-2-ylcarbamate(12): A mixture of propanethioamide (69 mg, 0.78 mmol) and(S)-tert-butyl 4-bromo-1-(4-nitrophenyl)-3-oxobutan-2-ylcarbamate, 7,(0.300 g, 0.77 mmol) in CH₃CN (4 mL) is refluxed for 2 hours. Thereaction mixture is cooled to room temperature and diethyl ether isadded to precipitate the intermediate2-(nitrophenyl)-(S)-1-(4-ethylthiazol-2-yl)ethylamine which is isolatedby filtration as the hydrobromide salt. The hydrobromide salt isdissolved in DMF (8 mL) together with diisoproylethylamine (0.38 mL,2.13 mmol), 1-hydroxybenzotriazole (107 mg, 0.71 mmol) and(S)-(2-methoxycarbonyl-amino)-3-phenylpropionic acid (175 mg, 0.78mmol). The mixture is stirred at 0° C. for 30 minutes then at roomtemperature overnight. The reaction mixture is diluted with water andextracted with EtOAc. The combined organic phase is washed with 1 Naqueous HCl, 5% aqueous NaHCO₃, water and brine, and dried over Na₂SO₄.The solvent is removed in vacuo to afford 0.300 g (81% yield) of thedesired product which is used without further purification. LC/MS ESI+MS483 (M+1).

Preparation of4-((S)-2-(S)-2-(methoxycarbonylamino)-3-phenylpropanamido)-2-(2-ethylthiazol-4-yl)ethyl)phenylsulfamicacid ammonium salt (13): tert-Butyl(S)-1-(S)-2-(4-nitrophenyl)-1-(2-ethylthiazole-4-yl)ethylamino-1-oxo-3-phenylpropan-2-ylcarbamate,12, (0.300 g) is dissolved in MeOH (4 mL). A catalytic amount of Pd/C(10% w/w) is added and the mixture is stirred under a hydrogenatmosphere 18 hours. The reaction mixture is filtered through a bed ofCELITE™ and the solvent is removed under reduced pressure. The crudeproduct is dissolved in pyridine (12 mL) and treated with SO₃-pyridine(223 mg, 1.40 mmol). The reaction is stirred at room temperature for 5minutes after which a 7% solution of NH₄OH (12 mL) is added. The mixtureis then concentrated and the resulting residue is purified by reversephase chromatography to afford 25 mg of the desired product as theammonium salt. ¹H NMR (300 MHz, MeOH-d₄) δ 7.14-7.24 (m, 6H), 6.97-7.0(m, 4H), 6.62 (s, 1H), 5.10-5.30 (m, 1H), 4.36 (t, J=7.2 Hz, 1H), 3.63(s, 3H), 3.14 (dd, J=13.5 and 6.3 Hz, 1H), 2.93-3.07 (m, 5H), 2.81 (dd,J=13.5 and 6.3 HZ, 1H), 1.39 (t, J=7.8 Hz, 3H).

In another iteration of the process of the present disclosure, compound13, as well as the other analogs which comprise the present disclosure,can be isolated as the free acid by adapting the procedure describedherein below.

Example 44-((S)-2-((S)-2-(Methoxycarbonylamino)-3-phenylpropanamido)-2-(2-ethylthiazol-4-yl)ethyl)phenylsulfamicacid [Free Acid Form] (13)

Preparation of{1-[2-(S)-(4-(S)-aminophenyl)-1-(2-ethylthiazol-4-yl)ethyl-carbamoyl]-2-phenylethyl}-carbamicacid methyl ester (12a): A Parr hydrogenation vessel is charged withtert-butyl(S)-1-(S)-2-(4-nitrophenyl)-1-(2-ethylthiazole-4-yl)ethylamino-1-oxo-3-phenylpropan-2-ylcarbamate,12, (18.05 g, 37.4 mmol, 1.0 eq) and Pd/C (10% Pd on C, 50% wet,Degussa-type E101 NE/W, 2.68 g, 15 wt %) as solids. MeOH (270 mL, 15mL/g) is added to provide a suspension. The vessel is put on a Parrhydrogenation apparatus. The vessel is submitted to a fill/vacuumevacuate process with N₂ (3×20 psi) to inert, followed by the sameprocedure with H₂ (3×40 psi). The vessel is filled with H₂ and thevessel is shaken under 40 psi H₂ for ˜40 hr. The vessel is evacuated andthe atmosphere is purged with N₂ (5×20 psi). An aliquot is filtered andanalyzed by HPLC to insure complete conversion. The suspension isfiltered through a pad of celite to remove the catalyst, and thehomogeneous yellow filtrate is concentrated by rotary evaporation toafford 16.06 g (95% yield) of the desired product as a tan solid, whichis used without further purification.

Preparation of4-((S)-2-(S)-2-(methoxycarbonyl)-3-phenylpropanamido)-2-(2-ethylthiazol-4-yl)ethyl)phenylsulfamicacid (13): A 100 mL RBF is charged with{1-[2-(S)-(4-(S)-aminophenyl)-1-(2-ethylthiazol-4-yl)ethyl-carbamoyl]-2-phenylethyl}-carbamicacid methyl ester, 12a, (10.36 g, 22.9 mmol, 1.0 eq) prepared in thestep described herein above. Acetonitrile (50 mL, 5 mL/g) is added andthe yellow suspension is stirred at room temperature. A second 3-necked500 mL RBF is charged with SO₃. pyr (5.13 g, 32.2 mmol, 1.4 eq) andacetonitrile (50 mL 5 mL/g) and the white suspension is stirred at roomtemperature. Both suspensions are gently heated until the reactionsolution containing{1-[2-(S)-(4-(S)-aminophenyl)-1-(2-ethylthiazol-4-yl)ethyl-carbamoyl]-2-phenylethyl}-carbamicacid methyl ester becomes red-orange in color (typically for thisexample about 44° C.). This substrate containing solution is poured inone portion into the stirring suspension of SO₃. pyr at 35° C. Theresulting opaque mixture (39° C.) is stirred vigorously while allowed toslowly cool to room temperature. After stirring for 45 min, the reactionis determined to be complete by HPLC. H₂O (200 mL, 20 mL/g) is added tothe orange suspension to provide a yellow-orange homogeneous solutionhaving a pH of approximately 2.4. Concentrated H₃PO₄ is added slowlyover 12 minutes to lower the pH to approximately 1.4. During this pHadjustment, an off-white precipitate is formed and the solution isstirred at room temperature for 1 hr. The suspension is filtered and thefilter cake is washed with the filtrate. The filter cake is air-dried onthe filter overnight to afford 10.89 g (89% yield) of the desiredproduct as a tan solid.

The following are further non-limiting examples of the second aspect ofCategory II of the present disclosure.

4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-(2-methylthiazol-4-yl)ethyl}phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 8.15 (d, J=8.4 Hz, 1H), 7.16-7.25 (m,5H), 6.97-7.10 (m, 4H), 6.61 (s, 1H), 5.00-5.24 (m, 1H), 4.36 (t, J=7.2Hz, 1H), 3.64 (s, 2H), 3.11-3.19 (s, 1H), 2.92-3.04 (s, 2H), 2.81 (dd,J=13.5 and 8.1 Hz, 1H), 2.75 (s, 3H).

4-{(S)-2-(2-Ethylthiazole-4-yl)-2-[(S)-2-(methoxycarbonylamino)-3-phenylpropan-amido]ethyl}phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.16-7.29 (m, 5H), 7.02-7.12 (m, 4H),6.83 (s, 1H), 5.10-5.35 (m, 1H), 3.52-3.67 (m, 3H), 3.18-3.25 (m, 2H),3.05 (q, J=7.5 Hz, 2H), 2.82-2.95 (m, 2H), 2.65 (s, 3H), 1.39 (t, J=7.5Hz, 3H).

4-{(S)-2-(2-Isopropylthiazol-4-yl)-2-[(S)-2-(methoxycarbonylamino)-3-phenylpropan-amido]ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD) δ 8.16 (d, 1H, J=8.7 Hz), 7.22-7.13 (m, 3H), 7.07(d, 1H, J=8.4 Hz), 6.96 (d, 1H, J=8.1 Hz), 6.62 (s, 1H), 5.19 (t, 1H,J=7.2 Hz), 4.36 (t, 1H, J=7.8 Hz), 3.63 (s, 3H), 3.08 (1H, A of ABX,J=3.6, 14.5 Hz), 2.99 (1H, B of ABX, J=7.2, 13.8 Hz), 2.85-2.78 (m, 1H),1.41 (d, 6H, J=6.9 Hz).

4-{(S)-2-(2-Cyclopropylthiazol-4-yl)-2-[(S)-2-(methoxycarbonylamino)-3-phenylpropanamido]ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.15-7.02 (m, 5H), 6.96-6.93 (d, 2H, J=8.4 Hz),6.86-6.83 (d, 2H, J=8.3 Hz), 6.39 (s, 1H), 5.01 (t, 1H, J=5.0 Hz), 4.22(t, 1H, J=7.4 Hz), 3.51 (s, 3H), 2.98-2.69 (m, 2H), 2.22-2.21 (m, 1H),1.06-1.02 (m, 2H), 0.92-0.88 (m, 2H).

4-{(S)-2-{2-[(4-Chlorophenylsulfonyl)methyl]thiazol-4-yl}-2-[(S)-2-(methoxy-carbonylamino)-3-phenylpropanamido]ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.96-7.93 (d, 2H, J=8.6 Hz), 7.83-7.80 (d, 2H,J=8.6 Hz), 7.44-7.34 (m, 5H), 7.29-7.27 (d, 2H, J=8.4 Hz), 7.14-7.11 (d,2H, J=8.4 Hz), 6.97 (s, 1H), 5.31 (t, 1H, J=6.8 Hz), 5.22-5.15 (m, 2H),4.55 (t, 1H, J=7.3 Hz), 3.84 (s, 3H), 3.20-2.96 (m, 4H).

4-{(S)-2-[2-(tert-Butylsulfonylmethyl)thiazol-4-yl]-2-[(S)-2-(methoxycarbonylamino)-3-phenylpropanamido]ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.40-7.30 (m, 5H), 7.21-7.10 (m, 4H), 7.02 (s,1H), 5.37 (t, 1H, J=6.9 Hz), 5.01-4.98 (m, 2H), 4.51 (t, 1H, J=7.1 Hz),3.77 (s, 3H), 3.34-2.91 (m, 4H), 1.58 (s, 9H).

4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropionamido]-2-(2-phenylthiazole-4-yl)ethyl}phenylsulfamicacid: ¹H NMR (300 MHz, DMSO-d₆) δ 7.96-7.99 (m, 2H), 7.51-7.56 (m, 3H),7.13-7.38 (m, 6H), 6.92-6.95 (m, 4H), 5.11-5.16 (m, 1H), 4.32-4.35 (m,1H), 3.51 (s, 3H), 3.39-3.40 (m, 2H), 3.09-3.19 (m, 1H), 2.92-3.02 (m,2H), 2.75 (dd, J=10.5 Hz and 9.9 Hz, 1H).

4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.61-7.56 (m, 2H), 7.25-7.01 (m, 10H), 6.75 (s,1H), 5.24-5.21 (q, 1H, J=7.2 Hz), 4.38 (t, 1H, J=7.2 Hz), 3.60 (s, 3H),3.23-3.14 (m, 1H), 3.08-3.00 (m, 2H), 2.87-2.80 (m, 1H).

4-{(S)-2-[2-(3-Chlorothiophen-2-yl)thiazol-4-yl]-2-[(S)-2-(methoxycarbonylamino)-3-phenylpropanamido]ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.78-7.76 (d, 1H, J=5.4 Hz), 7.36-7.14 (m, 10H),7.03 (s, 1H), 5.39 (t, 1H, J=6.9 Hz), 4.54 (t, 1H, J=7.3 Hz), 3.80 (s,3H), 3.39-2.98 (m, 4H).

4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-[2-(3-methylthiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.38 (d, 1H, J=5.1 Hz), 7.15-6.93 (m, 10H), 6.73(s, 1H), 5.17 (t, 1H, J=6.9 Hz), 4.31 (t, 1H, J=7.3 Hz), 3.57 (s, 3H),3.18-3.11 (m, 1H), 3.02-2.94 (m, 2H), 2.80-2.73 (m, 1H), 2.46 (s, 3H).

4-{[(S)-2-(2-(Furan-2-yl)thiazol-4-yl]-2-[(S)-2-(methoxycarbonylamino)-3-phenylpropanamido]ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.54-7.46 (m, 1H), 7.02-6.79 (m, 10H), 6.55-6.51(m, 1H), 6.44-6.41 (m, 1H), 5.02-5.00 (q, 1H, J=6.4 Hz), 4.16-4.14 (q,1H, J=7.1 Hz), 3.43 (s, 3H), 2.96-2.58 (m, 4H).

4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-[2-(2-methylthiazole-4-yl)thiazole-4-yl]ethyl}phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 8.27 (d, J=5.4 Hz, 1H), 7.97 (s, 1H),6.99-7.21 (m, 8H), 5.18-5.30 (m, 1H), 4.30-4.39 (m, 1H), 3.64 (s, 3H),3.20 (dd, J=14.1 and 6.6 Hz, 1H), 2.98-3.08 (m, 2H), 2.84 (dd, J=14.1and 6.6 Hz, 1H), 2.78 (s, 3H).

4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-[(2-pyrazin-2-yl)thiazole-4-yl]ethyl}phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 9.34 (s, 1H), 8.65 (s, 2H), 8.34 (d,J=8.1 Hz, 1H), 7.00-5.16 (m. 9H), 5.30 (q, J=7.2 Hz, 1H), 4.41 (t, J=7.2Hz, 1H), 3.65 (s, 3H), 3.23 (dd, J=13.8 and 6.9 Hz, 1H), 2.98-3.13 (m,2H), 2.85 (dd, J=13.8 and 6.9 Hz, 1H).

4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-[2-(6-methylpyridin-3-yl)thiazol-4-yl]ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD): δ 8.90 (s, 1H), 8.19-8.13 (m, 1H), 7.39-7.36 (d,1H, J=8.2 Hz), 7.07-6.88 (m, 9H), 6.79 (s, 1H), 5.17 (t, 1H, J=7.0 Hz),4.29 (t, 1H, J=7.4 Hz), 3.54 (s, 3H), 3.10-2.73 (m, 4H), 2.53 (s, 3H).

Category III of the present disclosure relates to compounds wherein R isa substituted or unsubstituted thiazol-2-yl unit having the formula:

one embodiment of which relates to inhibitors having the formula:

wherein R units are thiazol-2-yl units, that when substituted, aresubstituted with R² and R³ units. R and R^(5a) units are furtherdescribed in Table V.

TABLE V No. R R^(5a) E101 thiazol-2-yl (S)-benzyl E1024-methylthiazol-2-yl (S)-benzyl E103 4-ethylthiazol-2-yl (S)-benzyl E1044-propylthiazol-2-yl (S)-benzyl E105 4-iso-propylthiazol-2-yl (S)-benzylE106 4-cyclopropylthiazol-2-yl (S)-benzyl E107 4-butylthiazol-2-yl(S)-benzyl E108 4-tert-butylthiazol-2-yl (S)-benzyl E1094-cyclohexylthiazol-2-yl (S)-benzyl E1104-(2,2,2-trifluoroethyl)thiazol-2-yl (S)-benzyl E1114-(3,3,3-trifluoropropyl)thiazol-2-yl (S)-benzyl E1124-(2,2-difluorocyclopropyl)thiazol-2-yl (S)-benzyl E1134-(methoxymethyl)thiazol-2-yl (S)-benzyl E114 4-(carboxylic acid ethylester)thiazol-2-yl (S)-benzyl E115 4,5-dimethylthiazol-2-yl (S)-benzylE116 4-methyl-5-ethylthiazol-2-yl (S)-benzyl E117 4-phenylthiazol-2-yl(S)-benzyl E118 4-(4-chlorophenyl)thiazol-2-yl (S)-benzyl E1194-(3,4-dimethylphenyl)thiazol-2-yl (S)-benzyl E1204-methyl-5-phenylthiazol-2-yl (S)-benzyl E1214-(thiophen-2-yl)thiazol-2-yl (S)-benzyl E1224-(thiophen-3-yl)thiazol-2-yl (S)-benzyl E1234-(5-chlorothiophen-2-yl)thiazol-2-yl (S)-benzyl E1245,6-dihydro-4H-cyclopenta[(d]thiazol-2-yl (S)-benzyl E1254,5,6,7-tetrahydrobenzo[d]thiazol-2-yl (S)-benzyl

The compounds encompassed within Category III of the present disclosurecan be prepared by the procedure outlined in Scheme IV and described inExample 5 herein below.

Example 54-[(S)-2-(S)-2-Acetamido-3-phenylpropanamido)-2-(4-ethylthiazol-2-yl)ethyl]phenylsulfamicacid (15)

Preparation of(S)-2-acetamido-N—[(S)-1-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)-ethyl]-3-phenylpropanamide(14): To a solution of1-(S)-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethyl amine hydrobromide,3, (0.343 g, 0.957 mmol), N-acetyl-L-phenylalanine (0.218 g),1-hydroxybenzotriazole (HOBt) (0.161 g), diisopropyl-ethylamine (0.26g), in DMF (10 mL) at 0°, is added1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDCI) (0.201 g). Themixture is stirred at 0° C. for 30 minutes then at room temperatureovernight. The reaction mixture is diluted with water and extracted withEtOAc. The combined organic phase is washed with 1 N aqueous HCl, 5%aqueous NaHCO₃, water and brine, and dried over Na₂SO₄. The solvent isremoved in vacuo to afford 0.313 g (70% yield) of the desired productwhich is used without further purification. LC/MS ESI+467 (M+1).

Preparation of4-((S)-2-(S)-2-acetamido-3-phenylpropanamido)-2-(4-ethylthiazol-2-yl)ethyl)phenylsulfamicacid (15):(S)-2-Acetamido-N—[(S)-1-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethyl]-3-phenylpropanamide,14, (0.313 g) is dissolved in MeOH (4 mL). A catalytic amount of Pd/C(10% w/w) is added and the mixture is stirred under a hydrogenatmosphere 2 hours. The reaction mixture is filtered through a bed ofCELITE™ and the solvent is removed under reduced pressure. The crudeproduct is dissolved in pyridine (12 mL) and treated with SO₃-pyridine(0.320 g). The reaction is stirred at room temperature for 5 minutesafter which a 7% solution of NH₄OH (30 mL) is added. The mixture is thenconcentrated and the resulting residue is purified by reverse phasechromatography to afford 0.215 g of the desired product as the ammoniumsalt. ¹H NMR (CD₃OD): δ 7.23-6.98 (m, 10H), 5.37 (t, 1H), 4.64 (t, 1H,J=6.3 Hz), 3.26-2.74 (m, 6H), 1.91 (s, 3H), 1.29 (t, 3H, J=7.5 Hz).

The following are further non-limiting examples of compounds encompassedwithin Category III of the present disclosure.

4-[(S)-2-((S)-2-Acetamido-3-phenylpropanamido)-2-(4-tert-butylthiazol-2-yl)ethyl]phenylsulfamicacid: ¹H NMR (300 MHz, CD₃OD): δ 7.22-7.17 (m, 5H), 7.06 (dd, J=14.1,8.4 Hz, 4H), 6.97 (d, J=0.9 Hz, 1H), 5.39 (dd, J=8.4, 6.0 Hz, 1H), 4.65(t, J=7.2 Hz, 1H), 3.33-3.26 (m, 1H), 3.13-3.00 (m, 3H), 2.80 (dd,J=13.5, 8.7 Hz, 1H), 1.91 (s, 3H), 1.36 (s, 9H).

4-{(S)-2-(S)-2-Acetamido-3-phenylpropanamido)-2-[4-(thiophen-3-yl)thiazol-2-yl]ethyl)phenylsulfamicacid: ¹H NMR (300 MHz, CD₃OD): δ 8.58 (d, J=8.1 Hz, 1H), 7.83-7.82 (m,1H), 7.57-7.46 (m, 3H), 7.28-6.93 (m, 11H), 5.54-5.43 (m, 1H), 4.69-4.55(m, 2H), 3.41-3.33 (m, 1H), 3.14-3.06 (3H), 2.86-2.79 (m, 1H), 1.93 (s,3H).

The first aspect of Category IV of the present disclosure relates tocompounds wherein R is a substituted or unsubstituted thiazol-2-yl unithaving the formula:

one embodiment of which relates to inhibitors having the formula:

wherein R units and R^(5a) units further described in Table VI.

TABLE VI No. R R^(5a) F126 thiazol-2-yl hydrogen F1274-methylthiazol-2-yl hydrogen F128 4-ethylthiazol-2-yl hydrogen F1294-propylthiazol-2-yl hydrogen F130 4-iso-propylthiazol-2-yl hydrogenF131 4-cyclopropylthiazol-2-yl hydrogen F132 4-butylthiazol-2-ylhydrogen F133 4-tert-butylthiazol-2-yl hydrogen F1344-cyclohexylthiazol-2-yl hydrogen F135 4,5-dimethylthiazol-2-yl hydrogenF136 4-methyl-5-ethylthiazol-2-yl hydrogen F137 4-phenylthiazol-2-ylhydrogen F138 thiazol-2-yl (S)-iso-propyl F139 4-methylthiazol-2-yl(S)-iso-propyl F140 4-ethylthiazol-2-yl (S)-iso-propyl F1414-propylthiazol-2-yl (S)-iso-propyl F142 4-iso-propylthiazol-2-yl(S)-iso-propyl F143 4-cyclopropylthiazol-2-yl (S)-iso-propyl F1444-butylthiazol-2-yl (S)-iso-propyl F145 4-tert-butylthiazol-2-yl(S)-iso-propyl F146 4-cyclohexylthiazol-2-yl (S)-iso-propyl F1474,5-dimethylthiazol-2-yl (S)-iso-propyl F1484-methyl-5-ethylthiazol-2-yl (S)-iso-propyl F149 4-phenylthiazol-2-yl(S)-iso-propyl F150 4-(thiophen-2-yl)thiazol-2-yl (S)-iso-propyl

The compounds encompassed within Category IV of the present disclosurecan be prepared by the procedure outlined in Scheme V and described inExample 6 herein below.

Example 64-{(S)-2-[(S)-2-(tert-Butoxycarbonylamino)-3-methylbutanamido]-2-(4-ethylthiazol-2-yl)ethyl}phenylsulfamicacid (17)

Preparation of tert-butyl(S)-1-[(S)-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethylamino]-3-methyl-1-oxobutan-2-ylcarbamate(16): To a solution of1-(S)-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethyl amine hydrobromide,3, (0.200 g, 0.558 mmol),(S)-(2-tert-butoxycarbonylamino)-3-methylbutyric acid (0.133 g) and1-hydroxybenzotriazole (HOBt) (0.094 g) in DMF (5 mL) at 0°, is added1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDCI) (0.118 g) followedby diisopropylamine (0.151 g). The mixture is stirred at 0° C. for 30minutes then at room temperature overnight. The reaction mixture isdiluted with water and extracted with EtOAc. The combined organic phaseis washed with 1 N aqueous HCl, 5% aqueous NaHCO₃, water and brine, anddried over Na₂SO₄. The solvent is removed in vacuo to afford 0.219 g(82% yield) of the desired product which is used without furtherpurification. LC/MS ESI+477 (M+1).

Preparation of4-{(S)-2-[(S)-2-(tert-butoxycarbonylamino)-3-methylbutanamido]-2-(4-ethylthiazol-2-yl)ethyl}phenylsulfamicacid (17): tert-Butyl(S)-1-[(S)-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethylamino]-3-methyl-1-oxobutan-2-ylcarbamate,16, (0.219 g) is dissolved in MeOH (4 mL). A catalytic amount of Pd/C(10% w/w) is added and the mixture is stirred under a hydrogenatmosphere 2 hours. The reaction mixture is filtered through a bed ofCELITE™ and the solvent is removed under reduced pressure. The crudeproduct is dissolved in pyridine (5 mL) and treated with SO₃-pyridine(0.146 g). The reaction is stirred at room temperature for 5 minutesafter which a 7% solution of NH₄OH (30 mL) is added. The mixture is thenconcentrated and the resulting residue is purified by reverse phasechromatography to afford 0.148 g of the desired product as the ammoniumsalt. ¹H NMR (CD₃OD): δ 7.08 (s, 4H), 7.02 (s, 1H), 5.43 (s, 1H), 3.85(s, 1H), 3.28-2.77 (m, 4H), 1.94 (s, 1H), 1.46 (s, 9H), 1.29 (s, 3H,J=7.3 Hz), 0.83 (s, 6H).

The following are further non-limiting examples of the second aspect ofCategory IV of the present disclosure.

(S)-4-{2-[2-(tert-Butoxycarbonyl)acetamide]-2-(4-ethylthiazol-2-yl)ethyl}-phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.09-6.91 (m, 5H), 5.30 (t, 1H, J=8.4 Hz),3.60-2.64 (m, 6H), 1.34 (s, 9H), 1.16 (t, 3H, J=7.5 Hz).

4-{(S)-2-[(S)-2-(tert-Butoxycarbonylamino)-4-methylpentanamido]-2-(4-ethylthiazol-2-yl)ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD) δ 7.19-7.00 (m, 4H), 5.50-5.40 (m, 1H), 4.13-4.06(m, 1H), 3.32 (1H, A of ABX, J=7.5, 18 Hz), 3.12 (1H, B of ABX, J=8.1,13.8 Hz), 2.79 (q, 2H, J=7.8, 14.7 Hz), 1.70-1.55 (m, 1H), 1.46 (s, 9H),1.33 (t, 3H, J=2.7 Hz), 0.92 (q, 6H, J=6, 10.8 Hz).

4-{(S)-2-[(S)-2-(tert-Butoxycarbonylamino)-4-methylpentanamido]-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD) δ 8.06 (d, 1H, J=8.4 Hz), 7.61-7.58 (m, 1H), 7.57(s, 1H), 7.15 (t, 1H, J=0.6 Hz), 7.09-6.98 (m, 6H), 5.30-5.20 (m, 1H),4.10-4.00 (m, 1H), 3.19-3.13 (m, 2H), 1.63-1.55 (m, 2H), 1.48-1.33 (m,10H), 0.95-0.89 (m, 6H).

(S)-4-{2-[2-(tert-Butoxycarbonyl)acetamide]-2-(4-ethylthiazol-2-yl)ethyl}-phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.09-6.91 (m, 5H), 5.30 (t, 1H, J=8.4 Hz),3.60-2.64 (m, 6H), 1.34 (s, 9H), 1.16 (t, 3H, J=7.5 Hz).

A further embodiment of Category IV relates to inhibitors having theformula:

wherein R units and R^(5a) units further described in Table VII.

TABLE VII No. R R^(5a) G151 thiazol-2-yl hydrogen G1524-methylthiazol-2-yl hydrogen G153 4-ethylthiazol-2-yl hydrogen G1544-propylthiazol-2-yl hydrogen G155 4-iso-propylthiazol-2-yl hydrogenG156 4-cyclopropylthiazol-2-yl hydrogen G157 4-butylthiazol-2-ylhydrogen G158 4-tert-butylthiazol-2-yl hydrogen G1594-cyclohexylthiazol-2-yl hydrogen G160 4,5-dimethylthiazol-2-yl hydrogenG161 4-methyl-5-ethylthiazol-2-yl hydrogen G162 4-phenylthiazol-2-ylhydrogen G163 thiazol-2-yl (S)-iso-propyl G164 4-methylthiazol-2-yl(S)-iso-propyl G165 4-ethylthiazol-2-yl (S)-iso-propyl G1664-propylthiazol-2-yl (S)-iso-propyl G167 4-iso-propylthiazol-2-yl(S)-iso-propyl G168 4-cyclopropylthiazol-2-yl (S)-iso-propyl G1694-butylthiazol-2-yl (S)-iso-propyl G170 4-tert-butylthiazol-2-yl(S)-iso-propyl G171 4-cyclohexylthiazol-2-yl (S)-iso-propyl G1724,5-dimethylthiazol-2-yl (S)-iso-propyl G1734-methyl-5-ethylthiazol-2-yl (S)-iso-propyl G174 4-phenylthiazol-2-yl(S)-iso-propyl G175 4-(thiophen-2-yl)thiazol-2-yl (S)-iso-propyl

The compounds encompassed within this embodiment of Category IV can bemade according to the procedure outlined in Scheme V and described inExample 6 by substituting the corresponding methylcarbamate for theBoc-protected reagent. The following are non-limiting examples of thisembodiment.

4-{(S)-2-(4-Ethylthiazol-2-yl)-2-[(S)-2-(methoxycarbonyl)-4-methylpentan-amido]ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD) δ 7.12-7.03 (m, 5H), 6.84 (d, 1H, J=8.4 Hz), 5.40(t, 1H, J=5.7 Hz), 4.16 (t, 1H, J=6.3 Hz), 3.69 (s, 3H), 3.61-3.55 (m,1H), 3.29-3.27 (m, 1H), 3.14-3.07 (m, 1H), 2.81 (q, 2H, J=3.9, 11.2 Hz),1.66-1.59 (m, 1H), 1.48-1.43 (m, 2H), 1.31 (t, 3H, J=4.5 Hz), 0.96-0.90(m, 6H).

(S)-4-{2-(4-Ethylthiazol-2-yl)-2-[2-(methoxycarbonyl)acetamido]ethyl}phenyl-sulfamicacid: ¹H NMR (CD₃OD): δ 7.12-7.07 (m, 4H), 7.03 (s, 1H), 5.42 (t, 1H,J=5.7 Hz), 3.83-3.68 (q, 2H, J=11.4 Hz), 3.68 (s, 3H), 3.34-3.04 (m,2H), 2.83-2.76 (q, 2H, J=7.8 Hz), 1.31 (t, 3H, J=7.5 Hz).

4-{(S)-2-(4-Ethylthiazol-2-yl)-2-[(S)-2-(methoxycarbonyl)-3-methylbutanamido]-ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD) δ 8.56 (d, 1H, J=7.8 Hz), 7.09 (s, 4H), 7.03 (s,1H), 5.26-5.20 (m, 1H), 3.90 (d, 1H, J=7.8 Hz), 3.70 (s, 3H), 3.30 (1H,A of ABX, obscured by solvent), 3.08 (1H, B of ABX, J=9.9, 9 Hz), 2.79(q, 2H, J=11.1, 7.2 Hz), 2.05-1.97 (m, 1H), 1.31 (t, 3H, J=7.5 Hz), 0.88(s, 3H), 0.85 (s, 3H), 0.79-0.75 (m, 1H).

4-{(S)-2-[(S)-2-(Methoxycarbonyl)-4-methylpentanamido]-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD) δ 8.22 (d, 1H, J=9 Hz), 7.62-7.57 (m, H), 7.15 (t,1H, J=0.6 Hz), 7.10-6.97 (m, 4H), 5.30-5.20 (m, 1H), 4.16-4.11 (m, 1H),3.67 (s, 2H), 3.22 (1H, A of ABX, J=6.9, 13.5 Hz), 3.11 (1H, B of ABX,J=7.8, 13.6 Hz), 1.65-1.58 (m, 1H), 1.50-1.45 (m, 2H), 0.95-0.88 (m,6H).

Category IV of the present disclosure relates to compounds having theformula:

wherein R is a substituted or unsubstituted thiophen-2-yl orthiophen-4-yl unit and non-limiting examples of R² are further describedin Table VIII.

TABLE VIII No. R R⁸ H176 thiazol-2-yl —OC(CH₃)₃ H1774-methylthiazol-2-yl —OC(CH₃)₃ H178 4-ethylthiazol-2-yl —OC(CH₃)₃ H1794-cyclopropylthiazol-2-yl —OC(CH₃)₃ H180 4-tert-butylthiazol-2-yl—OC(CH₃)₃ H181 4-cyclohexylthiazol-2-yl —OC(CH₃)₃ H1824-(2,2,2-trifluoroethyl)thiazol-2-yl —OC(CH₃)₃ H1834-(3,3,3-trifluoropropyl)thiazol-2-yl —OC(CH₃)₃ H1844-(2,2-difluorocyclopropyl)thiazol-2-yl —OC(CH₃)₃ H1854,5-dimethylthiazol-2-yl —OC(CH₃)₃ H186 4-methyl-5-ethylthiazol-2-yl—OC(CH₃)₃ H187 4-phenylthiazol-2-yl —OC(CH₃)₃ H1884-(4-chlorophenyl)thiazol-2-yl —OC(CH₃)₃ H1894-(3,4-dimethylphenyl)thiazol-2-yl —OC(CH₃)₃ H1904-methyl-5-phenylthiazol-2-yl —OC(CH₃)₃ H1914-(thiophen-2-yl)thiazol-2-yl —OC(CH₃)₃ H192 thiazol-4-yl —OC(CH₃)₃ H1934-methylthiazol-4-yl —OC(CH₃)₃ H194 4-ethylthiazol-4-yl —OC(CH₃)₃ H1954-cyclopropylthiazol-4-yl —OC(CH₃)₃ H196 4-tert-butylthiazol-4-yl—OC(CH₃)₃ H197 4-cyclohexylthiazol-4-yl —OC(CH₃)₃ H1984-(2,2,2-trifluoroethyl)thiazol-4-yl —OC(CH₃)₃ H1994-(3,3,3-trifluoropropyl)thiazol-4-yl —OC(CH₃)₃ H2004-(2,2-difluorocyclopropyl)thiazol-4-yl —OC(CH₃)₃ H2014,5-dimethylthiazol-4-yl —OC(CH₃)₃ H202 4-methyl-5-ethylthiazol-4-yl—OC(CH₃)₃ H203 4-phenylthiazol-4-yl —OC(CH₃)₃ H2044-(4-chlorophenyl)thiazol-4-yl —OC(CH₃)₃ H2054-(3,4-dimethylphenyl)thiazol-4-yl —OC(CH₃)₃ H2064-methyl-5-phenylthiazol-4-yl —OC(CH₃)₃ H2074-(thiophen-2-yl)thiazol-4-yl —OC(CH₃)₃ H208 thiazol-2-yl —OCH₃ H2094-methylthiazol-2-yl —OCH₃ H210 4-ethylthiazol-2-yl —OCH₃ H2114-cyclopropylthiazol-2-yl —OCH₃ H212 4-tert-butylthiazol-2-yl —OCH₃ H2134-cyclohexylthiazol-2-yl —OCH₃ H214 4-(2,2,2-trifluoroethyl)thiazol-2-yl—OCH₃ H215 4-(3,3,3-trifluoropropyl)thiazol-2-yl —OCH₃ H2164-(2,2-difluorocyclopropyl)thiazol-2-yl —OCH₃ H2174,5-dimethylthiazol-2-yl —OCH₃ H218 4-methyl-5-ethylthiazol-2-yl —OCH₃H219 4-phenylthiazol-2-yl —OCH₃ H220 4-(4-chlorophenyl)thiazol-2-yl—OCH₃ H221 4-(3,4-dimethylphenyl)thiazol-2-yl —OCH₃ H2224-methyl-5-phenylthiazol-2-yl —OCH₃ H223 4-(thiophen-2-yl)thiazol-2-yl—OCH₃ H224 thiazol-4-yl —OCH₃ H225 4-methylthiazol-4-yl —OCH₃ H2264-ethylthiazol-4-yl —OCH₃ H227 4-cyclopropylthiazol-4-yl —OCH₃ H2284-tert-butylthiazol-4-yl —OCH₃ H229 4-cyclohexylthiazol-4-yl —OCH₃ H2304-(2,2,2-trifluoroethyl)thiazol-4-yl —OCH₃ H2314-(3,3,3-trifluoropropyl)thiazol-4-yl —OCH₃ H2324-(2,2-difluorocyclopropyl)thiazol-4-yl —OCH₃ H2334,5-dimethylthiazol-4-yl —OCH₃ H234 4-methyl-5-ethylthiazol-4-yl —OCH₃H235 4-phenylthiazol-4-yl —OCH₃ H236 4-(4-chlorophenyl)thiazol-4-yl—OCH₃ H237 4-(3,4-dimethylphenyl)thiazol-4-yl —OCH₃ H2384-methyl-5-phenylthiazol-4-yl —OCH₃ H239 4-(thiophen-2-yl)thiazol-4-yl—OCH₃ H240 thiazol-2-yl —CH₃ H241 4-methylthiazol-2-yl —CH₃ H2424-ethylthiazol-2-yl —CH₃ H243 4-cyclopropylthiazol-2-yl —CH₃ H2444-tert-butylthiazol-2-yl —CH₃ H245 4-cyclohexylthiazol-2-yl —CH₃ H2464-(2,2,2-trifluoroethyl)thiazol-2-yl —CH₃ H2474-(3,3,3-trifluoropropyl)thiazol-2-yl —CH₃ H2484-(2,2-difluorocyclopropyl)thiazol-2-yl —CH₃ H2494,5-dimethylthiazol-2-yl —CH₃ H250 4-methyl-5-ethylthiazol-2-yl —CH₃H251 4-phenylthiazol-2-yl —CH₃ H252 4-(4-chlorophenyl)thiazol-2-yl —CH₃H253 4-(3,4-dimethylphenyl)thiazol-2-yl —CH₃ H2544-methyl-5-phenylthiazol-2-yl —CH₃ H255 4-(thiophen-2-yl)thiazol-2-yl—CH₃ H256 thiazol-4-yl —CH₃ H257 4-methylthiazol-4-yl —CH₃ H2584-ethylthiazol-4-yl —CH₃ H259 4-cyclopropylthiazol-4-yl —CH₃ H2604-tert-butylthiazol-4-yl —CH₃ H261 4-cyclohexylthiazol-4-yl —CH₃ H2624-(2,2,2-trifluoroethyl)thiazol-4-yl —CH₃ H2634-(3,3,3-trifluoropropyl)thiazol-4-yl —CH₃ H2644-(2,2-difluorocyclopropyl)thiazol-4-yl —CH₃ H2654,5-dimethylthiazol-4-yl —CH₃ H266 4-methyl-5-ethylthiazol-4-yl —CH₃H267 4-phenylthiazol-4-yl —CH₃ H268 4-(4-chlorophenyl)thiazol-4-yl —CH₃H269 4-(3,4-dimethylphenyl)thiazol-4-yl —CH₃ H2704-methyl-5-phenylthiazol-4-yl —CH₃ H271 4-(thiophen-2-yl)thiazol-4-yl—CH₃

The compounds encompassed within Category IV of the present disclosurecan be prepared by the procedure outlined in VI and described in Example7 herein below.

Example 7[1-(S)-(Phenylthiazol-2-yl)-2-(4-sulfoaminophenyl)ethyl]-carbamic acidtert-butyl ester (19)

Preparation of[2-(4-nitrophenyl)-1-(S)-(4-phenylthiazol-2-yl)ethyl]-carbamic acidtert-butyl ester (18): A mixture of[2-(4-nitrophenyl)-1-(S)-thiocarbamoylethyl]-carbamic acid tert-butylester, 2, (0.343 g, 1.05 mmol), 2-bromoacetophenone (0.231 g, 1.15mmol), in CH₃CN (5 mL) is refluxed 1.5 hour. The solvent is removedunder reduced pressure and the residue re-dissolved in CH₂Cl₂ thenpyridine (0.24 mL, 3.0 mmol) and Boc₂O (0.24 mL, 1.1 mmol) are added.The reaction is stirred for 2 hours and diethyl ether is added to thesolution and the precipitate which forms is removed by filtration. Theorganic layer is dried (Na₂SO₄), filtered, and concentrated to a residuewhich is purified over silica to afford 0.176 g (39%) of the desiredproduct ESI+MS 426 (M+1).

Preparation of[1-(S)-(phenylthiazol-2-yl)-2-(4-sulfoaminophenyl)ethyl]-carbamic acidtert-butyl ester (19):[2-(4-nitrophenyl)-1-(S)-(4-phenylthiazol-2-yl)ethyl]-carbamic acidtert-butyl ester, 18, (0.176 g, 0.41 mmol) is dissolved in MeOH (4 mL).A catalytic amount of Pd/C (10% w/w) is added and the mixture is stirredunder a hydrogen atmosphere 12 hours. The reaction mixture is filteredthrough a bed of CELITE™ and the solvent is removed under reducedpressure. The crude product is dissolved in pyridine (12 mL) and treatedwith SO₃-pyridine (0.195 g, 1.23 mmol). The reaction is stirred at roomtemperature for 5 minutes after which a 7% solution of NH₄OH (10 mL) isadded. The mixture is then concentrated and the resulting residue ispurified by reverse phase chromatography to afford 0.080 g of thedesired product as the ammonium salt. ¹H NMR (300 MHz, MeOH-d₄) δ 7.93(d, J=6.0 Hz, 2H), 7.68 (s, 1H), 7.46-7.42 (m, 3H), 7.37-7.32 (m, 1H),7.14-7.18 (m, 3H), 5.13-5.18 (m, 1H), 3.40 (dd, J=4.5 and 15.0 Hz, 1H),3.04 (dd, J=9.6 and 14.1 Hz, 1H), 1.43 (s, 9H).

The following are further non-limiting examples of Category IV of thepresent disclosure.

(S)-4-(2-(4-Methylthiazol-2-yl)-2-pivalamidoethyl)phenylsulfamic acid:¹H NMR (CD₃OD): δ 7.31 (s, 4H), 7.20 (s, 1H), 5.61-5.56 (m, 1H),3.57-3.22 (m, 2H), 2.62 (s, 3H), 1.31 (s, 3H).

(S)-4-(2-(4-Ethylthiazol-2-yl)-2-pivalamidoethyl)phenylsulfamic acid: ¹HNMR (300 MHz, MeOH-d₄) δ 7.92 (d, J=8.1 Hz, 1H), 7.12-7.14 (m, 4H), 7.03(s, 1H), 5.38-5.46 (m, 1H), 3.3-3.4 (m, 1H), 3.08 (dd, J=10.2 and 13.8Hz, 1H), 2.79 (q, J=7.2 Hz, 2H), 1.30 (t, J=7.2 Hz, 3H), 1.13 (s, 9H).

(S)-4-(2-(4-(Hydroxymethyl)thiazol-2-yl)-2-pivalamidoethyl)phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.92 (d, J=8.1 Hz, 1H), 7.24 (s, 1H),7.08 (d, J=8.7 Hz, 2H), 7.00 (d, J=8.7 Hz, 2H), 5.29-5.37 (m, 1H), 4.55(s, 2H), 3.30 (dd, J=4.8 and 13.5 Hz, 1H), 2.99 (dd, J=10.5 and 13.5 Hz,1H), 0.93 (s, 9H).

(S)-4-(2-(4-(Ethoxycarbonyl)thiazol-2-yl)-2-pivalamidoethyl)phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 8.30 (s, 1H), 8.04 (d, J=8.1 Hz, 1H),7.13 (s, 4H), 5.41-5.49 (m, 1H), 4.41 (q, J=7.2 Hz, 2H), 3.43 (dd, J=5.1and 13.8 Hz, 1H), 3.14 (dd, J=5.7 and 9.9 Hz, 1H), 1.42 (t, J=7.2 Hz,3H), 1.14 (s, 9H).

(S)-4-(2-(4-Phenylthiazol-2-yl)-2-pivalamidoethyl)phenylsulfamic acid:¹H NMR (300 MHz, MeOH-d₄) δ 7.94-8.01 (m, 3H), 7.70 (s, 1H), 7.42-7.47(m, 2H), 7.32-7.47 (m, 1H), 7.13-7.20 (m, 3H), 5.48-5.55 (m, 1H), 3.50(dd, J=5.1 and 14.1 Hz, 1H), 3.18 (dd, J=10.2 and 14.1 Hz, 1H), 1.17 (s,9H).

4-((S)-2-(4-(3-Methoxyphenyl)thiazol-2-yl)-2-pivalamidoethyl)phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.96-7.93 (d, 1H, J=8.1 Hz), 7.69 (s, 1H),7.51-7.49 (d, 2H, J=7.9 Hz), 7.33 (t, 1H, J=8.0 Hz), 7.14 (s, 4H),6.92-6.90 (d, 1H, J=7.8 Hz), 5.50 (t, 1H, J=5.1 Hz), 3.87 (s, 3H),3.50-3.13 (m, 2H), 1.15 (s, 9H).

4-((S)-2-(4-(2,4-Dimethoxyphenyl)thiazol-2-yl)-2-pivalamidoethyl)phenylsulfamicacid: ¹H NMR (CD₃OD): δ 8.11-8.09 (d, 1H, J=7.8 Hz), 7.96-7.93 (d, 1H,J=8.4 Hz), 7.74 (s, 1H), 7.18-7.16 (m, 4H), 6.67-6.64 (d, 2H, J=9.0 Hz),5.55-5.47 (m, 1H), 3.95 (s, 3H), 3.87 (s, 3H), 3.52-3.13 (m, 2H), 1.17(s, 9H).

(S)-4-(2-(4-Benzylthiazol-2-yl)-2-pivalamidoethyl)phenylsulfamic acid ¹HNMR (CD₃OD) δ 7.85 (d, 1H, J=8.4 Hz), 7.38-7.20 (m, 4H), 7.11-7.02 (m,1H), 7.00 (s, 1H), 5.42-5.37 (m, 1H), 4.13 (s, 2H), 3.13-3.08 (m, 2H),1.13 (s, 9H).

(S)-4-(2-Pivalamido-2-(4-(thiophen-2-ylmethyl)thiazol-2-yl)ethyl)phenylsulfamicacid: ¹H NMR (CD₃OD) δ 7.88-7.85 (d, 1H), 7.38-7.35 (m, 1H), 7.10-7.01(m, 4H), 7.02 (s, 1H), 5.45-5.38 (m, 1H), 4.13 (s, 2H), 3.13-3.05 (m,2H), 1.13 (2, 9H).

(S)-4-(2-(4-(3-Methoxybenzyl)thiazol-2-yl)-2-pivalamidoethyl)phenylsulfamicacid: ¹H NMR (CD₃OD) δ 7.85 (d, 1H, J=8.4 Hz), 7.25-7.20 (m, 1H),7.11-7.02 (m, 4H), 7.01 (s, 1H), 6.90-6.79 (m, 2H), 5.45-5.40 (m, 1H),4.09 (s, 2H), 3.79 (s, 3H), 3.12-3.08 (m, 2H), 1.10 (s, 9H).

4-((S)-2-(4-(2,3-Dihydrobenzo[b][1,4]dioxin-6-yl)thiazol-2-yl)-2-pivalamidoethyl)-phenylsulfamicacid: ¹H(CD₃OD): δ 7.53 (s, 1H), 7.45 (s, 1H), 7.42-7.40 (d, 1H, J=8.4Hz), 7.19-7.15 (m, 4H), 6.91-6.88 (d, 2H, J=8.4 Hz), 5.51-5.46 (m, 1H),4.30 (s, 4H), 3.51-3.12 (m, 2H), 1.16 (s, 9H).

(S)-4-(2-(5-Methyl-4-phenylthiazol-2-yl)-2-pivalamidoethyl)phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.63-7.60 (d, 2H, J=7.1 Hz), 7.49-7.35 (m, 3H),7.14 (s, 4H), 5.43-5.38 (m, 1H), 3.42-3.09 (m, 2H), 2.49 (s, 3H), 1.14(s, 9H).

(S)-4-(2-(4-(Biphen-4-yl)thiazol-2-yl)-2-pivalamidoethyl)phenylsulfamicacid: ¹H NMR (CD₃OD): δ 8.04-8.01 (m, 2H), 7.72-7.66 (m, 5H), 7.48-7.35(m, 3H), 7.15 (s, 4H), 5.50 (t, 1H, J=5.0 Hz), 3.57-3.15 (d, 2H), 1.16(s, 9H).

(S)-4-(2-tert-Butoxycarbonyl-2-(2-methylthaizol-4-yl)-phenylsulfamicacid ¹H NMR (300 MHz, D₂O) δ 6.99-7.002 (m, 4H), 6.82 (s, 1H), 2.26 (dd,J=13.8 and 7.2 Hz, 1H), 2.76 (dd, J=13.8 and 7.2 Hz, 1H), 2.48 (s, 3H),1.17 (s, 9H).

(S)-4-(2-(tert-Butoxycarbonyl)-2-(4-propylthiazol-2-yl)ethyl)-phenylsulfamic acid: ¹H NMR (300 MHz, CD₃OD): δ 7.18-7.02 (m, 5H), 5.06-5.03(m, 1H), 3.26 (dd, J=13.8, 4.8 Hz, 1H), 2.95 (dd, J=13.8, 9.3 Hz, 1H),2.74 (dd, J=15.0, 7.2 Hz, 2H), 1.81-1.71 (m, 2H), 1.40 (s, 7H), 1.33(bs, 2H), 0.988 (t, J=7.5 Hz 3H).

(S)-4-(2-(tert-Butoxycarbonyl)-2-(4-tert-butylthiazol-2-yl)ethyl)-phenylsulfamic acid: ¹H NMR (300 MHz, CD₃OD): δ 7.12 (s, 4H), 7.01 (s, 1H),5.11-5.06 (m, 1H), 3.32-3.25 (m, 1H), 2.96 (m, 1H), 1.42 (s, 8H), 1.38(s, 9H), 1.32 (s, 1H).

(S)-4-(2-(tert-Butoxycarbonylamino)-2-(4-(methoxymethyl)thiazol-2-yl)ethyl)-phenylsulfamic acid: ¹H NMR (300 MHz, CD₃OD): δ 7.36 (s, 1H), 7.14-7.05 (m,4H), 5.06 (dd, J=9.0, 5.1 Hz, 1H), 4.55 (s, 2H), 3.42 (s, 3H), 3.31-3.24(m, 1H), 2.97 (dd, J=13.8, 9.9 Hz, 1H), 1.47-1.31 (m, 9H).

(S)-4-(2-tert-Butoxycarbonylamino)-2-(4-(2-hydroxymethyl)thiazol-2yl)ethyl)phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.22-7.25 (m, 1H), 7.09-7.15 (m, 4H),5.00-5.09 (m, 1H), 4.32-4.35 (m, 1H), 3.87 (t, J=6.6 Hz, 2H), 3.23-3.29(m, 1H), 3.09-3.18 (m, 1H), 2.98 (t, J=6.6 Hz, 2H), 1.41 (s, 9H).

(S)-4-(2-tert-Butoxycarbonylamino)-2-(4-(2-ethoxy-2-oxoethyl)-thiazole-2-yl)-ethyl)phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.29 (s, 1H), 7.09-7.16 (m, 4H),5.04-5.09 (m, 1H), 4.20 (q, J=6.9 Hz, 2H), 3.84 (s, 2H), 3.30 (dd, J=4.8and 14.1 HZ, 1H), 2.97 (dd, J=9.6 Hz and 13.8 Hz, 1H), 1.41 (s, 9H),1.29 (t, J=7.2 Hz, 3H).

(S)-4-(2-(tert-Butoxycarbonylamino)-2-(4-(2-methoxy-2-oxoethyl)thiazol-2-yl)ethyl)phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.31 (s, 1H), 7.01-7.16 (m, 4H),5.04-5.09 (m, 1H), 4.01 (s, 2H), 3.78 (s, 2H), 3.74 (s, 3H), 3.29 (dd,J=5.1 and 13.8 Hz, 1H), 2.99 (dd, J=9.3 and 13.8 Hz, 1H), 1.41 (s, 9H).

(S)-4-(2-(tert-Butoxycarbonylamino)-2-(2-(pivaloyloxy)thiazol-4-yl)ethyl)phenylsulfamicacid: ¹H NMR (300 MHz, D₂O) δ 6.95 (s, 4H), 6.63 (s, 1H), 2.94 (dd,J=13.5 and 4.8 Hz, 1H), 2.75 (dd, J=13.5 and 4.8 Hz, 1H), 1.16 (s, 9H),1.13 (s, 9H).

(S)-4-(2-(tert-Butoxycarbonylamino)-2-(5-phenylthiazol-2-yl)ethyl)-phenylsulfamic acid: ¹H NMR (300 MHz, CD₃OD): δ 7.98 (s, 1H), 7.62 (d, J=7.2Hz, 2H), 7.46-7.35 (m, 4H), 7.14 (s, 4H), 5.09 (bs, 1H), 3.07-2.99 (m,2H), 1.43 (s, 9H).

4-((S)-2-(tert-Butoxycarbonylamino)-2-(4-(3-(trifluoromethyl)phenyl)thiazol-2-yl)ethyl)-phenylsulfamic acid: ¹H NMR (300 MHz, CD₃OD): δ 8.28 (s, 1H), 8.22-8.19 (m,1H), 7.89 (s, 1H), 7.65 (d, J=5.1 Hz, 2H), 7.45 (d, J=8.1 Hz, 1H), 7.15(s, 4H), 5.17-5.14 (m, 1H), 3.43-3.32 (m, 1H), 3.05 (dd, J=14.1, 9.6 Hz,1H), 1.42 (s, 9H).

(S)-4-(2-(tert-Butoxycarbonylamino)-2-(4-phenylthiazol-2-yl)ethyl)-phenylsulfamic acid: ¹H NMR (300 MHz, CD₃OD): δ 7.98 (s, 1H), 7.94 (d, J=7.2Hz, 2H), 7.46-7.35 (m, 4H), 7.14 (s, 4H), 5.09 (bs, 1H), 3.07-2.99 (m,2H), 1.43 (s, 9H).

(S,S)-2-(2-{2-[2-tert-Butoxycarbonylamino-2-(4-sulfoaminophenyl)ethyl]thiazol-4-yl}acetylamido)-3-phenylpropionicacid methyl ester: ¹H NMR (300 MHz, MeOH-d₄) δ 6.85-6.94 (m, 9H), 6.64(s, 1H), 4.83 (s, 1H), 4.54-4.58 (m, 1H), 3.49 (s, 3H), 3.39 (s, 2H),2.80-2.97 (m, 1H), 2.64-2.78 (m, 1H), 1.12 (s, 9H).

(S)-[1-{1-oxo-4-[2-(1-phenyl-1H-tetrazol-5-sulfonypethyl]-1H-1λ⁴-thiazol-2-yl}-2-(4-sulfamino-phenyl)-ethyl]-carbamicacid tert-butyl ester: ¹H NMR (300 MHz, MeOH-d₄) δ 7.22-7.75 (m, 2H),7.62-7.69 (m, 2H), 7.55 (s, 1H), 7.10-7.20 (m, 5H), 5.25 (m, 1H),4.27-4.36 (m, 1H), 4.11-4.21 (m, 1H), 3.33-3.44 (m, 4H), 2.84-2.90 (m,1H), 1.33 (s, 9H).

4-((S)-2-(tert-Butoxycarbonylamino)-2-(4-(thiophen-3-yl)thiazol-2-yl)ethyl)phenylsulfamic acid: ¹H NMR (300 MHz, CD₃OD): δ 7.84 (dd, J=3.0, 1.5 Hz, 1H),7.57-7.55 (m, 2H), 7.47 (dd, J=4.8, 3.0 Hz, 1H), 7.15 (s, 4H), 5.15-5.10(m, 1H), 3.39-3.34 (m, 1H), 3.01 (dd, J=14.1, 9.6 Hz, 1H), 1.42 (s, 8H),1.32 (s, 1H).

(S)-4-(2-(Benzo[d]thiazol-2-ylamino)-2-(tert-butoxycarbonyl)ethyl)phenylsulfamicacid: ¹H NMR (CD₃OD) δ 7.86-7.82 (m, 2H), 7.42 (t, 2H, J=7.1 Hz), 7.33(t, 1H, J=8.2 Hz), 7.02 (s, 4H), 5.10-5.05 (m, 1H), 2.99-2.91 (m, 2H),1.29 (s, 9H).

(S)-4-(2-tert-Butoxycarbonylamino)-2-(2-methylthiazol-4-yl)-phenylsulfamicacid ¹H NMR (300 MHz, D₂O) δ 6.99-7.002 (m, 4H), 6.82 (s, 1H), 2.26 (dd,J=13.8 and 7.2 Hz, 1H), 2.76 (dd, J=13.8 and 7.2 Hz, 1H), 2.48 (s, 3H),1.17 (s, 9H).

The first aspect of Category V of the present disclosure relates to2-(thiazol-2-yl) compounds having the formula:

wherein R¹, R², R³, and L are further defined herein in Table IX hereinbelow.

TABLE IX No. L R¹ R² R³ I272 —C(O)CH₂— phenyl —CH₃ —H I273 —C(O)CH₂—2-fluorophenyl —CH₃ —H I274 —C(O)CH₂— 3-fluorophenyl —CH₃ —H I275—C(O)CH₂— 4-fluorophenyl —CH₃ —H I276 —C(O)CH₂— 2,3-difluorophenyl —CH₃—H I277 —C(O)CH₂— 3,4-difluorophenyl —CH₃ —H I278 —C(O)CH₂—3,5-difluorophenyl —CH₃ —H I279 —C(O)CH₂— 2-chlorophenyl —CH₃ —H I280—C(O)CH₂— 3-chlorophenyl —CH₃ —H I281 —C(O)CH₂— 4-chlorophenyl —CH₃ —HI282 —C(O)CH₂— 2,3-dichlorophenyl —CH₃ —H I283 —C(O)CH₂—3,4-dichlorophenyl —CH₃ —H I284 —C(O)CH₂— 3,5-dichlorophenyl —CH₃ —HI285 —C(O)CH₂— 2-hydroxyphenyl —CH₃ —H I286 —C(O)CH₂— 3-hydroxyphenyl—CH₃ —H I287 —C(O)CH₂— 4-hydroxyphenyl —CH₃ —H I288 —C(O)CH₂—2-methoxyphenyl —CH₃ —H I289 —C(O)CH₂— 3-methoxyphenyl —CH₃ —H I290—C(O)CH₂— 4-methoxyphenyl —CH₃ —H I291 —C(O)CH₂— 2,3-dimethoxyphenyl—CH₃ —H I292 —C(O)CH₂— 3,4-dimethoxyphenyl —CH₃ —H I293 —C(O)CH₂—3,5-dimethoxyphenyl —CH₃ —H I294 —C(O)CH₂— phenyl —CH₂CH₃ —H I295—C(O)CH₂— 2-fluorophenyl —CH₂CH₃ —H I296 —C(O)CH₂— 3-fluorophenyl—CH₂CH₃ —H I297 —C(O)CH₂— 4-fluorophenyl —CH₂CH₃ —H I298 —C(O)CH₂—2,3-difluorophenyl —CH₂CH₃ —H I299 —C(O)CH₂— 3,4-difluorophenyl —CH₂CH₃—H I300 —C(O)CH₂— 3,5-difluorophenyl —CH₂CH₃ —H I301 —C(O)CH₂—2-chlorophenyl —CH₂CH₃ —H I302 —C(O)CH₂— 3-chlorophenyl —CH₂CH₃ —H I303—C(O)CH₂— 4-chlorophenyl —CH₂CH₃ —H I304 —C(O)CH₂— 2,3-dichlorophenyl—CH₂CH₃ —H I305 —C(O)CH₂— 3,4-dichlorophenyl —CH₂CH₃ —H I306 —C(O)CH₂—3,5-dichlorophenyl —CH₂CH₃ —H I307 —C(O)CH₂— 2-hydroxyphenyl —CH₂CH₃ —HI308 —C(O)CH₂— 3-hydroxyphenyl —CH₂CH₃ —H I309 —C(O)CH₂— 4-hydroxyphenyl—CH₂CH₃ —H I310 —C(O)CH₂— 2-methoxyphenyl —CH₂CH₃ —H I311 —C(O)CH₂—3-methoxyphenyl —CH₂CH₃ —H I312 —C(O)CH₂— 4-methoxyphenyl —CH₂CH₃ —HI313 —C(O)CH₂— 2,3-dimethoxyphenyl —CH₂CH₃ —H I314 —C(O)CH₂—3,4-dimethoxyphenyl —CH₂CH₃ —H I315 —C(O)CH₂— 3,5-dimethoxyphenyl—CH₂CH₃ —H I316 —C(O)CH₂CH₂— phenyl —CH₃ —H I317 —C(O)CH₂CH₂—2-fluorophenyl —CH₃ —H I318 —C(O)CH₂CH₂— 3-fluorophenyl —CH₃ —H I319—C(O)CH₂CH₂— 4-fluorophenyl —CH₃ —H I320 —C(O)CH₂CH₂— 2,3-difluorophenyl—CH₃ —H I321 —C(O)CH₂CH₂— 3,4-difluorophenyl —CH₃ —H I322 —C(O)CH₂CH₂—3,5-difluorophenyl —CH₃ —H I323 —C(O)CH₂CH₂— 2-chlorophenyl —CH₃ —H I324—C(O)CH₂CH₂— 3-chlorophenyl —CH₃ —H I325 —C(O)CH₂CH₂— 4-chlorophenyl—CH₃ —H I326 —C(O)CH₂CH₂— 2,3-dichlorophenyl —CH₃ —H I327 —C(O)CH₂CH₂—3,4-dichlorophenyl —CH₃ —H I328 —C(O)CH₂CH₂— 3,5-dichlorophenyl —CH₃ —HI329 —C(O)CH₂CH₂— 2-hydroxyphenyl —CH₃ —H I330 —C(O)CH₂CH₂—3-hydroxyphenyl —CH₃ —H I331 —C(O)CH₂CH₂— 4-hydroxyphenyl —CH₃ —H I332—C(O)CH₂CH₂— 2-methoxyphenyl —CH₃ —H I333 —C(O)CH₂CH₂— 3-methoxyphenyl—CH₃ —H I334 —C(O)CH₂CH₂— 4-methoxyphenyl —CH₃ —H I335 —C(O)CH₂CH₂—2,3-dimethoxyphenyl —CH₃ —H I336 —C(O)CH₂CH₂— 3,4-dimethoxyphenyl —CH₃—H I337 —C(O)CH₂CH₂— 3,5-dimethoxyphenyl —CH₃ —H I338 —C(O)CH₂CH₂—phenyl —CH₂CH₃ —H I339 —C(O)CH₂CH₂— 2-fluorophenyl —CH₂CH₃ —H I340—C(O)CH₂CH₂— 3-fluorophenyl —CH₂CH₃ —H I341 —C(O)CH₂CH₂— 4-fluorophenyl—CH₂CH₃ —H I342 —C(O)CH₂CH₂— 2,3-difluorophenyl —CH₂CH₃ —H I343—C(O)CH₂CH₂— 3,4-difluorophenyl —CH₂CH₃ —H I344 —C(O)CH₂CH₂—3,5-difluorophenyl —CH₂CH₃ —H I345 —C(O)CH₂CH₂— 2-chlorophenyl —CH₂CH₃—H I346 —C(O)CH₂CH₂— 3-chlorophenyl —CH₂CH₃ —H I347 —C(O)CH₂CH₂—4-chlorophenyl —CH₂CH₃ —H I348 —C(O)CH₂CH₂— 2,3-dichlorophenyl —CH₂CH₃—H I349 —C(O)CH₂CH₂— 3,4-dichlorophenyl —CH₂CH₃ —H I350 —C(O)CH₂CH₂—3,5-dichlorophenyl —CH₂CH₃ —H I351 —C(O)CH₂CH₂— 2-hydroxyphenyl —CH₂CH₃—H I352 —C(O)CH₂CH₂— 3-hydroxyphenyl —CH₂CH₃ —H I353 —C(O)CH₂CH₂—4-hydroxyphenyl —CH₂CH₃ —H I354 —C(O)CH₂CH₂— 2-methoxyphenyl —CH₂CH₃ —HI355 —C(O)CH₂CH₂— 3-methoxyphenyl —CH₂CH₃ —H I356 —C(O)CH₂CH₂—4-methoxyphenyl —CH₂CH₃ —H I357 —C(O)CH₂CH₂— 2,3-dimethoxyphenyl —CH₂CH₃—H I358 —C(O)CH₂CH₂— 3,4-dimethoxyphenyl —CH₂CH₃ —H I359 —C(O)CH₂CH₂—3,5-dimethoxyphenyl —CH₂CH₃ —H

The compounds encompassed within the first aspect of Category V of thepresent disclosure can be prepared by the procedure outlined in SchemeVII and described in Example 8 herein below.

Example 8{4-[2-(S)-(4-Ethylthiazol-2-yl)-2-(2-phenylacetylamido)ethyl]phenyl}sulfamicacid (21)

Preparation ofN-[1-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethyl]-2-phenyl-acetamide(20): To a solution of1-(S)-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethyl amine hydrobromide,3, (0.393 g, 1.1 mmol), phenylacetic acid (0.190 g, 1.4 mmol) and1-hydroxybenzotriazole (HOBt) (0.094 g, 0.70 mmol) in DMF (10 mL) at 0°,is added 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDCI) (0.268 g,1.4 mmol) followed by triethylamine (0.60 mL, 4.2 mmol). The mixture isstirred at 0° C. for 30 minutes then at room temperature overnight. Thereaction mixture is diluted with water and extracted with EtOAc. Thecombined organic phase is washed with 1 N aqueous HCl, 5% aqueousNaHCO₃, water and brine, and dried over Na₂SO₄. The solvent is removedin vacuo to afford 0.260 g (60% yield) of the desired product which isused without further purification. ESI+MS 396 (M+1).

Preparation of{4-[2-(S)-(4-ethylthiazol-2-yl)-2-(2-phenylacetylamido)ethyl]-phenyl}sulfamicacid (21):N-[1-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethyl]-2-phenyl-acetamide,20, (0.260 g) is dissolved in MeOH (4 mL). A catalytic amount of Pd/C(10% w/w) is added and the mixture is stirred under a hydrogenatmosphere 18 hours. The reaction mixture is filtered through a bed ofCELITE™ and the solvent is removed under reduced pressure. The crudeproduct is dissolved in pyridine (12 mL) and treated with SO₃-pyridine(0.177 g, 1.23). The reaction is stirred at room temperature for 5minutes after which a 7% solution of NH₄OH (10 mL) is added. The mixtureis then concentrated and the resulting residue is purified by reversephase chromatography to afford 0.136 g of the desired product as theammonium salt. ¹H NMR (CD₃OD) δ 8.60 (d, 1H, J=8.1 Hz), 7.33-7.23 (m,3H), 7.16-7.00 (m, 6H), 5.44-5.41 (m, 1H), 3.28 (1H, A of ABX, obscuredby solvent), 3.03 (1H, B of ABX, J=14.1, 9.6 Hz), 2.80 (q, 2H, J=10.5,7.8 Hz) 1.31 (t, 3H, J=4.6 Hz).

The following are non-limiting examples of the first aspect of CategoryV of the present disclosure.

(S)-4-(2-(4-Ethylthiazol-2-yl)-2-(2-(2-fluorophenyl)acetamido)ethyl)phenylsulfamicacid: ¹H NMR (CD₃OD) δ 8.65 (d, 1H, J=8.4 Hz), 7.29-7.15 (m, 1H),7.13-7.03 (m, 7H), 5.46-5.42 (m, 1H), 3.64-3.51 (m, 2H), 3.29 (1H), 3.04(1H, B of ABX, J=13.8, 9.6 Hz), 2.81 (q, 2H, J=15.6, 3.9 Hz), 1.31 (t,3H, J=7.8 Hz). ¹⁹F NMR (CD₃OD) δ 43.64.

(S)-4-(2-(4-Ethylthiazol-2-yl)-2-(2-(3-fluorophenyl)acetamido)ethyl)phenylsulfamicacid: ¹H NMR (CD₃OD) δ 8.74 (d, 1H, J=8.4 Hz), 7.32 (q, 1H, J=6.6, 14.2Hz), 7.10-6.91 (m, 8H), 5.47-5.40 (m, 1H), 3.53 (s, 2H), 3.30 (1H), 3.11(1H, B of ABX, J=9.6, 14.1 Hz), 2.80 (q, 2H, J=6.6, 15.1 Hz), 1.31 (t,3H, J=7.8 Hz). ¹⁹F NMR δ 47.42.

(S)-4-(2-(2-(2,3-Difluorophenyl)acetamido)-2-(4-ethylthiazol-2-yl)ethyl)phenyl-sulfamicacid: ¹H NMR (CD₃OD) δ 7.16-7.05 (m, 5H), 6.85-6.80 (m, 1H), 5.48-5.43(m, 1H), 3.63 (s, 2H), 3.38 (1H, A of ABX, obscured by solvent), 3.03(1H), 2.80 (q, H, J=15.1, 7.8 Hz), 1.31 (t, 3H, J=7.5 Hz).

(S)-4-(2-(2-(3,4-Difluorophenyl)acetamido)-2-(4-ethylthiazol-2-yl)ethyl)phenyl-sulfamicacid: ¹H NMR (CD₃OD) δ 8.75 (d, 1H, J=7.8 Hz), 7.23-7.04 (m, 6H),6.88-6.84 (m, 1H), 5.44-5.40 (m, 1H), 3.49 (s, 2H), 3.34 (1H), 3.02 (1H,B of ABX, J=14.1, 9.9 Hz), 2.80 (q, 2H, J=15.1, 7.8 Hz), 1.31 (t, 1H,J=7.5 Hz). ¹⁹F NMR (CD₃OD) δ 22.18, 19.45.

(S)-4-(2-(2-(2-Chlorophenyl)acetamido)-2-(4-ethylthiazol-2-yl)ethyl)phenylsulfamicacid: ¹H NMR (CD₃OD) δ 7.39-7.36 (m, 1H), 7.27-7.21 (m, 2H), 7.15-6.98(m, 5H), 5.49-5.44 (m, 1H), 3.69 (d, 2H, J=11.7 Hz), 3.32 (1H), 3.04(1H, B of ABX, J=9.3, 13.9 Hz), 2.80 (q, 2H, J=7.8, 15.3 Hz), 1.31 (t,3H, J=7.5 Hz).

(S)-4-(2-(2-(3-Chlorophenyl)acetamido)-2-(4-ethylthiazol-2-yl)ethyl)phenylsulfamicacid: ¹H NMR (CD₃OD) δ 7.33-7.23 (m, 3H), 7.13-7.03 (m, 5H), 5.43 (q,1H, J=5.1, 9.6 Hz), 3.51 (s, 2H), 3.29 (1H), 3.03 (1H, B of ABX, J=9.9,14.1 Hz), 2.80 (q, 2H, J=7.5, 15 Hz), 1.31 (t, 3H, J=7.8 Hz).

(S)-4-(2-(4-Ethylthiazol-2-yl)-2-(2-(3-hydroxyphenyl)acetamido)ethyl)phenyl-sulfamicacid: ¹H NMR (CD₃OD) δ 7.16-7.08 (m, 3H), 7.03-7.00 (m, 3H), 6.70-6.63(m, 2H), 5.42-5.40 (m, 1H), 3.44 (s, 2H), 3.28 (1H, A of ABX, obscuredby solvent), 3.04 (B of ABX, J=14.1, 9.6 Hz), 2.89 (q, 2H, J=15, 7.5Hz), 1.31 (t, 3H, J=7.5 Hz).

(S)-4-(2-(4-Ethylthiazol-2-yl)-2-(2-(2-methoxyphenypacetamido)ethyl)phenyl-sulfamicacid: ¹H NMR (CD₃OD) δ 8.00 (d, 1H, J=7.8 Hz), 7.26 (t, 1H, J=13.2 Hz),7.09-7.05 (m, 4H), 7.01 (s, 1H), 6.91-6.89 (m, 4H), 5.44-5.39 (m, 1H),3.71 (s, 3H), 3.52 (s, 2H), 3.26 (1H, A of ABX, J=14.1, 5.1 Hz), 3.06(1H B of ABX, J=13.8, 8.4 Hz), 2.80 (q, 2H, J=8.1, 15.6 Hz), 1.31 (t,3H, J=1.2 Hz).

(S)-4-{2-(4-Ethylthiazol-2-yl)-2-[2-(3-methoxyphenyl)acetamido]ethyl}-phenylsulfamicacid: ¹H NMR (CD₃OD) δ 8.58 (d, 1H, J=8.1 Hz), 7.21 (t, 1H, J=7.8 Hz),7.12-7.02 (m, 4H), 6.81 (s, 2H), 6.72 (d, 1H, J=7.5 Hz), 5.45-5.40 (m,1H), 3.79 (s, 3H), 3.50 (s, 2H), 3.29 (1H, A of ABX, obscured bysolvent), 3.08 (1H, B of ABX, J=11.8, 5.1 Hz), 2.80 (q, 2H, J=15, 7.5Hz), 1.31 (t, 3H, J=6.6 Hz).

(S)-4-(2-(4-Ethylthiazol-2-yl)-2-(3-phenylpropanamido)ethyl)phenylsulfamicacid: ¹H NMR (CD₃OD) δ 8.56 (d, 1H, J=8.4 Hz), 7.25-6.98 (m, 9H),5.43-5.38 (m, 1H), 3.26 (1H, A of ABX, J=14.1, 9.6 Hz), 2.97 (1H, B ofABX, J=10.9, 3 Hz), 2.58-2.76 (m, 3H), 2.98 (q, 2H, J=13.8, 7.2 Hz),1.29 (t, 3H, J=8.7 Hz).

(S)-4-(2-(2-(3,4-Dimethoxyphenyl)acetamido)-2-(4-ethylthiazol-2-yl)ethyl)-phenylsulfamicacid: ¹H NMR (CD₃OD) δ 7.12-7.03 (m, 3H), 6.91 (d, 1H, J=8.4 Hz), 6.82(s, 1H), 6.66 (d, 1H, J=2.1 Hz), 6.63 (d, 1H, J=2.1 Hz), 5.43 (m, 1H),3.84 (s, 3H), 3.80 (s, 3H), 3.45 (s, 2H), 3.30 (1H), 3.03 (1H, B of ABX,J=14.1, 9.6 Hz), 2.79 (q, 2H, J=15.1, 7.2 Hz), 1.30 (t, 3H, J=7.2 Hz).

(S)-4-(2-(2-(2,3-Dimethoxyphenyl)acetamido)-2-(4-ethylthiazol-2-yl)ethyl)-phenylsulfamicacid: ¹H NMR (CD₃OD) δ 8.31 (d, 1H, J=7.8 Hz), 7.11-6.93 (m, 6H), 6.68(d, 1H, J=7.5 Hz), 5.49-5.40 (m, 1H), 3.87 (s, 3H), 3.70 (s, 3H), 3.55(s, 2H), 3.26 (1H, A of ABX, obscured by solvent), 3.06 (1H, B of ABX,J=13.9, 9 Hz), 2.80 (q, 2H, J=14.8, 7.5 Hz), 1.31 (t, 3H, J=7.5 Hz).

(S)-4-(2-(3-(3-Chlorophenyl)propanamido)-2-(4-ethylthiazol-2-yl)ethyl)phenyl-sulfamicacid: ¹H NMR (CD₃OD) δ 7.27-7.18 (m, 3H), 7.13-7.08 (m, 5H), 7.01 (s,1H), 5.39 (q, 1H, J=5.1, 9.4 Hz), 3.28 (1H, A of ABX, J=5.1, 14.1 Hz),2.97 (1H, B of ABX, J=9.3, 13.9 Hz), 2.88-2.76 (m, 4H), 2.50 (t, 2H,J=8.1 Hz), 1.31 (t, 3H, J=7.8 Hz).

(S)-4-(2-(4-Ethylthiazol-2-yl)-2-(3-(2-methoxyphenyl)propanamido)ethyl)phenyl-sulfamicacid: ¹H NMR (CD₃OD) δ 7.18-7.08 (m, 6H), 6.92 (d, 1H, J=8.1 Hz), 6.82(t, 1H, J=7.5 Hz), 5.40-5.35 (m, 1H), 3.25 (1H, A of ABX, J=15, 5.4 Hz),3.00 (1H, B of ABX, J=10.5, 7.5 Hz), 2.88-2.76 (m, 4H), 2.47 (q, 2H,J=9.1, 6 Hz), 1.31 (t, 3H, J=7.8 Hz).

(S)-4-(2-(4-Ethylthiazol-2-yl)-2-(3-(3-methoxyphenyl)propanamido)ethyl)phenyl-sulfamicacid: ¹H NMR (CD₃OD) δ 7.19-7.00 (m, 5H), 6.75 (s, 1H), 6.73 (s, 1H),5.42-5.37 (m, 1H), 3.76 (s, 3H), 3.25 (1H, A of ABX, J=13.9, 5.4 Hz),2.98 (1H, B of ABX, J=14.1, 9.6 Hz), 2.86-2.75 (m, 4H), 2.48 (q, 2H,J=11.7, 1.2 Hz), 1.31 (t, 3H, J=7.5 Hz).

(S)-4-(2-(4-Ethylthiazol-2-yl)-2-(3-(4-methoxyphenyl)propanamido)ethyl)phenyl-sulfamicacid: ¹H NMR (CD₃OD) δ 7.13-6.99 (m, 7H), 6.82-6.78 (m, 2H), 5.42-5.37(m, 1H), 3.33 (s, 3H), 3.23 (1H), 2.97 (1H, B of ABX, J=13.3, 11.4 Hz),2.83-2.75 (m, 4H), 2.49 (q, 2H, J=6.4, 3.3 Hz), 1.31 (t, 3H, J=7.5 Hz).

(S)-4-{2-[2-(4-Ethyl-2,3-dioxopiperazin-1-yl)acetamido]-2-(4-ethylthiazol-2-yl)ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD) δ 7.14 (s, 4H), 7.08 (s, 1H), 5.56-5.51 (m, 1H),4.34 (d, 2H, J=16.2 Hz), 3.88 (d, 2H, J=17.6 Hz), 3.59-3.40 (m, 3H),3.26-3.14 (m, 3H), 2.98 (1H, B of ABX, J=10.8, 13.9 Hz), 2.82 (q, 2H,J=6.9, 15 Hz), 1.32 (t, 3H, J=7.5 Hz), 1.21 (t, 3H, J=7.2 Hz).

(S)-4-{2-(4-Ethylthiazol-2-yl)-2-[2-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetamido]ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.13 (s, 1H), 7.06-7.02 (m, 4H), 6.95 (s, 1H),5.42-5.31 (m, 1H), 4.43-4.18 (dd, 2H, J=16.5 Hz), 3.24-2.93 (m, 2H),2.74-2.69 (q, 2H, J=7.3 Hz), 1.79 (s, 3H), 1.22 (t, 3H, J=7.5 Hz).

(S)-4-[2-(benzo[d][1,3]dioxole-5-carboxamido)-2-(4-ethylthiazol-2-yl)ethyl]-phenylsulfamicacid: ¹H NMR (CD₃OD) δ 7.25 (d, 1H, J=6.5 Hz), 7.13 (s, 1H), 7.06 (d,2H, J=8.5 Hz), 7.00 (d, 2H, J=8.5 Hz), 6.91 (s, 1H), 6.76 (d, 1H, J=8.1Hz), 5.90 (s, 2H), 5.48 (q, 1H, J=5.0 Hz), 3.32-3.24 (m, 2H), 3.07-2.99(m, 2H), 2.72 (q, 2H, J=7.5 Hz), 1.21 (t, 3H, J=7.5 Hz).

(S)-4-{2-[2-(2,5-Dimethylthiazol-4-yl)acetamido]-2-(4-ethylthiazol-2-yl)ethyl}-phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.10-7.01 (m, 5H), 5.41 (t, 1H, J=6.9 Hz), 3.58(s, 2H), 3.33-3.01 (m, 2H), 2.82-2.75 (q, 2H, J=7.5 Hz), 2.59 (s, 3H),2.23 (s, 3H), 1.30 (t, 3H, J=7.5 Hz).

(S)-4-{2-[2-(2,4-Dimethylthiazol-5-yl)acetamido]-2-(4-methylthiazol-2-yl)ethyl}-phenylsulfamicacid: ¹H NMR (CD₃OD): δ 8.71-8.68 (d, 1H, J=8.4 Hz), 7.10-7.03 (m, 4H),7.01 (s, 1H), 5.41 (m, 1H), 3.59 (s, 1H), 3.34-2.96 (m, 2H), 2.59 (s,3H), 2.40 (s, 3H), 2.23 (s, 3H).

(S)-4-{2-(4-Ethylthiazol-2-yl)-2-[3-(thiazol-2-yl)propanamido]ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.67-7.65 (m, 1H), 7.49-7.47 (m, 1H), 7.14-7.08(m, 4H), 7.04 (s, 1H), 5.46-5.41 (q, 1H, J=5.1 Hz), 3.58 (s, 2H),3.30-3.25 (m, 3H), 3.02-2.67 (m, 5H), 1.31 (t, 3H, J=7.5 Hz).

(S)-4-{2-(4-Ethylthiazol-2-yl)-2-[2-(4-ethylthiazol-2-yl)acetamido]ethyl}phenyl-sulfamicacid: ¹H NMR (CD₃OD): δ 7.04-6.91 (m, 6H), 5.32 (t, 1H, J=5.4 Hz),3.25-2.90 (m, 2H), 2.71-2.61 (m, 4H) 1.93 (s, 2H) 1.22-1.14 (m, 6H).

The second aspect of Category V of the present disclosure relates to2-(thiazol-4-yl) compounds having the formula:

wherein R¹, R⁴, and L are further defined herein in Table X hereinbelow.

TABLE X No. L R¹ R⁴ J360 —C(O)CH₂— Phenyl methyl J361 —C(O)CH₂— Phenylethyl J362 —C(O)CH₂— Phenyl phenyl J363 —C(O)CH₂— phenyl thiophen-2-ylJ364 —C(O)CH₂— phenyl thiazol-2-yl J365 —C(O)CH₂— phenyl oxazol-2-ylJ366 —C(O)CH₂— phenyl isoxazol-3-yl J367 —C(O)CH₂— 3-chlorophenyl methylJ368 —C(O)CH₂— 3-chlorophenyl ethyl J369 —C(O)CH₂— 3-chlorophenyl phenylJ370 —C(O)CH₂— 3-chlorophenyl thiophen-2-yl J371 —C(O)CH₂—3-chlorophenyl thiazol-2-yl J372 —C(O)CH₂— 3-chlorophenyl oxazol-2-ylJ373 —C(O)CH₂— 3-chlorophenyl isoxazol-3-yl J374 —C(O)CH₂—3-methoxyphenyl methyl J375 —C(O)CH₂— 3-methoxyphenyl ethyl J376—C(O)CH₂— 3-methoxyphenyl phenyl J377 —C(O)CH₂— 3-methoxyphenylthiophen-2-yl J378 —C(O)CH₂— 3-methoxyphenyl thiazol-2-yl J379 —C(O)CH₂—3-methoxyphenyl oxazol-2-yl J380 —C(O)CH₂— 3-methoxyphenyl isoxazol-3-ylJ381 —C(O)CH₂— 3-fluorophenyl methyl J382 —C(O)CH₂— 3-fluorophenyl ethylJ383 —C(O)CH₂— 3-fluorophenyl phenyl J384 —C(O)CH₂— 3-fluorophenylthiophen-2-yl J385 —C(O)CH₂— 3-fluorophenyl thiazol-2-yl J386 —C(O)CH₂—3-fluorophenyl oxazol-2-yl J387 —C(O)CH₂— 3-fluorophenyl isoxazol-3-ylJ388 —C(O)CH₂— 2,5-dimethylthiazol-4-yl methyl J389 —C(O)CH₂—2,5-dimethylthiazol-4-yl ethyl J390 —C(O)CH₂— 2,5-dimethylthiazol-4-ylphenyl J391 —C(O)CH₂— 2,5-dimethylthiazol-4-yl thiophen-2-yl J392—C(O)CH₂— 2,5-dimethylthiazol-4-yl thiazol-2-yl J393 —C(O)CH₂—2,5-dimethylthiazol-4-yl oxazol-2-yl J394 —C(O)CH₂—2,5-dimethylthiazol-4-yl isoxazol-3-yl J395 —C(O)CH₂—2,4-dimethylthiazol-5-yl methyl J396 —C(O)CH₂— 2,4-dimethylthiazol-5-ylethyl J397 —C(O)CH₂— 2,4-dimethylthiazol-5-yl phenyl J398 —C(O)CH₂—2,4-dimethylthiazol-5-yl thiophen-2-yl J399 —C(O)CH₂—2,4-dimethylthiazol-5-yl thiazol-2-yl J400 —C(O)CH₂—2,4-dimethylthiazol-5-yl oxazol-2-yl J401 —C(O)CH₂—2,4-dimethylthiazol-5-yl isoxazol-3-yl J402 —C(O)CH₂—4-ethylthiazol-2-yl methyl J403 —C(O)CH₂— 4-ethylthiazol-2-yl ethyl J404—C(O)CH₂— 4-ethylthiazol-2-yl phenyl J405 —C(O)CH₂— 4-ethylthiazol-2-ylthiophen-2-yl J406 —C(O)CH₂— 4-ethylthiazol-2-yl thiazol-2-yl J407—C(O)CH₂— 4-ethylthiazol-2-yl oxazol-2-yl J408 —C(O)CH₂—4-ethylthiazol-2-yl isoxazol-3-yl J409 —C(O)CH₂—3-methyl-1,2,4-oxadiazol- methyl 5-yl J410 —C(O)CH₂—3-methyl-1,2,4-oxadiazol- ethyl 5-yl J411 —C(O)CH₂—3-methyl-1,2,4-oxadiazol- phenyl 5-yl J412 —C(O)CH₂—3-methyl-1,2,4-oxadiazol- thiophen-2-yl 5-yl J413 —C(O)CH₂—3-methyl-1,2,4-oxadiazol- thiazol-2-yl 5-yl J414 —C(O)CH₂—3-methyl-1,2,4-oxadiazol- oxazol-2-yl 5-yl J415 —C(O)CH₂—3-methyl-1,2,4-oxadiazol- isoxazol-3-yl 5-yl J416 —C(O)CH₂CH₂— phenylmethyl J417 —C(O)CH₂CH₂— phenyl ethyl J418 —C(O)CH₂CH₂— phenyl phenylJ419 —C(O)CH₂CH₂— phenyl thiophen-2-yl J420 —C(O)CH₂CH₂— phenylthiazol-2-yl J421 —C(O)CH₂CH₂— phenyl oxazol-2-yl J422 —C(O)CH₂CH₂—phenyl isoxazol-3-yl J423 —C(O)CH₂CH₂— 3-chlorophenyl methyl J424—C(O)CH₂CH₂— 3-chlorophenyl ethyl J425 —C(O)CH₂CH₂— 3-chlorophenylphenyl J426 —C(O)CH₂CH₂— 3-chlorophenyl thiophen-2-yl J427 —C(O)CH₂CH₂—3-chlorophenyl thiazol-2-yl J428 —C(O)CH₂CH₂— 3-chlorophenyl oxazol-2-ylJ429 —C(O)CH₂CH₂— 3-chlorophenyl isoxazol-3-yl J430 —C(O)CH₂CH₂—3-methoxyphenyl methyl J431 —C(O)CH₂CH₂— 3-methoxyphenyl ethyl J432—C(O)CH₂CH₂— 3-methoxyphenyl phenyl J433 —C(O)CH₂CH₂— 3-methoxyphenylthiophen-2-yl J434 —C(O)CH₂CH₂— 3-methoxyphenyl thiazol-2-yl J435—C(O)CH₂CH₂— 3-methoxyphenyl oxazol-2-yl J436 —C(O)CH₂CH₂—3-methoxyphenyl isoxazol-3-yl J437 —C(O)CH₂CH₂— 3-fluorophenyl methylJ438 —C(O)CH₂CH₂— 3-fluorophenyl ethyl J439 —C(O)CH₂CH₂— 3-fluorophenylphenyl J440 —C(O)CH₂CH₂— 3-fluorophenyl thiophen-2-yl J441 —C(O)CH₂CH₂—3-fluorophenyl thiazol-2-yl J442 —C(O)CH₂CH₂— 3-fluorophenyl oxazol-2-ylJ443 —C(O)CH₂CH₂— 3-fluorophenyl isoxazol-3-yl J444 —C(O)CH₂CH₂—2,5-dimethylthiazol-4-yl methyl J445 —C(O)CH₂CH₂—2,5-dimethylthiazol-4-yl ethyl J446 —C(O)CH₂CH₂—2,5-dimethylthiazol-4-yl phenyl J447 —C(O)CH₂CH₂—2,5-dimethylthiazol-4-yl thiophen-2-yl J448 —C(O)CH₂CH₂—2,5-dimethylthiazol-4-yl thiazol-2-yl J449 —C(O)CH₂CH₂—2,5-dimethylthiazol-4-yl oxazol-2-yl J450 —C(O)CH₂CH₂—2,5-dimethylthiazol-4-yl isoxazol-3-yl J451 —C(O)CH₂CH₂—2,4-dimethylthiazol-5-yl methyl J452 —C(O)CH₂CH₂—2,4-dimethylthiazol-5-yl ethyl J453 —C(O)CH₂CH₂—2,4-dimethylthiazol-5-yl phenyl J454 —C(O)CH₂CH₂—2,4-dimethylthiazol-5-yl thiophen-2-yl J455 —C(O)CH₂CH₂—2,4-dimethylthiazol-5-yl thiazol-2-yl J456 —C(O)CH₂CH₂—2,4-dimethylthiazol-5-yl oxazol-2-yl J457 —C(O)CH₂CH₂—2,4-dimethylthiazol-5-yl isoxazol-3-yl J458 —C(O)CH₂CH₂—4-ethylthiazol-2-yl methyl J459 —C(O)CH₂CH₂— 4-ethylthiazol-2-yl ethylJ460 —C(O)CH₂CH₂— 4-ethylthiazol-2-yl phenyl J461 —C(O)CH₂CH₂—4-ethylthiazol-2-yl thiophen-2-yl J462 —C(O)CH₂CH₂— 4-ethylthiazol-2-ylthiazol-2-yl J463 —C(O)CH₂CH₂— 4-ethylthiazol-2-yl oxazol-2-yl J464—C(O)CH₂CH₂— 4-ethylthiazol-2-yl isoxazol-3-yl J465 —C(O)CH₂CH₂—3-methyl-1,2,4-oxadiazol- methyl 5-yl J466 —C(O)CH₂CH₂—3-methyl-1,2,4-oxadiazol- ethyl 5-yl J467 —C(O)CH₂CH₂—3-methyl-1,2,4-oxadiazol- phenyl 5-yl J468 —C(O)CH₂CH₂—3-methyl-1,2,4-oxadiazol- thiophen-2-yl 5-yl J469 —C(O)CH₂CH₂—3-methyl-1,2,4-oxadiazol- thiazol-2-yl 5-yl J470 —C(O)CH₂CH₂—3-methyl-1,2,4-oxadiazol- oxazol-2-yl 5-yl J471 —C(O)CH₂CH₂—3-methyl-1,2,4-oxadiazol- isoxazol-3-yl 5-yl

The compounds encompassed within the second aspect of Category I of thepresent disclosure can be prepared by the procedure outlined in SchemeII and described in Example 9 herein below.

Example 94-((S)-2-(2-(3-chlorophenyl)acetamido)-2-(2-(thiophen-2-yl)thiazol-4-yl)ethyl)phenylsulfamicacid (23)

Preparation of(S)-2-(4-nitrophenyl)-1-[(thiophen-2-yl)thiazol-4-yl]ethanaminehydrobromide salt (22): A mixture of (S)-tert-butyl4-bromo-1-(4-nitrophenyl)-3-oxobutan-2-ylcarbamate, 7, (7.74 g, 20mmol), and thiophen-2-carbothioic acid amide (3.14 g, 22 mmol) in CH₃CN(200 mL) is refluxed for 5 hours. The reaction mixture is cooled to roomtemperature and diethyl ether (50 mL) is added to the solution. Theprecipitate which forms is collected by filtration. The solid is driedunder vacuum to afford 7.14 g (87% yield) of the desired product. ESI+MS332 (M+1).

Preparation of2-(3-chlorophenyl)-N-{(S)-2-(4-nitrophenyl)-1-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}acetamide(23): To a solution of2-(4-nitrophenyl)-1-(2-thiophene2-ylthiazol-4-yl)ethylamine, 22, (0.41g, 1 mmol) 3-chlorophenylacetic acid (0.170 g, 1 mmol) and1-hydroxybenzotriazole (HOBt) (0.070 g, 0.50 mmol) in DMF (5 mL) at 0°,is added 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDCI) (0.190 g,1 mmol) followed by triethylamine (0.42 mL, 3 mmol). The mixture isstirred at 0° C. for 30 minutes then at room temperature overnight. Thereaction mixture is diluted with water and extracted with EtOAc. Thecombined organic phase is washed with 1 N aqueous HCl, 5% aqueousNaHCO₃, water and brine, and dried over Na₂SO₄. The solvent is removedin vacuo to afford 0.290 g (60% yield) of the desired product which isused without further purification. ESI− MS 482 (M−1).

Preparation of{4-[2-(3-chlorophenyl)acetylamino]-2-(2-thiophen-2-ylthiazol-4-yl)ethyl]phenyl}sulfamicacid (24):2-(3-chlorophenyl)-N-{(S)-2-(4-nitrophenyl)-1-[2-(thiophene2-yl)thiazol-4-yl]ethyl}acetamide,23, (0.290 g) is dissolved in MeOH (4 mL). A catalytic amount of Pd/C(10% w/w) is added and the mixture is stirred under a hydrogenatmosphere 18 hours. The reaction mixture is filtered through a bed ofCELITE™ and the solvent is removed under reduced pressure. The crudeproduct is dissolved in pyridine (12 mL) and treated with SO₃-pyridine(0.157 g). The reaction is stirred at room temperature for 5 minutesafter which a 7% solution of NH₄OH is added. The mixture is thenconcentrated and the resulting residue is purified by reverse phasechromatography to afford 0.078 g of the desired product as the ammoniumsalt. ¹H NMR (CD₃OD) δ 7.61 (d, 1H, J=3.6 Hz), 7.58 (d, 1H, J=5.1 Hz),7.41-7.35 (m, 1H), 7.28-7.22 (m, 2H), 7.18-6.98 (m, 6H), 5.33 (t, 1H,J=6.6 Hz), 3.70 (d, 2H, J=3.9 Hz), 3.23 (1H, A of ABX, J=6.6, 13.8 Hz),3.07 (1H, B of ABX, J=8.1, 13.5 Hz).

The following are non-limiting examples of compounds encompassed withinthe second aspect of Category V of the present disclosure.

4-((S)-2-(2-(3-Methoxyphenyl)acetamido)-2-(2-(thiophene2-yl)thiazol-4-yl)ethyl)-phenylsulfamicacid: ¹H NMR (CD₃OD) δ 8.35 (d, 1H, J=8.7 Hz), 7.61-7.57 (m, 2H),7.25-7.20 (m, 2H), 7.25-7.20 (m, 2H), 7.09 (s, 1H), 7.05 (d, 2H, J=4.2Hz), 6.99 (d, 1H, J=8.7 Hz), 6.81 (d, 1H, J=7.8 Hz), 6.77 (s, 1H),5.30-5.28 (m, 1H), 3.76 (s, 3H), 3.51 (s, 2H), 3.20 (1H, A of ABX,J=6.3, 13.6 Hz), 3.06 (1H, B of ABX, J=8.1, 13.8 Hz).

4-{(S)-2-(3-Phenylpropanamido)-2-[2-(thiophene2-yl)thiazol-4-yl]ethyl}-phenylsulfamicacid: ¹H NMR (CD₃OD) δ 8.30 (d, 1H, J=9 Hz), 7.61-7.56 (m, 2H),7.26-7.14 (m, 7H), 7.12 (d, 1H, J=1.5 Hz), 7.09 (d, 1H, J=2.1 Hz), 6.89(s, 1H), 5.28-5.26 (m, 1H), 3.18 (1H, A of ABX, J=6.2, 13.8 Hz), 2.96(1H, B of ABX, J=8.4, 13.6 Hz).

4-{(S)-2-(3-(3-Chlorophenyl)propanamido)-2-[2-(thiophene2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD) δ 7.61-7.56 (m, 3H), 7.22-7.14 (m, 6H), 7.08 (d,1H), 7.00 (d, 1H, J=77.5 Hz), 6.870 (s, 1H), 5.25 (t, 1H, J=7.8 Hz),3.18 (1H, A of ABX, J=6.6, 13.8 Hz), 2.97 (1H, B of ABX, J=7.8, 13.8Hz), 2.87 (t, 2H, J=7.5 Hz), 2.51 (t, 2H, J=7.2 Hz).

4-{(S)-2-[2-(3-Fluorophenyl)acetamido]-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid: ¹HNMR (CD₃OD) δ 7.61-7.57 (m, 2H), 7.32-7.28 (m, 1H), 7.19-7.16(m, 2H), 7.08 (t, 1H, J=4.5 Hz), 7.02-6.95 (m, 6H), 5.29 (t, 1H, J=8.1Hz), 3.53 (s, 2H), 3.22 (1H, A of ABX, J=6.6, 13.9 Hz), 3.06 (1H, B ofABX, J=8.4, 13.6 Hz).

(S)-4-{2-[2-(3-Methyl-1,2,4-oxadiazol-5-yl)acetamido]-2-(2-phenylthiazol-4-yl)ethyl}phenylsulfamicacid: ¹HNMR (CD₃OD): δ 7.98-7.95 (m, 2H), 7.48-7.46 (m, 3H), 7.23 (s,1H), 7.09-7.05 (m, 4H), 5.33 (t, 1H, J=7.2 Hz), 3.33-3.06 (m, 2H), 2.35(s, 3H).

4-{(S)-2-[2-(4-ethyl-2,3-dioxopiperazin-1-yl)acetamido]-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid: ¹HNMR (CD₃OD) δ 7.62 (d, 1H, J=3 Hz), 7.58 (d, 1H, J=15.6 Hz),7.27 (s, 1H), 7.16 (t, 1H, J=1.5 Hz), 5.42-5.32 (m, 1H), 4.31 (d, 1H,J=15.6 Hz), 3.91 (d, 1H, J=15.9 Hz), 3.60-3.50 (m, 4H), 3.30-3.23 (m,2H), 2.98 (1H, B of ABX, J=9.9, 13.8 Hz), 1.21 (t, 3H, J=6.9 Hz).

The third aspect of Category V of the present disclosure relates tocompounds having the formula:

wherein the linking unit L comprises a phenyl unit, said linking grouphaving the formula:

—C(O)[(CR^(5a)H)][(CR^(6a)H)]—

R¹ is hydrogen, R^(6a) is phenyl, R^(5a) is phenyl or substituted phenyland non-limiting examples of the units R², R³, and R^(5a) are furtherexemplified herein below in Table XI.

TABLE XI No. R² R³ R^(5a) K472 methyl hydrogen phenyl K473 methylhydrogen 2-fluorophenyl K474 methyl hydrogen 3-fluorophenyl K475 methylhydrogen 4-fluorophenyl K476 methyl hydrogen 3,4-difluorophenyl K477methyl hydrogen 2-chlorophenyl K478 methyl hydrogen 3-chlorophenyl K479methyl hydrogen 4-chlorophenyl K480 methyl hydrogen 3,4-dichlorophenylK481 methyl hydrogen 2-methoxyphenyl K482 methyl hydrogen3-methoxyphenyl K483 methyl hydrogen 4-methoxyphenyl K484 ethyl hydrogenphenyl K485 ethyl hydrogen 2-fluorophenyl K486 ethyl hydrogen3-fluorophenyl K487 ethyl hydrogen 4-fluorophenyl K488 ethyl hydrogen3,4-difluorophenyl K489 ethyl hydrogen 2-chlorophenyl K490 ethylhydrogen 3-chlorophenyl K491 ethyl hydrogen 4-chlorophenyl K492 ethylhydrogen 3,4-dichlorophenyl K493 ethyl hydrogen 2-methoxyphenyl K494ethyl hydrogen 3-methoxyphenyl K495 ethyl hydrogen 4-methoxyphenyl

The compounds encompassed within the third aspect of Category V of thepresent disclosure can be prepared by the procedure outlined in SchemeIX and described in Example 10 herein below.

Example 10(S)-4-(2-(2,3-Diphenylpropanamido)-2-(4-ethylthiazol-2-yl)ethyl)-phenylsulfamicacid (26)

Preparation of(S)—N-[1-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethyl]-2,3-diphenyl-propanamide(25): To a solution of1-(S)-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethyl amine hydrobromide,3, (0.95 g, 2.65 mmol), diphenylpropionic acid (0.60 g, 2.65 mmol) and1-hydroxybenzotriazole (HOBt) (0.180 g, 1.33 mmol) in DMF (10 mL) at 0°,is added 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDCI) (0.502 g,2.62 mmol) followed by triethylamine (1.1 mL, 7.95 mmol). The mixture isstirred at 0° C. for 30 minutes then at room temperature overnight. Thereaction mixture is diluted with water and extracted with EtOAc. Thecombined organic phase is washed with 1 N aqueous HCl, 5% aqueousNaHCO₃, water and brine, and dried over Na₂SO₄. The solvent is removedin vacuo to afford 0.903 g (70% yield) of the desired product which isused without further purification.

Preparation of(S)-4-(2-(2,3-diphenylpropanamido)-2-(4-ethylthiazol-2-yl)ethyl)phenylsulfamicacid (26)(S)—N-[1-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethyl]-2,3-diphenyl-propanamide,25, (0.903 g) is dissolved in MeOH (10 mL). A catalytic amount of Pd/C(10% w/w) is added and the mixture is stirred under a hydrogenatmosphere 18 hours. The reaction mixture is filtered through a bed ofCELITE™ and the solvent is removed under reduced pressure. The crudeproduct is dissolved in pyridine (30 mL) and treated with SO₃-pyridine(0.621 g). The reaction is stirred at room temperature for 5 minutesafter which a 7% solution of NH₄OH is added. The mixture is thenconcentrated and the resulting residue is purified by reverse phasechromatography to afford 0.415 g of the desired product as the ammoniumsalt. ¹H NMR (CD₃OD) δ 8.59-8.52 (m, 1H), 7.37-7.04 (m, 9H), 6.97-6.93(m, 1H), 6.89-6.85 (m, 2H), 5.36-5.32 (m, 1H), 3.91-3.83 (m, 1H), 3.29(1H, A of ABX, obscured by solvent), 3.15 (1H, B of ABX, J=5.4, 33.8Hz), 2.99-2.88 (m, 2H), 2.81-2.69 (m, 2H), 1.32-1.25 (m, 3H).

The precursors of many of the Z units which comprise the third aspect ofCategory V are not readily available. The following procedureillustrates an example of the procedure which can be used to providedifferent R^(5a) units according to the present disclosure. Using theprocedure outlined in Scheme X and described in Example 11 the artisancan make modifications without undue experimentation to achieve theR^(5a) units encompassed by the present disclosure.

Example 11 2-(2-Methoxyphenyl)-3-phenylpropanoic acid (28)

Preparation of methyl 2-(2-methoxyphenyl)-3-phenylpropanoate (27): A 500mL round-bottom flask is charged with methyl 2-(2-methoxyphenyl)acetate(8.496 g, 47 mmol, 1 eq) and THF (200 mL). The homogeneous mixture iscooled to 0° C. in an ice bath. Lithium diisopropyl amide (23.5 mL of a2.0M solution in heptane/THF) is added, maintaining a temperature lessthan 3° C. The reaction is stirred 45 minutes at this reducedtemperature. Benzyl bromide (5.6 mL, 47 mmol, 1 eq) is added dropwise.The reaction is allowed to gradually warm to room temperature and isstirred for 18 hours. The reaction is quenched with 1N HCl and extracted3 times with equal portions of EtOAc. The combined extracts are washedwith H₂O and brine, dried over Na₂SO₄, filtered, and concentrated. Theresidue is purified over silica to afford 4.433 g (35%) of the desiredcompound. ESI+MS 293 (M+Na).

Preparation of 2-(2-methoxyphenyl)-3-phenylpropanoic acid (28): Methyl2-(2-methoxyphenyl)-3-phenylpropanoate (4.433 g, 16 mmol, 1 eq) isdissolved in 100 mL of a 1:1 (v:v) mixture of THF and methanol. Sodiumhydroxide (3.28 g, 82 mmol, 5 eq) is added and the reaction mixture isstirred 18 hours at room temperature. The reaction is then poured intoH₂O and the pH is adjusted to 2 via addition of 1N HCl. A whiteprecipitate forms which is removed by filtration. The resulting solutionis extracted with 3 portion of diethyl ether. The extracts are pooled,washed with H₂O and brine, dried over Na₂SO₄, filtered, and concentratedin vacuo. The resulting residue is purified over silica to afford 2.107g (51%) of the desired compound. ESI-MS 255 (M−1), 211 (M—CO₂H).

Intermediate 28 can be carried forward according to the procedureoutlined in Scheme IX and described in Example 10 to produce thefollowing compound according to the third aspect of Category V.

(S)-4-{2-(4-Ethylthiazol-2-yl)-2-[2-(2-methoxyphenyl)-3-phenylpropanamido]-ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD) δ 7.32-7.12 (m, 7H), 7.05-7.02 (m, 1H), 6.99-6.83(m, 4H), 6.80-6.75 (m, 2H), 5.35-5.31 (m, 1H), 4.31-4.26 (m, 1H), 3.75(s, 3H), 3.20-2.90 (m, 4H), 2.79-2.74 (m, 2H), 1.32-1.25 (m, 3H).

The following are further non-limiting examples of compounds accordingto the third aspect of Category I of the present disclosure.

(S)-4-{2-(4-Ethylthiazol-2-yl)-2-[2-(3-fluorophenyl)-3-phenylpropanamido]-ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD) δ 7.33-6.87 (m, 14H), 5.39-5.25 (m, 1H), 3.95-3.83(m, 1H), 3.31-3.10 (m, 1H), 3.05-2.88 (m, 2H), 2.80-2.70 (m, 2H),1.32-1.23 (m, 3H). ¹⁹F NMR δ 47.59.

(S)-4-{2-(4-Ethylthiazol-2-yl)-2-[2-(3-methoxyphenyl)-3-phenylpropanamido]-ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD) δ 7.85 (d, 1H, J=8.4 Hz), 7.25-7.20 (m, 1H),7.11-7.02 (m, 4H), 7.01 (s, 1H), 6.90-6.79 (m, 2H), 5.45-5.40 (m, 1H),4.09 (s, 2H), 3.79 (s, 3H), 3.12-3.08 (m, 2H), 1.10 (s, 9H).

The fourth aspect of Category V of the present disclosure relates tocompounds having the formula:

wherein the linking unit L comprises a phenyl unit, said linking grouphaving the formula:

—C(O)[(CR^(5a)H)][(CR^(6a)H]—

R¹ is hydrogen, R^(6a) is phenyl, R^(5a) is substituted or unsubstitutedheteroaryl and the units R², R³, and R^(5a) are further exemplifiedherein below in Table XII.

TABLE XII No. R² R³ R^(5a) L496 methyl hydrogen3-methyl-1,2,4-oxadiazol-5-yl L497 methyl hydrogen thiophen-2-yl L498methyl hydrogen thiazol-2-yl L499 methyl hydrogen oxazol-2-yl L500methyl hydrogen isoxazol-3-yl L501 ethyl hydrogen3-methyl-1,2,4-oxadiazol-5-yl L502 ethyl hydrogen thiophen-2-yl L503ethyl hydrogen thiazol-2-yl L504 ethyl hydrogen oxazol-2-yl L505 ethylhydrogen isoxazol-3-yl L506 ethyl methyl 3-methyl-1,2,4-oxadiazol-5-ylL507 ethyl methyl thiophen-2-yl L508 ethyl methyl thiazol-2-yl L509ethyl methyl oxazol-2-yl L510 ethyl methyl isoxazol-3-yl L511thiophen-2-yl hydrogen 3-methyl-1,2,4-oxadiazol-5-yl L512 thiophen-2-ylhydrogen thiophen-2-yl L513 thiophen-2-yl hydrogen thiazol-2-yl L514thiophen-2-yl hydrogen oxazol-2-yl L515 thiophen-2-yl hydrogenisoxazol-3-yl L516 isoxazol-3-yl hydrogen 3-methyl-1,2,4-oxadiazol-5-ylL517 isoxazol-3-yl hydrogen thiophen-2-yl L518 isoxazol-3-yl hydrogenthiazol-2-yl L519 isoxazol-3-yl hydrogen oxazol-2-yl L520 isoxazol-3-ylhydrogen isoxazol-3-yl

The compounds encompassed within the fourth aspect of Category V of thepresent disclosure can be prepared by the procedure outlined in Scheme Vand described in Example 5 herein below.

Example 12

4-{(S)-2-(4-Ethylthiazol-2-yl)-2-[2-(3-methyl-1,2,4-oxadiazol-5-yl)-3-phenylpropanamido]ethyl}phenylsulfamicacid (31)

Preparation ofethyl-2-benzyl-3-[(S)-1-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)-ethylamino]-3-oxopropanoate(29): To a solution of1-(S)-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethyl amine hydrobromide,3, (0.406 g, 1.13 mmol), 2-benzyl-3-ethoxy-3-oxopropanoic acid (0.277 g)and 1-hydroxybenzotriazole (HOBt) (0.191 g, 1.41 mmol) in DMF (10 mL) at0°, is added 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDCI) (0.240g, 1.25 mmol) followed by diisopropylethylamine (DIPEA) (0.306 g). Themixture is stirred at 0° C. for 30 minutes then at room temperatureovernight. The reaction mixture is diluted with water and extracted withEtOAc. The combined organic phase is washed with 1 N aqueous HCl, 5%aqueous NaHCO₃, water and brine, and dried over Na₂SO₄. The solvent isremoved in vacuo to afford 0.169 g (31% yield) of the desired productwhich is used without further purification.

Preparation ofN—[(S)-1-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethyl]-2-(3-methyl-1,2,4-oxadiazol-5-yl)-3-phenylpropanamide(30): Ethyl2-benzyl-3-((S)-1-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethylamino)-3-oxopropanoateis dissolved in toluene (5 mL) and heated to reflux. Potassium carbonate(80 mg) and acetamide oxime (43 mg) are added. and treated with 80 mgpotassium carbonate and 43 mg acetamide oxime at reflux. The reactionmixture is cooled to room temperature, filtered and concentrated. Theresidue is chromatographed over silica to afford 0.221 g (94%) of thedesired product as a yellow oil.

Preparation of4-{(S)-2-(4-ethylthiazol-2-yl)-2-[2-(3-methyl-1,2,4-oxadiazol-5-yl)-3-phenylpropanamido]ethyl}phenylsulfamicacid (31):N—[(S)-1-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethyl]-2-(3-methyl-1,2,4-oxadiazol-5-yl)-3-phenylpropanamide,30, (0.221 g) and tin (II) chloride (507 mg, 2.2 mmol) are dissolved inEtOH (25 mL) and the solution is brought to reflux 4 hours. The solventis removed in vacuo and the resulting residue is dissolved in EtOAc. Asaturated solution of NaHCO₃ (50 mL) is added and the solution isstirred 1 hour. The organic layer is separated and the aqueous layerextracted twice with EtOAc. The combined organic layers are dried(Na₂SO₄), filtered and concentrated to a residue which is dissolved inpyridine (0.143 g) and treated with SO₃-pyridine (0.143 g). The reactionis stirred at room temperature for 5 minutes after which a 7% solutionof NH₄OH is added. The mixture is then concentrated and the resultingresidue is purified by reverse phase chromatography to afford 0.071 g ofthe desired product as the ammonium salt. ¹H NMR (CD₃OD): δ 7.29-6.87(m, 10H), 5.38-5.30 (m, 1H), 4.37-4.30 (m, 1H), 3.42-2.74 (m, 6H),2.38-2.33 (m, 3H), 1.34-1.28 (m, 3H).

Category VI of the present disclosure relates to 2-(thiazol-2-yl)compounds having the formula:

wherein R¹, R², R³, and L are further defined herein in Table XIIIherein below.

TABLE XIII No. R² R³ R¹ M521 ethyl hydrogen thiophen-2-yl M522 ethylhydrogen thiazol-2-yl M523 ethyl hydrogen oxazol-2-yl M524 ethylhydrogen isoxazol-3-yl M525 ethyl hydrogen imidazol-2-yl M526 ethylhydrogen isoxazol-3-yl M527 ethyl hydrogen oxazol-4-yl M528 ethylhydrogen isoxazol-4-yl M529 ethyl hydrogen thiophen-4-yl M530 ethylhydrogen thiazol-4-yl M531 ethyl methyl methyl M532 ethyl methyl ethylM533 ethyl methyl propyl M534 ethyl methyl iso-propyl M535 ethyl methylbutyl M536 ethyl methyl phenyl M537 ethyl methyl benzyl M538 ethylmethyl 2-fluorophenyl M539 ethyl methyl 3-fluorophenyl M540 ethyl methyl4-fluorophenyl M541 phenyl hydrogen methyl M542 phenyl hydrogen ethylM543 phenyl hydrogen propyl M544 phenyl hydrogen iso-propyl M545 phenylhydrogen butyl M546 phenyl hydrogen phenyl M547 phenyl hydrogen benzylM548 phenyl hydrogen 2-fluorophenyl M549 phenyl hydrogen 3-fluorophenylM550 phenyl hydrogen 4-fluorophenyl M551 thiophen-2-yl hydrogen methylM552 thiophen-2-yl hydrogen ethyl M553 thiophen-2-yl hydrogen propylM554 thiophen-2-yl hydrogen iso-propyl M555 thiophen-2-yl hydrogen butylM556 thiophen-2-yl hydrogen phenyl M557 thiophen-2-yl hydrogen benzylM558 thiophen-2-yl hydrogen 2-fluorophenyl M559 thiophen-2-yl hydrogen3-fluorophenyl M560 thiophen-2-yl hydrogen 4-fluorophenyl

The compounds encompassed within Category VI of the present disclosurecan be prepared by the procedure outlined in Scheme XII and described inExample 13 herein below.

Example 13S)-4-[2-(4-Ethylthiazol-2-yl)-2-(4-oxo-4-phenylbutanamido)ethyl]-phenylsulfamicacid (33)

Preparation of (S)—N-[1-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethyl]-4-oxo-4-phenylbutanamide(32): 3-Benzoylpropionic acid (0.250 g) is dissolved in CH₂Cl₂ (5 mL),N-methyl imidazole (0.333 mL) is added and the resulting solution iscooled to 0° C. after which a solution of thionyl chloride (0.320 g) inCH₂Cl₂ (2 mL) is added dropwise. After 0.5 hours(S)-1-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethanamine, 3, (0.388 g) isadded. The reaction is stirred for 18 hours at room temperature and thenconcentrated in vacuo. The resulting residue is dissolved in EtOAc andwashed with 1N HCl and brine. The solution is dried over Na₂SO₄,filtered, and concentrated and the crude material purified over silicato afford 0.415 g of the desired product.

Preparation of(S)-4-[2-(4-ethylthiazol-2-yl)-2-(4-oxo-4-phenylbutanamido)-ethyl]phenylsulfamicacid (33):(S)—N-[1-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethyl]-2,3-diphenyl-propanamide,32, (0.2 g) is dissolved in MeOH (15 mL). A catalytic amount of Pd/C(10% w/w) is added and the mixture is stirred under a hydrogenatmosphere 18 hours. The reaction mixture is filtered through a bed ofCELITE™ and the solvent is removed under reduced pressure. The crudeproduct is dissolved in pyridine (5 mL) and treated with SO₃-pyridine(0.153 g). The reaction is stirred at room temperature for 5 minutesafter which a 7% solution of NH₄OH is added. The mixture is thenconcentrated and the resulting residue is purified by reverse phasechromatography to afford 0.090 g of the desired product as the ammoniumsalt. ¹H NMR (CD₃OD) δ 8.68 (d, 1H, J=8.2 Hz), 8.00 (d, 2H, J=7.2 Hz),7.80-7.50 (m, 3H), 7.12 (s, 4H), 7.03 (s, 1H), 5.46-5.38 (m, 1H),3.29-3.14 (m, 2H), 3.06-2.99 (m, 2H), 2.83 (q, 2H, J=7.5 Hz), 2.69-2.54(m, 2H), 1.33 (t, 3H, J=7.5 Hz).

The following are non-limiting examples of compounds encompassed withinCategory II of the present disclosure. The intermediate nitro compoundsof the following can be prepared by coupling the appropriate4-oxo-carboxcylic acid with intermediate 3 under the conditionsdescribed herein above for the formation of intermediate 4 of scheme I.

(S)-4-(2-(4-Ethylthiazol-2-yl)-2-(5-methyl-4-oxohexanamido)ethyl)phenylsulfamicacid: ¹H NMR (CD₃OD) δ 8.59 (d, 1H, J=8.1 Hz), 7.14 (s, 4H), 7.08 (t,1H, J=13.0 Hz), 5.40-5.35 (m, 1H), 3.37-3.27 (m, 2H), 3.04-2.97 (m, 1H),2.83-2.61 (m, 4H), 2.54-2.36 (m, 3H), 1.33 (t, 2H, J=7.3 Hz), 1.09 (dd,6H, J=7.0, 2.2 Hz).

(S)-4-{2-[4-(3,4-Dihydro-2H-benzo[b][1,4]dioxepin-7-yl)-4-oxobutanamido]-2-(4-ethylthiazol-2-yl)ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD) δ 8.64 (d, 1H, J=8.4 Hz), 7.60 (d, 2H, J=10.6 Hz),7.11 (s, 3H), 7.04 (d, 2H, J=5.5 Hz), 5.42-5.40 (m, 1H), 4.30-4.22 (m,4H), 3.20-2.98 (m, 4H), 2.82 (q, 2H, J=7.3 Hz), 2.67-2.48 (m, 2H), 2.23(t, 2H, J=5.5 Hz), 1.32 (t, 3H, J=7.3 Hz).

(S)-4-{2-[4-(2,3-Dimethoxyphenyl)-4-oxobutanamido]-2-(4-ethylthiazol-2-yl)ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD), δ 8.64 (?d, 1H, J=8.1 Hz), 7.21-7.11 (m, 7H), 7.02(s, 1H), 5.42 (q, 1H, J=5.9 Hz), 3.90 (d, 3H, J=3.3 Hz), 3.88 (d, 3H,J=2.9 Hz), 3.22-3.18 (m, 2H), 3.07-2.99 (m, 2H), 2.83 (q, 2H, J=7.3 Hz),2.63-2.54 (m, 2H), 1.34 (t, 3H, J=7.69 Hz).

(S)-4-{2-(4-Ethylthiazol-2-yl)-2-[4-oxo-4-(pyridin-2-yl)butanamido]ethyl}-phenylsulfamicacid: ¹H NMR (CD₃OD) δ 8.60 (d, 1H, J=12.8 Hz), 7.91-7.81 (m, 2H),7.48-7.44 (m, 1H), 7.22-7.21 (m, 1H), 6.99 (s, 3H), 6.91 (s, 1H), 5.30(q, 1H, J=5.4 Hz), 3.36 (q, 2H, J=7.0 Hz), 3.21-3.15 (m, 1H), 2.91-2.85(m, 1H), 2.74 (q, 2H, J=10.4 Hz), 2.57-2.50 (m, 2H), 1.20 (t, 3H, J=7.5Hz).

(S)-4-{2-[4-(2,3-dihydrobenzo[b][1,4]dioxin-6-yl)-4-oxobutanamido]-2-(4-ethylthiazol-2-yl)ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD) δ 7.52-7.47 (m, 2H), 7.11 (s, 4H), 7.03 (s, 1H),6.95 (d, 1H, J=8.4 Hz), 5.41 (q, 1H, J=3.7 Hz), 4.31 (d, 4H, J=5.5 Hz),3.24-3.12 (m, 2H), 3.06-2.98 (m, 2H), 2.83 (q, 2H, J=7.3 Hz), 2.62-2.53(m, 2H), 1.33 (t, 3H, J=7.3 Hz).

(S)-4-[2-(4-tert-butoxy-4-oxobutanamido)-2-(4-ethylthiazol-2-yl)ethyl]phenylsulfamicacid: ¹H NMR (CD₃OD), δ 7.10 (s 4H), 7.02 (s, 1H), 5.41 (q, 1H, J=3.7Hz), 3.30-3.25 (m, 1H), 3.06-2.99 (m, 1H), 2.83 (q, 2H, J=7.3 Hz),2.52-2.40 (m, 4H), 1.42 (s, 9H), 1.33 (t, 3H, J=7.3 Hz).

(S)-4-[2-(4-ethoxy-4-oxobutanamido)-2-(4-ethylthiazol-2-yl)ethyl]phenylsulfamicacid: ¹H NMR (CD₃OD) δ 8.62 (d, 1H, J=8.4 Hz), 7.10 (s, 4H), 7.02 (s,1H), 5.40 (q, 1H, 3.7 Hz), 4.15 (q, 2H, J=7.3 Hz), 3.28-3.25 (m, 1H),3.05-3.02 (m, 1H), 2.82 (q, 2H, J=4.4 Hz), 2.54-2.48 (m, 2H), 1.33 (t,3H, J=7.3 Hz), 1.24 (t, 3H, J=7.0 Hz).

The first aspect of Category VII of the present disclosure relates to2-(thiazol-2-yl) compounds having the formula.

wherein non-limiting examples of R¹, R², and R³ are further describedherein below in Table XIV.

TABLE XIV No. R² R³ R¹ N561 methyl hydrogen phenyl N562 methyl hydrogenbenzyl N563 methyl hydrogen 2-fluorophenyl N564 methyl hydrogen3-fluorophenyl N565 methyl hydrogen 4-fluorophenyl N566 methyl hydrogen2-chlorophenyl N567 methyl hydrogen 3-chlorophenyl N568 methyl hydrogen4-chlorophenyl N569 ethyl hydrogen phenyl N570 ethyl hydrogen benzylN571 ethyl hydrogen 2-fluorophenyl N572 ethyl hydrogen 3-fluorophenylN573 ethyl hydrogen 4-fluorophenyl N574 ethyl hydrogen 2-chlorophenylN575 ethyl hydrogen 3-chlorophenyl N576 ethyl hydrogen 4-chlorophenylN577 thiene-2-yl hydrogen phenyl N578 thiene-2-yl hydrogen benzyl N579thiene-2-yl hydrogen 2-fluorophenyl N580 thiene-2-yl hydrogen3-fluorophenyl N581 thiene-2-yl hydrogen 4-fluorophenyl N582 thiene-2-ylhydrogen 2-chlorophenyl N583 thiene-2-yl hydrogen 3-chlorophenyl N584thiene-2-yl hydrogen 4-chlorophenyl

The compounds encompassed within Category VII of the present disclosurecan be prepared by the procedure outlined in Scheme XIII and describedin Example 14 herein below.

Example 14(S)-4-(2-(3-Benzylureido)-2-(4-ethylthiazol-2-yl)ethyl)phenylsulfamicacid (35)

Preparation of(S)-1-benzyl-3-[1-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethyl]urea(34): To a solution of1-(S)-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethyl amine hydrobromide,3, (0.360 g, 1 mmol) and Et₃N (0.42 mL, 3 mmol) in 10 mL CH₂Cl₂ is addedbenzyl isocyanate (0.12 mL, 1 mmol). The mixture is stirred at roomtemperature for 18 hours. The product is isolated by filtration toafford 0.425 g (96% yield) of the desired product which is used withoutfurther purification.

Preparation of(S)-4-(2-(3-benzylureido)-2-(4-ethylthiazol-2-yl)ethyl)phenylsulfamicacid (35):(S)-1-benzyl-3-[1-(4-ethylthiazol-2-yl)-2-(4-nitrophenyl)ethyl]urea, 34,(0.425 g) is dissolved in MeOH (4 mL). A catalytic amount of Pd/C (10%w/w) is added and the mixture is stirred under a hydrogen atmosphere 18hours. The reaction mixture is filtered through a bed of CELITE™ and thesolvent is removed under reduced pressure. The crude product isdissolved in pyridine (12 mL) and treated with SO₃-pyridine (0.220 g).The reaction is stirred at room temperature for 5 minutes after which a7% solution of NH₄OH is added. The mixture is then concentrated and theresulting residue is purified by reverse phase chromatography to afford0.143 g of the desired product as the ammonium salt. ¹H NMR (CD₃OD) δ7.32-7.30 (m, 2H), 7.29-7.22 (m, 3H), 7.12-7.00 (m, 4H), 6.84 (d, 1H,J=8.1 Hz), 5.35-5.30 (m, 1H), 4.29 (s, 2H), 3.27-3.22 (m, 3H), 3.11-3.04(m, 3H), 2.81 (q, 2H, J=10.2, 13.0 Hz), 1.31 (t, 3H, J=4.5 Hz).

The following is a non-limiting examples of compounds encompassed withinthe first aspect of Category VII of the present disclosure.

4-{[(S)-2-(2-Ethylthiazol-4-yl)-2-(3-(R)-methoxy-1-oxo-3-phenylpropan-2-yl)ureido]ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD) δ 7.36-7.26 (m, 3H), 7.19-7.17 (m, 2H), 7.10-7.06(m, 2H), 6.90-6.86 (m, 3H), 5.12-5.06 (m, 1H), 4.60-4.55 (m, 1H), 3.69(s, 3H) 3.12-2.98 (m, 6H), 1.44-1.38 (m, 3H).

The second aspect of Category VII of the present disclosure relates to2-(thiazol-4-yl) compounds having the formula:

wherein non-limiting examples of R¹ and R⁴ are further described hereinbelow in Table XV.

TABLE XV No. R¹ R⁴ O585 Methyl methyl O586 Ethyl methyl O587 n-propylmethyl O588 iso-propyl methyl O589 Phenyl methyl O590 Benzyl methyl O5912-fluorophenyl methyl O592 2-chlorophenyl methyl O593 thiophen-2-ylmethyl O594 thiazol-2-yl methyl O595 oxazol-2-yl methyl O596isoxazol-3-yl methyl O597 methyl ethyl O598 ethyl ethyl O599 n-propylethyl O600 iso-propyl ethyl O601 phenyl ethyl O602 benzyl ethyl O6032-fluorophenyl ethyl O604 2-chlorophenyl ethyl O605 thiophen-2-yl ethylO606 thiazol-2-yl ethyl O607 oxazol-2-yl ethyl O608 isoxazol-3-yl ethylO609 methyl thiophen-2-yl O610 ethyl thiophen-2-yl O611 n-propylthiophen-2-yl O612 iso-propyl thiophen-2-yl O613 phenyl thiophen-2-ylO614 benzyl thiophen-2-yl O615 2-fluorophenyl thiophen-2-yl O6162-chlorophenyl thiophen-2-yl O617 thiophen-2-yl thiophen-2-yl O618thiazol-2-yl thiophen-2-yl O619 oxazol-2-yl thiophen-2-yl O620isoxazol-3-yl thiophen-2-yl O621 methyl thiazol-2-yl O622 ethylthiazol-2-yl O623 n-propyl thiazol-2-yl O624 iso-propyl thiazol-2-ylO625 phenyl thiazol-2-yl O626 benzyl thiazol-2-yl O627 2-fluorophenylthiazol-2-yl O628 2-chlorophenyl thiazol-2-yl O629 thiophen-2-ylthiazol-2-yl O630 thiazol-2-yl thiazol-2-yl O631 oxazol-2-ylthiazol-2-yl O632 isoxazol-3-yl thiazol-2-yl O633 methyl oxazol-2-ylO634 ethyl oxazol-2-yl O635 n-propyl oxazol-2-yl O636 iso-propyloxazol-2-yl O637 phenyl oxazol-2-yl O638 benzyl oxazol-2-yl O6392-fluorophenyl oxazol-2-yl O640 2-chlorophenyl oxazol-2-yl O641thiophen-2-yl oxazol-2-yl O642 thiazol-2-yl oxazol-2-yl O643 oxazol-2-yloxazol-2-yl O644 isoxazol-3-yl oxazol-2-yl

The compounds encompassed within the second aspect of Category VII ofthe present disclosure can be prepared by the procedure outlined inScheme XIV and described in Example 14 herein below.

Example 154-{(S)-2-(3-Benzylureido)-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}-phenylsulfamicacid (37)

Preparation of1-benzyl-3-{(S)-2-(4-nitrophenyl)-1-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}urea(36): To a solution of(S)-2-(4-nitrophenyl)-1-[(2-thiophen-2-yl)thiazol-4-yl)ethan-aminehydrobromide salt, 8, and Et₃N (0.42 mL, 3 mmol) in 10 mL DCM is addedbenzyl isocyanate (0.12 mL, 1 mmol). The mixture is stirred at roomtemperature for 18 hours. The product is isolated by filtration toafford 0.445 g (96% yield) of the desired product which is used withoutfurther purification.

Preparation of4-{(S)-2-(3-benzylureido)-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid (37):1-Benzyl-3-{(S)-2-(4-nitrophenyl)-1-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}urea,36, (0.445 g) is dissolved in MeOH (10 mL) and CH₂Cl₂ (5 mL). Acatalytic amount of Pd/C (10% w/w) is added and the mixture is stirredunder a hydrogen atmosphere 18 hours. The reaction mixture is filteredthrough a bed of CELITE™ and the solvent is removed under reducedpressure. The crude product is dissolved in pyridine (12 mL) and treatedwith SO₃-pyridine (0.110 g). The reaction is stirred at room temperaturefor 5 minutes after which a 7% solution of NH₄OH is added. The mixtureis then concentrated and the resulting residue is purified by reversephase chromatography to afford 0.080 g of the desired product as theammonium salt.

¹H NMR (CD₃OD) δ 7.61 (d, 1H, J=2.1 Hz), 7.58 (d, 1H, J=6 Hz), 7.33-7.22(m, 4H), 7.17-7.14 (m, 1H), 7.09-6.94 (m, 6H), 5.16 (t, 1H, J=6.6 Hz),4.13 (s, 2H), 3.14-3.11 (m, 2H).

Category VIII of the present disclosure relates to 2-(thiazol-4-yl)compounds having the formula:

wherein R¹, R⁴, and L are further defined herein in Table XVI hereinbelow.

TABLE XVI No. R⁴ L R¹ P645 methyl —SO₂— methyl P646 ethyl —SO₂— methylP647 phenyl —SO₂— methyl P648 thiophen-2-yl —SO₂— methyl P649 methyl—SO₂— trifluoromethyl P650 ethyl —SO₂— trifluoromethyl P651 phenyl —SO₂—trifluoromethyl P652 thiophen-2-yl —SO₂— trifluoromethyl P653 methyl—SO₂— ethyl P654 ethyl —SO₂— ethyl P655 phenyl —SO₂— ethyl P656thiophen-2-yl —SO₂— ethyl P657 methyl —SO₂— 2,2,2-trifluoroethyl P658ethyl —SO₂— 2,2,2-trifluoroethyl P659 phenyl —SO₂— 2,2,2-trifluoroethylP660 thiophen-2-yl —SO₂— 2,2,2-trifluoroethyl P661 methyl —SO₂— phenylP662 ethyl —SO₂— phenyl P663 phenyl —SO₂— phenyl P664 thiophen-2-yl—SO₂— phenyl P665 methyl —SO₂— 4-fluorophenyl P666 ethyl —SO₂—4-fluorophenyl P667 phenyl —SO₂— 4-fluorophenyl P668 thiophen-2-yl —SO₂—4-fluorophenyl P669 methyl —SO₂— 3,4-dihydro-2H-benzo[b][1,4]oxazin-7-yl P670 ethyl —SO₂— 3,4-dihydro-2H-benzo[b][1,4]oxazin-7-yl P671 phenyl —SO₂— 3,4-dihydro-2H-benzo[b][1,4]oxazin-7-yl P672 thiophen-2-yl —SO₂— 3,4-dihydro-2H-benzo[b][1,4]oxazin-7-yl P673 methyl —SO₂— 1-methyl-1H-imidazol-4-ylP674 ethyl —SO₂— 1-methyl-1H-imidazol-4-yl P675 phenyl —SO₂—1-methyl-1H-imidazol-4-yl P676 thiophen-2-yl —SO₂—1-methyl-1H-imidazol-4-yl P678 methyl —SO₂— 4-acetamidophenyl P679 ethyl—SO₂— 4-acetamidophenyl P680 phenyl —SO₂— 4-acetamidophenyl P681thiophen-2-yl —SO₂— 4-acetamidophenyl P682 methyl —SO₂CH₂— phenyl P683ethyl —SO₂CH₂— phenyl P684 phenyl —SO₂CH₂— phenyl P685 thiophen-2-yl—SO₂CH₂— phenyl P686 methyl —SO₂CH₂— (4-methylcarboxy- phenyl)methylP687 ethyl —SO₂CH₂— (4-methylcarboxy- phenyl)methyl P688 phenyl —SO₂CH₂—(4-methylcarboxy- phenyl)methyl P689 thiophen-2-yl —SO₂CH₂—(4-methylcarboxy- phenyl)methyl P690 methyl —SO₂CH₂—(2-methylthiazol-4-yl)methyl P691 ethyl —SO₂CH₂—(2-methylthiazol-4-yl)methyl P692 phenyl —SO₂CH₂—(2-methylthiazol-4-yl)methyl P693 thiophen-2-yl —SO₂CH₂—(2-methylthiazol-4-yl)methyl P694 methyl —SO₂CH₂CH₂— phenyl P695 ethyl—SO₂CH₂CH₂— phenyl P696 phenyl —SO₂CH₂CH₂— phenyl P697 thiophen-2-yl—SO₂CH₂CH₂— phenyl

The compounds encompassed within Category VIII of the present disclosurecan be prepared by the procedure outlined in Scheme XV and described inExample 16 herein below.

Example 16{4-(S)-[2-Phenylmethanesulfonylamino-2-(2-thiophen-2-ylthiazol-4-yl)ethyl]phenyl}sulfamicacid (39)

Preparation of(S)—N-{2-(4-nitrophenyl)-1-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}-1-phenylmethanesulfonamide(38): To a suspension of2-(4-nitrophenyl)-1-(2-thiophene2-ylthiazol-4-yl)ethylamine, 8, (330 mg,0.80 mmol) in CH₂Cl₂ (6 mL) at 0° C. is added diisopropylethylamine(0.30 mL, 1.6 mmol) followed by phenylmethanesulfonyl chloride (167 mg,0.88 mmol). The reaction mixture is stirred at room temperature for 14hours. The mixture is diluted with CH₂Cl₂ and washed with sat. NaHCO₃followed by brine, dried (Na₂SO₄), filtered and concentrated in vacuo.The resulting residue is purified over silica to afford 210 mg of thedesired product as a white solid.

Preparation of{-4-(S)-[2-phenylmethanesulfonylamino-2-(2-thiophen-2-ylthiazol-4-yl)ethyl]phenyl}sulfamicacid (39):(S)—N-{2-(4-nitrophenyl)-1-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}-1-phenylmethanesulfonamide,38, (210 mg, 0.41 mmol) is dissolved in MeOH (4 mL). A catalytic amountof Pd/C (10% w/w) is added and the mixture is stirred under a hydrogenatmosphere 18 hours. The reaction mixture is filtered through a bed ofCELITE™ and the solvent is removed under reduced pressure. The crudeproduct is dissolved in pyridine (12 mL) and treated with SO₃-pyridine(197 mg, 1.23 mmol). The reaction is stirred at room temperature for 5minutes after which a 7% solution of NH₄OH is added. The mixture is thenconcentrated and the resulting residue is purified by reverse phasechromatography to afford 0.060 g of the desired product as the ammoniumsalt. ¹H NMR (300 MHz, MeOH-d₄) δ 7.52-7.63 (m, 6.70-7.28 (m, 11H), 4.75(t, J=7.2 Hz, 1H), 3.95-4.09 (m, 2H), 3.20 (dd, J=13.5 and 7.8 Hz, 1H),3.05 (dd, J=13.5 and 7.8 Hz, 1H). 1013770

Intermediates for use in Step (a) of Scheme XV can be convenientlyprepared by the procedure outlined herein below in Scheme XVI anddescribed in Example 17.

Example 17 (2-Methylthiazol-4-yl)methanesulfonyl chloride (41)

Preparation of sodium (2-methylthiazol-4-yl)methanesulfonate (40):4-Chloromethyl-2-methylthiazole (250 mg, 1.69 mmol) is dissolved in H₂O(2 mL) and treated with sodium sulfite (224 mg, 1.78 mmol). The reactionmixture is subjected to microwave irradiation for 20 minutes at 200° C.The reaction mixture is diluted with H₂O (30 mL) and washed with EtOAc(2×25 mL). The aqueous layer is concentrated to afford 0.368 g of thedesired product as a yellow solid. LC/MS ESI+194 (M+1, free acid).

Preparation of (2-methylthiazol-4-yl)methanesulfonyl chloride (41):Sodium (2-methylthiazol-4-yl)methanesulfonate, 40, (357 mg, 1.66 mmol)is dissolved in phosphorous oxychloride (6 mL) and is treated withphosphorous pentachloride (345 mg, 1.66 mmol). The reaction mixture isstirred at 50° C. for 3 hours, then allowed to cool to room temperature.The solvent is removed under reduced pressure and the residue isre-dissolved in CH₂Cl₂ (40 mL) and is washed with sat. NaHCO₃ and brine.The organic layer is dried over MgSO₄, filtered, and the solvent removedin vacuo to afford 0.095 g of the desired product as a brown oil. LC/MSESI+211 (M+1). Intermediates are obtained in sufficient purity to becarried forward according to Scheme IX without the need for furtherpurification.

4-{(S)-2-[(2-methylthiazol-4-yl)methylsulfonamido]-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.71-7.66 (m, 2H), 7.27-7.10 (m, 7H), 4.87 (t,1H, J=7.3 Hz), 4.30-4.16 (q, 2H, J=13.2 Hz), 3.34-3.13 (m, 2H), 2.70 (s,3H).

The following are non-limiting examples of compounds encompassed withinCategory VIII of the present disclosure.

{4-(S)-[2-Phenylmethanesulfonylamino-2-(2-ethylthiazol-4-yl)ethyl]phenyl}-sulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.27-7.32 (m, 3H), 7.16-7.20 (m, 3H),7.05-7.6 (m, 2H), 6.96 (d, J=8.4 Hz, 2H), 4.70 (t, J=9.0 Hz, 1H),3.91-4.02 (m, 2H), 2.95-3.18 (m, 4H), 1.41 (t, J=7.5 Hz, 3H).

{4-(S)-[2-(3-Methoxyphenyl)methanesulfonylamino-2-(2-ethylthiazol-4-yl)ethyl]phenyl}sulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.20 (t, J=8.1 Hz. 1H), 6.94-7.08 (m,4H), 6.88-6.94 (m, 3H), 6.75-6.80 (m, 1H), 4.67 (t, J=7.2 Hz, 1H),3.90-4.0 (m, 2H), 3.76 (s, 3H), 2.95-3.16 (m, 4H), 1.40 (t, J=7.5 HZ,3H).

(S)-4-{[1-(2-Ethylthiazol-4-yl)-2-(4-sulfoaminophenyl)ethylsulfamoyl]methyl}-benzoicacid methyl ester: ¹H NMR (300 MHz, MeOH-d₄) δ 7.90-7.94-(m, 2H),7.27-7.30 (m, 2H), 7.06-7.11 (m, 3H), 6.97-7.00 (m, 2H), 4.71 (t, J=7.2Hz, 1H), 3.95-4.08 (4, 2H), 3.92 (s, 3H), 2.80-3.50 (m, 4H), 1.38-1.44(m, 3H).

(S)-4-[2-(2-Ethylthiazol-4-yl)-2-(1-methyl-1H-imidazol-4-sulfonamido)ethyl]-phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.54 (s, 1H, 7.20 (s, 1H), 7.09 (s,1H), 6.92-7.00 (m, 4H), 4.62 (t, J=5.4 Hz, 1H), 3.70 (s, 3H), 2.98-3.14(m, 3H), 2.79 (dd, J=9.3 and 15.0 Hz, 1H), 1.39 (q, J=7.5 Hz, 3H).

4-{(S)-2-[2-(Thiophen-2-yl)thiazol-4-yl]-2-(2,2,2-trifluoroethylsulfonamido)-ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.62-7.56 (m, 2H), 7.22 (s, 1H), 7.16-7.06 (m,5H), 4.84 (t, 1H, J=7.6 Hz), 3.71-3.62 (m, 2H), 3.32-3.03 (m, 2H).

{4-(S)-[2-(Phenylethanesulfonylamino)-2-(2thiophen-2-ylthiazol-4-yl)ethyl]-phenyl}sulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.56-7.62 (m, 2H), 7.04-7.19 (m, 9H),6.94-6.97 (m, 2H), 4.78 (t, J=7.8 Hz, 1H), 3.22-3.30 (m, 2H)), 3.11 (dd,J=13.5 and 7.8 Hz, 1H), 2.78-2.87 (m, 4H).

{4-(S)-[3-(Phenylpropanesulfonylamino)-2-(2thiophen-2-ylthiazol-4-yl)ethyl]-phenyl}sulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.56-7.62 (m, 2H), 6.99-7.17 (m, 10H),4.72 (t, J=7.8 Hz, 1H), 3.21 (dd, J=13.5 and 7.2 Hz, 1H), 3.02 (dd,J=13.5 and 7.2 Hz, 1H), 2.39-2.64 (m, 4H), 1.65-1.86 (m, 2H).

(S)-{4-[2-(4-Methyl-3,4-dihydro-2H-benzo[1,4]oxazine-7-sulfonylamino)-2-(2-thiophen-2-ylthiazol-4-yl)ethyl]phenyl}sulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.53 (d, J=5.1 Hz, 1H) 7.48 (d, J=5.1Hz, 1H), 7.13-7.10 (m, 1H), 7.04 (d, J=8.4 Hz, 2H), 6.93-6.88 (m, 3H),6.75 (d, J=8.1 Hz, 1H), 6.54 (d, J=8.1 Hz, 1H), 4.61 (t, J=7.5 Hz, 1H),4.20-4.08 (m, 2H), 3.14-3.00 (m, 4H), 2.69 (s, 3H).

4-{(S)-2-(4-acetamidophenylsulfonamido)-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.67-7.52 (m, 6H), 7.24-7.23 (m, 1H), 7.12-7.09(m, 3H), 7.02-6.99 (m, 2H), 4.70 (t, 1H, J=7.3 Hz), 3.25-3.00 (m, 2H),2.24 (s, 3H).

The first aspect of Category IX of the present disclosure relates tocompounds having the formula:

wherein R¹ is a substituted or unsubstituted heteroaryl and R⁴ is C₁-C₆linear, branched, or cyclic alkyl as further described herein below inTable XVII.

TABLE XVII No. R⁴ R¹ Q698 —CH₃ 4-(methoxycarbonyl)thiazol-5-yl Q699 —CH₃4-[(2-methoxy-2-oxoethyl)carbamoyl]thiazol-5-yl Q700 —CH₃5-[1-N-(2-methoxy-2-oxoethyl)-1-H-indol-3- yl]oxazol-2-yl Q701 —CH₃5-(2-methoxyphenyl)oxazol-2-yl Q702 —CH₃5-[(S)-1-(tert-butoxycarbonyl)-2- phenylethyl]oxazol-2-yl Q703 —CH₃5-[4-(methylcarboxy)phenyl]oxazol-2-yl Q704 —CH₃5-(3-methoxybenzyl)oxazol-2-yl Q705 —CH₃ 5-(4-phenyl)oxazol-2-yl Q706—CH₃ 5-(2-methoxyphenyl)thiazol-2-yl Q707 —CH₃5-(3-methoxyphenyl)thiazol-2-yl Q708 —CH₃ 5-(4-fluorophenyl)thiazol-2-ylQ709 —CH₃ 5-(2,4-difluorophenyl)thiazol-2-yl Q710 —CH₃5-(3-methoxybenzyl)thiazol-2-yl Q711 —CH₃4-(3-methoxyphenyl)thiazol-2-yl Q712 —CH₃ 4-(4-fluorophenyl)thiazol-2-ylQ713 —CH₂CH₃ 4-(methoxycarbonyl)thiazol-5-yl Q714 —CH₂CH₃4-[(2-methoxy-2-oxoethyl)carbamoyl]thiazol-5-yl Q715 —CH₂CH₃5-[1-N-(2-methoxy-2-oxoethyl)-1-H-indol-3- yl]oxazol-2-yl Q716 —CH₂CH₃5-(2-methoxyphenyl)oxazol-2-yl Q717 —CH₂CH₃5-[(S)-1-(tert-butoxycarbonyl)-2- phenylethyl]oxazol-2-yl Q718 —CH₂CH₃5-[4-(methylcarboxy)phenyl]oxazol-2-yl Q719 —CH₂CH₃5-(3-methoxybenzyl)oxazol-2-yl Q720 —CH₂CH₃ 5-(4-phenyl)oxazol-2-yl Q721—CH₂CH₃ 5-(2-methoxyphenyl)thiazol-2-yl Q722 —CH₂CH₃5-(3-methoxyphenyl)thiazol-2-yl Q723 —CH₂CH₃5-(4-fluorophenyl)thiazol-2-yl Q724 —CH₂CH₃5-(2,4-difluorophenyl)thiazol-2-yl Q725 —CH₂CH₃5-(3-methoxybenzyl)thiazol-2-yl Q726 —CH₂CH₃4-(3-methoxyphenyl)thiazol-2-yl Q727 —CH₂CH₃4-(4-fluorophenyl)thiazol-2-yl Q728 cyclopropyl4-(methoxycarbonyl)thiazol-5-yl Q729 cyclopropyl4-[(2-methoxy-2-oxoethyl)carbamoyl]thiazol-5-yl Q730 cyclopropyl5-[1-N-(2-methoxy-2-oxoethyl)-1-H-indol-3- yl]oxazol-2-yl Q731cyclopropyl 5-(2-methoxyphenyl)oxazol-2-yl Q732 cyclopropyl5-[(S)-1-(tert-butoxycarbonyl)-2- phenylethyl]oxazol-2-yl Q733cyclopropyl 5-[4-(methylcarboxy)phenyl]oxazol-2-yl Q734 cyclopropyl5-(3-methoxybenzyl)oxazol-2-yl Q735 cyclopropyl 5-(4-phenyl)oxazol-2-ylQ736 cyclopropyl 5-(2-methoxyphenyl)thiazol-2-yl Q737 cyclopropyl5-(3-methoxyphenyl)thiazol-2-yl Q738 cyclopropyl5-(4-fluorophenyl)thiazol-2-yl Q739 cyclopropyl5-(2,4-difluorophenyl)thiazol-2-yl Q740 cyclopropyl5-(3-methoxybenzyl)thiazol-2-yl Q741 cyclopropyl4-(3-methoxyphenyl)thiazol-2-yl Q742 cyclopropyl4-(4-fluorophenyl)thiazol-2-yl

Compounds according to the first aspect of Category IX which comprise asubstituted or unsubstituted thiazol-4-yl unit for R¹ can be prepared bythe procedure outlined in Scheme XVII and described herein below inExample 18.

Example 18(S)-4-(2-(2-Phenylthiazol-4-yl)2-(4-(methoxycarbonyl)thiazole-5-ylamino)ethyl)phenylsulfamicacid (45)

Preparation of (S)-2-(4-nitrophenyl)-1-(2-phenylthiazol-4-yl)ethanaminehydrobromide salt (42): A mixture of (S)-tert-butyl4-bromo-1-(4-nitrophenyl)-3-oxobutan-2-ylcarbamate, 7, (1.62 g, 4.17mmol) and thiobenzamide (0.63 g, 4.60 mmol) in CH₃CN (5 mL) is refluxedfor 24 hours. The reaction mixture is cooled to room temperature anddiethyl ether (50 mL) is added to the solution. The precipitate whichforms is collected by filtration. The solid is dried under vacuum toafford 1.2 g (67% yield) of the desired product. LC/MS ESI+326 (M+1).

Preparation of(S)-4-(1-isothiocyanato-2-(4-nitrophenyl)ethyl)-2-phenylthiazole (43):To a solution of(S)-2-(4-nitrophenyl)-1-(2-phenylthiazol-4-yl)ethanamine hydrobromidesalt, 42, (726 mg, 1.79 mmol) and CaCO₃ (716 mg, 7.16 mmol) in H₂O (2mL) is added CCl₄ (3 mL) followed by thiophosgene (0.28 mL, 3.58 mmol).The reaction is stirred at room temperature for 18 hours then dilutedwith CH₂Cl₂ and water. The layers are separated and the aqueous layerextracted with CH₂Cl₂. The combined organic layers are washed withbrine, dried (Na₂SO₄) and concentrated in vacuo to a residue which ispurified over silica (CH₂Cl₂) to afford 480 mg (73%) of the desiredproduct as a yellow solid. ¹H NMR (300 MHz, CDCl₃) δ 8.15 (d, J=8.7 Hz,2H), 7.97-7.99 (m, 2H), 7.43-7.50 (m, 3H), 7.34 (d, J=8.7 Hz, 2H), 7.15(d, J=0.9 Hz, 1H), 5.40-5.95 (m, 1H), 3.60 (dd, J=13.8 and 6.0 Hz, 1H),3.46 (dd, J=13.8 and 6.0 Hz).

Preparation of (S)-methyl5-[1-(2-phenylthiazol-4-yl)-2-(4-nitrophenyl)-ethylamino]thiazole-4-carboxylate(44): To a suspension of potassium tert-butoxide (89 mg, 0.75 mmol) inTHF (3 mL) is added methyl isocyanoacetate (65 μL, 0.68 mmol) followedby (S)-2-phenyl-4-(1-isothiocyanato-2-(4-nitrophenyl)ethyl)thiazole, 43,(250 mg, 0.68 mmol). The reaction mixture is stirred at room temperaturefor 2 hours then poured into sat. NaHCO₃. The mixture is extracted withEtOAc (3×25 mL) and the combined organic layers are washed with brineand dried (Na₂SO₄) and concentrated in vacuo. The crude residue ispurified over silica to afford 323 mg (˜100% yield) of the desiredproduct as a slightly yellow solid. ¹H NMR (300 MHz, CDCl₃) δ 8.09-8.13(m, 2H), 7.95-7.98 (m, 3H), 7.84 (d, J=1.2 Hz, 1H), 7.44-7.50 (m, 3H),7.28-7.31 (m, 2H), 7.96 (d, J=0.6 Hz, 1H), 4.71-4.78 (m, 1H), 3.92 (s,3H), 3.60 (dd, J=13.8 and 6.0 Hz, 1H), 3.45 (dd, J=13.8 and 6.0 Hz, 1H).

Preparation of(S)-4-(2-(2-phenylthiazol-4-yl)2-(4-(methoxycarbonyl)thiazole-5-ylamino)ethyl)phenylsulfamicacid (45): (S)-methyl5-[1-(2-phenylthiazol-4-yl)-2-(4-nitrophenyl)-ethylamino]thiazole-4-carboxylate,44, (323 mg, 0.68 mmol) and tin (II) chloride (612 mg, 2.72 mmol) aredissolved in EtOH and the solution is brought to reflux. The solvent isremoved in vacuo and the resulting residue is dissolved in EtOAc. Asaturated solution of NaHCO₃ is added and the solution is stirred 1hour. The organic layer is separated and the aqueous layer extractedtwice with EtOAc. The combined organic layers are dried (Na₂SO₄),filtered and concentrated to a residue which is dissolved in pyridine(10 mL) and treated with SO₃-pyridine (130 mg, 0.82 mmol). The reactionis stirred at room temperature for 5 minutes after which a 7% solutionof NH₄OH is added. The mixture is then concentrated and the resultingresidue is purified by reverse phase chromatography to afford 0.071 g ofthe desired product as the ammonium salt ¹H NMR (300 MHz, MeOH-d₄) δ7.97-8.00 (m, 3H), 7.48-7.52 (m, 3H), 7.22 (s, 1H), 7.03-7.13 (m, 4H),4.74 (t, J=6.6 Hz, 1H), 3.88 (s, 3H), 3.28-3.42 (m, 2H).

Compounds according to the first aspect of Category IX which comprise asubstituted or unsubstituted thiazol-2-yl unit for R¹ can be prepared bythe procedure outlined in Scheme XVIII and described herein below inExample 19. Intermediate 46 can be prepared according to Scheme II andExample 2 by substituting cyclopropane-carbothioic acid amide forthiophen-2-carbothioic acid amide.

Example 194-{(S)-2-(2-Cyclopropylthiazol-4-yl)-2-[4-(3-methoxyphenyl)thiazol-2-ylamino]ethyl}phenylsulfamicacid (50)

Preparation of(S)-1-(1-(2-cyclopropylthiazol-4-yl)-2-(4-nitrophenyl)ethyl)-thiourea(47): To a solution of(S)-1-(2-cyclopropylthiazol-4-yl)-2-(4-nitrophenyl)ethan-aminehydrobromide hydrobromide salt, 32, (4.04 g, 10.9 mmol) and CaCO₃ (2.18g, 21.8 mmol) in CCl₄/water (25 mL/20 mL) is added thiophosgene (1.5 g,13.1 mmol). The reaction is stirred at room temperature for 18 hoursthen diluted with CH₂Cl₂ and water. The layers are separated and theaqueous layer extracted with CH₂Cl₂. The combined organic layers arewashed with brine, dried (Na₂SO₄) and concentrated in vacuo to a residuewhich is subsequently treated with ammonia (0.5M in 1,4-dioxane, 120 mL)which is purified over silica to afford 2.90 g of the desired product asa red-brown solid. LC/MS ESI-347 (M−1).

Preparation of(S)-4-(3-methoxybenzyl)-N-(1-(2-cyclopropylthiazol-4-yl)-2-(4-nitrophenyl)ethyl)thiazol-2-amine(48):(S)-1-(1-(2-Cyclopropylthiazol-4-yl)-2-(4-nitrophenyl)ethyl)-thiourea,47, (350 mg, 1.00 mmol) and 2-bromo-3′-methoxy-acetophenone (253 mg,1.10 mmol) are combined in 3 mL CH₃CN and heated to reflux for 24 hours.The mixture is concentrated and chromatographed to afford 0.172 g of theproduct as a yellow solid. LC/MS ESI+479 (M+1).

Preparation of4-{(S)-2-(2-cyclopropylthiazol-4-yl)-2-[4-(3-methoxyphenyl)-thiazol-2-ylamino]ethyl}phenylsulfamicacid (49):(S)-4-(3-methoxybenzyl)-N-(1-(2-cyclopropylthiazol-4-yl)-2-(4-nitrophenyl)ethyl)thiazol-2-amine,48, (0.172 g) is dissolved in 10 mL MeOH. A catalytic amount of Pd/C(10% w/w) is added and the mixture is stirred under a hydrogenatmosphere for 18 hours. The reaction mixture is filtered through a bedof CELITE™ and the solvent is removed under reduced pressure. The crudeproduct is dissolved in 5 mL pyridine and treated with SO₃-pyridine (114mg). The reaction is stirred at room temperature for 5 minutes afterwhich 10 mL of a 7% solution of NH₄OH is added. The mixture is thenconcentrated and the resulting residue is purified by reverse-phasechromatography to afford 0.033 g of the desired product as the ammoniumsalt. ¹H NMR (CD₃OD): δ 7.33-7.22 (m, 3H), 7.10-6.97 (m, 5H), 6.84-6.80(m, 2H), 5.02 (t, 1H, J=6.9 Hz), 3.82 (s, 1H), 3.18 (q, 2H, J=7.1 Hz),2.36 (q, 1H, J=4.6 Hz), 1.20-1.13 (m, 2H), 1.04-0.99 (m, 2H).

The following are non-limiting examples of compounds encompassed withinthe first aspect of Category IX.

(S)-4-(2-(4-((2-Methoxy-2-oxoethyl)carbamoyl)thiazole-5-ylamino)-2-(2-ethylthiazole-4-yl)ethyl)phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.91 (s, 1H), 7.08-7.10 (m, 3H), 6.99(d, J=8.7 Hz, 2H), 4.58 (t, J=6.9 Hz, 1H), 4.11 (d, J=2.7 Hz, 2H), 3.78(s, 3H), 3.14-3.28 (m, 2H), 3.06 (q, J=7.5 Hz, 2H), 1.41 (t, J=7.5 Hz,3H).

(S)-4-(2-{5-[1-N-(2-Methoxy-2-oxoethylcarbamoyl)-1-H-indol-3-yl]oxazol-2-ylamino}-2-(2-methylthiazol-4-yl)ethyl)phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.63 (d, J=7.8 Hz, 1H), 7.37 (s, 1H),7.18-7.29 (m, 4H), 7.02-7.16 (m, 4H), 6.85 (s, 1H), 5.04-5.09 (m, 1H),4.85 (s, 3H), 3.27 (dd, J=13.5 and 8.1 Hz, 1H), 3.10 (m, J=13.5 and 8.1Hz, 1H), 2.69 (s, 3H).

4-((S)-2-(5-(2-Methoxyphenyl)oxazol-2-ylamino)-2-(2-methylthiazol-4-yl)ethyl)phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.52 (dd, J=7.5 and 1.2 Hz, 1H),6.95-7.24 (m, 10H), 5.04-5.09 (m, 1H), 3.92 (s, 3H), 3.26 (dd, J=13.8and 8.4 Hz, 1H), 3.10 (dd, J=13.8 and 8.4 Hz, 1H), 2.72 (s, 3H).

4-((S)-2-(5-((S)-1-(tert-Butoxycarbonyl)-2-phenylethyl)oxazole-2-ylamino)-2-(2-methylthiazole-4-yl)ethyl)phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.03-7.27 (m, 10H), 6.50 (s, 1H),4.95-5.00 (m, 1H), 4.76 (t, J=6.9 Hz, 1H), 3.22 (dd, J=14.1 and 6.9 Hz,1H), 3.00-3.10 (m, 2H), 2.90 (dd, J=14.1 and 6.9 Hz, 1H), 2.72 (s, 3H),1.37 (s, 9H).

(S)-{4-{2-[5-(4-Methoxycarbonyl)phenyl]oxazol-2-ylamino}-2-(2-methylthiazol-4-yl)ethyl}phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.99 (d, J=7.5 Hz, 2H), 7.56-7.59 (m,2H), 7.23-7.24 (m, 1H), 7.08-7.14 (m, 4H), 6.83 (d, J=10.2 Hz, 1H), 5.08(t, J=6.0 Hz, 1H), 3.91 (s, 3H), 3.25-3.35 (m, 1H), 3.09-3.13 (m, 1H),2.73 (s, 3H).

(S)-4-(2-(5-(3-Methoxybenzyl)oxazole-2-ylamino)-2-(2-methylthiazole-4-yl)ethyl)phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.03-7.28 (m, 8H), 6.79-6.83 (m, 1H),5.70 (s, 1H), 4.99-5.06 (m, 2H), 4.41 (d, J=2.1 Hz, 2H), 3.80 (s, 3H),3.27-3.37 (m, 1H), 3.03-3.15 (m, 1H), 2.71 (s, 3H).

(S)-4-(2-(2-Methylthiazole-4-yl)-2-(5-phenyloxazole-2-ylamino)ethyl)phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.45 (d, J=8.7 Hz, 2H), 7.33 (t, J=7.8Hz, 2H), 7.18-7.22 (m, 1H), 7.10-7.14 (m, 6H), 7.04 (s, 1H), 5.04-5.09(m, 1H), 3.26 (dd, J=13.8 and 6.3 Hz, 1H), 3.10 (dd, J=13.8 and 6.3 Hz,1H), 2.70 (s, 3H).

4-((S)-2-(2-Cyclopropylthiazol-4-yl)-2-(4-(3-methoxyphenyl)thiazol-2-ylamino)ethyl)phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.33-7.22 (m, 3H), 7.10-6.97 (m, 5H), 6.84-6.80(m, 2H), 5.02 (t, 1H, J=6.9 Hz), 3.82 (s, 1H), 3.18 (q, 2H, J=7.1 Hz),2.36 (q, 1H, J=4.6 Hz), 1.20-1.13 (m, 2H), 1.04-0.99 (m, 2H).

(S)-4-(2-(2-cyclopropylthiazol-4-yl)-2-(4-(4-fluorophenyl)thiazol-2-ylamino)ethyl)phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.79-7.74 (m, 2H), 7.14-7.03 (m, 7H), 7.21 (s,1H), 6.79 (s, 1H), 5.08 (t, 1H, J=6.6 Hz), 3.29-3.12 (m, 2H), 2.40 (q,2.40, J=5.1 Hz), 1.23-1.18 (m, 2H), 1.08-1.02 (m, 2H).

4-((S)-2-(2-cyclopropylthiazol-4-yl)-2-(4-(2-methoxyphenyl)thiazol-2-ylamino)ethyl)phenylsulfamicacid: ¹H NMR(CD₃OD): δ 7.89-7.87 (d, 1H, J=7.6 Hz), 7.28 (t, 1H, J=7.0Hz), 7.10-6.96 (m, 8H), 5.03 (t, 1H, J=6.9 Hz), 3.90 (s, 1H), 3.19 (q,2H, J=6.6 Hz), 2.38 (q, 1H, J=4.8 Hz), 1.21-1.14 (m, 2H), 1.06-1.00 (m,2H).

4-((S)-2-(2-cyclopropylthiazol-4-yl)-2-(4-(2,4-difluorophenyl)thiazol-2-ylamino)ethyl)phenylsulfamicacid: ¹H NMR(CD₃OD): δ 8.06-8.02 (q, 2H, J=6.9 Hz), 7.12-6.95 (m, 7H),6.88 (s, 1H), 5.11 (t, 1H, J=6.9 Hz), 3.22-3.15 (m, 2H), 2.38 (q, 1H,J=4.8 Hz), 1.22-1.15 (m, 2H), 1.06-1.02 (m, 2H).

(S)-4-(2-(4-(3-methoxybenzyl)thiazol-2-ylamino)-2-(2-cyclopropylthiazol-4-yl)ethyl)phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.22-7.17 (m, 3H), 7.09-6.97 (m, 5H), 6.78-6.66(m, 3H), 3.77 (s, 2H), 3.75 (s, 3H), 3.20-3.07 (m, 2H), 2.35 (q, 1H,J=4.8 Hz), 1.19-1.13 (m, 2H), 1.03-1.00 (m, 2H).

(S)-{5-[1-(2-Ethylthiazol-4-yl)-2-(4-sulfoaminophenyl)ethylamino]-2-methyl-2H-[1,2,4]triazole-3-yl}carbamic acid methyl ester: ¹H NMR (300 MHz, MeOH-d₄) δ 6.97-7.08 (m,5H), 3.71 (s, 3H), 3.51 (s, 3H), 3.15 (dd, J=13.5 and 6.3 Hz, 1H),3.02-3.07 (m, 3H), 1.40 (t, J=6.6 Hz, 3H).

The second aspect of Category V of the present disclosure relates tocompounds having the formula:

wherein R¹ is a substituted or unsubstituted heteroaryl and R⁴ issubstituted or unsubstituted phenyl and substituted or unsubstitutedheteroaryl as further described herein below in Table XVIII.

TABLE XVIII No. R⁴ R¹ R743 phenyl 4-(methoxycarbonyl)thiazol-5-yl R744phenyl 4-[(2-methoxy-2-oxoethyl)carbamoyl]thiazol-5-yl R745 phenyl5-[1-N-(2-methoxy-2-oxoethyl)-1-H-indol-3- yl]oxazol-2-yl R746 phenyl5-(2-methoxyphenyl)oxazol-2-yl R747 phenyl5-[(S)-1-(tert-butoxycarbonyl)-2- phenylethyl]oxazol-2-yl R748 phenyl5-[4-(methylcarboxy)phenyl]oxazol-2-yl R749 phenyl5-(3-methoxybenzyl)oxazol-2-yl R750 phenyl 5-(4-phenyl)oxazol-2-yl R751phenyl 5-(2-methoxyphenyl)thiazol-2-yl R752 phenyl5-(3-methoxyphenyl)thiazol-2-yl R753 phenyl5-(4-fluorophenyl)thiazol-2-yl R754 phenyl5-(2,4-difluorophenyl)thiazol-2-yl R755 phenyl5-(3-methoxybenzyl)thiazol-2-yl R756 phenyl4-(3-methoxyphenyl)thiazol-2-yl R757 phenyl4-(4-fluorophenyl)thiazol-2-yl R758 thiophen-2-yl4-(methoxycarbonyl)thiazol-5-yl R759 thiophen-2-yl4-[(2-methoxy-2-oxoethyl)carbamoyl]thiazol-5-yl R760 thiophen-2-yl5-[1-N-(2-methoxy-2-oxoethyl)-1-H- indol-3-yl]oxazol-2-yl R761thiophen-2-yl 5-(2-methoxyphenyl)oxazol-2-yl R762 thiophen-2-yl5-[(S)-1-(tert-butoxycarbonyl)-2- phenylethyl]oxazol-2-yl R763thiophen-2-yl 5-[4-(methylcarboxy)phenyl]oxazol-2-yl R764 thiophen-2-yl5-(3-methoxybenzyl)oxazol-2-yl R765 thiophen-2-yl5-(4-phenyl)oxazol-2-yl R766 thiophen-2-yl5-(2-methoxyphenyl)thiazol-2-yl R767 thiophen-2-yl5-(3-methoxyphenyl)thiazol-2-yl R768 thiophen-2-yl5-(4-fluorophenyl)thiazol-2-yl R769 thiophen-2-yl5-(2,4-difluorophenyl)thiazol-2-yl R770 thiophen-2-yl5-(3-methoxybenzyl)thiazol-2-yl R771 thiophen-2-yl4-(3-methoxyphenyl)thiazol-2-yl R772 thiophen-2-yl4-(4-fluorophenyl)thiazol-2-yl R773 cyclopropyl4-(methoxycarbonyl)thiazol-5-yl R774 cyclopropyl4-[(2-methoxy-2-oxoethyl)carbamoyl]thiazol-5-yl R775 cyclopropyl5-[1-N-(2-methoxy-2-oxoethyl)-1-H-indol-3- yl]oxazol-2-yl R776cyclopropyl 5-(2-methoxyphenyl)oxazol-2-yl R777 cyclopropyl5-[(S)-1-(tert-butoxycarbonyl)-2- phenylethyl]oxazol-2-yl R778cyclopropyl 5-[4-(methylcarboxy)phenyl]oxazol-2-yl R779 cyclopropyl5-(3-methoxybenzyl)oxazol-2-yl R780 cyclopropyl 5-(4-phenyl)oxazol-2-ylR781 cyclopropyl 5-(2-methoxyphenyl)thiazol-2-yl R782 cyclopropyl5-(3-methoxyphenyl)thiazol-2-yl R783 cyclopropyl5-(4-fluorophenyl)thiazol-2-yl R784 cyclopropyl5-(2,4-difluorophenyl)thiazol-2-yl R785 cyclopropyl5-(3-methoxybenzyl)thiazol-2-yl R786 cyclopropyl4-(3-methoxyphenyl)thiazol-2-yl R787 cyclopropyl4-(4-fluorophenyl)thiazol-2-yl

Compounds according to the second aspect of Category IX which comprise asubstituted or unsubstituted thiazol-4-yl unit for R¹ can be prepared bythe procedure outlined in Schemes XIX, XX, and XXI and described hereinbelow in Examples 20, 21, and 22.

Example 20(S)-4-(2-(5-Methyl-1,3,4-thiadiazol-2-ylamino)-2-(2-phenylthiazol-4-yl)ethyl)phenylsulfamicacid (55)

Preparation of [3-diazo-1-(4-nitrobenzyl)-2-oxo-propyl]-carbamic acidtert-butyl ester (50): To a 0° C. solution of2-(S)-tert-butoxycarbonylamino-3-(4-nitrophenyl)-propionic acid (1.20 g,4.0 mmol) in THF (20 mL) is added dropwise triethylamine (0.61 mL, 4.4mmol) followed by iso-butyl chloroformate (0.57 mL, 4.4 mmol). Thereaction mixture is stirred at 0° C. for 20 minutes then filtered. Thefiltrate is treated with an ether solution of diazomethane (˜16 mmol) at0° C. The reaction mixture is stirred at room temperature for 3 hoursand concentrated. The residue is dissolved in EtOAc and washedsuccessively with water and brine, dried (Na₂SO₄), filtered andconcentrated in vacuo. The resulting residue is purified over silica(hexane/EtOAc 2:1) to afford 1.1 g (82% yield) of the desired product asa slightly yellow solid. ¹H NMR (300 MHz, CDCl₃) δ 8.16 (d, J=8.7 Hz,2H), 7.39 (d, J=8.7 Hz, 2H), 5.39 (s, 1H), 5.16 (d, J=6.3 Hz, 1H), 4.49(s, 1H), 3.25 (dd, J=13.8 and 6.6, 1H), 3.06 (dd, J=13.5 and 6.9 Hz,1H), 1.41 (s, 9H).

Preparation of [3-bromo-1-(4-nitro-benzyl)-2-oxo-propyl]-carbamic acidtert-butyl ester (51): To a 0° C. solution of[3-diazo-1-(4-nitrobenzyl)-2-oxo-propyl]-carbamic acid tert-butyl ester,50, (0.350 g, 1.04 mmol) in THF (5 mL) is added dropwise 48% aq. HBr(0.14 mL, 1.25 mmol). The reaction mixture is stirred at 0° C. for 1.5hours and quenched at 0° C. with saturated aqueous Na₂CO₃. The mixtureis extracted with EtOAc (3×25 mL) and the combined organic extracts arewashed with brine, dried (Na₂SO₄), filtered and concentrated in vacuo toafford 0.400 g of the desired product that is used in the next stepwithout further purification. ¹H NMR (300 MHz, CDCl₃) δ 8.20 (d, J=8.4Hz, 2H), 7.39 (d, J=8.4 Hz, 2H), 5.06 (d, J=7.8 Hz, 1H), 4.80 (q, J=6.3Hz, 1H), 4.04 (s, 2H), 1.42 (s, 9H).

Preparation of (S)-2-(4-nitrophenyl)-1-(2-phenylthiazol-4-yl)ethanaminehydrobromide salt (52): A mixture of[3-bromo-1-(4-nitro-benzyl)-2-oxo-propyl]-carbamic acid tert-butylester, 51, (1.62 g, 4.17 mmol) and benzothioamide (0.630 g, 4.59 mmol),in CH₃CN (5 mL) is refluxed for 24 hours. The reaction mixture is cooledto room temperature and diethyl ether (50 mL) is added to the solutionand the precipitate that forms is collected by filtration. The solid isdried under vacuum to afford 1.059 g (63%) of the desired product.ESI+MS 326 (M+1).

Preparation of(S)-4-[1-isothiocyanato-2-(4-nitrophenyl)-ethyl]-2-phenylthiazole (53):To a solution of(S)-2-(4-nitrophenyl)-1-(2-phenylthiazol-4-yl)ethanamine hydrobromidesalt, 52, (2.03 g, 5 mmol) and CaCO₃ (1 g, 10 mmol) in CCl₄/water(10:7.5 mL) is added thiophosgene (0.46 mL, 6 mmol). The reaction isstirred at room temperature for 18 hours then diluted with CH₂Cl₂ andwater. The layers are separated and the aqueous layer extracted withCH₂Cl₂. The combined organic layers are washed with brine, dried(Na₂SO₄) and concentrated in vacuo to a residue that is purified oversilica (CH₂Cl₂) to afford 1.71 g (93% yield) of the desired product.ESI+MS 368 (M+1).

Preparation of(S)-5-methyl-N-[2-(4-nitrophenyl)-1-(2-phenylthiazol-4-yl)ethyl]-1,3,4-thiadiazol-2-amine(54): A solution of(S)-4-[1-isothiocyanato-2-(4-nitrophenyl)-ethyl]-2-phenylthiazole, 53,(332 mg, 0.876 mmol) and acetic hydrazide (65 mg, 0.876 mmol) in EtOH (5mL) is refluxed for 2 hours. The solvent is removed under reducedpressure, the residue is dissolved in POCl₃ (3 mL) and the resultingsolution is stirred at room temperature for 18 hours after which thesolution is heated to 50° C. for 2 hours. The solvent is removed invacuo and the residue is dissolved in EtOAc (40 mL) and the resultingsolution is treated with 1N NaOH until the pH remains approximately 8.The solution is extracted with EtOAc. The combined aqueous layers arewashed with EtOAc, the organic layers combined, washed with brine, driedover MgSO₄, filtered, and concentrated in vacuo to afford 0.345 g (93%yield) of the desired product as a yellow solid. ¹H NMR (CDCl₃) 8.09 (d,J=8.4 Hz, 2H), 7.91 (m, 2H), 7.46 (m, 4H), 7.44 (s, 1H), 5.23 (m, 1H),3.59 (m, 2H), 2.49 (s, 3H). ESI+MS 424 (M+1).

Preparation of(S)-4-[2-(5-methyl-1,3,4-thiadiazol-2-ylamino)-2-(2-phenylthiazol-4-yl)ethyl]phenylsulfamicacid (55):(S)-5-Methyl-N-[2-(4-nitrophenyl)-1-(2-phenylthiazol-4-yl)ethyl]-1,3,4-thiadiazol-2-amine,54, (0.404 g, 0.954 mmol) is dissolved in MeOH (5 mL). Pd/C (50 mg, 10%w/w) is added and the mixture is stirred under a hydrogen atmosphereuntil the reaction is judged to be complete. The reaction mixture isfiltered through a bed of CELITE™ and the solvent removed under reducedpressure. The crude product is dissolved in pyridine (4 mL) and treatedwith SO₃-pyridine (0.304 g, 1.91 mmol). The reaction is stirred at roomtemperature for 5 minutes after which a 7% solution of NH₄OH (50 mL) isadded. The mixture is then concentrated and the resulting residue ispurified by reverse phase preparative HPLC to afford 0.052 g (11% yield)of the desired product as the ammonium salt. ¹H NMR (CD₃OD): δ 8.00-7.97(m, 2H), 7.51-7.47 (m, 3H), 7.23 (s, 1H), 7.11-7.04 (q, 4H, J=9.0 Hz),5.18 (t, 1H, J=7.2 Hz), 3.34-3.22 (m, 2H), 2.50 (s, 3H). ESI− MS 472(M−1).

Example 214-{(S)-2-[4-(2-Methoxyphenyl)thiazol-2-ylamino)-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid (58)

Preparation of(S)-1-[1-(thiophen-2-ylthiazol-4-yl)-2-(4-nitrophenyl)ethyl]-thiourea(56): To a solution of(S)-2-(4-nitrophenyl)-1-(thiophen-2-ylthiazol-4-yl)ethanaminehydrobromide salt, 8, (1.23 g, 2.98 mmol) and CaCO₃ (0.597 g, 5.96 mmol)in CCl₄/water (10 mL/5 mL) is added thiophosgene (0.412 g, 3.58 mmol).The reaction is stirred at room temperature for 18 hours then dilutedwith CH₂Cl₂ and water. The layers are separated and the aqueous layerextracted with CH₂Cl₂. The combined organic layers are washed withbrine, dried (Na₂SO₄) and concentrated in vacuo to a residue which issubsequently treated with ammonia (0.5M in 1,4-dioxane, 29.4 mL, 14.7mmol) which is purified over silica to afford 0.490 g of the desiredproduct as a red-brown solid. ESI+MS 399 (M+1).

Preparation of4-(2-methoxyphenyl)-N-{(S)-2-(4-nitrophenyl)-1-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}thiazol-2-amine(57):(S)-1-[1-(thiophen-2-ylthiazol-4-yl)-2-(4-nitrophenyl)ethyl]-thiourea,56, (265 mg, 0.679 mmol) is treated with bromo-2′-methoxyacetophenone(171 mg, 0.746 mmol) to afford 0.221 g of the product as a yellow solid.ESI+MS 521 (M+1).

Preparation on4-{(S)-2-[4-(2-methoxyphenyl)thiazol-2-ylamino)-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid (58):4-(2-methoxyphenyl)-N-{(S)-2-(4-nitrophenyl)-1-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}thiazol-2-amine,57, (0.229 g) is dissolved in 12 mL MeOH. A catalytic amount of Pd/C(10% w/w) is added and the mixture is stirred under a hydrogenatmosphere for 18 hours. The reaction mixture is filtered through a bedof CELITE™ and the solvent is removed under reduced pressure. The crudeproduct is dissolved in 6 mL pyridine and treated with SO₃-pyridine (140mg). The reaction is stirred at room temperature for 5 minutes afterwhich 10 mL of a 7% solution of NH₄OH is added. The mixture is thenconcentrated and the resulting residue is purified by reverse-phasechromatography to afford 0.033 g of the desired product as the ammoniumsalt. ¹H MR(CD₃OD): δ 7.96-7.93 (m, 1H), 7.60-7.55 (m, 2H), 7.29-7.23(m, 1H), 7.18-6.95 (m, 9H), 5.15 (t, 1H, J=6.9 Hz), 3.90 (s, 3H),3.35-3.24 (m, 2H).

Compounds according to the second aspect of Category IX which comprise asubstituted or unsubstituted oxazol-2-yl unit for R¹ can be prepared bythe procedure outlined in Scheme XXI and described herein below inExample 22. Intermediate 39 can be prepared according to Scheme XVII andExample 18.

Example 224-{(S)-2-[5-(3-Methoxyphenyl)oxazole-2-ylamino]-2-(2-phenylthiazole-4-yl)ethyl}phenylsulfamicacid (61)

Preparation of[5-(3-methoxyphenyl)oxazol-2-yl]-[2-(4-nitrophenyl)-1-(2-phenylthiazole-4-yl)ethyl]amine(60): A mixture of(S)-4-(isothiocyanato-2-(4-nitrophenyl)ethyl)-2-phenylthiazole, 53, (300mg, 0.81 mmol), 1-azido-1-(3-methoxyphenyl)ethanone (382 mg, 2.0 mmol)and PPh₃ (0.8 g, polymer bound, ˜3 mmol/g) in dioxane (6 mL) is heatedat 90° C. for 20 minutes. The reaction solution is cooled to roomtemperature and the solvent removed in vacuo and the resulting residueis purified over silica to afford 300 mg (74% yield) of the desiredproduct as a yellow solid. ¹H NMR (300 MHz, MeOH-d₄) δ 8.02 (d, J=7.2Hz, 2H), 7.92-7.99 (m, 2H), 7.42-7.47 (m, 3H), 7.22-7.27 (m, 3H),6.69-7.03 (m, 4H), 6.75-6.78 (m, 1H), 5.26 (t, J=6.3 Hz, 1H), 3.83 (s,4H), 3.42-3.45 (m, 2H).

Preparation of4-{(S)-2-[5-(3-methoxyphenyl)oxazole-2-ylamino]-2-(2-phenylthiazole-4-yl)ethyl}phenylsulfamicacid (61):[5-(3-methoxyphenyl)oxazol-2-yl]-[2-(4-nitrophenyl)-1-(2-phenylthiazole-4-yl)ethyl]amine,60, (300 mg, 0.60 mmol) is dissolved in MeOH (15 mL). A catalytic amountof Pd/C (10% w/w) is added and the mixture is stirred under a hydrogenatmosphere 18 hours. The reaction mixture is filtered through a bed ofCELITE™ and the solvent is removed under reduced pressure. The crudeproduct is dissolved in pyridine (10 mL) and treated with SO₃-pyridine(190 mg, 1.2 mmol). The reaction is stirred at room temperature for 5minutes after which a 7% solution of NH₄OH is added. The mixture is thenconcentrated and the resulting residue is purified by reverse-phasechromatography to afford 0.042 g of the desired product as the ammoniumsalt. ¹H NMR (300 MHz, MeOH-d₄) δ 7.99 (d, J=7.5 Hz, 2H), 7.46-7.50 (m,3H), 7.23-7.29 (m, 3H), 7.04-7.12 (m, 6H), 6.78 (dd, J=8.4 and 2.4 Hz,1H), 5.16 (t, J=6.6 Hz, 1H), 3.81 (s, 3H), 3.29-3.39 (m, 1H), 3.17 (dd,J=13.8 and 8.1 Hz, 1H).

The following are non-limiting examples of the second aspect of CategoryIX of the present disclosure.

(S)-4-(2-(5-Phenyl-1,3,4-thiadiazol-2-ylamino)-2-(2-phenylthiazol-4-yl)ethyl)-phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.97-7.94 (m, 2H), 7.73-7.70 (m, 2H), 7.44-7.39(m, 6H), 7.25 (s, 1H), 7.12 (s, 4H), 5.29 (t, 1H, J=6.9 Hz), 3.35-3.26(m, 2H).

4-((S)-2-(5-Propyl-1,3,4-thiadiazol-2-ylamino)-2-(2-(thiophen-2-yl)thiazol-4-yl)ethyl)phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.59-7.54 (m, 2H), 7.17-7.03 (m, 6H), 5.13 (t,1H, J=7.2 Hz), 3.32-3.13 (m, 2H), 2.81 (t, 2H, J=7.4 Hz), 1.76-1.63 (h,6H, J=7.4 Hz), 0.97 (t, 3H, J=7.3 Hz).

4-((S)-2-(5-Benzyl-1,3,4-thiadiazol-2-ylamino)-2-(2-(thiophen-2-yl)thiazol-4-yl)ethyl)phenylsulfamicacid: ¹H NMR (CD₃OD): δ (m, 2H), 7.49-7.45 (m, 2H), 7.26-7.16 (m, 5H),7.05-6.94 (m, 6H), 5.04 (t, 1H, J=7.1 Hz), 4.07 (s, 2H), 3.22-3.04 (m,2H).

4-((S)-2-(5-(Naphthalen-1-ylmethyl)-1,3,4-thiadiazol-2-ylamino)-2-(2-(thiophen-2-yl)thiazol-4-yl)ethyl)phenylsulfamicacid: ¹H NMR (CD₃OD): δ 8.08-8.05 (m, 1H), 7.89-7.80 (m, 2H), 7.55-7.43(m, 6H), 7.11-7.00 (m, 6H), 5.08 (t, 1H, J=7.1 Hz), 4.63 (s, 2H),3.26-3.08 (m, 2H).

4-((S)-2-(5-((Methoxycarbonyl)methyl)-1,3,4-thiadiazol-2-ylamino)-2-(2-(thiophen-2-yl)thiazol-4-yl)ethyl)phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.48-7.44 (m, 2H), 7.03-6.92 (m, 6H), 5.02 (t,1H, J=7.2 Hz), 4.30 (s, 2H), 3.55 (s, 3H), 3.22-3.02 (m, 2H).

4-((S)-2-(5-((2-Methylthiazol-4-yl)methyl)-1,3,4-thiadiazol-2-ylamino)-2-(2-(thiophen-2-yl)thiazol-4-yl)ethyl)phenylsulfamicacid: ¹H(CD₃OD): δ 7.60-7.56 (m, 2H), 7.19 (s, 1H), 7.15-7.12 (m, 2H),7.09-7.03 (q, 4H, J=8.7 Hz), 5.14 (t, 1H, J=7.2 Hz), 4.28 (s, 2H),3.33-3.14 (m, 2H), 2.67 (s, 3H).

4-{(S)-2-[4-(2,4-Difluorophenyl)thiazol-2-ylamino]-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD): δ 8.06-8.02 (q, 1H, J=6.8 Hz), 7.59-7.54 (m, 2H),7.16-7.08 (m, 6H), 7.01-6.88 (m, 4H), 5.20 (t, 1H, J=7.0 Hz), 3.36-3.17(m, 2H).

(S)-4-{2-[4-(Ethoxycarbonyl)thiazol-2-ylamino]-2-(2-phenylthiazol-4-yl)ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD): δ 8.02-7.99 (m, 2H), 7.54-7.45 (m, 4H), 7.26 (s,1H), 7.08 (s, 4H), 5.26 (t, 1H, J=6.9 Hz), 4.35-4.28 (q, 2H, J=6.9 Hz),3.38-3.18 (m, 2H), 1.36 (t, 3H, J=7.2 Hz).

(S)-4-{2-[4-(2-Ethoxy-2-oxoethyl)thiazol-2-ylamino]-2-(2-phenylthiazol-4-yl)ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.96 (m, 2H), 7.50-7.46 (m, 3H), 7.21 (s, 1H),7.10-7.04 (m, 4H), 6.37 (s, 1H), 5.09 (t, 1H, J=6.9 Hz), 4.17-4.10 (q,2H, J=7.1 Hz), 3.54 (s, 2H), 3.35-3.14 (m, 2H), 1.22 (t, 3H, J=7.1 Hz).

(S)-4-{2-[4-(4-acetamidophenyl)thiazol-2-ylamino]-2-(2-phenylthiazol-4-yl)ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD): δ 8.11 (m, 2H), 7.82-7.80 (m, 2H), 7.71-7.61 (m,6H), 7.40 (s, 1H), 7.23 (s, 4H), 5.32 (t, 1H, J=7.0 Hz), 3.51-3.35 (m,2H), 2.28 (s, 3H).

(S)-4-[2-(4-phenylthiazol-2-ylamino)-2-(2-phenylthiazol-4-yl)ethyl]phenylsulfamicacid: ¹H (CD₃OD): δ 8.03-7.99 (m, 2H), 7.75-7.72 (d, 2H, J=8.4 Hz),7.53-7.48 (m, 3H), 7.42 (m, 4H), 7.12 (s, 4H), 6.86 (s, 1H), 5.23 (t,1H, J=7.2 Hz), 3.40-3.27 (m, 2H).

(S)-4-{2-[4-(4-(methoxycarbonyl)phenyl)thiazol-2-ylamino]-2-(2-phenylthiazol-4-yl)ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD): δ 8.04-8.00 (m, 4H), 7.92-7.89 (d, 2H, J=9.0 Hz),7.53-7.49 (m, 3H), 7.30 (s, 1H), 7.15 (s, 4H), 7.05 (s, 1H), 5.28 (t,1H, J=6.9 Hz), 3.93 (s, 3H), 3.35-3.24 (m, 2H).

4-{(S)-2-[4-(Ethoxycarbonyl)thiazol-2-ylamino]-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid: ¹H NMR (CD₃OD): δ 7.43-7.38 (m, 2H), 7.26 (s, 1H), 7.00-6.94 (m,3H), 6.89 (s, 4H), 5.02 (t, 1H, J=7.0 Hz), 4.16-4.09 (q, 2H, J=7.1 Hz),3.14-2.94 (m, 2H),

(S)-4-[2-(4-(Methoxycarbonyl)thiazol-5-ylamino)-2-(2-phenylthiazole-4-yl)ethyl]phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.97-8.00 (m, 3H), 7.48-7.52 (m, 3H),7.22 (s, 1H), 7.03-7.13 (m, 4H), 4.74 (t, J=6.6 Hz, 1H), 3.88 (s, 3H),3.28-3.42 (m, 2H).

(S)-4-[2-(5-Phenyloxazol-2-ylamino)-2-(2-phenylthiazol-4-yl)ethyl]-phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.94-7.96 (m, 2H), 7.45-7.49 (m, 5H),7.32 (t, J=7.8 Hz, 2H), 7.12 (s, 1H), 7.19 (t, J=7.2 Hz, 1H), 7.12 (s,4H), 7.05 (s, 1H), 5.15 (t, J=6.4 Hz, 1H), 3.34 (dd, J=14.1 and 8.4 Hz,1H), 3.18 (dd, J=14.1 and 8.4 Hz, 1H).

(S)-4-{2-[5-(4-Acetamidophenyl)oxazol-2-ylamino]-2-(2-phenylthiazol-4-yl)ethyl}phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.92-7.94 (m, 2H), 7.55-7.58 (m, 2H),7.39-7.50 (m, 5H), 7.26 (s, 1H), 7.12 (s, 4H), 7.02 (s, 1H0), 5.14 (t,J=7.8 Hz, 1H), 3.13-3.38 (m, 2H), 2.11 (s, 3H).

4-((S)-2-(5-(2,4-Difluorophenyl)oxazole-2-ylamino)-2-(2-phenylthiazole-4-yl)ethyl)phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.97-7.99 (m, 2H), 7.54-7.62 (m, 1H),7.45-7.50 (m, 3H), 7.28 (s, 1H), 7.12 (s, 4H), 6.97-7.06 (m, 3H),5.15-5.20 (m, 1H), 3.28-3.40 (m, 1H), 3.20 (dd, J=13.8 and 8.4 Hz, 1H).

4-{(S)-2-[5-(3-Methoxyphenyl)oxazol-2-ylamino]-2-[(2-thiophen-2-yl)thiazole-4-yl]ethyl}phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.55-7.60 (m, 2H), 7.26 (t, J=8.1 Hz,1H), 7.21 (s, 1H), 7.04-7.15 (m, 8H), 6.77-6.81 (m, 1H), 5.10 (t, J=6.3Hz, 1H), 3.81 (s, 3H), 3.29-3.36 (m, 1H), 3.15 (dd, J=14.1 and 8.4 Hz,1H).

(S)-4-[2-(4,6-Dimethylpyrimidin-2-ylamino)-2-(2-methylthiazole-4-yl)ethyl]phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d₄) δ 7.00-7.10 (m, 5H), 6.44 (s, 1H), 5.50(t, J=7.2 Hz, 1H), 3.04-3.22 (m, 2H), 2.73 (s, 3H), 2.27 (s, 6H).

(S)-4-[2-(4-Hydroxy-6-methylpyrimidine-2-ylamino)-2-(2-methylthiazole-4-yl)ethyl]phenylsulfamicacid: ¹H NMR (300 MHz, MeOH-d4) δ 7.44 (d, J=8.4 Hz, 2H), 6.97-7.10 (m,4H), 5.61 (s, 1H), 5.40-5.49 (m, 1H), 3.10-3.22 (m, 2H), 2.73 (s, 3H),2.13 (s, 3H).

The first aspect of Category X of the present disclosure relates tocompounds having the formula:

wherein R¹ is heteroaryl and R⁴ is further described herein below inTable XIX.

TABLE XIX No. R⁴ R¹ S788 phenyl 4-(methoxycarbonyl)thiazol-5-yl S789phenyl 4-[(2-methoxy-2-oxoethyl)carbamoyl]thiazol- 5-yl S790 phenyl5-[1-N-(2-methoxy-2-oxoethyl)-1-H-indol-3- yl]oxazol-2-yl S791 phenyl5-(2-methoxyphenyl)oxazol-2-yl S792 phenyl5-[(S)-1-(tert-butoxycarbonyl)-2- phenylethyl]oxazol-2-yl S793 phenyl5-[4-(methylcarboxy)phenyl]oxazol-2-yl S794 phenyl5-(3-methoxybenzyl)oxazol-2-yl S795 phenyl 5-(4-phenyl)oxazol-2-yl S796phenyl 5-(2-methoxyphenyl)thiazol-2-yl S797 phenyl5-(3-methoxyphenyl)thiazol-2-yl S798 phenyl5-(4-fluorophenyl)thiazol-2-yl S799 phenyl5-(2,4-difluorophenyl)thiazol-2-yl S800 phenyl5-(3-methoxybenzyl)thiazol-2-yl S801 phenyl4-(3-methoxyphenyl)thiazol-2-yl S802 phenyl4-(4-fluorophenyl)thiazol-2-yl S803 thiophen-2-yl4-(methoxycarbonyl)thiazol-5-yl S804 thiophen-2-yl4-[(2-methoxy-2-oxoethyl)carbamoyl]thiazol- 5-yl S805 thiophen-2-yl5-[1-N-(2-methoxy-2-oxoethyl)-1-H- indol-3-yl]oxazol-2-yl S806thiophen-2-yl 5-(2-methoxyphenyl)oxazol-2-yl S8007 thiophen-2-yl5-[(S)-1-(tert-butoxycarbonyl)-2- phenylethyl]oxazol-2-yl S808thiophen-2-yl 5-[4-(methylcarboxy)phenyl]oxazol-2-yl S809 thiophen-2-yl5-(3-methoxybenzyl)oxazol-2-yl S810 thiophen-2-yl5-(4-phenyl)oxazol-2-yl S811 thiophen-2-yl5-(2-methoxyphenyl)thiazol-2-yl S812 thiophen-2-yl5-(3-methoxyphenyl)thiazol-2-yl S813 thiophen-2-yl5-(4-fluorophenyl)thiazol-2-yl S814 thiophen-2-yl5-(2,4-difluorophenyl)thiazol-2-yl S815 thiophen-2-yl5-(3-methoxybenzyl)thiazol-2-yl S816 thiophen-2-yl4-(3-methoxyphenyl)thiazol-2-yl S817 thiophen-2-yl4-(4-fluorophenyl)thiazol-2-yl S818 cyclopropyl4-(methoxycarbonyl)thiazol-5-yl S819 cyclopropyl4-[(2-methoxy-2-oxoethyl)carbamoyl]thiazol- 5-yl S820 cyclopropyl5-[1-N-(2-methoxy-2-oxoethyl)-1-H-indol-3- yl]oxazol-2-yl S821cyclopropyl 5-(2-methoxyphenyl)oxazol-2-yl S822 cyclopropyl5-[(S)-1-(tert-butoxycarbonyl)-2- phenylethyl]oxazol-2-yl S823cyclopropyl 5-[4-(methylcarboxy)phenyl]oxazol-2-yl S824 cyclopropyl5-(3-methoxybenzyl)oxazol-2-yl S825 cyclopropyl 5-(4-phenyl)oxazol-2-ylS826 cyclopropyl 5-(2-methoxyphenyl)thiazol-2-yl S827 cyclopropyl5-(3-methoxyphenyl)thiazol-2-yl S828 cyclopropyl5-(4-fluorophenyl)thiazol-2-yl S829 cyclopropyl5-(2,4-difluorophenyl)thiazol-2-yl S830 cyclopropyl5-(3-methoxybenzyl)thiazol-2-yl S831 cyclopropyl4-(3-methoxyphenyl)thiazol-2-yl S832 cyclopropyl4-(4-fluorophenyl)thiazol-2-yl

Compounds according to the first aspect of Category X can be prepared bythe procedure outlined in Scheme XXII and described herein below inExample 23.

Example 234-((S)-2-(2-(3-Chlorophenyl)acetamido)-2-(2-(thiophen-2-yl)oxazol-4-yl)ethyl)phenylsulfamicacid (64)

Preparation of(S)-2-(4-nitrophenyl)-1-[(thiophen-2-yl)oxazol-4-yl]ethanaminehydrobromide salt (62): A mixture of (S)-tert-butyl4-bromo-1-(4-nitrophenyl)-3-oxobutan-2-ylcarbamate, 7, (38.7 g, 100mmol), and thiophen-2-carboxamide (14 g, 110 mmol) (available from AlfaAesar) in CH₃CN (500 mL) is refluxed for 5 hours. The reaction mixtureis cooled to room temperature and diethyl ether (200 mL) is added to thesolution. The precipitate which forms is collected by filtration. Thesolid is dried under vacuum to afford the desired product which can beused for the next step without purification.

Preparation of2-(3-chlorophenyl)-N-{(S)-2-(4-nitrophenyl)-1-[2-(thiophen-2-yl)oxazol-4-yl]ethyl}acetamide(63): To a solution of(S)-2-(4-nitrophenyl)-1-[(thiophen-2-yl)oxazol-4-yl]ethanamine HBr, 47,(3.15 g, 10 mmol) 3-chlorophenyl-acetic acid (1.70 g, 10 mmol) and1-hydroxybenzotriazole (HOBt) (0.70 g, 5.0 mmol) in DMF (50 mL) at 0°C., is added 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDCI) (1.90g, 10 mmol) followed by triethylamine (4.2 mL, 30 mmol). The mixture isstirred at 0° C. for 30 minutes then at room temperature overnight. Thereaction mixture is diluted with water and extracted with EtOAc. Thecombined organic phase is washed with 1 N aqueous HCl, 5% aqueousNaHCO₃, water and brine, and dried over Na₂SO₄. The solvent is removedin vacuo to afford the desired product which is used without furtherpurification.

Preparation of—((S)-2-(2-(3-chlorophenyl)acetamido)-2-(2-(thiophen-2-yl)oxazol-4-yl)ethyl)phenylsulfamicacid (64):2-(3-chlorophenyl)-N-{(S)-2-(4-nitrophenyl)-1-[2-(thiophen-2-yl)oxazol-4-yl]ethyl}acetamide,63, (3 g) is dissolved in MeOH (4 mL). A catalytic amount of Pd/C (10%w/w) is added and the mixture is stirred under a hydrogen atmosphere 18hours. The reaction mixture is filtered through a bed of CELITE™ and thesolvent is removed under reduced pressure. The crude product isdissolved in pyridine (12 mL) and treated with SO₃-pyridine (0.157 g).The reaction is stirred at room temperature for 5 minutes after which a7% solution of NH₄OH is added. The mixture is then concentrated and theresulting residue can be purified by reverse phase chromatography toafford the desired product as the ammonium salt.

The second aspect of Category X of the present disclosure relates tocompounds having the formula:

wherein R¹ is aryl and R² and R³ are further described herein below inTable XX.

TABLE XX No. R² R³ R¹ T833 methyl hydrogen phenyl T834 methyl hydrogenbenzyl T835 methyl hydrogen 2-fluorophenyl T836 methyl hydrogen3-fluorophenyl T837 methyl hydrogen 4-fluorophenyl T838 methyl hydrogen2-chlorophenyl T839 methyl hydrogen 3-chlorophenyl T840 methyl hydrogen4-chlorophenyl T841 ethyl hydrogen phenyl T842 ethyl hydrogen benzylT843 ethyl hydrogen 2-fluorophenyl T844 ethyl hydrogen 3-fluorophenylT845 ethyl hydrogen 4-fluorophenyl T846 ethyl hydrogen 2-chlorophenylT847 ethyl hydrogen 3-chlorophenyl T848 ethyl hydrogen 4-chlorophenylT849 thien-2-yl hydrogen phenyl T850 thien-2-yl hydrogen benzyl T851thien-2-yl hydrogen 2-fluorophenyl T852 thien-2-yl hydrogen3-fluorophenyl T853 thien-2-yl hydrogen 4-fluorophenyl T854 thien-2-ylhydrogen 2-chlorophenyl T855 thien-2-yl hydrogen 3-chlorophenyl T856thiene-2-yl hydrogen 4-chlorophenyl

Compounds according to the second aspect of Category X can be preparedby the procedure outlined in Scheme XXIII and described herein below inExample 24.

Example 24{4-[2-(S)-(4-Ethyloxazol-2-yl)-2-phenylacetylaminoethyl]-phenyl}sulfamicacid (67)

Preparation of (S)-1-(4-ethyloxazol-2-yl)-2-(4-nitrophenyl)ethanamine(65): A mixture of [1-(S)-carbamoyl-2-(4-nitrophenyl)ethyl-carbamic acidtert-butyl ester, 1, (10 g, 32.3 mmol) and 1-bromo-2-butanone (90%, 4.1mL, 36 mmol) in CH₃CN (500 mL) is refluxed for 18 hours. The reactionmixture is cooled to room temperature and diethyl ether is added to thesolution and the precipitate which forms is removed by filtration and isused without further purification.

Preparation ofN-[1-(4-ethyloxazol-2-yl)-2-(4-nitrophenyl)ethyl]-2-phenyl-acetamide(66): To a solution of(S)-1-(4-ethyloxazol-2-yl)-2-(4-nitrophenyl)ethanamine, 65, (2.9 g, 11mmol), phenylacetic acid (1.90 g, 14 mmol) and 1-hydroxybenzotriazole(HOBt) (0.94 g, 7.0 mmol) in DMF (100 mL) at 0° C., is added1-(3-dimethylamino-propyl)-3-ethylcarbodiimide (EDCI) (2.68 g, 14 mmol)followed by triethylamine (6.0 mL, 42 mmol). The mixture is stirred at0° C. for 30 minutes then at room temperature overnight. The reactionmixture is diluted with water and extracted with EtOAc. The combinedorganic phase is washed with 1 N aqueous HCl, 5% aqueous NaHCO₃, waterand brine, and dried over Na₂SO₄. The solvent is removed in vacuo toafford the desired product which is used without further purification.

Preparation of{4-[2-(S)-(4-ethyloxazol-2-yl)-2-phenylacetylaminoethyl]-phenyl}sulfamicacid (67):N-[1-(4-ethyloxazol-2-yl)-2-(4-nitrophenyl)ethyl]-2-phenyl-acetamide,66, (0.260 g) is dissolved in MeOH (4 mL). A catalytic amount of Pd/C(10% w/w) is added and the mixture is stirred under a hydrogenatmosphere 18 hours. The reaction mixture is filtered through a bed ofCELITE™ and the solvent is removed under reduced pressure. The crudeproduct is dissolved in pyridine (12 mL) and treated with SO₃-pyridine(0.177 g, 1.23). The reaction is stirred at room temperature for 5minutes after which a 7% solution of NH₄OH (10 mL) is added. The mixtureis then concentrated and the resulting residue is purified by reversephase chromatography to afford the desired product as the ammonium salt.

METHODS

The disclosed compounds can be used to prevent, abate, minimize,control, and/or lessen tumor metastasis in humans and animals. Thedisclosed compounds can also be used to slow the rate of primary tumorgrowth. The disclosed compounds when administered to a subject in needof treatment can be used to stop the spread of cancer cells. As such,the compounds disclosed herein can be administered as part of acombination therapy with one or more drugs or other pharmaceuticalagents. When used as part of the combination therapy, the decrease inmetastasis and reduction in primary tumor growth afforded by thedisclosed compounds allows for a more effective and efficient use of anypharmaceutical or drug therapy being used to treat the patient. Inaddition, control of metastasis by the disclosed compound affords thesubject a greater ability to concentrate the disease in one location.

Disclosed herein are methods for preventing metastasis of malignanttumors or other cancerous cells as well as to reduce the rate of tumorgrowth. The methods comprise administering an effective amount of one ormore of the disclosed compounds to a subject diagnosed with a malignanttumor or cancerous cells or to a subject having a tumor or cancerouscells.

Further disclosed herein is the use of the disclosed compounds formaking a medicament for preventing metastasis of malignant tumors orother cancerous cells and for slowing tumor growth.

The following are non-limiting examples of cancers that can be treatedby the disclosed methods and compositions: Acute Lymphoblastic; AcuteMyeloid Leukemia; Adrenocortical Carcinoma; Adrenocortical Carcinoma,Childhood; Appendix Cancer; Basal Cell Carcinoma; Bile Duct Cancer,Extrahepatic; Bladder Cancer; Bone Cancer; Osteosarcoma and MalignantFibrous Histiocytoma; Brain Stem Glioma, Childhood; Brain Tumor, Adult;Brain Tumor, Brain Stem Glioma, Childhood; Brain Tumor, Central NervousSystem Atypical Teratoid/Rhabdoid Tumor, Childhood; Central NervousSystem Embryonal Tumors; Cerebellar Astrocytoma; CerebralAstrocytotna/Malignant Glioma; Craniopharyngioma; Ependymoblastoma;Ependymoma; Medulloblastoma; Medulloepithelioma; Pineal ParenchymalTumors of intermediate Differentiation; Supratentorial PrimitiveNeuroectodermal Tumors and Pineoblastoma; Visual Pathway andHypothalamic Glioma; Brain and Spinal Cord Tumors; Breast Cancer;Bronchial Tumors; Burkitt Lymphoma; Carcinoid Tumor; Carcinoid Tumor,Gastrointestinal; Central Nervous System Atypical Teratoid/RhabdoidTumor; Central Nervous System Embryonal Tumors; Central Nervous SystemLymphoma; Cerebellar Astrocytoma Cerebral Astrocytoma/Malignant Glioma,Childhood; Cervical Cancer; Chordoma, Childhood; Chronic LymphocyticLeukemia; Chronic Myelogenous Leukemia; Chronic MyeloproliferativeDisorders; Colon Cancer; Colorectal Cancer; Craniopharyngioma; CutaneousT-Cell Lymphoma; Esophageal Cancer; Ewing Family of Tumors; ExtragonadalGerm Cell Tumor; Extrahepatic Bile Duct Cancer; Eye Cancer, intraocularMelanoma; Eye Cancer, Retinoblastoma; Gallbladder Cancer; Gastric(Stomach) Cancer; Gastrointestinal Carcinoid Tumor; GastrointestinalStromal Tumor (GIST); Germ Cell Tumor, Extracranial; Germ Cell Tumor,Extragonadal; Germ Cell Tumor, Ovarian; Gestational Trophoblastic Tumor;Glioma; Glioma, Childhood Brain Stem; Glioma, Childhood CerebralAstrocytoma; Glioma, Childhood Visual Pathway and Hypothalamic; HairyCell Leukemia; Head and Neck Cancer; Hepatocellular (Liver) Cancer;Histiocytosis, Langerhans Cell; Hodgkin Lymphoma;http://www.cancer.gov/cancertopics/types/hodgkinslymphoma/HypopharyngealCancer; Hypothalamic and Visual Pathway Glioma; intraocular Melanoma;Islet Cell Tumors; Kidney (Renal Cell) Cancer; Langerhans CellHistiocytosis; Laryngeal Cancer; Leukemia, Acute Lymphoblastic;Leukemia, Acute Myeloid; Leukemia, Chronic Lymphocytic; Leukemia,Chronic Myelogenous; Leukemia, Hairy Cell; Lip and Oral Cavity Cancer;Liver Cancer; Lung Cancer, Non-Small Cell; Lung Cancer, Small Cell;Lymphoma, AIDS-Related; Lymphoma, Burkitt; Lymphoma, Cutaneous T-Cell;Lymphoma, Hodgkin; Lymphoma, Non-Hodgkin; Lymphoma, Primary CentralNervous System; Macroglobulinemia, Waldenström; Malignant FibrousHistiocvtoma of Bone and Osteosarcoma; Medulloblastoma; Melanoma;Melanoma, intraocular (Eye); Merkel Cell Carcinoma; Mesothelioma;Metastatic Squamous Neck Cancer with Occult Primary; Mouth Cancer;Multiple Endocrine Neoplasia Syndrome, (Childhood); MultipleMyeloma/Plasma Cell Neoplasm; Mycosis; Fungoides; MyelodysplasticSyndromes; Myelodysplastic/Myeloproliferative Diseases; MyelogenousLeukemia, Chronic; Myeloid Leukemia, Adult Acute; Myeloid Leukemia,Childhood Acute; Myeloma, Multiple; Myeloproliferative Disorders,Chronic; Nasal Cavity and Paranasal Sinus Cancer; Nasopharyngeal Cancer;Neuroblastoma; Non-Small Cell Lung Cancer; Oral Cancer; Oral CavityCancer; Oropharyngeal Cancer; Osteosarcoma and Malignant FibrousHistiocytoma of Bone; Ovarian Cancer; Ovarian Epithelial Cancer; OvarianGerm Cell Tumor; Ovarian Low Malignant Potential Tumor; PancreaticCancer; Pancreatic Cancer, Islet Cell Tumors; Papillomatosis;Parathyroid Cancer; Penile Cancer; Pharyngeal Cancer; Pheochromocytoma;Pineal Parenchymal Tumors of Intermediate Differentiation; Pineoblastomaand Supratentorial Primitive Neuroectodermal Tumors; Pituitary Tumor;Plasma Celt Neoplasm/Multiple Myeloma; Pleuropulmonary Blastoma; PrimaryCentral Nervous System Lymphoma; Prostate Cancer; Rectal Cancer; RenalCell (Kidney) Cancer; Renal Pelvis and Ureter, Transitional Cell Cancer;Respiratory Tract Carcinoma Involving the NUT Gene on Chromosome 15;Retinoblastoma; Rhabdomyosarcoma; Salivary Gland Cancer; Sarcoma, EwingFamily of Tumors; Sarcoma, Kaposi; Sarcoma, Soft Tissue; Sarcoma,Uterine; Sézary Syndrome; Skin Cancer (Nonmelanoma); Skin Cancer(Melanoma); Skin Carcinoma, Merkel Cell; Small Cell Lung Cancer; SmallIntestine Cancer; Soft Tissue Sarcoma; Squamous Cell Carcinoma, SquamousNeck Cancer with Occult Primary, Metastatic; Stomach (Gastric) Cancer;Supratentorial Primitive Neuroectodermal Tumors; T-Cell Lymphoma,Cutaneous; Testicular Cancer; Throat Cancer; Thymoma and ThymicCarcinoma; Thyroid Cancer; Transitional Cell Cancer of the Renal Pelvisand Ureter; Trophoblastic Tumor, Gestational; Urethral Cancer; UterineCancer, Endometrial; Uterine Sarcoma; Vaginal Cancer; Vulvar Cancer;Waldenström Macroglobulinemia; and Wilms Tumor.

In Vivo Anti-Metastasis StudyA total of 50 NCr nu/nu male mice aged 5-6weeks were selected for the following in vivo test. H460-GFP human tumorcells were grown subcutaneously in NCr nu/nu male mice after which theresulting tumors were harvested. Subsequent to harvesting, recipient NCrnu/nu mice of this study had tumor fragments transplanted by surgicalorthotopic implantation (SOI). Each animal was anesthetized is isofuraneand the surgical area was sterilized with iodine and alcohol. Atransverse incision approximately 1.5 cm long was made in the left chestwall of the mice using a pair of surgical scissors. An intercostalincision was made between the third and the fourth costa and the leftlung was exposed. Two pieces of H460-GFP tumor fragments weretransplanted to the surface of the lung with 8-0 surgical nylon suture.The chest wall was closed with 6-0 silk suture. The lung was re-inflatedby the intrathoracic puncture using 3 cc syringe with 25 G 1½ needle todraw out the remaining air in the chest cavity. The chest wall wasclosed with 6-0 surgical silk sutures. All procedures of the operationdescribed above were performed with a 7× magnification microscope(Olympus) under HEPA filtered laminar flow hoods.

The treated mice were divided into five groups containing ten mice each.Group I (Control) received 100 μL the drug vehicle twice daily for 15days. Group II received 15 mg/kg of taxol intravenously at days 6, 9,12, and 15. Group III received 15 mg/kg of taxol intravenously at days6, 9, 12, and 15 and 15 mg/kg of4-{(S)-2-[(S)-2-(methoxycarbonylamino)-3-phenyl-propanamido]-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid, D91, (Test Compound) twice per day from day 3 to day 15. Group IVreceived 10 mg/kg of taxol intravenously at days 6, 9, 12, and 15 and 15mg/kg of the Test Compound twice per day from day 3 to day 15. Group Vreceived 15 mg/kg of the Test Compound twice per day from day 3 to day15. All animals were sacrificed on day 28.

Each animal was checked twice a week for primary tumor and metastasisvia GFP open imaging of the mediastinal lymph nodes. No metastasis wasfound on any other organs. Six mice from the control group died beforethe end of the study, but were evaluated for tumor and/or cellularmetastasis. Table XXI below summarizes the results of this in vivometastasis study.

TABLE XXI Mouse No. Group I Group II Group III Group IV Group V Evidenceof Metastasis 1 Yes No No No No 2 Yes No No Yes Yes 3 No Yes No No No 4Yes No No No No 5 Yes No No No No 6 Yes No No No Yes 7 Yes No No No No 8No No No No No 9 Yes No No Yes No 10  Yes No No No No # mice with 8 1 02 2 metastases

In Vivo Anticancer Studies B16 Melanoma

The effectiveness of the disclosed compounds as a treatment for melanomawas studied. C57BL/6 mice (6-8 weeks-old, female, Taconic Farm) wereinoculated with B16 melanoma cells (5×10⁴ cells/site, s.c.). On day 4post-inoculation, the mice (5/group) were treated (5 day/week, M-F) withvehicle control (bid, i.p.), low dose of IL-2 (100,000 IU/mouse, bid,i.p.), high dose of IL-2 (300,000 IU/mouse, bid, i.p.), or theircombinations with D91 (40 mg/kg, bid, s.c., administered 30 minutesprior to IL-2). Tumor volumes were recorded. In a separate experiment,mice similarly inoculated with B 16 melanoma cells were treated (5day/week, M-F) with vehicle control (bid, i.p.), IL-2 (300,000 IU/mouse,bid, i.p.), high dose of D91 (40 mg/kg, bid, s.c.), low dose of D91 (10mg/kg, bid, s.c.) or the combination of IL-2 (300,000 IU/mouse, bid,i.p.) with low dose of D91 (10 mg/kg, bid, s.c., administered 30 minutesprior to IL-2). Tumor volumes were recorded.

As depicted in FIG. 3, although the high dose of IL-2 (⋄) reduced tumorvolume as expected versus control (□), treatment with high dose of IL-2in combination with high dose of D91 (◯) significantly reduced tumorvolume as compared to high dose of IL-2 (⋄) alone. FIG. 4 is a histogramrepresenting the tumor volume of animals receiving control (A), highdose IL-2 (B), and high dose IL-2/high dose D91 (C). The data depictedin FIG. 5 indicate that the increase in animal body weights during thestudy were consistent over each group and did not show any effects dueto the choice of treatment.

As depicted in FIG. 6, treatment with a low dose of IL-2 in combinationwith a high dose of D91 (◯) significantly reduced tumor volume versuscontrol (□), whereas low dose of IL-2 (⋄) alone did not show measurabletumor volume reduction at the termination of the study on day 22. FIG. 7is a histogram representing the tumor volume of animals receivingcontrol (A), low dose IL-2 (B), and low dose IL-2/high dose D91 (C).FIG. 8 shows the effectiveness of treatments with 40 mg/kg b.i.d. D91(◯) versus vehicle control (□) on B16 melanoma tumor volume.

Renal Cell Carcinoma

The effectiveness of the disclosed compounds as a treatment againstrenal cell carcinoma was studied. Balb/c mice (6-8 weeks-old, female,Taconic Farm) were inoculated with Renca renal cancer cells (5×10⁵cells/site). On day 4 post-inoculation, the mice were randomly dividedinto 4 groups (4 mice/group) and treated (5 day/week, M-F) with vehiclecontrol (bid, i.p.), IL-2 (100,000 IU/mouse, bid, i.p.), D91 (40 mg/kg,bid, s.c., administered 30 minutes prior to IL-2) or the combination.Tumor volumes were recorded.

As depicted in FIG. 1 and FIG. 2,4-{(S)-2-[(S)-2-(methoxycarbonylamino)-3-phenyl-propanamido]-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid, D91, significantly redcued renal tumor growth and was as effectiveas IL-2 in reducing tumor volume. In addition, the combination of D91and IL-2 further reduced tumor volume.

Pancreatic Cancer

Pancreatic cancer is a malignant neoplasm of the pancreas. Each year inthe United States, about 42,000 individuals are diagnosed with thiscondition and 35,000 die from the disease (“Pancreatic Cancer—NationalCancer Institute, U.S. National Institutes of Health” (2009)). Theprognosis is relatively poor but has improved; the three-year survivalrate is now about thirty percent, but less than 5 percent of thosediagnosed are still alive five years after diagnosis. Complete remissionis still rather rare (Ghaneh P et al., (August 2007). “Biology andmanagement of pancreatic cancer”. Gut 56 (8): 1134-52).

The effectiveness of the disclosed compounds as a treatment forpancreatic cancer was investigated. 60 NCr nu/nu males, 5-6 weeks oldwere transplanted by surgical orthotopic implantation (SOI) usingMiaPaCa-2-RFP tumor fragments harvested from stock animals. The animalswere anesthetized with isoflurane and the surgical area was sterilizedusing iodine and alcohol. An incision approximately 1.5 cm long was madein the left upper abdomen of the nude mouse using a pair of surgicalscissors. The pancreas was exposed, and then two pieces of theMiaPaCa-2-RFP tumor fragments of 2 mm³ were transplanted to the mousepancreas with 8-0 surgical sutures (nylon) after the capsule of thetransplantation site had been stripped. The abdomen was closed with 6-0surgical sutures (silk). All procedures of the operation described abovewere performed with a 7× magnification microscope (Olympus) under HEPAfiltered laminar flow hoods.

Dosing of the animals began three days following tumor implantation. Thehigh dose of gemcitabine was 150 mg/kg. The low dose of gemcitabine was100 mg/kg. D91 was given at 20 mg/kg. Gemcitabine was give i.p. whereasD91 was given s.c.

The animals were divided into the following groups of ten animals eachas depicted in Table XXII. As indicated in Table XXII, D91 wasadministered twice daily whereas gemcitabine was given twice weekly.

TABLE XXII Group Agent Dose Schedule 1 vehicle 100 μL b.i.d 2gemcitabine 150 mg/kg Twice weekly 3 gemicitabine + D91 150 mg/kg + 20mg/kg Twice weekly + b.i.d. 4 gemcitabine 100 mg/kg Twice weekly 5gemicitabine + D91 100 mg/kg + 20 mg/kg Twice weekly + b.i.d. 6 D91  20mg/kg b.i.d

As depicted in FIG. 9, D91 given at 20 mg/kg showed a significantreduction in pancreatic tumor volume at day 28 (◯). In addition thecombination of low dose gemcitabine and 20 mg/kg D91 (*) showed agreater reduction in tumor volume than either low dose gencitabine (X)or D91 alone (◯).

Disclosed herein is a method for treating carcinoma in a subject,comprising administering to the subject having a carcinoma an effectiveamount of one or more of the disclosed compounds.

Also disclosed herein is a method for treating a subject diagnosed withcancer, comprising administering to the subject an effective amount ofone or more of the disclosed compounds.

Further disclosed herein is a method for treating carcinoma in asubject, comprising administering to the subject having a carcinoma acomposition comprising:

a) an effective amount of one or more of the disclosed compounds; and

b) an effective amount of an anticancer drug.

Still further disclosed herein is a method for treating carcinoma in asubject, comprising administering to the subject having a carcinoma acomposition comprising:

a) an effective amount of one or more of the disclosed compounds; and

b) an effective amount of a compound that inhibits tumor growth.

Yet further disclosed herein is a method for treating a subjectdiagnosed with cancer, comprising administering to the subject diagnosedwith cancer a composition comprising:

a) an effective amount of one or more of the disclosed compounds; and

b) an effective amount of an anticancer drug.

Still yet further disclosed herein is a method for treating a subjectdiagnosed with cancer, comprising administering to the subject diagnosedwith cancer a composition comprising:

a) an effective amount of one or more of the disclosed compounds; and

b) an effective amount of a compound that inhibits tumor growth.

Compositons

Disclosed herein are compositions which can be used to preventmetastasis of cancer cells in a subject, the compositions comprising aneffective amount of one or more of the compounds disclosed herein.Further disclosed herein are compositions that can be used to treattumors in a human or other mammal.

One aspect relates to a composition comprising:

a) an effective amount of one or more compounds disclosed herein; and

b) one or more pharmaceutically acceptable ingredients.

Another aspect relates a composition comprising:

a) an effective amount of one or more compounds disclosed herein; and

b) an effective amount of one or chemotherapeutic agents;

-   -   wherein the disclosed compounds and the chemotherapeutic agents        can be administered together or in any order.

One embodiment relates to a compostion comprising:

a) an effective amount of one or more compounds disclosed herein; and

b) an effective amount of taxol;

-   -   wherein the disclosed compounds and taxol can be administered        together or in any order.

Another embodiment relates to a composition comprising:

a) an effective amount of one or more compounds disclosed herein; and

b) an effective amount of gemcitabine;

-   -   wherein the disclosed compounds and gemcitabine can be        administered together or in any order.

A further embodiment relate to a composition comprising:

a) an effective amount of one or more compounds disclosed herein; and

b) an effective amount of erlotinib;

-   -   wherein the disclosed compounds and erlotinib can be        administered together or in any order.

A yet further embodiment relate to a composition comprising:

a) an effective amount of one or more compounds disclosed herein; and

b) an effective amount of doxil;

-   -   wherein the disclosed compounds and doxil can be administered        together or in any order.

A still further embodiment relate to a composition comprising:

a) an effective amount of one or more compounds disclosed herein; and

b) an effective amount of irinortecan;

-   -   wherein the disclosed compounds and irinortecan can be        administered together or in any order.

A still yet further embodiment relate to a composition comprising:

a) an effective amount of one or more compounds disclosed herein; and

b) an effective amount of bevacizumab;

-   -   wherein the disclosed compounds and bevacizumab can be        administered together or in any order.

A “chemotherapeutic agent” or “chemotherapeutic compound” is a chemicalcompound useful in the treatment of cancer. Chemotherapeutic canceragents that can be used in combination with those disclosed hereininclude, but are not limited to, mitotic inhibitors (vinca alkaloids).These include vincristine, vinblastine, vindesine and Navelbine™(vinorelbine-5′-noranhydroblastine). In yet other embodiments,chemotherapeutic cancer agents include topoisomerase I inhibitors, suchas camptothecin compounds. As used herein, “camptothecin compounds”include Camptosar™ (irinotecan HCL), Hycamtin™ (topotecan HCL) and othercompounds derived from camptothecin and its analogues. Another categoryof chemotherapeutic cancer agents that may be used in the methods andcompositions of the present disclosure are podophyllotoxin derivatives,such as etoposide, teniposide and mitopodozide. The present disclosurefurther encompasses other chemotherapeutic cancer agents known asalkylating agents, which alkylate the genetic material in tumor cells.These include without limitation cisplatin, cyclophosphamide, nitrogenmustard, trimethylene thiophosphoramide, carmustine, busulfan,chlorambucil, belustine, uracil mustard, chlomaphazin, and dacarbazine.The present disclosure encompasses antimetabolites as chemotherapeuticagents. Examples of these types of agents include cytosine arabinoside,fluorouracil, methotrexate, mercaptopurine, azathioprime, andprocarbazine. An additional category of chemotherapeutic cancer agentsthat may be used in the methods and compositions of the presentdisclosure include antibiotics. Examples include without limitationdoxorubicin, bleomycin, dactinomycin, daunorubicin, mithramycin,mitomycin, mytomycin C, and daunomycin. There are numerous liposomalformulations commercially available for these compounds. The presentdisclosure further encompasses other chemotherapeutic cancer agentsincluding without limitation anti-tumor antibodies, dacarbazine,azacytidine, amsacrine, melphalan, ifosfamide and mitoxantrone.

The disclosed compounds herein can be administered alone or incombination with other anti-tumor agents, includingcytotoxic/antineoplastic agents and anti-angiogenic agents.Cytotoxic/anti-neoplastic agents are defined as agents which attack andkill cancer cells. Some cytotoxic/anti-neoplastic agents are alkylatingagents, which alkylate the genetic material in tumor cells, e.g.,cis-platin, cyclophosphamide, nitrogen mustard, trimethylenethiophosphoramide, carmustine, busulfan, chlorambucil, belustine, uracilmustard, chlomaphazin, and dacabazine. Other cytotoxic/anti-neoplasticagents are antimetabolites for tumor cells, e.g., cytosine arabinoside,fluorouracil, methotrexate, mercaptopuirine, azathioprime, andprocarbazine. Other cytotoxic/anti-neoplastic agents are antibiotics,e.g., doxorubicin, bleomycin, dactinomycin, daunorubicin, mithramycin,mitomycin, mytomycin C, and daunomycin. There are numerous liposomalformulations commercially available for these compounds. Still othercytotoxic/anti-neoplastic agents are mitotic inhibitors (vincaalkaloids). These include vincristine, vinblastine and etoposide.Miscellaneous cytotoxic/anti-neoplastic agents include taxol and itsderivatives, L-asparaginase, anti-tumor antibodies, dacarbazine,azacytidine, amsacrine, melphalan, VM-26, ifosfamide, mitoxantrone, andvindesine.

Anti-angiogenic agents are well known to those of skill in the art.Suitable anti-angiogenic agents for use in the methods and compositionsof the present disclosure include anti-VEGF antibodies, includinghumanized and chimeric antibodies, anti-VEGF aptamers and antisenseoligonucleotides. Other known inhibitors of angiogenesis includeangiostatin, endostatin, interferons, interleukin 1 (including α and β)interleukin 12, retinoic acid, and tissue inhibitors ofmetalloproteinase-1 and -2. (TIMP-1 and -2). Small molecules, includingtopoisomerases such as razoxane, a topoisomerase II inhibitor withanti-angiogenic activity, can also be used.

Other anti-cancer agents that can be used in combination with thedisclosed compounds include, but are not limited to: acivicin;aclarubicin; acodazole hydrochloride; acronine; adozelesin; aldesleukin;altretamine; ambomycin; ametantrone acetate; aminoglutethimide;amsacrine; anastrozole; anthramycin; asparaginase; asperlin;azacitidine; azetepa; azotomycin; batimastat; benzodepa; bicalutamide;bisantrene hydrochloride; bisnafide dimesylate; bizelesin; bleomycinsulfate; brequinar sodium; bropirimine; busulfan; cactinomycin;calusterone; caracemide; carbetimer; carboplatin; carmustine; carubicinhydrochloride; carzelesin; cedefingol; chlorambucil; cirolemycin;cisplatin; cladribine; crisnatol mesylate; cyclophosphamide; cytarabine;dacarbazine; dactinomycin; daunorubicin hydrochloride; decitabine;dexormaplatin; dezaguanine; dezaguanine mesylate; diaziquone; docetaxel;doxorubicin; doxorubicin hydrochloride; droloxifene; droloxifenecitrate; dromostanolone propionate; duazomycin; edatrexate; eflornithinehydrochloride; elsamitrucin; enloplatin; enpromate; epipropidine;epirubicin hydrochloride; erbulozole; esorubicin hydrochloride;estramustine; estramustine phosphate sodium; etanidazole; etoposide;etoposide phosphate; etoprine; fadrozole hydrochloride; fazarabine;fenretinide; floxuridine; fludarabine phosphate; fluorouracil;fluorocitabine; fosquidone; fostriecin sodium; gemcitabine; gemcitabinehydrochloride; hydroxyurea; idarubicin hydrochloride; ifosfamide;ilmofosine; interleukin II (including recombinant interleukin II, orrIL2), interferon alfa-2a; interferon alfa-2b; interferon alfa-n1;interferon alfa-n3; interferon beta-I a; interferon gamma-I b;iproplatin; irinotecan hydrochloride; lanreotide acetate; letrozole;leuprolide acetate; liarozole hydrochloride; lometrexol sodium;lomustine; losoxantrone hydrochloride; masoprocol; maytansine;mechlorethamine hydrochloride; megestrol acetate; melengestrol acetate;melphalan; menogaril; mercaptopurine; methotrexate; methotrexate sodium;metoprine; meturedepa; mitindomide; mitocarcin; mitocromin; mitogillin;mitomalcin; mitomycin; mitosper; mitotane; mitoxantrone hydrochloride;mycophenolic acid; nocodazole; nogalamycin; ormaplatin; oxisuran;paclitaxel; pegaspargase; peliomycin; pentamustine; peplomycin sulfate;perfosfamide; pipobroman; piposulfan; piroxantrone hydrochloride;plicamycin; plomestane; porfimer sodium; porfiromycin; prednimustine;procarbazine hydrochloride; puromycin; puromycin hydrochloride;pyrazofurin; riboprine; rogletimide; safingol; safingol hydrochloride;semustine; simtrazene; sparfosate sodium; sparsomycin; spirogermaniumhydrochloride; spiromustine; spiroplatin; streptonigrin; streptozocin;sulofenur; talisomycin; tecogalan sodium; tegafur; teloxantronehydrochloride; temoporfin; teniposide; teroxirone; testolactone;thiamiprine; thioguanine; thiotepa; tiazofurin; tirapazamine; toremifenecitrate; trestolone acetate; triciribine phosphate; trimetrexate;trimetrexate glucuronate; triptorelin; tubulozole hydrochloride; uracilmustard; uredepa; vapreotide; verteporfin; vinblastine sulfate;vincristine sulfate; vindesine; vindesine sulfate; vinepidine sulfate;vinglycinate sulfate; vinleurosine sulfate; vinorelbine tartrate;vinrosidine sulfate; vinzolidine sulfate; vorozole; zeniplatin;zinostatin; zorubicin hydrochloride. Other anti-cancer drugs include,but are not limited to: 20-epi-1,25 dihydroxyvitamin D3;5-ethynyluracil; abiraterone; aclarubicin; acylfulvene; adecypenol;adozelesin; aldesleukin; ALL-TK antagonists; altretamine; ambamustine;amidox; amifostine; aminolevulinic acid; amrubicin; amsacrine;anagrelide; anastrozole; andrographolide; angiogenesis inhibitors;antagonist D; antagonist G; antarelix; anti-dorsalizing morphogeneticprotein-1; antiandrogen, prostatic carcinoma; antiestrogen;antineoplaston; antisense oligonucleotides; aphidicolin glycinate;apoptosis gene modulators; apoptosis regulators; apurinic acid;ara-CDP-DL-PTBA; arginine deaminase; asulacrine; atamestane;atrimustine; axinastatin 1; axinastatin 2; axinastatin 3; azasetron;azatoxin; azatyrosine; baccatin III derivatives; balanol; batimastat;BCR/ABL antagonists; benzochlorins; benzoylstaurosporine; beta lactamderivatives; beta-alethine; betaclamycin B; betulinic acid; bFGFinhibitor; bicalutamide; bisantrene; bisaziridinylspermine; bisnafide;bistratene A; bizelesin; breflate; bropirimine; budotitane; buthioninesulfoximine; calcipotriol; calphostin C; camptothecin derivatives;canarypox IL-2; capecitabine; carboxamide-amino-triazole;carboxyamidotriazole; CaRest M3; CARN 700; cartilage derived inhibitor;carzelesin; casein kinase inhibitors (ICOS); castanospermine; cecropinB; cetrorelix; chlorins; chloroquinoxaline sulfonamide; cicaprost;cis-porphyrin; cladribine; clomifene analogues; clotrimazole;collismycin A; collismycin B; combretastatin A4; combretastatinanalogue; conagenin; crambescidin 816; crisnatol; cryptophycin 8;cryptophycin A derivatives; curacin A; cyclopentanthraquinones;cycloplatam; cypemycin; cytarabine ocfosfate; cytolytic factor;cytostatin; dacliximab; decitabine; dehydrodidemnin B; deslorelin;dexamethasone; dexifosfamide; dexrazoxane; dexverapamil; diaziquone;didemnin B; didox; diethylnorspermine; dihydro-5-azacytidine;dihydrotaxol, 9-; dioxamycin; diphenyl spiromustine; docetaxel;docosanol; dolasetron; doxifluridine; droloxifene; dronabinol;duocarmycin SA; ebselen; ecomustine; edelfosine; edrecolomab;eflornithine; elemene; emitefur; epirubicin; epristeride; estramustineanalogue; estrogen agonists; estrogen antagonists; etanidazole;etoposide phosphate; exemestane; fadrozole; fazarabine; fenretinide;filgrastim; finasteride; flavopiridol; flezelastine; fluasterone;fludarabine; fluorodaunorunicin hydrochloride; forfenimex; formestane;fostriecin; fotemustine; gadolinium texaphyrin; gallium nitrate;galocitabine; ganirelix; gelatinase inhibitors; gemcitabine; glutathioneinhibitors; hepsulfam; heregulin; hexamethylene bisacetamide; hypericin;ibandronic acid; idarubicin; idoxifene; idramantone; ilmofosine;ilomastat; imidazoacridones; imiquimod; immunostimulant peptides;insulin-like growth factor-1 receptor inhibitor; interferon agonists;interferons; interleukins; iobenguane; iododoxorubicin; ipomeanol, 4-;iroplact; irsogladine; isobengazole; isohomohalicondrin B; itasetron;jasplakinolide; kahalalide F; lamellarin-N triacetate; lanreotide;leinamycin; lenograstim; lentinan sulfate; leptolstatin; letrozole;leukemia inhibiting factor; leukocyte alpha interferon;leuprolide+estrogen+progesterone; leuprorelin; levamisole; liarozole;linear polyamine analogue; lipophilic disaccharide peptide; lipophilicplatinum compounds; lissoclinamide 7; lobaplatin; lombricine;lometrexol; lonidamine; losoxantrone; lovastatin; loxoribine;lurtotecan; lutetium texaphyrin; lysofylline; lytic peptides;maitansine; mannostatin A; marimastat; masoprocol; maspin; matrilysininhibitors; matrix metalloproteinase inhibitors; menogaril; merbarone;meterelin; methioninase; metoclopramide; MIF inhibitor; mifepristone;miltefosine; mirimostim; mismatched double stranded RNA; mitoguazone;mitolactol; mitomycin analogues; mitonafide; mitotoxin fibroblast growthfactor-saporin; mitoxantrone; mofarotene; molgramostim; monoclonalantibody, human chorionic gonadotrophin; monophosphoryl lipidA+myobacterium cell wall sk; mopidamol; multiple drug resistance geneinhibitor; multiple tumor suppressor 1-based therapy; mustard anticanceragent; mycaperoxide B; mycobacterial cell wall extract; myriaporone;N-acetyldinaline; N-substituted benzamides; nafarelin; nagrestip;naloxone+pentazocine; napavin; naphterpin; nartograstim; nedaplatin;nemorubicin; neridronic acid; neutral endopeptidase; nilutamide;nisamycin; nitric oxide modulators; nitroxide antioxidant; nitrullyn;O6-benzylguanine; octreotide; okicenone; oligonucleotides; onapristone;ondansetron; ondansetron; oracin; oral cytokine inducer; ormaplatin;osaterone; oxaliplatin; oxaunomycin; paclitaxel; paclitaxel analogues;paclitaxel derivatives; palauamine; palmitoylrhizoxin; pamidronic acid;panaxytriol; panomifene; parabactin; pazelliptine; pegaspargase;peldesine; pentosan polysulfate sodium; pentostatin; pentrozole;perflubron; perfosfamide; perillyl alcohol; phenazinomycin;phenylacetate; phosphatase inhibitors; picibanil; pilocarpinehydrochloride; pirarubicin; piritrexim; placetin A; placetin B;plasminogen activator inhibitor; platinum complex; platinum compounds;platinum-triamine complex; porfimer sodium; porfiromycin; prednisone;propyl bis-acridone; prostaglandin J2; proteasome inhibitors; proteinA-based immune modulator; protein kinase C inhibitor; protein kinase Cinhibitors, microalgal; protein tyrosine phosphatase inhibitors; purinenucleoside phosphorylase inhibitors; purpurins; pyrazoloacridine;pyridoxylated hemoglobin polyoxyethylene conjugate; raf antagonists;raltitrexed; ramosetron; ras farnesyl protein transferase inhibitors;ras inhibitors; ras-GAP inhibitor; retelliptine demethylated; rhenium Re186 etidronate; rhizoxin; ribozymes; RII retinamide; rogletimide;rohitukine; romurtide; roquinimex; rubiginone B1; ruboxyl; safingol;saintopin; SarCNU; sarcophytol A; sargramostim; Sdi 1 mimetics;semustine; senescence derived inhibitor 1; sense oligonucleotides;signal transduction inhibitors; signal transduction modulators; singlechain antigen binding protein; sizofuran; sobuzoxane; sodiumborocaptate; sodium phenylacetate; solverol; somatomedin bindingprotein; sonermin; sparfosic acid; spicamycin D; spiromustine;splenopentin; spongistatin 1; squalamine; stem cell inhibitor; stem-celldivision inhibitors; stipiamide; stromelysin inhibitors; sulfinosine;superactive vasoactive intestinal peptide antagonist; suradista;suramin; swainsonine; synthetic glycosaminoglycans; tallimustine;tamoxifen methiodide; tauromustine; tazarotene; tecogalan sodium;tegafur; tellurapyrylium; telomerase inhibitors; temoporfin;temozolomide; teniposide; tetrachlorodecaoxide; tetrazomine;thaliblastine; thiocoraline; thrombopoietin; thrombopoietin mimetic;thymalfasin; thymopoietin receptor agonist; thymotrinan; thyroidstimulating hormone; tin ethyl etiopurpurin; tirapazamine; titanocenebichloride; topsentin; toremifene; totipotent stem cell factor;translation inhibitors; tretinoin; triacetyluridine; triciribine;trimetrexate; triptorelin; tropisetron; turosteride; tyrosine kinaseinhibitors; tyrphostins; UBC inhibitors; ubenimex; urogenitalsinus-derived growth inhibitory factor; urokinase receptor antagonists;vapreotide; variolin B; vector system, erythrocyte gene therapy;velaresol; veramine; verdins; verteporfin; vinorelbine; vinxaltine;vitaxin; vorozole; zanoterone; zeniplatin; zilascorb; and zinostatinstimalamer. In one embodiment, the anti-cancer drug is 5-fluorouracil,taxol, or leucovorin.

The term “effective amount” as used herein means “an amount of one ormore phenylsulfamic acids, effective at dosages and for periods of timenecessary to achieve the desired or therapeutic result.” An effectiveamount may vary according to factors known in the art, such as thedisease state, age, sex, and weight of the human or animal beingtreated. Although particular dosage regimes may be described in examplesherein, a person skilled in the art would appreciated that the dosageregime may be altered to provide optimum therapeutic response. Thus, itis not possible to specify an exact “effective amount.” For example,several divided doses may be administered daily or the dose may beproportionally reduced as indicated by the exigencies of the therapeuticsituation. In addition, the compositions of the present disclosure canbe administered as frequently as necessary to achieve a therapeuticamount.

While particular embodiments of the present disclosure have beenillustrated and described, it would be obvious to those skilled in theart that various other changes and modifications can be made withoutdeparting from the spirit and scope of the disclosure. It is thereforeintended to cover in the appended claims all such changes andmodifications that are within the scope of this disclosure.

1. A method for preventing metastasis in a subject, comprisingcontacting the subject with an effective amount of a compound having theformula:

wherein R is a substituted or unsubstituted thiazolyl unit having theformula:

R², R³, and R⁴ are each independently: i) hydrogen; ii) substituted orunsubstituted C₁-C₆ linear, branched, or cyclic alkyl; iii) substitutedor unsubstituted C₂-C₆ linear, branched, or cyclic alkenyl; iv)substituted or unsubstituted C₂-C₆ linear or branched alkynyl; v)substituted or unsubstituted C₆ or C₁₀ aryl; vi) substituted orunsubstituted C₁-C₉ heteroaryl; vii) substituted or unsubstituted C₁-C₉heterocyclic; or viii) R² and R³ can be taken together to form asaturated or unsaturated ring having from 5 to 7 atoms; wherein from 1to 3 atoms can optionally be heteroatoms chosen from oxygen, nitrogen,and sulfur; Z is a unit having the formula:-(L)_(n)-R¹ R¹ is chosen from: i) hydrogen; ii) hydroxyl; iii) amino;iv) substituted or unsubstituted C₁-C₆ linear, branched or cyclic alkyl;v) substituted or unsubstituted C₁-C₆ linear, branched or cyclic alkoxy;vi) substituted or unsubstituted C₆ or C₁₀ aryl; vii) substituted orunsubstituted C₁-C₉ heterocyclic rings; or viii) substituted orunsubstituted C₁-C₉ heteroaryl rings; L is a linking unit having theformula:-[Q]_(y)[C(R^(5a)R^(5b))]_(x)[Q¹][C(R^(6a)R^(6b))]_(w)- Q and Q¹ areeach independently: i) —C(O)—; ii) —NH—; iii) —C(O)NH—; iv) —NHC(O)—; v)—NHC(O)NH—; vi) —NHC(O)O—; vii) —C(O)O—; viii) —C(O)NHC(O)—; ix) —O—; x)—S—; xi) —SO₂—; xii) —C(═NH)—; xiii) —C(═NH)NH—; xiv) —NHC(═NH)—; or xv)—NHC(═NH)NH—; R^(5a) and R^(5b) are each independently: i) hydrogen; ii)hydroxy; iii) halogen; iv) C₁-C₆ substituted or unsubstituted linear orbranched alkyl; or v) a unit having the formula:—[C(R^(7a)R^(7b))]_(t)R⁸ R^(7a) and R^(7b) are each independently: i)hydrogen; or ii) substituted or unsubstituted C₁-C₆ linear, branched, orcyclic alkyl; R⁸ is: i) hydrogen; ii) substituted or unsubstituted C₁-C₆linear, branched, or cyclic alkyl; iii) substituted or unsubstituted C₆or C₁₀ aryl; iv) substituted or unsubstituted C₁-C₉ heteroaryl; or v)substituted or unsubstituted C₁-C₉ heterocyclic; R^(6a) and R^(6b) areeach independently: i) hydrogen; or ii) C₁-C₄ linear or branched alkyl;the index n is 0 or 1; the indices t, w and x are each independentlyfrom 0 to 4; the indices y and z are each independently 0 or 1; or apharmaceutically acceptable salt thereof.
 2. A method for treating asubject with cancer, comprising administering to the subject andeffective amount of a compound having the formula:

wherein R is a substituted or unsubstituted thiazolyl unit having theformula:

R², R³, and R⁴ are each independently: i) hydrogen; ii) substituted orunsubstituted C₁-C₆ linear, branched, or cyclic alkyl; iii) substitutedor unsubstituted C₂-C₆ linear, branched, or cyclic alkenyl; iv)substituted or unsubstituted C₂-C₆ linear or branched alkynyl; v)substituted or unsubstituted C₆ or C₁₀ aryl; vi) substituted orunsubstituted C₁-C₉ heteroaryl; vii) substituted or unsubstituted C₁-C₉heterocyclic; or viii) R² and R³ can be taken together to form asaturated or unsaturated ring having from 5 to 7 atoms; wherein from 1to 3 atoms can optionally be heteroatoms chosen from oxygen, nitrogen,and sulfur; Z is a unit having the formula:-(L)_(n)-R¹ R¹ is chosen from: i) hydrogen; ii) hydroxyl; iii) amino;iv) substituted or unsubstituted C₁-C₆ linear, branched or cyclic alkyl;v) substituted or unsubstituted C₁-C₆ linear, branched or cyclic alkoxy;vi) substituted or unsubstituted C₆ or C₁₀ aryl; vii) substituted orunsubstituted C₁-C₉ heterocyclic rings; or viii) substituted orunsubstituted C₁-C₉ heteroaryl rings; L is a linking unit having theformula:-[Q]_(y)[C(R^(5a)R^(5b))]_(x)[Q¹][C(R^(6a)R^(6b))]_(w)- Q and Q¹ areeach independently: i) —C(O)—; ii) —NH—; iii) —C(O)NH—; iv) —NHC(O)—; v)—NHC(O)NH—; vi) —NHC(O)O—; vii) —C(O)O—; viii) —C(O)NHC(O)—; ix) —O—; x)—S—; xi) —SO₂—; xii) —C(═NH)—; xiii) —C(═NH)NH—; xiv) —NHC(═NH)—; or xv)—NHC(═NH)NH—; R^(5a) and R^(5b) are each independently: i) hydrogen; ii)hydroxy; iii) halogen; iv) C₁-C₆ substituted or unsubstituted linear orbranched alkyl; or v) a unit having the formula:—[C(R^(7a)R^(7b))]_(t)R⁸ R^(7a) and R^(7b) are each independently: i)hydrogen; or ii) substituted or unsubstituted C₁-C₆ linear, branched, orcyclic alkyl; R⁸ is: i) hydrogen; ii) substituted or unsubstituted C₁-C₆linear, branched, or cyclic alkyl; iii) substituted or unsubstituted C₆or C₁₀ aryl; iv) substituted or unsubstituted C₁-C₉ heteroaryl; or v)substituted or unsubstituted C₁-C₉ heterocyclic; R^(6a) and R^(6b) areeach independently: i) hydrogen; or ii) C₁-C₄ linear or branched alkyl;the index n is 0 or 1; the indices t, w and x are each independentlyfrom 0 to 4; the indices y and z are each independently 0 or 1; or apharmaceutically acceptable salt thereof.
 3. A method for treating asubject diagnosed with cancer, comprising administering to the subjectan effective amount of a compound having the formula:

wherein R is a substituted or unsubstituted thiazolyl unit having theformula:

R², R³, and R⁴ are each independently: i) hydrogen; ii) substituted orunsubstituted C₁-C₆ linear, branched, or cyclic alkyl; iii) substitutedor unsubstituted C₂-C₆ linear, branched, or cyclic alkenyl; iv)substituted or unsubstituted C₂-C₆ linear or branched alkynyl; v)substituted or unsubstituted C₆ or C₁₀ aryl; vi) substituted orunsubstituted C₁-C₉ heteroaryl; vii) substituted or unsubstituted C₁-C₉heterocyclic; or viii) R² and R³ can be taken together to form asaturated or unsaturated ring having from 5 to 7 atoms; wherein from 1to 3 atoms can optionally be heteroatoms chosen from oxygen, nitrogen,and sulfur; Z is a unit having the formula:-(L)_(n)-R¹ R¹ is chosen from: i) hydrogen; ii) hydroxyl; iii) amino;iv) substituted or unsubstituted C₁-C₆ linear, branched or cyclic alkyl;v) substituted or unsubstituted C₁-C₆ linear, branched or cyclic alkoxy;vi) substituted or unsubstituted C₆ or C₁₀ aryl; vii) substituted orunsubstituted C₁-C₉ heterocyclic rings; or viii) substituted orunsubstituted C₁-C₉ heteroaryl rings; L is a linking unit having theformula:-[Q]_(y)[C(R^(5a)R^(5b))]_(x)[Q¹]_(z)[C(R^(6a)R^(6b))]_(w)- Q and Q¹ areeach independently: i) —C(O)—; ii) —NH—; iii) —C(O)NH—; iv) —NHC(O)—; v)—NHC(O)NH—; vi) —NHC(O)O—; vii) —C(O)O—; viii) —C(O)NHC(O)—; ix) —O—; x)—S—; xi) —SO₂—; xii) —C(═NH)—; xiii) —C(═NH)NH—; xiv) —NHC(═NH)—; or xv)—NHC(═NH)NH—; R^(5a) and R^(5b) are each independently: i) hydrogen; ii)hydroxy; iii) halogen; iv) C₁-C₆ substituted or unsubstituted linear orbranched alkyl; or v) a unit having the formula:—[C(R^(7a)R^(7b))]_(t)R⁸ R^(7a) and R^(7b) are each independently: i)hydrogen; or ii) substituted or unsubstituted C₁-C₆ linear, branched, orcyclic alkyl; R⁸ is: i) hydrogen; ii) substituted or unsubstituted C₁-C₆linear, branched, or cyclic alkyl; iii) substituted or unsubstituted C₆or C₁₀ aryl; iv) substituted or unsubstituted C₁-C₉ heteroaryl; or v)substituted or unsubstituted C₁-C₉ heterocyclic; R^(6a) and R^(6b) areeach independently: i) hydrogen; or ii) C₁-C₄ linear or branched alkyl;the index n is 0 or 1; the indices t, w and x are each independentlyfrom 0 to 4; the indices y and z are each independently 0 or 1; or apharmaceutically acceptable salt thereof.
 4. A method for preventingmetastasis in a subject, comprising contacting the subject with acomposition comprising: A) an effective amount of one or more compoundshaving the formula:

wherein R is a substituted or unsubstituted thiazolyl unit having theformula:

R², R³, and R⁴ are each independently: i) hydrogen; ii) substituted orunsubstituted C₁-C₆ linear, branched, or cyclic alkyl; iii) substitutedor unsubstituted C₂-C₆ linear, branched, or cyclic alkenyl; iv)substituted or unsubstituted C₂-C₆ linear or branched alkynyl; v)substituted or unsubstituted C₆ or C₁₀ aryl; vi) substituted orunsubstituted C₁-C₉ heteroaryl; vii) substituted or unsubstituted C₁-C₉heterocyclic; or viii) R² and R³ can be taken together to form asaturated or unsaturated ring having from 5 to 7 atoms; wherein from 1to 3 atoms can optionally be heteroatoms chosen from oxygen, nitrogen,and sulfur; Z is a unit having the formula:-(L)_(n)-R¹ R¹ is chosen from: i) hydrogen; ii) hydroxyl; iii) amino;iv) substituted or unsubstituted C₁-C₆ linear, branched or cyclic alkyl;v) substituted or unsubstituted C₁-C₆ linear, branched or cyclic alkoxy;vi) substituted or unsubstituted C₆ or C₁₀ aryl; vii) substituted orunsubstituted C₁-C₉ heterocyclic rings; or viii) substituted orunsubstituted C₁-C₉ heteroaryl rings; L is a linking unit having theformula:-[Q]_(y)[C(R^(5a)R^(5b))]_(x)[Q¹][C(R^(6a)R^(6b))]_(w)- Q and Q¹ areeach independently: i) —C(O)—; ii) —NH—; iii) —C(O)NH—; iv) —NHC(O)—; v)—NHC(O)NH—; vi) —NHC(O)O—; vii) —C(O)O—; viii) —C(O)NHC(O)—; ix) —O—; x)—S—; xi) —SO₂—; xii) —C(═NH)—; xiii) —C(═NH)NH—; xiv) —NHC(═NH)—; or xv)—NHC(═NH)NH—; R^(5a) and R^(5b) are each independently: i) hydrogen; ii)hydroxy; iii) halogen; iv) C₁-C₆ substituted or unsubstituted linear orbranched alkyl; or v) a unit having the formula:—[C(R^(7a)R^(7b))]_(t)R⁸ R^(7a) and R^(7b) are each independently: i)hydrogen; or ii) substituted or unsubstituted C₁-C₆ linear, branched, orcyclic alkyl; R⁸ is: i) hydrogen; ii) substituted or unsubstituted C₁-C₆linear, branched, or cyclic alkyl; iii) substituted or unsubstituted C₆or C₁₀ aryl; iv) substituted or unsubstituted C₁-C₉ heteroaryl; or v)substituted or unsubstituted C₁-C₉ heterocyclic; R^(6a) and R^(6b) areeach independently: i) hydrogen; or ii) C₁-C₄ linear or branched alkyl;the index n is 0 or 1; the indices t, w and x are each independentlyfrom 0 to 4; the indices y and z are each independently 0 or 1; or apharmaceutically acceptable salt thereof; and B) an effective amount ofone or more chemotherapeutic agents.
 5. A method according to claim 4,wherein the chemotherapeutic agent is chosen from vincristine,vinblastine, vindesine, vinorelbine-5′-noranhydroblastine, irinotecan,topotecan, cisplatin, cyclophosphamide, nitrogen mustard, trimethylenethiophosphoramide, carmustine, busulfan, chlorambucil, belustine, uracilmustard, chlomaphazin, dacarbazine, cytosine arabinoside, fluorouracil,methotrexate, mercaptopurine, azathioprime, procarbazine, doxorubicin,bleomycin, dactinomycin, daunorubicin, mithramycin, mitomycin, mytomycinC, daunomycin, dacarbazine, azacytidine, amsacrine, melphalan,ifosfamide, mitoxantrone, cis-platin, cyclophosphamide, nitrogenmustard, trimethylene thiophosphoramide, carmustine, busulfan,chlorambucil, belustine, uracil mustard, chlomaphazin, dacabazine,cytosine arabinoside, fluorouracil, methotrexate, mercaptopuirine,azathioprime, procarbazine, doxorubicin, bleomycin, dactinomycin,daunorubicin, mithramycin, mitomycin, mytomycin C, daunomycin,vincristine, vinblastine, etoposide, taxol and its derivatives,L-asparaginase, anti-tumor antibodies, dacarbazine, azacytidine,amsacrine, melphalan, VM-26, ifosfamide, mitoxantrone, vindesine,acivicin; aclarubicin; acodazole hydrochloride; acronine; adozelesin;aldesleukin; altretamine; ambomycin; ametantrone acetate;aminoglutethimide; amsacrine; anastrozole; anthramycin; asparaginase;asperlin; azacitidine; azetepa; azotomycin; batimastat; benzodepa;bicalutamide; bisantrene hydrochloride; bisnafide dimesylate; bizelesin;bleomycin sulfate; brequinar sodium; bropirimine; busulfan;cactinomycin; calusterone; caracemide; carbetimer; carboplatin;carmustine; carubicin hydrochloride; carzelesin; cedefingol;chlorambucil; cirolemycin; cisplatin; cladribine; crisnatol mesylate;cyclophosphamide; cytarabine; dacarbazine; dactinomycin; daunorubicinhydrochloride; decitabine; dexormaplatin; dezaguanine; dezaguaninemesylate; diaziquone; docetaxel; doxorubicin; doxorubicin hydrochloride;droloxifene; droloxifene citrate; dromostanolone propionate; duazomycin;edatrexate; eflornithine hydrochloride; elsamitrucin; enloplatin;enpromate; epipropidine; epirubicin hydrochloride; erbulozole;esorubicin hydrochloride; estramustine; estramustine phosphate sodium;etanidazole; etoposide; etoposide phosphate; etoprine; fadrozolehydrochloride; fazarabine; fenretinide; floxuridine; fludarabinephosphate; fluorouracil; fluorocitabine; fosquidone; fostriecin sodium;gemcitabine; gemcitabine hydrochloride; hydroxyurea; idarubicinhydrochloride; ifosfamide; ilmofosine; interleukin II (includingrecombinant interleukin II, or rIL2), interferon alfa-2a; interferonalfa-2b; interferon alfa-n1; interferon alfa-n3; interferon beta-I a;interferon gamma-I b; iproplatin; irinotecan hydrochloride; lanreotideacetate; letrozole; leuprolide acetate; liarozole hydrochloride;lometrexol sodium; lomustine; losoxantrone hydrochloride; masoprocol;maytansine; mechlorethamine hydrochloride; megestrol acetate;melengestrol acetate; melphalan; menogaril; mercaptopurine;methotrexate; methotrexate sodium; metoprine; meturedepa; mitindomide;mitocarcin; mitocromin; mitogillin; mitomalcin; mitomycin; mitosper;mitotane; mitoxantrone hydrochloride; mycophenolic acid; nocodazole;nogalamycin; ormaplatin; oxisuran; paclitaxel; pegaspargase; peliomycin;pentamustine; peplomycin sulfate; perfosfamide; pipobroman; piposulfan;piroxantrone hydrochloride; plicamycin; plomestane; porfimer sodium;porfiromycin; prednimustine; procarbazine hydrochloride; puromycin;puromycin hydrochloride; pyrazofurin; riboprine; rogletimide; safingol;safingol hydrochloride; semustine; simtrazene; sparfosate sodium;sparsomycin; spirogermanium hydrochloride; spiromustine; spiroplatin;streptonigrin; streptozocin; sulofenur; talisomycin; tecogalan sodium;tegafur; teloxantrone hydrochloride; temoporfin; teniposide; teroxirone;testolactone; thiamiprine; thioguanine; thiotepa; tiazofurin;tirapazamine; toremifene citrate; trestolone acetate; triciribinephosphate; trimetrexate; trimetrexate glucuronate; triptorelin;tubulozole hydrochloride; uracil mustard; uredepa; vapreotide;verteporfin; vinblastine sulfate; vincristine sulfate; vindesine;vindesine sulfate; vinepidine sulfate; vinglycinate sulfate;vinleurosine sulfate; vinorelbine tartrate; vinrosidine sulfate;vinzolidine sulfate; vorozole; zeniplatin; zinostatin; zorubicinhydrochloride, 20-epi-1,25 dihydroxyvitamin D3; 5-ethynyluracil;abiraterone; aclarubicin; acylfulvene; adecypenol; adozelesin;aldesleukin; ALL-TK antagonists; altretamine; ambamustine; amidox;amifostine; amino]evulinic acid; amrubicin; amsacrine; anagrelide;anastrozole; andrographolide; angiogenesis inhibitors; antagonist D;antagonist G; antarelix; anti-dorsalizing morphogenetic protein-1;antiandrogen, prostatic carcinoma; antiestrogen; antineoplaston;antisense oligonucleotides; aphidicolin glycinate; apoptosis genemodulators; apoptosis regulators; apurinic acid; ara-CDP-DL-PTBA;arginine deaminase; asulacrine; atamestane; atrimustine; axinastatin 1;axinastatin 2; axinastatin 3; azasetron; azatoxin; azatyrosine; baccatinIII derivatives; balanol; batimastat; BCR/ABL antagonists;benzochlorins; benzoylstaurosporine; beta lactam derivatives;beta-alethine; betaclamycin B; betulinic acid; bFGF inhibitor;bicalutamide; bisantrene; bisaziridinylspermine; bisnafide; bistrateneA; bizelesin; breflate; bropirimine; budotitane; buthionine sulfoximine;calcipotriol; calphostin C; camptothecin derivatives; canarypox IL-2;capecitabine; carboxamide-amino-triazole; carboxyamidotriazole; CaRestM3; CARN 700; cartilage derived inhibitor; carzelesin; casein kinaseinhibitors (ICOS); castanospermine; cecropin B; cetrorelix; chlorins;chloroquinoxaline sulfonamide; cicaprost; cis-porphyrin; cladribine;clomifene analogues; clotrimazole; collismycin A; collismycin B;combretastatin A4; combretastatin analogue; conagenin; crambescidin 816;crisnatol; cryptophycin 8; cryptophycin A derivatives; curacin A;cyclopentanthraquinones; cycloplatam; cypemycin; cytarabine ocfosfate;cytolytic factor; cytostatin; dacliximab; decitabine; dehydrodidemnin B;deslorelin; dexamethasone; dexifosfamide; dexrazoxane; dexverapamil;diaziquone; didemnin B; didox; diethylnorspermine;dihydro-5-azacytidine; dihydrotaxol, 9-; dioxamycin; diphenylspiromustine; docetaxel; docosanol; dolasetron; doxifluridine;droloxifene; dronabinol; duocarmycin SA; ebselen; ecomustine;edelfosine; edrecolomab; eflornithine; elemene; emitefur; epirubicin;epristeride; estramustine analogue; estrogen agonists; estrogenantagonists; etanidazole; etoposide phosphate; exemestane; fadrozole;fazarabine; fenretinide; filgrastim; finasteride; flavopiridol;flezelastine; fluasterone; fludarabine; fluorodaunorunicinhydrochloride; forfenimex; formestane; fostriecin; fotemustine;gadolinium texaphyrin; gallium nitrate; galocitabine; ganirelix;gelatinase inhibitors; gemcitabine; glutathione inhibitors; hepsulfam;heregulin; hexamethylene bisacetamide; hypericin; ibandronic acid;idarubicin; idoxifene; idramantone; ilmofosine; ilomastat;imidazoacridones; imiquimod; immunostimulant peptides; insulin-likegrowth factor-1 receptor inhibitor; interferon agonists; interferons;interleukins; iobenguane; iododoxorubicin; ipomeanol, 4-; iroplact;irsogladine; isobengazole; isohomohalicondrin B; itasetron;jasplakinolide; kahalalide F; lamellarin-N triacetate; lanreotide;leinamycin; lenograstim; lentinan sulfate; leptolstatin; letrozole;leukemia inhibiting factor; leukocyte alpha interferon;leuprolide+estrogen+progesterone; leuprorelin; levamisole; liarozole;linear polyamine analogue; lipophilic disaccharide peptide; lipophilicplatinum compounds; lissoclinamide 7; lobaplatin; lombricine;lometrexol; lonidamine; losoxantrone; lovastatin; loxoribine;lurtotecan; lutetium texaphyrin; lysofylline; lytic peptides;maitansine; mannostatin A; marimastat; masoprocol; maspin; matrilysininhibitors; matrix metalloproteinase inhibitors; menogaril; merbarone;meterelin; methioninase; metoclopramide, and mitoguazone; mitolactol;mitomycin analogues; mitonafide; mitotoxin fibroblast growthfactor-saporin; mitoxantrone; mofarotene; molgramostim; monoclonalantibody, human chorionic gonadotrophin; monophosphoryl lipidA+myobacterium cell wall sk; mopidamol; multiple drug resistance geneinhibitor; multiple tumor suppressor 1-based therapy; mustard anticanceragent; mycaperoxide B; mycobacterial cell wall extract; myriaporone;N-acetyldinaline; N-substituted benzamides; nafarelin; nagrestip;naloxone+pentazocine; napavin; naphterpin; nartograstim; nedaplatin;nemorubicin; neridronic acid; neutral endopeptidase; nilutamide;nisamycin; nitric oxide modulators; nitroxide antioxidant; nitrullyn;O6-benzylguanine; octreotide; okicenone; oligonucleotides; onapristone;ondansetron; ondansetron; oracin; oral cytokine inducer; ormaplatin;osaterone; oxaliplatin; oxaunomycin; paclitaxel; paclitaxel analogues;paclitaxel derivatives; palauamine; palmitoylrhizoxin; pamidronic acid;panaxytriol; panomifene; parabactin; pazelliptine; pegaspargase;peldesine; pentosan polysulfate sodium; pentostatin; pentrozole;perflubron; perfosfamide; perillyl alcohol; phenazinomycin;phenylacetate; phosphatase inhibitors; picibanil; pilocarpinehydrochloride; pirarubicin; piritrexim; placetin A; placetin B;plasminogen activator inhibitor; platinum complex; platinum compounds;platinum-triamine complex; porfimer sodium; porfiromycin; prednisone;propyl bis-acridone; prostaglandin J2; proteasome inhibitors; proteinA-based immune modulator; protein kinase C inhibitor; protein kinase Cinhibitors, microalgal; protein tyrosine phosphatase inhibitors; purinenucleoside phosphorylase inhibitors; purpurins; pyrazoloacridine;pyridoxylated hemoglobin polyoxyethylene conjugate; raf antagonists;raltitrexed; ramosetron; ras farnesyl protein transferase inhibitors;ras inhibitors; ras-GAP inhibitor; retelliptine demethylated; rhenium Re186 etidronate; rhizoxin; ribozymes; RII retinamide; rogletimide;rohitukine; romurtide; roquinimex; rubiginone B1; ruboxyl; safingol;saintopin; SarCNU; sarcophytol A; sargramostim; Sdi 1 mimetics;semustine; senescence derived inhibitor 1; sense oligonucleotides;signal transduction inhibitors; signal transduction modulators; singlechain antigen binding protein; sizofuran; sobuzoxane; sodiumborocaptate; sodium phenylacetate; solverol; somatomedin bindingprotein; sonermin; sparfosic acid; spicamycin D; spiromustine;splenopentin; spongistatin 1; squalamine; stem cell inhibitor; stem-celldivision inhibitors; stipiamide; stromelysin inhibitors; sulfinosine;superactive vasoactive intestinal peptide antagonist; suradista;suramin; swainsonine; synthetic glycosaminoglycans; tallimustine;tamoxifen methiodide; tauromustine; tazarotene; tecogalan sodium;tegafur; tellurapyrylium; telomerase inhibitors; temoporfin;temozolomide; teniposide; tetrachlorodecaoxide; tetrazomine;thaliblastine; thiocoraline; thrombopoietin; thrombopoietin mimetic;thymalfasin; thymopoietin receptor agonist; thymotrinan; thyroidstimulating hormone; tin ethyl etiopurpurin; tirapazamine; titanocenebichloride; topsentin; toremifene; totipotent stem cell factor;translation inhibitors; tretinoin; triacetyluridine; triciribine;trimetrexate; triptorelin; tropisetron; turosteride; tyrosine kinaseinhibitors; tyrphostins; UBC inhibitors; ubenimex; urogenitalsinus-derived growth inhibitory factor; urokinase receptor antagonists;vapreotide; variolin B; vector system, erythrocyte gene therapy;velaresol; veramine; verdins; verteporfin; vinorelbine; vinxaltine;vitaxin; vorozole; zanoterone; zeniplatin; zilascorb; and zinostatinstimalamer.
 6. A method according to claim 4, wherein thechemotherapeutic agent is chosen from taxol, IL-2, gemcitabine,erlotinib, doxil, irinortecan, and bevacizumab.
 7. A method according toclaim 2, wherein the cancer is chosen from Acute Lymphoblastic; AcuteMyeloid Leukemia; Adrenocortical Carcinoma; Adrenocortical Carcinoma,Childhood; Appendix Cancer; Basal Cell Carcinoma; Bile Duct Cancer,Extrahepatic; Bladder Cancer; Bone Cancer; Osteosarcoma and MalignantFibrous Histiocytoma; Brain Stem Glioma, Childhood; Brain Tumor, Adult;Brain Tumor, Brain Stem Glioma, Childhood; Brain Tumor, Central NervousSystem Atypical Teratoid/Rhabdoid Tumor, Childhood; Central NervousSystem Embryonal Tumors; Cerebellar Astrocytoma; CerebralAstrocytoma/Malignant Glioma; Craniopharyngioma; Ependymoblastoma;Ependymoma; Medulloblastoma; Medulloepithelioma; Pineal ParenchymalTumors of Intermediate Differentiation; Supratentorial PrimitiveNeuroectodermal Tumors and Pineoblastoma; Visual Pathway andHypothalamic Glioma; Brain and Spinal Cord Tumors; Breast Cancer;Bronchial Tumors; Burkitt Lymphoma; Carcinoid Tumor; Carcinoid Tumor,Gastrointestinal; Central Nervous System Atypical Teratoid/RhabdoidTumor; Central Nervous System Embryonal Tumors; Central Nervous SystemLymphoma; Cerebellar Astrocytoma; Cerebral Astrocytoma/Malignant Glioma,Childhood; Cervical Cancer; Chordoma, Childhood; Chronic LymphocyticLeukemia; Chronic Myelogenous Leukemia; Chronic MyeloproliferativeDisorders; Colon Cancer; Colorectal Cancer; Craniopharyngioma; CutaneousT-Cell Lymphoma; Esophageal Cancer; Ewing Family of Tumors; ExtragonadalGerm Cell Tumor; Extrahepatic Bile Duct Cancer; Eye Cancer, IntraocularMelanoma; Eye Cancer, Retinoblastoma; Gallbladder Cancer; Gastric(Stomach) Cancer; Gastrointestinal Carcinoid Tumor; GastrointestinalStromal Tumor (GIST); Germ Cell Tumor, Extracranial; Germ Cell Tumor,Extragonadal; Germ Cell Tumor, Ovarian; Gestational Trophoblastic Tumor;Glioma; Glioma, Childhood Brain Stem; Glioma, Childhood CerebralAstrocytoma; Glioma, Childhood Visual Pathway and Hypothalamic; HairyCell Leukemia; Head and Neck Cancer; Hepatocellular (Liver) Cancer;Histiocytosis, Langerhans Cell; Hodgkin Lymphoma; Hypopharyngeal Cancer;Hypothalamic and Visual Pathway Glioma; Intraocular Melanoma; Islet CellTumors; Kidney (Renal Cell) Cancer; Langerhans Cell Histiocytosis;Laryngeal Cancer; Leukemia, Acute Lymphoblastic; Leukemia, AcuteMyeloid; Leukemia, Chronic Lymphocytic; Leukemia, Chronic Myelogenous;Leukemia, Hairy Cell; Lip and Oral Cavity Cancer; Liver Cancer; LungCancer, Non-Small Cell; Lung Cancer, Small Cell; Lymphoma, AIDS-Related;Lymphoma, Burkitt; Lymphoma, Cutaneous T-Cell; Lymphoma, Hodgkin;Lymphoma, Non-Hodgkin; Lymphoma, Primary Central Nervous System;Macroglobulinemia, Waldenström; Malignant Fibrous Histiocytoma of Boneand Osteosarcoma; Medulloblastoma; Melanoma; Melanoma, Intraocular(Eye); Merkel Cell Carcinoma; Mesothelioma; Metastatic Squamous NeckCancer with Occult Primary; Mouth Cancer; Multiple Endocrine NeoplasiaSyndrome, (Childhood); Multiple Myeloma/Plasma Cell Neoplasm; MycosisFungoides; Myelodysplastic Syndromes; Myelodysplastic/MyeloproliferativeDiseases; Myelogenous Leukemia, Chronic; Myeloid Leukemia, Adult Acute;Myeloid Leukemia, Childhood Acute; Myeloma, Multiple; MyeloproliferativeDisorders, Chronic; Nasal Cavity and Paranasal Sinus Cancer;Nasopharyngeal Cancer; Neuroblastoma; Non-Small Cell Lung Cancer; OralCancer; Oral Cavity Cancer; Oropharyngeal Cancer; Osteosarcoma andMalignant Fibrous Histiocytoma of Bone; Ovarian Cancer; OvarianEpithelial Cancer; Ovarian Germ Cell Tumor; Ovarian Low MalignantPotential Tumor; Pancreatic Cancer; Pancreatic Cancer, Islet CellTumors; Papillomatosis; Parathyroid Cancer; Penile Cancer; PharyngealCancer; Pheochromocytoma; Pineal Parenchymal Tumors of IntermediateDifferentiation; Pineoblastoma and Supratentorial PrimitiveNeuroectodermal Tumors; Pituitary Tumor; Plasma Cell Neoplasm/MultipleMyeloma; Pleuropulmonary Blastoma; Primary Central Nervous SystemLymphoma; Prostate Cancer; Rectal Cancer; Renal Cell (Kidney) Cancer;Renal Pelvis and Ureter, Transitional Cell Cancer; Respiratory TractCarcinoma Involving the NUT Gene on Chromosome 15; Retinoblastoma;Rhabdomyosarcoma; Salivary Gland Cancer; Sarcoma, Ewing Family ofTumors; Sarcoma, Kaposi; Sarcoma, Soft Tissue; Sarcoma, Uterine; SézarySyndrome; Skin Cancer (Nonmelanoma); Skin Cancer (Melanoma); SkinCarcinoma, Merkel Cell; Small Cell Lung Cancer; Small Intestine Cancer;Soft Tissue Sarcoma; Squamous Cell Carcinoma, Squamous Neck Cancer withOccult Primary, Metastatic; Stomach (Gastric) Cancer; SupratentorialPrimitive Neuroectodermal Tumors; T-Cell Lymphoma, Cutaneous; TesticularCancer; Throat Cancer; Thymoma and Thymic Carcinoma; Thyroid Cancer;Transitional Cell Cancer of the Renal Pelvis and Ureter; TrophoblasticTumor, Gestational; Urethral Cancer; Uterine Cancer, Endometrial;Uterine Sarcoma; Vaginal Cancer; Vulvar Cancer; WaldenströmMacroglobulinemia; or Wilms Tumor.
 8. A method according to claim 1,wherein the metastasis is the metastasis of a cancer chosen from renalcell carcinoma, lung cancer, malignant melanoma, or pancreatic cancer.9.-15. (canceled)
 16. A method according to claim 1, wherein R² is aheteroaryl unit chosen from 1,2,3,4-tetrazol-1-yl,1,2,3,4-tetrazol-5-yl, [1,2,3]triazol-4-yl, [1,2,3]triazol-5-yl,[1,2,4]triazol-4-yl, [1,2,4]triazol-5-yl, imidazol-2-yl, imidazol-4-yl,pyrrol-2-yl, pyrrol-3-yl, oxazol-2-yl, oxazol-4-yl, oxazol-5-yl,isoxazol-3-yl, isoxazol-4-yl, isoxazol-5-yl, [1,2,4]oxadiazol-3-yl,[1,2,4]oxadiazol-5-yl, [1,3,4]oxadiazol-2-yl, furan-2-yl, furan-3-yl,thiophen-2-yl, thiophen-3-yl, isothiazol-3-yl, isothiazol-4-yl,isothiazol-5-yl, thiazol-2-yl, thiazol-4-yl, thiazol-5-yl,[1,2,4]thiadiazol-3-yl, [1,2,4]thiadiazol-5-yl, or[1,3,4]thiadiazol-2-yl. 17.-19. (canceled)
 20. A method according toclaim 1, wherein R⁴ is chosen from methyl, ethyl, n-propyl, iso-propyl,n-butyl, iso-butyl, sec-butyl, and tert-butyl; and R³ is hydrogen.21.-38. (canceled)
 39. A method according to claim 1, wherein thecompound has the formula:

wherein R² is methyl or ethyl, R³ is hydrogen, R^(6a) is chosen fromphenyl, 2-fluorophenyl, 3-fluorophenyl, 4-fluorophenyl,2,3-difluorophenyl, 3,4-difluorophenyl, 3,5-difluorophenyl,2-chlorophenyl, 3-chlorophenyl, 4-chlorophenyl, 2,3-dichlorophenyl,3,4-dichlorophenyl, 3,5-dichlorophenyl, 2-hydroxyphenyl,3-hydroxyphenyl, 4-hydroxyphenyl, 2-methoxyphenyl, 3-methoxyphenyl,4-methoxyphenyl, 2,3-dimethoxyphenyl, 3,4-dimethoxyphenyl,3,5-dimethoxyphenyl, 3-methyl-1,2,4-oxadiazol-5-yl, thiophen-2-yl,thiophen-3-yl, thiazol-2-yl, thiazol-4-yl, thiazol-5-yl, oxazol-2-yl,oxazol-4-yl, oxazol-5-yl, or isoxazol-3-yl.
 40. A method according toclaim 1, wherein the compound has the formula:

wherein R⁴ is methyl, ethyl, phenyl, or thiophen-2-yl, R^(6a) is chosenfrom phenyl, 2-fluorophenyl, 3-fluorophenyl, 4-fluorophenyl,2,3-difluorophenyl, 3,4-difluorophenyl, 3,5-difluorophenyl,2-chlorophenyl, 3-chlorophenyl, 4-chlorophenyl, 2,3-dichlorophenyl,3,4-dichlorophenyl, 3,5-dichlorophenyl, 2-hydroxyphenyl,3-hydroxyphenyl, 4-hydroxyphenyl, 2-methoxyphenyl, 3-methoxyphenyl,4-methoxyphenyl, 2,3-dimethoxyphenyl, 3,4-dimethoxyphenyl,3,5-dimethoxyphenyl, 3-methyl-1,2,4-oxadiazol-5-yl, thiophen-2-yl,thiophen-3-yl, thiazol-2-yl, thiazol-4-yl, thiazol-5-yl, oxazol-2-yl,oxazol-4-yl, oxazol-5-yl, or isoxazol-3-yl. 41.-42. (canceled)
 43. Amethod according to claim 1, wherein the compound has the formula:

or the ammonium salt thereof.
 44. (canceled)
 45. A method according toclaim 1, wherein the compound is chosen from:(S)-4-[2-Benzamido-2-(4-ethylthiazol-2-yl)ethyl]phenylsulfamic acid;(S)-4-{2-(4-Ethylthiazol-2-yl)-2-[2-(2-fluorophenyl)acetamido]ethyl}phenyl-sulfamicacid;(S)-4-(2-(4-Ethylthiazol-2-yl)-2-(2-(3-fluorophenyl)acetamido)ethyl)phenylsulfamicacid;(S)-4-(2-(2-(2,3-Difluorophenyl)acetamido)-2-(4-ethylthiazol-2-yl)ethyl)phenyl-sulfamicacid;(S)-4-(2-(2-(3,4-Difluorophenyl)acetamido)-2-(4-ethylthiazol-2-yl)ethyl)phenyl-sulfamicacid;(S)-4-(2-(2-(2-Chlorophenyl)acetamido)-2-(4-ethylthiazol-2-yl)ethyl)phenylsulfamicacid;(S)-4-(2-(2-(3-Chlorophenyl)acetamido)-2-(4-ethylthiazol-2-yl)ethyl)phenyl-sulfamicacid;(S)-4-(2-(4-Ethylthiazol-2-yl)-2-(2-(3-hydroxyphenyl)acetamido)ethyl)phenyl-sulfamicacid;(S)-4-(2-(4-Ethylthiazol-2-yl)-2-(2-(2-methoxyphenyl)acetamido)ethyl)phenyl-sulfamicacid;(S)-4-(2-(4-Ethylthiazol-2-yl)-2-(2-(3-methoxyphenyl)acetamido)ethyl)phenyl-sulfamicacid;(S)-4-(2-(4-Ethylthiazol-2-yl)-2-(3-phenylpropanamido)ethyl)phenylsulfamicacid;(S)-4-(2-(2-(3,4-Dimethoxyphenyl)acetamido)-2-(4-ethylthiazol-2-yl)ethyl)-phenylsulfamicacid;(S)-4-(2-(2-(2,3-Dimethoxyphenyl)acetamido)-2-(4-ethylthiazol-2-yl)ethyl)-phenylsulfamicacid;(S)-4-(2-(3-(3-Chlorophenyl)propanamido)-2-(4-ethylthiazol-2-yl)ethyl)phenyl-sulfamicacid;(S)-4-(2-(4-Ethylthiazol-2-yl)-2-(3-(2-methoxyphenyl)propanamido)ethyl)phenyl-sulfamicacid;(S)-4-(2-(4-Ethylthiazol-2-yl)-2-(3-(3-methoxyphenyl)propanamido)ethyl)pheny-lsulfamicacid;(S)-4-(2-(4-Ethylthiazol-2-yl)-2-(3-(4-methoxyphenyl)propanamido)ethyl)phenyl-sulfamicacid;(S)-4-{2-[2-(4-Ethyl-2,3-dioxopiperazin-1-yl)acetamido]-2-(4-ethylthiazol-2-yl)ethyl}phenylsulfamicacid;(S)-4-{2-(4-Ethylthiazol-2-yl)-2-[2-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetamide]ethyl}phenylsulfamicacid;(S)-4-[2-(Benzo[d][1,3]dioxole-5-carboxamido)-2-(4-ethylthiazol-2-yl)ethyl]-phenylsulfamicacid;4-((S)-2-(2-(2-Chlorophenyl)acetamido)-2-(2-(thiophene2-yl)thiazol-4-yl)ethyl)-phenylsulfamicacid;4-((S)-2-(2-(3-Methoxyphenyl)acetamido)-2-(2-(thiophene2-yl)thiazol-4-yl)ethyl)-phenylsulfamicacid;4-{(S)-2-(3-Phenylpropanamido)-2-[2-(thiophene2-yl)thiazol-4-yl]ethyl}phenyl-sulfamicacid;4-{(S)-2-(3-(3-Chlorophenyl)propanamido)-2-[2-(thiophene2-yl)thiazol-4-yl]ethyl}-phenylsulfamicacid;4-{(S)-2-[2-(3-Fluorophenyl)acetamide]-2-[2-(thiophene2-yl)thiazol-4-yl]ethyl}-phenylsulfamicacid;(S)-4-{2-[2-(2,5-Dimethylthiazol-4-yl)acetamide]-2-(4-ethylthiazol-2-yl]ethyl}-phenylsulfamicacid;(S)-4-{2-[2-(2,4-Dimethylthiazol-5-yl)acetamide]-2-(4-methylthiazol-2-ylethyl}-phenylsulfamicacid;(S)-4-{2-(4-Ethylthiazol-2-yl)-2-[3-(thiazol-2-yl)propanamido]ethyl}phenylsulfamicacid;(S)-4-{2-(4-Ethylthiazol-2-yl)-2-[2-(4-ethylthiazol-2-yl)acetamide]ethyl}phenyl-sulfamicacid;(S)-4-{2-[2-(3-Methyl-1,2,4-oxadiazol-5-yl)acetamide]-2-(2-phenylthiazol-4-yl)ethyl}phenylsulfamicacid;4-{(S)-2-[2-(4-Ethyl-2,3-dioxopiperazin-1-yl)acetamide]-2-[2-(thiophen-2-yl]thiazol-4-yl)ethyl}phenylsulfamicacid;(S)-4-(2-(2,3-Diphenylpropanamido)-2-(4-ethylthiazol-2-yl)ethyl)phenylsulfamicacid;(S)-4-{2-(4-Ethylthiazol-2-yl)-2-[2-(2-methoxyphenyl)-3-phenylpropanamido]-ethyl}phenylsulfamicacid;(S)-4-{2-(4-Ethylthiazol-2-yl)-2-[2-(3-fluorophenyl)-3-phenylpropanamido]-ethyl}phenylsulfamicacid;(S)-4-{2-(4-Ethylthiazol-2-yl)-2-[2-(3-methoxyphenyl)-3-phenylpropanamido]-ethyl}phenylsulfamicacid;4-{(S)-2-(4-Ethylthiazol-2-yl)-2-[2-(3-methyl-1,2,4-oxadiazol-5-yl)-3-phenylpropanamido]ethyl}phenylsulfamicacid;(S)-4-[2-(4-Ethylthiazol-2-yl)-2-(4-oxo-4-phenylbutanamido)-ethyl]phenylsulfamicacid;(S)-4-(2-(4-Ethylthiazol-2-yl)-2-(5-methyl-4-oxohexanamido)ethyl)phenylsulfamicacid;(S)-4-{2-[4-(3,4-Dihydro-2H-benzo[b][1,4]dioxepin-7-yl)-4-oxobutanamido]-2-(4-ethylthiazol-2-yl)ethyl}phenylsulfamicacid;(S)-4-{2-[4-(2,3-Dimethoxyphenyl)-4-oxobutanamido]-2-(4-ethylthiazol-2-yl)ethyl}phenylsulfamicacid;(S)-4-{2-(4-Ethylthiazol-2-yl)-2-[4-oxo-4-(pyridin-2-yl)butanamido]ethyl}phenyl-sulfamicacid;(S)-4-{2-[4-(2,3-Dihydrobenzo[b][1,4]dioxin-6-yl)-4-oxobutanamido]-2-(4-ethylthiazol-2-yl)ethyl}phenylsulfamicacid;(S)-4-[2-(4-tert-Butoxy-4-oxobutanamido)-2-(4-ethylthiazol-2-yl)ethyl]phenyl-sulfamicacid;(S)-4-[2-(4-Ethoxy-4-oxobutanamido)-2-(4-ethylthiazol-2-yl)ethyl]phenylsulfamicacid;(S)-4-(2-(3-Benzylureido)-2-(4-ethylthiazol-2-yl)ethyl)phenylsulfamicacid;4-{[(S)-2-(2-Ethylthiazol-4-yl)-2-(3-(R)-1methoxy-1-oxo-3-phenylpropan-2-yl)ureido]ethyl}phenylsulfamicacid;4-{(S)-2-(3-Benzylureido)-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid;{4-(S)-[2-Phenylmethanesulfonylamino-2-(2-thiophen-2-ylthiazol-4-yl)ethyl]-phenylsulfamicacid;4-{(S)-2-[(2-Methylthiazol-4-yl)methylsulfonamido]-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid;{4-(S)-[2-Phenylmethanesulfonylamino-2-(2-ethylthiazol-4-yl)ethyl]phenyl}-sulfamicacid;{4-(S)-[2-(3-Methoxyphenyl)methanesulfonylamino-2-(2-ethylthiazol-4-yl)ethyl]phenyl}sulfamicacid;(S)-4-{[1-(2-Ethylthiazol-4-yl)-2-(4-sulfoaminophenyl)ethylsulfamoyl]methyl}-benzoicacid methyl ester;(S)-4-[2-(2-Ethylthiazol-4-yl)-2-(1-methyl-1H-imidazol-4-sulfonamido)ethyl]-phenylsulfamicacid;4-{(S)-2-[2-(Thiophen-2-yl)thiazol-4-yl]-2-(2,2,2-trifluoroethylsulfonamido)-ethyl}phenylsulfamicacid;{4-(S)-[2-(Phenylethanesulfonylamino)-2-(2thiophen-2-ylthiazol-4-yl)ethyl]-phenyl}sulfamicacid;{4-(S)-[3-(Phenylpropanesulfonylamino)-2-(2thiophen-2-ylthiazol-4-yl)ethyl]-phenyl}sulfamicacid;(S)-{4-[2-(4-Methyl-3,4-dihydro-2H-benzo[1,4]oxazine-7-sulfonylamino)-2-(2-thiophen-2-ylthiazol-4-yl)ethyl]phenyl}sulfamicacid;4-{(S)-2-(4-Acetamidophenylsulfonamido)-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid;4-{(S)-2-(2-cyclopropylthiazol-4-yl)-2-[4-(3-methoxyphenyl)-thiazol-2-ylamino]ethyl}phenylsulfamicacid;(S)-4-(2-(4-((2-Methoxy-2-oxoethyl)carbamoyl)thiazole-5-ylamino)2-(2-ethylthiazole-4-yl)ethyl)phenylsulfamicacid;4-((S)-2-(5-(1-N-(2-Methoxy-2-oxoethyl)-1-H-indol-3-yl)oxazole-2-ylamino)-2-(2-methylthiazol-4-yl)ethyl))phenylsulfamicacid;4-((S)-2-(5-(2-Methoxyphenyl)oxazol-2-ylamino)-2-(2-methylthiazol-4-yl)ethyl)-phenylsulfamicacid;4-((5)-2-(5-((S)-1-(tert-Butoxycarbonyl)-2-phenylethyl)oxazole-2-ylamino)-2-(2-methylthiazole-4-yl)ethyl)phenylsulfamicacid;(S)-4-(2-(5-(4-Methoxycarbonyl)phenyl)oxazole-2-ylamino)-2-(2-methylthiazole-4-yl)ethyl)phenylsulfamicacid;(S)-4-(2-(5-(3-Methoxybenzyl)oxazole-2-ylamino)-2-(2-methylthiazole-4-yl)ethyl)-phenylsulfamicacid;(S)-4-(2-(2-Methylthiazole-4-yl)2-(5-phenyloxazole-2-ylamino)ethyl)phenyl-sulfamicacid;4-((S)-2-(2-Cyclopropylthiazol-4-yl)-2-(4-(3-methoxyphenyl)thiazol-2-ylamino)-ethyl)phenylsulfamicacid;(S)-4-(2-(2-cyclopropylthiazol-4-yl)-2-(4-(4-fluorophenyl)thiazol-2-ylamino)ethyl)-phenylsulfamicacid;4-((S)-2-(2-cyclopropylthiazol-4-yl)-2-(4-(2-methoxyphenyl)thiazol-2-ylamino)-ethyl)phenylsulfamicacid;4-((S)-2-(2-cyclopropylthiazol-4-yl)-2-(4-(2,4-difluorophenyl)thiazol-2-ylamino)-ethyl)phenylsulfamicacid;(S)-4-(2-(4-(3-methoxybenzyl)thiazol-2-ylamino)-2-(2-cyclopropylthiazol-4-yl)ethyl)phenylsulfamicacid;(S)-{5-[1-(2-Ethylthiazol-4-yl)-2-(4-sulfoaminophenyl)ethylamino]-2-methyl-2H-[1,2,4]triazole-3-yl}carbamicacid methyl ester;4-{(S)-2-[4-(2-Methoxyphenyl)thiazol-2-ylamino)-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid;4-{(S)-2-[5-(3-Methoxyphenyl)oxazole-2-ylamino]-2-[2-phenylthiazole-4-yl]ethyl}phenylsulfamicacid;4-{(S)-2-[4-(2,4-Difluorophenyl)thiazol-2-ylamino]-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid;(S)-4-{2-[4-(Ethoxycarbonyl)thiazol-2-ylamino]-2-(2-phenylthiazol-4-yl)ethyl}-phenylsulfamicacid;(S)-4-{2-[4-(2-Ethoxy-2-oxoethyl)thiazol-2-ylamino]-2-(2-phenylthiazol-4-yl)ethyl}phenylsulfamicacid;(S)-4-{2-[4-(4-Acetamidophenyl)thiazol-2-ylamino]-2-(2-phenylthiazol-4-yl)ethyl}phenylsulfamicacid;(S)-4-[2-(4-Phenylthiazol-2-ylamino)-2-(2-phenylthiazol-4-yl)ethyl]phenylsulfamicacid;(S)-4-{2-[4-(4-(Methoxycarbonyl)phenyl)thiazol-2-ylamino]-2-(2-phenylthiazol-4-yl)ethyl}phenylsulfamicacid;4-{(S)-2-[4-(Ethoxycarbonyl)thiazol-2-ylamino]-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid;(S)-4-[2-(4-(Methoxycarbonyl)thiazol-5-ylamino)-2-(2-phenylthiazole-4-yl)ethyl]-phenylsulfamicacid;(S)-4-[2-(5-Phenyloxazole-2-ylamino)]-2-(2-phenylthiazole-4-yl)phenylsulfamicacid;(S)-4-{2-[5-(4-Acetamidophenyl)oxazole-2-ylamino]-2-(2-phenylthiazole-4-yl)ethyl}phenylsufamicacid;4-((S)-2-(5-(2,4-Difluorophenyl)oxazole-2-ylamino)-2-(2-phenylthiazole-4-yl)ethyl)phenylsulfamicacid;4-{(S)-2-[5-(3-Methoxyphenyl)oxazol-2-ylamino]-2-[(2-thiophen-2-yl)thiazole-4-yl]ethyl}phenylsulfamicacid;(S)-4-[2-(4,6-Dimethylpyrimidene-2-ylamino)-2-(2-methylthiazole-4-yl)ethyl]-phenylsulfamicacid;(S)-4-[2-(4-Hydroxy-6-methylpyrimidine-2-ylamino)-2-(2-methylthiazole-4-yl)ethyl]phenylsulfamicacid;4-{(S)-2-[(S)-2-(tert-Butoxycarbonylamino)-3-phenylpropanamido]-2-(4-ethylthiazol-2-yl)ethyl}phenylsulfamicacid;4-{(S)-2-[(R)-2-(tert-Butoxycarbonylamino)-3-phenylpropanamido]-2-(4-ethylthiazol-2-yl)ethyl}phenylsulfamicacid;{1-[1-(5-Ethylthiazol-2-yl)-(S)-2-(4-sulfoaminophenyl)ethylcarbamoyl]-(S)-2-phenylethyl}methylcarbamic acid tert-butyl ester;{(S)-2-Phenyl-1-[1-(4-phenylthiazol-2-yl)-(S)-2-(4-sulfoaminophenyl)ethyl-carbamoyl]ethyl}carbamicacid tert-butyl ester;4-{(S)-2-(S)-2-(tert-Butoxycarbonylamino)-3-phenylpropaneamido-2-(2-phenylthiazole-4-yl)}phenylsulfamicacid;4-{(S)-2-(4-Ethylthiazol-2-yl)-2-[(S)-2-(methoxycarbonylamino)-3-phenyl-propanamido]ethyl}phenylsulfamicacid;4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-(thiazol-2-yl)ethyl}phenylsulfamicacid;4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-(4-methylthiazol-2-yl)ethyl}phenylsulfamicacid;4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-(4-propylthiazol-2-yl)ethyl}phenylsulfamicacid;4-{(S)-2-(4-tert-Butylthiazol-2-yl)-2-[(S)-2-(methoxycarbonylamino)-3-phenylpropanamido]ethyl}phenylsulfamicacid;4-{(S)-2-(4-Cyclopropylthiazol-2-yl)-2-[(S)-2-(methoxycarbonylamino)-3-phenylpropanamido]ethyl}phenylsulfamicacid;4-{(S)-2-(4-Cyclohexylthiazol-2-yl)-2-[(S)-2-(methoxycarbonylamino)-3-phenyl-propanamido]ethyl}phenylsulfamicacid;4-{(S)-2-(4,5-Dimethylthiazol-2-yl)-2-[(S)-2-(methoxycarbonylamino)-3-phenyl-propanamido]ethyl}phenylsulfamicacid;4-{(S)-2-Phenyl-1-[1-(2-phenylthiazol-4-yl)-(S)-2-(4-sulfoaminophenyl)ethyl-carbamoyl]ethyl}carbamicacid tert-butyl ester;4-{(S)-2-(4-Ethyl-5-methylthiazol-2-yl)-2-[(S)-2-(methoxycarbonylamino)-3-phenyl-propanamido]ethyl}phenylsulfamicacid;4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-[4-(2,2,2-trifluoroethyl)thiazol-2-yl]ethyl}phenylsulfamicacid;4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido)-2-[4-(3,3,3-trifluoropropyl)thiazol-2-yl]ethyl}phenylsulfamicacid;4-{(S)-2-[4-(2,2-Difluorocyclopropyl)thiazol-2-yl]-2-[(S)-2-(methoxycarbonylamino)-3-phenylpropanamido]ethyl}phenylsulfamicacid;4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-[4-(methoxy-methyl)thiazol-2-yl]ethyl}phenylsulfamicacid;4-{(S)-2-(4-(Ethoxycarbonylamino)thiazol-2-yl)-2-[(S)-2-(methoxycarbonylamino)-3-phenylpropanamido]ethyl}phenylsulfamicacid;4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-(5-phenylthiazol-2-yl))ethyl}phenylsulfamicacid;4-{(S)-2-(4-tert-Butylthiazol-2-yl)-2-[(S)-2-(methoxycarbonylamino)-3-phenyl-propanamido]ethyl}phenylsulfamicacid;4-{(S)-2-(4-Ethyl-5-phenylthiazol-2-yl)-2-[(S)-2-(methoxycarbonylamino)-3-phenyl-propanamido]ethyl}phenylsulfamicacid;4-{(S)-2-[4-(3,4-Dimethylphenyl)thiazol-2-yl]-2-[(S)-2-(methoxycarbonylamino)-3-phenylpropanamido]ethyl}phenylsulfamicacid;4-{(S)-2-[4-(4-Chlorophenyl)thiazol-2-yl]-2-[(S)-2-(methoxycarbonylamino)-3-phenylpropanamido]ethyl}phenylsulfamicacid;4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-(4-phenylthiazol-2-yl)ethyl}phenylsulfamicacid;4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-[4-(thiophen-2-yl)thiazol-2-yl]ethyl}phenylsulfamicacid;4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-[4-(thiophen-3-yl)thiazol-2-yl]ethyl}phenylsulfamicacid;4-{(S)-2-(4-Ethylthiazol-2-yl)-2-[(S)-2-(methoxycarbinyl)-3-phenylpropion-amido]ethyl}phenylsulfamicacid;4-{(S)-2-(5,6-Dihydro-4H-cyclopenta[d]thiazol-2-yl)-2-[(S)-2-(methoxy-carbonyl)-3-phenylpropanamido]ethyl}phenylsulfamicacid;4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-(4,5,6,7-tetrahydrobenzo[d]thiazol-2-yl)ethyl}phenylsulfamicacid;4-{(S)-2-[4-(5-Chlorothiophen-2-yl)thiazol-2-yl]-2-[(S)-2-(methoxycarbonylamino)-3-phenylpropanamido]ethyl}phenylsulfamicacid;4-{(S)-2-[(S)-2-(Ethoxycarbonylamino)-3-phenylpropanamido]-2-(4-ethylthiazol-2-yl)ethyl}phenylsulfamicacid;4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-(2-ethylthiazol-4-yl)ethyl}phenylsulfamicacid;4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-(2-methyl-thiazol-4-yl)ethyl}phenylsulfamicacid;4-{(S)-2-(2-Ethylthiazole-4-yl)-2-[(S)-2-(methoxycarbonylamino)-3-phenylpropan-amido]ethyl}phenylsulfamicacid;4-{(S)-2-(2-Isopropylthiazol-4-yl)-2-[(S)-2-(methoxycarbonylamino)-3-phenyl-propan-amido]ethyl}phenylsulfamicacid;4-{(S)-2-(2-Cyclopropylthiazol-4-yl)-2-[(S)-2-(methoxycarbonylamino)-3-phenylpropanamido]ethyl}phenylsulfamicacid;4-{(S)-2-{2-[(4-Chlorophenylsulfonyl)methyl]thiazol-4-yl}-2-[(S)-2-(methoxy-carbonyl)-3-phenylpropanamido]ethyl}phenylsulfamicacid;4-{(S)-2-[2-(tert-Butylsulfonylmethyl)thiazol-4-yl]-2-[(S)-2-(methoxycarbonylamino)-3-phenylpropanamido]ethyl}phenylsulfamicacid;4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropionamido]-2-(2-phenyl-thiazole-4-yl)ethyl}phenylsulfamicacid;4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid;4-{(S)-2-[2-(3-Chlorothiophen-2-yl)thiazol-4-yl]-2-[(S)-2-(methoxycarbonylamino)-3-phenylpropanamido]ethyl}phenylsulfamicacid;4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-[2-(3-methylthiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid;4-{[(S)-2-(2-(Furan-2-yl)thiazol-4-yl]-2-[(S)-2-(methoxycarbonylamino)-3-phenyl-propanamido]ethyl}phenylsulfamicacid;4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-[2-(2-methyl-thiazole-4-yl)thiazol-4-yl]ethyl}phenylsulfamicacid;4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-(2-pyrazine-2-yl)thiazole-4-yl}phenylsulfamicacid;4-{(S)-2-[(S)-2-(Methoxycarbonylamino)-3-phenylpropanamido]-2-[2-(6-methyl-pyridin-3-yl)thiazol-4-yl]ethyl}phenylsulfamicacid;4-[(S)-2-((S)-2-Acetamido-3-phenylpropanamido)-2-(4-ethylthiazol-2-yl)ethyl]-phenylsulfamicacid;4-[(S)-2-((S)-2-Acetamido-3-phenylpropanamido)-2-(4-tert-butylthiazol-2-yl)ethyl]-phenylsulfamicacid;4-{(S)-2-((S)-2-Acetamido-3-phenylpropanamido)-2-[4-(thiophen-3-yl)thiazol-2-yl]ethyl)phenylsulfamicacid;4-{(S)-2-[(S)-2-(tert-Butoxycarbonyl)-3-methylbutanamido]-2-(4-ethylthiazol-2-yl)ethyl}phenylsulfamicacid;(S)-4-{2-[2-(tert-Butoxycarbonyl)acetamide]-2-(4-ethylthiazol-2-yl)ethyl}phenyl-sulfamicacid;(S)-4-{2-(4-Ethylthiazol-2-yl)-2-[2-(methoxycarbonyl)acetamido]ethyl}phenyl-sulfamicacid;4-{(S)-2-(4-Ethylthiazol-2-yl)-2-[(S)-2-(methoxycarbonyl)-3-methylbutanamido]-ethyl}phenylsulfamicacid;4-{(S)-2-[(S)-2-(tert-Butoxycarbonyl)-4-methylpentanamido]-2-(4-ethylthiazol-2-yl)ethyl}phenylsulfamicacid;4-{(S)-2-(4-Ethylthiazol-2-yl)-2-[(S)-2-(methoxycarbonyl)-4-methylpentan-amido]ethyl}phenylsulfamicacid;4-((S)-2-(4-Ethylthiazol-2-yl)-2-{(S)-2-[2-(methoxycarbonyl)acetamide]-3-phenylpropanamido}ethyl)phenylsulfamicacid;4-{(S)-2-[(S)-2-(tert-Butoxycarbonyl)-4-methylpentanamido]-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid;4-{(S)-2-[(S)-2-(Methoxycarbonyl)-4-methylpentanamido]-2-[2-(thiophen-2-yl)thiazol-4-yl]ethyl}phenylsulfamicacid; and(S)-4-{2-[2-(tert-Butoxycarbonyl)acetamide]-2-(4-ethylthiazol-2-yl)ethyl}-phenylsulfamicacid.
 46. A method according to claim 1, wherein the compound has theformula:

and pharmaceutically acceptable salts thereof.
 47. (canceled)
 48. Amethod according to claim 1, wherein the compound is in the form of asalt of a cation chosen from ammonium, sodium, lithium, potassium,calcium, magnesium, bismuth, and lysine.
 49. A composition for treatingcancer in a subject, comprising: a) an effective amount of one or morecompounds having the formula:

wherein R is a substituted or unsubstituted thiazolyl unit having theformula:

R², R³, and R⁴ are each independently: i) hydrogen; ii) substituted orunsubstituted C₁-C₆ linear, branched, or cyclic alkyl; iii) substitutedor unsubstituted C₂-C₆ linear, branched, or cyclic alkenyl; iv)substituted or unsubstituted C₂-C₆ linear or branched alkynyl; v)substituted or unsubstituted C₆ or C₁₀ aryl; vi) substituted orunsubstituted C₁-C₉ heteroaryl; vii) substituted or unsubstituted C₁-C₉heterocyclic; or viii) R² and R³ can be taken together to form asaturated or unsaturated ring having from 5 to 7 atoms; wherein from 1to 3 atoms can optionally be heteroatoms chosen from oxygen, nitrogen,and sulfur; Z is a unit having the formula:-(L)_(n)-R¹ R¹ is chosen from: i) hydrogen; ii) hydroxyl; iii) amino;iv) substituted or unsubstituted C₁-C₆ linear, branched or cyclic alkyl;v) substituted or unsubstituted C₁-C₆ linear, branched or cyclic alkoxy;vi) substituted or unsubstituted C₆ or C₁₀ aryl; vii) substituted orunsubstituted C₁-C₉ heterocyclic rings; or viii) substituted orunsubstituted C₁-C₉ heteroaryl rings; L is a linking unit having theformula:-[Q]_(y)[C(R^(5a)R^(5b))]_(x)[Q¹]_(z)[C(R^(6a)R^(6b))]_(w)- Q and Q¹ areeach independently: i) —C(O)—; ii) —NH—; iii) —C(O)NH—; iv) —NHC(O)—; v)—NHC(O)NH—; vi) —NHC(O)O—; vii) —C(O)O—; viii) —C(O)NHC(O)—; ix) —O—; x)—S—; xi) —SO₂—; xii) —C(═NH)—; xiii) —C(═NH)NH—; xiv) —NHC(═NH)—; or xv)—NHC(═NH)NH—; R^(5a) and R^(5b) are each independently: i) hydrogen; ii)hydroxy; iii) halogen; iv) C₁-C₆ substituted or unsubstituted linear orbranched alkyl; or v) a unit having the formula:—[C(R^(7a)R^(7b))]_(t)R⁸ R^(7a) and R^(7b) are each independently: i)hydrogen; or ii) substituted or unsubstituted C₁-C₆ linear, branched, orcyclic alkyl; R⁸ is: i) hydrogen; ii) substituted or unsubstituted C₁-C₆linear, branched, or cyclic alkyl; iii) substituted or unsubstituted C₆or C₁₀ aryl; iv) substituted or unsubstituted C₁-C₉ heteroaryl; or v)substituted or unsubstituted C₁-C₉ heterocyclic; R^(6a) and R^(6b) areeach independently: i) hydrogen; or ii) C₁-C₄ linear or branched alkyl;the index n is 0 or 1; the indices t, w and x are each independentlyfrom 0 to 4; the indices y and z are each independently 0 or 1; or apharmaceutically acceptable salt thereof; and b) an effective amount ofone or more chemotherapeutic agents; wherein the one or more compoundsand the chemotherapeutic agents can be administered together or in anyorder.
 50. A composition according to claim 49, wherein thechemotherapeutic agent is chosen from taxol, IL-2, gemcitabine,erlotinib, doxil, irinortecan, and bevacizumab.
 51. A compositionaccording to claim 49, wherein the compound has the formula:

and pharmaceutically acceptable salts thereof.
 52. A compositionaccording to claim 49, wherein the compound is in the form of a salt ofa cation chosen from ammonium, sodium, lithium, potassium, calcium,magnesium, bismuth, and lysine.
 53. (canceled)
 54. A compositionaccording to claim 49, wherein the cancer is renal cell carcinoma, lungcancer, malignant melanoma, or pancreatic cancer.
 55. A method accordingto claim 2, wherein the cancer is renal cell carcinoma, lung cancer,malignant melanoma, or pancreatic cancer.
 56. A method according toclaim 3, wherein the cancer is renal cell carcinoma, lung cancer,malignant melanoma, or pancreatic cancer.
 57. A method according toclaim 4, wherein the metastasis is metastasis of a cancer chosen fromrenal cell carcinoma, lung cancer, malignant melanoma, or pancreaticcancer.
 58. A method according to claim 1, wherein the compound ischosen from a compound having the formula: i)

ii)

and iii)

or the ammonium salt thereof.
 59. A method according to claim 2, whereinthe compound is chosen from a compound having the formula: i)

ii)

and iii)

or the ammonium salt thereof.
 60. A method according to claim 3, whereinthe compound is chosen from a compound having the formula: i)

ii)

and iii)

or the ammonium salt thereof.
 61. A method according to claim 4, whereinthe compound is chosen from a compound having the formula: i)

ii)

and iii)

or the ammonium salt thereof.